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The map label for this gene is folA [H]

Identifier: 187736510

GI number: 187736510

Start: 2466797

End: 2467276

Strand: Reverse

Name: folA [H]

Synonym: Amuc_2031

Alternate gene names: 187736510

Gene position: 2467276-2466797 (Counterclockwise)

Preceding gene: 187736511

Following gene: 187736506

Centisome position: 92.61

GC content: 56.67

Gene sequence:

>480_bases
ATGTCACAACCCGTTACTTATACAGGAGTGGTCGCCATGGCTCCCGACCGCGGCATTGGTTACCGGGGGGCGCTCCCCTG
GCATTTGCCGGACGACCTCAAGACGTTCAAGCGCATTACGACGGGGCACCCCGTGCTGATGGGCCGGAAAACGTATGAGA
GCATAGGCAGGCCTCTTCCCGGCAGGCAGAACATCGTGCTGACCCGTGACCCGGCGTGGACGGCGGAAGGCGTTCAGGTG
GTTCATTCCGCAGGGGAGTTGGAATGTCTGGAATTGATGGACCCGGAAATCATGGTGATCGGGGGTGCGGAGATTTTTTC
CCTGATGATGCCGGCCATGTCCCGCATGTGGGTTTCCAAAGTAAAGGGGGAATATCCGGCGGATACTTTTCTTCCTCCGT
TTGAGAACCGGCTGGAGCGTGCCGTCCTGAAGGAACATTTTGAAGATTTTGACCTGTATTTGTACGAGCAGGTCAAATAG

Upstream 100 bases:

>100_bases
GACGGATTCCATTATGAAGATTTTGAGCTCGTGGGGTATGATCCCCTGCCGCACATCAAGGCTCCCATCTCCGTTTAGAA
CATTCATCCCTTAATCCATC

Downstream 100 bases:

>100_bases
CCGCTCTTGTCCGTAACTCCGCGGGAGGCGCTGAAGCATAAAAAAGAAAAAGACGGGGCGGCCAAGGCCGCCCCGTCCTT
CATGGAGCAGTCTTCCTTTA

Product: Dihydrofolate reductase

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSQPVTYTGVVAMAPDRGIGYRGALPWHLPDDLKTFKRITTGHPVLMGRKTYESIGRPLPGRQNIVLTRDPAWTAEGVQV
VHSAGELECLELMDPEIMVIGGAEIFSLMMPAMSRMWVSKVKGEYPADTFLPPFENRLERAVLKEHFEDFDLYLYEQVK

Sequences:

>Translated_159_residues
MSQPVTYTGVVAMAPDRGIGYRGALPWHLPDDLKTFKRITTGHPVLMGRKTYESIGRPLPGRQNIVLTRDPAWTAEGVQV
VHSAGELECLELMDPEIMVIGGAEIFSLMMPAMSRMWVSKVKGEYPADTFLPPFENRLERAVLKEHFEDFDLYLYEQVK
>Mature_158_residues
SQPVTYTGVVAMAPDRGIGYRGALPWHLPDDLKTFKRITTGHPVLMGRKTYESIGRPLPGRQNIVLTRDPAWTAEGVQVV
HSAGELECLELMDPEIMVIGGAEIFSLMMPAMSRMWVSKVKGEYPADTFLPPFENRLERAVLKEHFEDFDLYLYEQVK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=142, Percent_Identity=42.9577464788732, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17505899, Length=146, Percent_Identity=34.9315068493151, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324810, Length=149, Percent_Identity=36.241610738255, Blast_Score=74, Evalue=7e-15,
Organism=Drosophila melanogaster, GI116008026, Length=143, Percent_Identity=34.2657342657343, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647458, Length=143, Percent_Identity=34.2657342657343, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17996; Mature: 17865

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPVTYTGVVAMAPDRGIGYRGALPWHLPDDLKTFKRITTGHPVLMGRKTYESIGRPLP
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHCCCCC
GRQNIVLTRDPAWTAEGVQVVHSAGELECLELMDPEIMVIGGAEIFSLMMPAMSRMWVSK
CCCCEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHH
VKGEYPADTFLPPFENRLERAVLKEHFEDFDLYLYEQVK
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
SQPVTYTGVVAMAPDRGIGYRGALPWHLPDDLKTFKRITTGHPVLMGRKTYESIGRPLP
CCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHCCCCC
GRQNIVLTRDPAWTAEGVQVVHSAGELECLELMDPEIMVIGGAEIFSLMMPAMSRMWVSK
CCCCEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHH
VKGEYPADTFLPPFENRLERAVLKEHFEDFDLYLYEQVK
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA