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The map label for this gene is cls [H]

Identifier: 187736512

GI number: 187736512

Start: 2468259

End: 2469713

Strand: Direct

Name: cls [H]

Synonym: Amuc_2033

Alternate gene names: 187736512

Gene position: 2468259-2469713 (Clockwise)

Preceding gene: 187736509

Following gene: 187736513

Centisome position: 92.65

GC content: 57.94

Gene sequence:

>1455_bases
ATGATCATTCCTGAAGAGCCATATTTGGGATTTGCCGCCTTTTGCCACATTGCAGGCGCCTTCTGCCTGATTCCGGCCCT
GCTCCATACGCGCACGCCTCAGGGAACAATCGCGTGGCTCATTTCCCTGCTGGCATTTCCATATATTGCCGTCCCCTTTT
ACCTGATCCTGGGACGCCGGAGATTCAGCGGATATGTGGAAACGCGCCGCCGCCAGACGGACCCCGAATCCGCATGGGGG
GAACTGACGGATAAAATTACAAACTGCATGGTTCCGTATGCCGTACAGTCATCCGACACGGCGGGAAAAATCATGCACAC
GCTTTCCAATATCGTGCGGCTGCCCGTCTGCCGCGGCAATTCCTGCCGCCTGCTTATTGACGCGGCCAACGCTTTCCCGC
GCATCTACGACGCCATCAAGAACGCAGAGCATTACATCCTGATTGAATTCTTCATCATCAAAAACGACTCCGTGGGGCAG
AACCTGAAGGACCTGCTGATTGAACGGGCCAAGGCCGGCATCCGCATTTACATGCTGTATGATGAAATCGGCTCCCACAA
GCTCCCCCCCGGTTACATTTCCGCCCTGCGGAAAGCAGGAGTGAACATTGAACCTTTCAACGGGAAGCGCCATTTCCTGA
GCAACATCCTGCGGCTGAACTTCCGCAACCACCGGAAACTGGTGGTGGTGGACGGTTCCACCGCCTTCATCGGAGGCATG
AATATAGGCAGGGAATACCTGGGCAAGGGAGCTCTGGGCTACTGGCGCGACACGTTTGTCCAGCTTGAAGGCCCCTCCGT
CCAGCAAACGCAAATCAGCTTTCTGGAAGACTGGAACTGGGCCATGCTGAAATGCGGCCCCTCCTCCCTTCCCCGCCTCC
GCTGGGAAATCACGCCCCAGCCGGAGGATGAAACCCTGCTCATCCTGCCCTCCGGCCCCGCAGACGTCATTCCCGCCTGG
AAAACCGCGCTCATCGCCCTGGCGAACAGCGCCACCCGGCGGCTCTGGATATCCACCCCCTACTTCGTCCCGGACGAGGG
AGTCATGGCCGCCCTGCAGGCGGCGGCCCTGCGGAACGTGGATGTGCGCATCCTGCGTCCGGAGCGGGCAGACCATATTC
TGGTGAAACTGTCCTCCTTTACTTTCCTTCGGGACCTGGACACATACGGCATCCAGCTCTGGGCCTATCAAAAGGGGTTC
CTGCATCAGAAAGTAGTTCTGATGGACGATGACATAGCCACCGTGGGAACCGCCAATCTGGACAACCGTTCCCTGGCCCT
GAACTTTGAAATCACCGCCGTTATCCATTCCCCCTCCGCCTGCGCGGAGGTAAAAGCCATGCTGGAGCAGGATTTTTCCT
CCTCCAAGAGGGAATCCCTGGCGGATTACAACAATAAATCCCTGGGCTTCAAAATGCTCTGCAACCTGGCGCGACTGACG
GCCCCCGTCCAGTAG

Upstream 100 bases:

>100_bases
GAGCATGCGTCACCTTAGTATCATCTCCTGCTCCAAAAGCCAGCCTTTTCACTTCTCCCCGCCGTTTTCATCCCCCGCAT
CTTTTCCGGTTTTCCGTTCC

Downstream 100 bases:

>100_bases
TTCTTCCGGCGAATCCGCTGTTGTATGCTTGACCTGCAAGGGAGGCTGTGAGAAAATCACCCTCCAGAAGCCACCTCTTC
AATTTTTAACAACAGTTAAC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MIIPEEPYLGFAAFCHIAGAFCLIPALLHTRTPQGTIAWLISLLAFPYIAVPFYLILGRRRFSGYVETRRRQTDPESAWG
ELTDKITNCMVPYAVQSSDTAGKIMHTLSNIVRLPVCRGNSCRLLIDAANAFPRIYDAIKNAEHYILIEFFIIKNDSVGQ
NLKDLLIERAKAGIRIYMLYDEIGSHKLPPGYISALRKAGVNIEPFNGKRHFLSNILRLNFRNHRKLVVVDGSTAFIGGM
NIGREYLGKGALGYWRDTFVQLEGPSVQQTQISFLEDWNWAMLKCGPSSLPRLRWEITPQPEDETLLILPSGPADVIPAW
KTALIALANSATRRLWISTPYFVPDEGVMAALQAAALRNVDVRILRPERADHILVKLSSFTFLRDLDTYGIQLWAYQKGF
LHQKVVLMDDDIATVGTANLDNRSLALNFEITAVIHSPSACAEVKAMLEQDFSSSKRESLADYNNKSLGFKMLCNLARLT
APVQ

Sequences:

>Translated_484_residues
MIIPEEPYLGFAAFCHIAGAFCLIPALLHTRTPQGTIAWLISLLAFPYIAVPFYLILGRRRFSGYVETRRRQTDPESAWG
ELTDKITNCMVPYAVQSSDTAGKIMHTLSNIVRLPVCRGNSCRLLIDAANAFPRIYDAIKNAEHYILIEFFIIKNDSVGQ
NLKDLLIERAKAGIRIYMLYDEIGSHKLPPGYISALRKAGVNIEPFNGKRHFLSNILRLNFRNHRKLVVVDGSTAFIGGM
NIGREYLGKGALGYWRDTFVQLEGPSVQQTQISFLEDWNWAMLKCGPSSLPRLRWEITPQPEDETLLILPSGPADVIPAW
KTALIALANSATRRLWISTPYFVPDEGVMAALQAAALRNVDVRILRPERADHILVKLSSFTFLRDLDTYGIQLWAYQKGF
LHQKVVLMDDDIATVGTANLDNRSLALNFEITAVIHSPSACAEVKAMLEQDFSSSKRESLADYNNKSLGFKMLCNLARLT
APVQ
>Mature_484_residues
MIIPEEPYLGFAAFCHIAGAFCLIPALLHTRTPQGTIAWLISLLAFPYIAVPFYLILGRRRFSGYVETRRRQTDPESAWG
ELTDKITNCMVPYAVQSSDTAGKIMHTLSNIVRLPVCRGNSCRLLIDAANAFPRIYDAIKNAEHYILIEFFIIKNDSVGQ
NLKDLLIERAKAGIRIYMLYDEIGSHKLPPGYISALRKAGVNIEPFNGKRHFLSNILRLNFRNHRKLVVVDGSTAFIGGM
NIGREYLGKGALGYWRDTFVQLEGPSVQQTQISFLEDWNWAMLKCGPSSLPRLRWEITPQPEDETLLILPSGPADVIPAW
KTALIALANSATRRLWISTPYFVPDEGVMAALQAAALRNVDVRILRPERADHILVKLSSFTFLRDLDTYGIQLWAYQKGF
LHQKVVLMDDDIATVGTANLDNRSLALNFEITAVIHSPSACAEVKAMLEQDFSSSKRESLADYNNKSLGFKMLCNLARLT
APVQ

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=440, Percent_Identity=28.6363636363636, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787007, Length=329, Percent_Identity=30.3951367781155, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI87081826, Length=180, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54290; Mature: 54290

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPEEPYLGFAAFCHIAGAFCLIPALLHTRTPQGTIAWLISLLAFPYIAVPFYLILGRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
RFSGYVETRRRQTDPESAWGELTDKITNCMVPYAVQSSDTAGKIMHTLSNIVRLPVCRGN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCC
SCRLLIDAANAFPRIYDAIKNAEHYILIEFFIIKNDSVGQNLKDLLIERAKAGIRIYMLY
CCEEEEECCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEE
DEIGSHKLPPGYISALRKAGVNIEPFNGKRHFLSNILRLNFRNHRKLVVVDGSTAFIGGM
ECCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEEECC
NIGREYLGKGALGYWRDTFVQLEGPSVQQTQISFLEDWNWAMLKCGPSSLPRLRWEITPQ
HHCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEECCC
PEDETLLILPSGPADVIPAWKTALIALANSATRRLWISTPYFVPDEGVMAALQAAALRNV
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
DVRILRPERADHILVKLSSFTFLRDLDTYGIQLWAYQKGFLHQKVVLMDDDIATVGTANL
CEEEECCCCCCEEEEEEECHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCEEECCCC
DNRSLALNFEITAVIHSPSACAEVKAMLEQDFSSSKRESLADYNNKSLGFKMLCNLARLT
CCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHC
APVQ
CCCC
>Mature Secondary Structure
MIIPEEPYLGFAAFCHIAGAFCLIPALLHTRTPQGTIAWLISLLAFPYIAVPFYLILGRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
RFSGYVETRRRQTDPESAWGELTDKITNCMVPYAVQSSDTAGKIMHTLSNIVRLPVCRGN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCC
SCRLLIDAANAFPRIYDAIKNAEHYILIEFFIIKNDSVGQNLKDLLIERAKAGIRIYMLY
CCEEEEECCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEE
DEIGSHKLPPGYISALRKAGVNIEPFNGKRHFLSNILRLNFRNHRKLVVVDGSTAFIGGM
ECCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEEECC
NIGREYLGKGALGYWRDTFVQLEGPSVQQTQISFLEDWNWAMLKCGPSSLPRLRWEITPQ
HHCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEECCC
PEDETLLILPSGPADVIPAWKTALIALANSATRRLWISTPYFVPDEGVMAALQAAALRNV
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
DVRILRPERADHILVKLSSFTFLRDLDTYGIQLWAYQKGFLHQKVVLMDDDIATVGTANL
CEEEECCCCCCEEEEEEECHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCEEECCCC
DNRSLALNFEITAVIHSPSACAEVKAMLEQDFSSSKRESLADYNNKSLGFKMLCNLARLT
CCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHC
APVQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]