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The map label for this gene is thyA [H]

Identifier: 187736511

GI number: 187736511

Start: 2467299

End: 2468114

Strand: Reverse

Name: thyA [H]

Synonym: Amuc_2032

Alternate gene names: 187736511

Gene position: 2468114-2467299 (Counterclockwise)

Preceding gene: 187736514

Following gene: 187736510

Centisome position: 92.64

GC content: 55.88

Gene sequence:

>816_bases
ATGAAGCAGTATTTGGCACTTTTGGAGGATGTCCTGAGCAATGGAGCGGGGCGGGAGGACCGTACCGGAACGGGCACTAT
CGGCGTGTTCGGGCGGCAGAGCAGGTATGATTTGCGCGACGGTTTTCCATGCCTTACCACCAAGAAGCTTCATTTACGCT
CCATCATTTATGAGCTGCTCTGGTTCCTGAAAGGAGAGACCAATATCAAGTTTTTGAAAGACAACGGCGTCAGCATCTGG
GATGAATGGGCCGACGAAAACGGGGATCTGGGGCCCGTGTACGGCGCGCAGTGGCGCTGCTGGCCGGGAAACGACGGCAA
ACCCATTGACCAGATTGCCAGACTGGTTGACGGCCTGAAACACAATCCATGGTCTCGGAGGCACATCGTCAGTGCGTGGA
ATGTGGCCCTGGTGGATGACATGGCCCTCCCTCCGTGCCATTCCCTGTTCCAGTTTTGCGTGATTCCCGCCCAGCCGGGA
GAAAAAAAGCACGGCCTTTCCCTCCAGCTTTACCAGCGCAGCGCGGATCTTTTTCTGGGGGTTCCCTTCAACATTGCTTC
CTATGCGCTGCTGTTGCTTATGACCGCCCAGGTATGCGGGTATGAAGCCCGGGAATTCATCCATACGTTCGGAGATCTGC
ACCTGTACCGGAACCATCTGGACCAGGCGAGGGAACAACTCTCCCGCACGCCCCGTCCTCTCCCGGTCATGAAGCTCAAT
CCGGAGGTAAAAACCATTGACGGATTCCATTATGAAGATTTTGAGCTCGTGGGGTATGATCCCCTGCCGCACATCAAGGC
TCCCATCTCCGTTTAG

Upstream 100 bases:

>100_bases
AGAAGTGAAAAGGCTGGCTTTTGGAGCAGGAGATGATACTAAGGTGACGCATGCTCGTATTATCACGGGTCCAGGGATTA
GAAAAGCGTAATTTAAGGGA

Downstream 100 bases:

>100_bases
AACATTCATCCCTTAATCCATCATGTCACAACCCGTTACTTATACAGGAGTGGTCGCCATGGCTCCCGACCGCGGCATTG
GTTACCGGGGGGCGCTCCCC

Product: Thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKQYLALLEDVLSNGAGREDRTGTGTIGVFGRQSRYDLRDGFPCLTTKKLHLRSIIYELLWFLKGETNIKFLKDNGVSIW
DEWADENGDLGPVYGAQWRCWPGNDGKPIDQIARLVDGLKHNPWSRRHIVSAWNVALVDDMALPPCHSLFQFCVIPAQPG
EKKHGLSLQLYQRSADLFLGVPFNIASYALLLLMTAQVCGYEAREFIHTFGDLHLYRNHLDQAREQLSRTPRPLPVMKLN
PEVKTIDGFHYEDFELVGYDPLPHIKAPISV

Sequences:

>Translated_271_residues
MKQYLALLEDVLSNGAGREDRTGTGTIGVFGRQSRYDLRDGFPCLTTKKLHLRSIIYELLWFLKGETNIKFLKDNGVSIW
DEWADENGDLGPVYGAQWRCWPGNDGKPIDQIARLVDGLKHNPWSRRHIVSAWNVALVDDMALPPCHSLFQFCVIPAQPG
EKKHGLSLQLYQRSADLFLGVPFNIASYALLLLMTAQVCGYEAREFIHTFGDLHLYRNHLDQAREQLSRTPRPLPVMKLN
PEVKTIDGFHYEDFELVGYDPLPHIKAPISV
>Mature_271_residues
MKQYLALLEDVLSNGAGREDRTGTGTIGVFGRQSRYDLRDGFPCLTTKKLHLRSIIYELLWFLKGETNIKFLKDNGVSIW
DEWADENGDLGPVYGAQWRCWPGNDGKPIDQIARLVDGLKHNPWSRRHIVSAWNVALVDDMALPPCHSLFQFCVIPAQPG
EKKHGLSLQLYQRSADLFLGVPFNIASYALLLLMTAQVCGYEAREFIHTFGDLHLYRNHLDQAREQLSRTPRPLPVMKLN
PEVKTIDGFHYEDFELVGYDPLPHIKAPISV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=47.0588235294118, Blast_Score=259, Evalue=1e-69,
Organism=Escherichia coli, GI1789191, Length=271, Percent_Identity=59.0405904059041, Blast_Score=334, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI71993377, Length=290, Percent_Identity=43.448275862069, Blast_Score=230, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324648, Length=227, Percent_Identity=48.0176211453744, Blast_Score=196, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17137556, Length=288, Percent_Identity=47.9166666666667, Blast_Score=265, Evalue=2e-71,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30844; Mature: 30844

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLALLEDVLSNGAGREDRTGTGTIGVFGRQSRYDLRDGFPCLTTKKLHLRSIIYELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHH
WFLKGETNIKFLKDNGVSIWDEWADENGDLGPVYGAQWRCWPGNDGKPIDQIARLVDGLK
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHC
HNPWSRRHIVSAWNVALVDDMALPPCHSLFQFCVIPAQPGEKKHGLSLQLYQRSADLFLG
CCCCCHHHHHHHHCEEEECCCCCCCHHHHHHHEECCCCCCCCCCCCEEEEEECCCCEEEE
VPFNIASYALLLLMTAQVCGYEAREFIHTFGDLHLYRNHLDQAREQLSRTPRPLPVMKLN
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PEVKTIDGFHYEDFELVGYDPLPHIKAPISV
CCEEEECCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLALLEDVLSNGAGREDRTGTGTIGVFGRQSRYDLRDGFPCLTTKKLHLRSIIYELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHH
WFLKGETNIKFLKDNGVSIWDEWADENGDLGPVYGAQWRCWPGNDGKPIDQIARLVDGLK
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHC
HNPWSRRHIVSAWNVALVDDMALPPCHSLFQFCVIPAQPGEKKHGLSLQLYQRSADLFLG
CCCCCHHHHHHHHCEEEECCCCCCCHHHHHHHEECCCCCCCCCCCCEEEEEECCCCEEEE
VPFNIASYALLLLMTAQVCGYEAREFIHTFGDLHLYRNHLDQAREQLSRTPRPLPVMKLN
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PEVKTIDGFHYEDFELVGYDPLPHIKAPISV
CCEEEECCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9228757 [H]