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The map label for this gene is pycB [H]

Identifier: 187736513

GI number: 187736513

Start: 2469820

End: 2471343

Strand: Direct

Name: pycB [H]

Synonym: Amuc_2034

Alternate gene names: 187736513

Gene position: 2469820-2471343 (Clockwise)

Preceding gene: 187736512

Following gene: 187736519

Centisome position: 92.71

GC content: 60.89

Gene sequence:

>1524_bases
ATGAATCCTGTTACATTCAATTGCACCGTTCTGCGCGACGGGCATCAATCCCTGGCGGCAACCCGCATGAAGACGGCAGA
CATGCTGCCCATCGCCCCCATTCTGGACTCCATGGGCTTCAGCGCCCTGGAAACCTGGGGCGGCGCCACCATCGACGCCG
GGCTGCGCTTCTTGAAAGAATGGCCTTTCGACCGGCTGGACGCCCTGAAAAAAGCGGCCCCCAAGACGCCGCACATGATG
CTGCTGCGCGGTCAGAATATCGTGGGCTACACCAACTATGCGGACGACGTGGTGGAAGCCTTTGTCGCCATGAGCGCCAA
GCACGGCATGAACATCTTCCGCATTTTCGACTGCGTGAACGACCCGCGCAACATGGAAACCTCCATCCGCGCCGCCAAGA
AAGCCGGAGCTCAGGCCCACGGCACCATCTGCTACACGACTTCTCCCGTGCACACCACGCAGACTTTTGTGGACATGGGG
CGGGAACTCGCGGATATGGGAGCGGACGCCATCGTCATCAAGGATATGGCCGGCCTCATTCCTCCGTATGTCACCCAGGA
ACTGGTCAGCGCCCTGAAGAAAGACCTGAACATCCCCGTCTGGATCCACACGCATGACACTGCCGGCCTCGGCGCCTCCA
CCTACCTCAGCGCCATTGATGCCGGAGTGGACGCGTGCGATGTCTCCATCTCCCCCTTCGCCAACGGCACGGGCCAGCCG
GACTGCCTGCGCATGCTTGCCTTGCTGAACGGCAATCCCCGCAAGCCGGATTACGACGCGGACAAGCTCATTGAAGTTTC
CGAAATGCTCAAACCCGTCTATGAAGGTCTGGGCAAATTCGCCTCCCACCGCAACGAAGTGGTGGACTCCGACACGCTCC
GCTACCAGGTCCCCGGCGGCATGCTTTCCAACTTCCGCACCCAGCTCAAGGAACAGGGCATGGAAGACAAATTTGAGGAA
GTATTCGCGGAAATCCCGGTAGTCCGCAAGGCTCTGGGCTGGATTCCGCTGGTGACCCCCACCTCCCAGATCGTAGGCGT
GCAGGCGATGCTGAACGTCAAGTTCGGCCGTTGGAAGAACATCACCCCCCAGGCGGCGGACATTGCCCTGGGCTACTACG
GCCGCACCTCGGCTCCCGTGGACCCGGAAGTGCAGAAACTCTGCGCGGAAAAGATGGGCAAGGACCCGATCACCTGCCGT
CCGGCGGACCTGATCAAGCCCGGCATGGAAGACCTGCGCAAGAAGCTTGCGGAAAAGGGGCTCCCCACGGACGACGAACA
CTGCGTCATCTACGCCATGTTCCCGCAGCAGGTGGAAGACTTCTACAAAAAGCCGGAACCTAAGGAAGAAGCCCCCGTAC
AGGTAAACACCGCTCCTGCCGCAACGCCTGCGTCTGCCGCGCCGTCCATGACCGTCATTGACAACGGAATCACCGCCCGG
GTAAGCGGCCCTTCCGGTTCCCGTGACCTGACCATCATCATCAACGGCCAGGCCCACTCCGTGAAAGTGGAACGCATCGG
CTAA

Upstream 100 bases:

>100_bases
CGGCGAATCCGCTGTTGTATGCTTGACCTGCAAGGGAGGCTGTGAGAAAATCACCCTCCAGAAGCCACCTCTTCAATTTT
TAACAACAGTTAACTAAATA

Downstream 100 bases:

>100_bases
AATCTCCGAAGCATCTGATCAAACCGTTTGCGGGCAGCCCCTCCATAAAGGGACTGCCCGCTTTACTGTTTATTGAGAAA
CGGCGTTTTCGTCACAGCTC

Product: Methylmalonyl-CoA carboxytransferase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNPVTFNCTVLRDGHQSLAATRMKTADMLPIAPILDSMGFSALETWGGATIDAGLRFLKEWPFDRLDALKKAAPKTPHMM
LLRGQNIVGYTNYADDVVEAFVAMSAKHGMNIFRIFDCVNDPRNMETSIRAAKKAGAQAHGTICYTTSPVHTTQTFVDMG
RELADMGADAIVIKDMAGLIPPYVTQELVSALKKDLNIPVWIHTHDTAGLGASTYLSAIDAGVDACDVSISPFANGTGQP
DCLRMLALLNGNPRKPDYDADKLIEVSEMLKPVYEGLGKFASHRNEVVDSDTLRYQVPGGMLSNFRTQLKEQGMEDKFEE
VFAEIPVVRKALGWIPLVTPTSQIVGVQAMLNVKFGRWKNITPQAADIALGYYGRTSAPVDPEVQKLCAEKMGKDPITCR
PADLIKPGMEDLRKKLAEKGLPTDDEHCVIYAMFPQQVEDFYKKPEPKEEAPVQVNTAPAATPASAAPSMTVIDNGITAR
VSGPSGSRDLTIIINGQAHSVKVERIG

Sequences:

>Translated_507_residues
MNPVTFNCTVLRDGHQSLAATRMKTADMLPIAPILDSMGFSALETWGGATIDAGLRFLKEWPFDRLDALKKAAPKTPHMM
LLRGQNIVGYTNYADDVVEAFVAMSAKHGMNIFRIFDCVNDPRNMETSIRAAKKAGAQAHGTICYTTSPVHTTQTFVDMG
RELADMGADAIVIKDMAGLIPPYVTQELVSALKKDLNIPVWIHTHDTAGLGASTYLSAIDAGVDACDVSISPFANGTGQP
DCLRMLALLNGNPRKPDYDADKLIEVSEMLKPVYEGLGKFASHRNEVVDSDTLRYQVPGGMLSNFRTQLKEQGMEDKFEE
VFAEIPVVRKALGWIPLVTPTSQIVGVQAMLNVKFGRWKNITPQAADIALGYYGRTSAPVDPEVQKLCAEKMGKDPITCR
PADLIKPGMEDLRKKLAEKGLPTDDEHCVIYAMFPQQVEDFYKKPEPKEEAPVQVNTAPAATPASAAPSMTVIDNGITAR
VSGPSGSRDLTIIINGQAHSVKVERIG
>Mature_507_residues
MNPVTFNCTVLRDGHQSLAATRMKTADMLPIAPILDSMGFSALETWGGATIDAGLRFLKEWPFDRLDALKKAAPKTPHMM
LLRGQNIVGYTNYADDVVEAFVAMSAKHGMNIFRIFDCVNDPRNMETSIRAAKKAGAQAHGTICYTTSPVHTTQTFVDMG
RELADMGADAIVIKDMAGLIPPYVTQELVSALKKDLNIPVWIHTHDTAGLGASTYLSAIDAGVDACDVSISPFANGTGQP
DCLRMLALLNGNPRKPDYDADKLIEVSEMLKPVYEGLGKFASHRNEVVDSDTLRYQVPGGMLSNFRTQLKEQGMEDKFEE
VFAEIPVVRKALGWIPLVTPTSQIVGVQAMLNVKFGRWKNITPQAADIALGYYGRTSAPVDPEVQKLCAEKMGKDPITCR
PADLIKPGMEDLRKKLAEKGLPTDDEHCVIYAMFPQQVEDFYKKPEPKEEAPVQVNTAPAATPASAAPSMTVIDNGITAR
VSGPSGSRDLTIIINGQAHSVKVERIG

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=361, Percent_Identity=36.5650969529086, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI106049295, Length=361, Percent_Identity=36.5650969529086, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI106049292, Length=361, Percent_Identity=36.5650969529086, Blast_Score=222, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17562816, Length=527, Percent_Identity=29.9810246679317, Blast_Score=222, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6321376, Length=533, Percent_Identity=29.6435272045028, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319695, Length=533, Percent_Identity=29.6435272045028, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI281363050, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652224, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652222, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652220, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652218, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652212, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652210, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652214, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI19921944, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24652216, Length=525, Percent_Identity=31.4285714285714, Blast_Score=231, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 55206; Mature: 55206

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00018 EF_HAND_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVTFNCTVLRDGHQSLAATRMKTADMLPIAPILDSMGFSALETWGGATIDAGLRFLKE
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCHHHHHHHHHH
WPFDRLDALKKAAPKTPHMMLLRGQNIVGYTNYADDVVEAFVAMSAKHGMNIFRIFDCVN
CCHHHHHHHHHHCCCCCCEEEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCEEHHHHHC
DPRNMETSIRAAKKAGAQAHGTICYTTSPVHTTQTFVDMGRELADMGADAIVIKDMAGLI
CCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
PPYVTQELVSALKKDLNIPVWIHTHDTAGLGASTYLSAIDAGVDACDVSISPFANGTGQP
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCEECCCCCCCCCH
DCLRMLALLNGNPRKPDYDADKLIEVSEMLKPVYEGLGKFASHRNEVVDSDTLRYQVPGG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCH
MLSNFRTQLKEQGMEDKFEEVFAEIPVVRKALGWIPLVTPTSQIVGVQAMLNVKFGRWKN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCC
ITPQAADIALGYYGRTSAPVDPEVQKLCAEKMGKDPITCRPADLIKPGMEDLRKKLAEKG
CCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHCC
LPTDDEHCVIYAMFPQQVEDFYKKPEPKEEAPVQVNTAPAATPASAAPSMTVIDNGITAR
CCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCEEEE
VSGPSGSRDLTIIINGQAHSVKVERIG
ECCCCCCCEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MNPVTFNCTVLRDGHQSLAATRMKTADMLPIAPILDSMGFSALETWGGATIDAGLRFLKE
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCHHHHHHHHHH
WPFDRLDALKKAAPKTPHMMLLRGQNIVGYTNYADDVVEAFVAMSAKHGMNIFRIFDCVN
CCHHHHHHHHHHCCCCCCEEEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCEEHHHHHC
DPRNMETSIRAAKKAGAQAHGTICYTTSPVHTTQTFVDMGRELADMGADAIVIKDMAGLI
CCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCC
PPYVTQELVSALKKDLNIPVWIHTHDTAGLGASTYLSAIDAGVDACDVSISPFANGTGQP
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCEECCCCCCCCCH
DCLRMLALLNGNPRKPDYDADKLIEVSEMLKPVYEGLGKFASHRNEVVDSDTLRYQVPGG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCH
MLSNFRTQLKEQGMEDKFEEVFAEIPVVRKALGWIPLVTPTSQIVGVQAMLNVKFGRWKN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCC
ITPQAADIALGYYGRTSAPVDPEVQKLCAEKMGKDPITCRPADLIKPGMEDLRKKLAEKG
CCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHCC
LPTDDEHCVIYAMFPQQVEDFYKKPEPKEEAPVQVNTAPAATPASAAPSMTVIDNGITAR
CCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCEEEE
VSGPSGSRDLTIIINGQAHSVKVERIG
ECCCCCCCEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]