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The map label for this gene is mdtK [H]

Identifier: 187736515

GI number: 187736515

Start: 2472372

End: 2473766

Strand: Reverse

Name: mdtK [H]

Synonym: Amuc_2036

Alternate gene names: 187736515

Gene position: 2473766-2472372 (Counterclockwise)

Preceding gene: 187736516

Following gene: 187736514

Centisome position: 92.86

GC content: 59.21

Gene sequence:

>1395_bases
ATGGAAGGGAATAAAAAAATGAATAAGACGTGGAATTTGAAGGAAATGAAAAAGTTGATACCGCTGGCCCTTCCGGTGCT
GGTGGTCAACCTCTCCATTGTGGGCATGGGGGCGGTGGACGCTATTGTGGCCGGGCGCGCCGGCGTGACGGACATGGCCG
CCGTGGCCCTGGGGTCTTCCGTGTACCTGCCCGTGGCTCTCTTCGCCTGCGGCGTGTTGATGATCATCGGCCCCGTAATT
GCCAACATGCGGGGGAAAAGCCATGAAAGCCGCGTGGGCTACATGACCAACCACGGCCTGTGGCTGGCGTTCATGCTCAG
TCTGGTTTCCATGCCGGTCATTTATGTGTTGAGAAATGTGTTCGGCTGGATTTCCGATGACGCCGCCATGTGCCAGATGG
CCTCCGCCTACATGTTCGCCATTATGTGGGGCCTTCCCGCCAACCTGGGGTTCGTGGCCCTGAAGAGCCTGAACGAAGGC
TCCAACATGACACGTCCCGCCATGTACGTGGGATTGTGCGGCCTGTTGCTCAACATTCCCCTGAACTACATGTTCGTCTT
TGGCATGTACGGTTTTCCCCGCATGGGTGGAGCGGGGTGCGGTGCGGCCACGGCAGTCATTTTCTACATTGAATTCCTGC
TGATGTTCCTGCTGGTTTACTTCAATCCCAAGCACAGGCCGTACCGCAGGCACATCATTTCCTGGCGGCGGCCTACGCCT
TCCGTCATCACGCACCTGGTGCGGCTGGGCGTGCCTATAGGCGTTTCCCAGCTGTGCGAGGTGATGCTCTTCTGCGCGGC
GGCTCTGGTGCTGGCTCCGCTGGGAGAGACGCAGGTGGCTAGCCACCAGATTGCCGGGAACGTGGGCGGCCTGGTGTTCA
TGCTCCCGCTTTCCGTAGGGCTGGCGGCTTCCATCCGCGTGGCGTACCATCACGGCAGGAATGACCTGGCAGGCACCAGA
TCCGCCATTCTGTCTTCCTATGTGCTGGTGCTCACCATCTGTCTGTGCACCTTTGGAGGCATCACCCTGTTCCGCGAGCA
GATCGTGCACCTGTACAATGACTCGGAGCTGATTGTCAGCACGGCTTCCGTCCTGCTGGTTCTGGCGGCGGCCTACCAGC
TTCCGGACTGTTTGCAGGTGCTTTCCGTCGGGGTTCTGAGAGGATTCCGGGATACGGCGTCCATTACCTTCATTACGTTT
TTCTCTTATTGGATGGTAGGATTTCCGGCGTGCTACATCCTTGCCCGTACGGACTGGATTGTCCCGGCCATGGGAGCGCG
GGGCATCTGGACGGGATTCATCATCGGCCTGGCAGTAGCGGCGGTGCTGCTGCTCTGGCGCGTAAGGCGCACTACCAGGC
GGGAATTTTCCCTGATGAGGCAGGCGGGGGAATAA

Upstream 100 bases:

>100_bases
CCGGTACGGTTGGCAACCTTTAACTTTAAAGCCGGATTGGAGCCTGCCGCTCTGGTCCGGCTTGTTTGCCGGCATGTTCC
GGCTTTTTGAGTGAGGATGC

Downstream 100 bases:

>100_bases
CTTTCATCTTCTTCATTCCCGATATTAAAAGGGCGGCTTTCTGGGAGAATTAAGTTTCCTAAGCATTTCCCTTCCGAGAG
GAGGAATGCGATGTTCCGGA

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MEGNKKMNKTWNLKEMKKLIPLALPVLVVNLSIVGMGAVDAIVAGRAGVTDMAAVALGSSVYLPVALFACGVLMIIGPVI
ANMRGKSHESRVGYMTNHGLWLAFMLSLVSMPVIYVLRNVFGWISDDAAMCQMASAYMFAIMWGLPANLGFVALKSLNEG
SNMTRPAMYVGLCGLLLNIPLNYMFVFGMYGFPRMGGAGCGAATAVIFYIEFLLMFLLVYFNPKHRPYRRHIISWRRPTP
SVITHLVRLGVPIGVSQLCEVMLFCAAALVLAPLGETQVASHQIAGNVGGLVFMLPLSVGLAASIRVAYHHGRNDLAGTR
SAILSSYVLVLTICLCTFGGITLFREQIVHLYNDSELIVSTASVLLVLAAAYQLPDCLQVLSVGVLRGFRDTASITFITF
FSYWMVGFPACYILARTDWIVPAMGARGIWTGFIIGLAVAAVLLLWRVRRTTRREFSLMRQAGE

Sequences:

>Translated_464_residues
MEGNKKMNKTWNLKEMKKLIPLALPVLVVNLSIVGMGAVDAIVAGRAGVTDMAAVALGSSVYLPVALFACGVLMIIGPVI
ANMRGKSHESRVGYMTNHGLWLAFMLSLVSMPVIYVLRNVFGWISDDAAMCQMASAYMFAIMWGLPANLGFVALKSLNEG
SNMTRPAMYVGLCGLLLNIPLNYMFVFGMYGFPRMGGAGCGAATAVIFYIEFLLMFLLVYFNPKHRPYRRHIISWRRPTP
SVITHLVRLGVPIGVSQLCEVMLFCAAALVLAPLGETQVASHQIAGNVGGLVFMLPLSVGLAASIRVAYHHGRNDLAGTR
SAILSSYVLVLTICLCTFGGITLFREQIVHLYNDSELIVSTASVLLVLAAAYQLPDCLQVLSVGVLRGFRDTASITFITF
FSYWMVGFPACYILARTDWIVPAMGARGIWTGFIIGLAVAAVLLLWRVRRTTRREFSLMRQAGE
>Mature_464_residues
MEGNKKMNKTWNLKEMKKLIPLALPVLVVNLSIVGMGAVDAIVAGRAGVTDMAAVALGSSVYLPVALFACGVLMIIGPVI
ANMRGKSHESRVGYMTNHGLWLAFMLSLVSMPVIYVLRNVFGWISDDAAMCQMASAYMFAIMWGLPANLGFVALKSLNEG
SNMTRPAMYVGLCGLLLNIPLNYMFVFGMYGFPRMGGAGCGAATAVIFYIEFLLMFLLVYFNPKHRPYRRHIISWRRPTP
SVITHLVRLGVPIGVSQLCEVMLFCAAALVLAPLGETQVASHQIAGNVGGLVFMLPLSVGLAASIRVAYHHGRNDLAGTR
SAILSSYVLVLTICLCTFGGITLFREQIVHLYNDSELIVSTASVLLVLAAAYQLPDCLQVLSVGVLRGFRDTASITFITF
FSYWMVGFPACYILARTDWIVPAMGARGIWTGFIIGLAVAAVLLLWRVRRTTRREFSLMRQAGE

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=39.8249452954048, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI226510954, Length=455, Percent_Identity=22.8571428571429, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50627; Mature: 50627

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA