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The map label for this gene is oprM [H]

Identifier: 187736522

GI number: 187736522

Start: 2484591

End: 2486024

Strand: Direct

Name: oprM [H]

Synonym: Amuc_2043

Alternate gene names: 187736522

Gene position: 2484591-2486024 (Clockwise)

Preceding gene: 187736521

Following gene: 187736523

Centisome position: 93.26

GC content: 61.16

Gene sequence:

>1434_bases
ATGCGAACAAACCCATACATCACACGCGCCATCCTGCTGGGAACGGCTTTTGCCGCCTCCTCCTGCATGATGGGCCCCGA
CTTCAAACCGGTGGACATGCCCATGCCTGCGGCATTCAGGGGAGCCCCTGCAGCGACGGAATCCATTGCGGACCTTCCCT
GGTGGAAAGTTTTCAAAAACAAGGACCTTCAGGACCTCCTGACGGACACCTACAATAACAACCGCGATTTGAAAGCTACC
ATGGCGCGCGTGGAAAAGGCACGCCAGTACATCACCATCACGGAGGCCCCGCTCTTCCCGTGGGCGGATTATTCCGGCTC
CGTCAGCAAAGGCTCCAACTATACCGGCGGCAGCATCGCCCAGACTACCGGAACCACACTGACGCCTGGAGCGATTGATG
CCGGCATTTCCTGGGAACTGGATATCTGGGGCAAAACGCGCCGGATGACGGAAGCGGCCCGCGCGGATTATCTGGCTTCC
GACGAAGGCCAGCGCGCGCTCATGCTTTCCCTGCTCCGCCAGGTGGCGGACTCCTACCTCCAGCTCCTCCAGCTGGACGA
ACAGCTTGCCATCGTGCAGAAATCCGTGGAATCCTATTCCGAAAGCCTGCGCCTGTTTGACGAACAGCTTGAAGGCCAGG
TAGGCGACAGGCTTCAGGTGGCTTCCGCCAAGGCGGCTCTGGCCTCCTCCCAGGCCCAGATTCCCGCCATTCAGGTGCAA
ATTGCCAATCTGGAAAATGCAGTCTCCGTCCTGGCCGGACGCGCTCCCGGCCATATCCGCCGTTCCGGCAGCACCCGGGA
CATCGCCTATAACGTCAAGGTTCCCGCCGGCATTCCGGCCTACATCCTTTCCAGAAGGCCTGACGTCCGCCAGAGCGAAT
ACCAGCTGCGCGCCGCCAATGCGGAAGTGGGCGTGGCCATTGCCAATTACTTCCCGACCATCTCCCTGACGGCGGCGGGC
GGCCTCGCCTCCGCGGATCTGCGCCACGTGCAGGGGCGCCGCGGCGGCTGGGGCCTGGGAGCCAATCTGACCGGCCCCCT
CTTCCAGGCGGGCAAGCTGACGGCTTCCGAAAAGGCGGCCAAGGCCGAATTCCTGGCGGCCAGAAACGATTATGAACAAA
CGGTTCTCAATGCCCTGGCGGAAGTTTCCAGCACGCTTATCCAGAGGGACAAGCTGCGCAGCATCACCGCCACCCAGTCC
GAGGCCGTGGAAGCCTACCACACGGCGGTGAAACTCTCCTTTGAACGCTACCGCACGGGCCTTTCCAATTACATTGAAGT
ATTGTACGCCCAGCAGAACCTGTACCCCGCTCAGATTCAGCTTTCACAGTATTATTACCAGCACGCCAGCACGCTGGTTT
CCCTGTACACGGCCCTGGGCGGCGGCTGGAACATGAGCCACAAGGCTATCATGGACGGCCCTGCCAGGCAGTAA

Upstream 100 bases:

>100_bases
GGACGAAATCATCGCCCGCAAACACCTGTTCCACGAAGCCCATGAATCATTAAAAGGGTAAAACCGCCCCTGCAACCATC
TAAAAACAGACTAAACGCAT

Downstream 100 bases:

>100_bases
AACGCGCCCCTTAAAACAGGGAATTTTCCTGACTGTTTTTTCATCCTCCGGCAAGTACGGCTTGCCGGAGGATTTGTTTT
CCGGCCATGGGGAAAACGCC

Product: RND efflux system, outer membrane lipoprotein, NodT family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRTNPYITRAILLGTAFAASSCMMGPDFKPVDMPMPAAFRGAPAATESIADLPWWKVFKNKDLQDLLTDTYNNNRDLKAT
MARVEKARQYITITEAPLFPWADYSGSVSKGSNYTGGSIAQTTGTTLTPGAIDAGISWELDIWGKTRRMTEAARADYLAS
DEGQRALMLSLLRQVADSYLQLLQLDEQLAIVQKSVESYSESLRLFDEQLEGQVGDRLQVASAKAALASSQAQIPAIQVQ
IANLENAVSVLAGRAPGHIRRSGSTRDIAYNVKVPAGIPAYILSRRPDVRQSEYQLRAANAEVGVAIANYFPTISLTAAG
GLASADLRHVQGRRGGWGLGANLTGPLFQAGKLTASEKAAKAEFLAARNDYEQTVLNALAEVSSTLIQRDKLRSITATQS
EAVEAYHTAVKLSFERYRTGLSNYIEVLYAQQNLYPAQIQLSQYYYQHASTLVSLYTALGGGWNMSHKAIMDGPARQ

Sequences:

>Translated_477_residues
MRTNPYITRAILLGTAFAASSCMMGPDFKPVDMPMPAAFRGAPAATESIADLPWWKVFKNKDLQDLLTDTYNNNRDLKAT
MARVEKARQYITITEAPLFPWADYSGSVSKGSNYTGGSIAQTTGTTLTPGAIDAGISWELDIWGKTRRMTEAARADYLAS
DEGQRALMLSLLRQVADSYLQLLQLDEQLAIVQKSVESYSESLRLFDEQLEGQVGDRLQVASAKAALASSQAQIPAIQVQ
IANLENAVSVLAGRAPGHIRRSGSTRDIAYNVKVPAGIPAYILSRRPDVRQSEYQLRAANAEVGVAIANYFPTISLTAAG
GLASADLRHVQGRRGGWGLGANLTGPLFQAGKLTASEKAAKAEFLAARNDYEQTVLNALAEVSSTLIQRDKLRSITATQS
EAVEAYHTAVKLSFERYRTGLSNYIEVLYAQQNLYPAQIQLSQYYYQHASTLVSLYTALGGGWNMSHKAIMDGPARQ
>Mature_477_residues
MRTNPYITRAILLGTAFAASSCMMGPDFKPVDMPMPAAFRGAPAATESIADLPWWKVFKNKDLQDLLTDTYNNNRDLKAT
MARVEKARQYITITEAPLFPWADYSGSVSKGSNYTGGSIAQTTGTTLTPGAIDAGISWELDIWGKTRRMTEAARADYLAS
DEGQRALMLSLLRQVADSYLQLLQLDEQLAIVQKSVESYSESLRLFDEQLEGQVGDRLQVASAKAALASSQAQIPAIQVQ
IANLENAVSVLAGRAPGHIRRSGSTRDIAYNVKVPAGIPAYILSRRPDVRQSEYQLRAANAEVGVAIANYFPTISLTAAG
GLASADLRHVQGRRGGWGLGANLTGPLFQAGKLTASEKAAKAEFLAARNDYEQTVLNALAEVSSTLIQRDKLRSITATQS
EAVEAYHTAVKLSFERYRTGLSNYIEVLYAQQNLYPAQIQLSQYYYQHASTLVSLYTALGGGWNMSHKAIMDGPARQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=459, Percent_Identity=30.718954248366, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI2367348, Length=490, Percent_Identity=23.6734693877551, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51961; Mature: 51961

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTNPYITRAILLGTAFAASSCMMGPDFKPVDMPMPAAFRGAPAATESIADLPWWKVFKN
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHCCCHHHHHCC
KDLQDLLTDTYNNNRDLKATMARVEKARQYITITEAPLFPWADYSGSVSKGSNYTGGSIA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
QTTGTTLTPGAIDAGISWELDIWGKTRRMTEAARADYLASDEGQRALMLSLLRQVADSYL
ECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QLLQLDEQLAIVQKSVESYSESLRLFDEQLEGQVGDRLQVASAKAALASSQAQIPAIQVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCCEEEEE
IANLENAVSVLAGRAPGHIRRSGSTRDIAYNVKVPAGIPAYILSRRPDVRQSEYQLRAAN
ECCHHHHHHHHHCCCCCCEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCHHHEEECC
AEVGVAIANYFPTISLTAAGGLASADLRHVQGRRGGWGLGANLTGPLFQAGKLTASEKAA
CCCEEEEEHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCHHHHHH
KAEFLAARNDYEQTVLNALAEVSSTLIQRDKLRSITATQSEAVEAYHTAVKLSFERYRTG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSNYIEVLYAQQNLYPAQIQLSQYYYQHASTLVSLYTALGGGWNMSHKAIMDGPARQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MRTNPYITRAILLGTAFAASSCMMGPDFKPVDMPMPAAFRGAPAATESIADLPWWKVFKN
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHCCCHHHHHCC
KDLQDLLTDTYNNNRDLKATMARVEKARQYITITEAPLFPWADYSGSVSKGSNYTGGSIA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
QTTGTTLTPGAIDAGISWELDIWGKTRRMTEAARADYLASDEGQRALMLSLLRQVADSYL
ECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QLLQLDEQLAIVQKSVESYSESLRLFDEQLEGQVGDRLQVASAKAALASSQAQIPAIQVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCCEEEEE
IANLENAVSVLAGRAPGHIRRSGSTRDIAYNVKVPAGIPAYILSRRPDVRQSEYQLRAAN
ECCHHHHHHHHHCCCCCCEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCHHHEEECC
AEVGVAIANYFPTISLTAAGGLASADLRHVQGRRGGWGLGANLTGPLFQAGKLTASEKAA
CCCEEEEEHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCHHHHHH
KAEFLAARNDYEQTVLNALAEVSSTLIQRDKLRSITATQSEAVEAYHTAVKLSFERYRTG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSNYIEVLYAQQNLYPAQIQLSQYYYQHASTLVSLYTALGGGWNMSHKAIMDGPARQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]