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The map label for this gene is pyrC [H]

Identifier: 187736506

GI number: 187736506

Start: 2460342

End: 2461343

Strand: Reverse

Name: pyrC [H]

Synonym: Amuc_2027

Alternate gene names: 187736506

Gene position: 2461343-2460342 (Counterclockwise)

Preceding gene: 187736510

Following gene: 187736505

Centisome position: 92.39

GC content: 59.58

Gene sequence:

>1002_bases
ATGATTCTGGAACTGCACTCCCCGCTGGACATGCATCTTCACCTGAGGGACGGCGATATGATGAAGCTGGTCGCTCCGTT
AAGTTCCGCCTCTTTTGCCGGGGCGGTCATCATGCCCAACCTGGTGCCTCCGGTGGCTGATGCGGGTGCGGTGCAGGCTT
ACCGGCAGCGGGTGCTGGACGCTTGCGGAGACGATGTATTCCAGCCGTACATGACGGCATTTTTTCGCTCCTATTCAGAA
AAGGAATTGTCCCGGCTCAAGGAACTGGTGTTCGGCATCAAGCTGTACCCGGCAGGAGCCACCACGAACAGCGAGGGCGG
CGTGAAGGCCATGAAGGATGCGGAAGCTACCCTGTCCATCATGCAGGAAATGGATATTCCTTTGCTGGTGCATGGCGAAA
GCCACGGCTTCGTGATGGACCGGGAGGCCGAATTCCTGGATGTTTACCGTGATTTGGCTACGCGCTTCCCCCGGCTGACT
ATCTGCATGGAACATATTACCACGGCCGCCGCCGTGCAGCTGCTGGACGAATTTGAAAACCTGGCCGCCACGGTAACCCT
CCAGCATCTTCTCATTACTTTGGACGATGTGGCCGGTGGCATGCTGAGGCCGCATCTGTTCTGCAAGCCGATCGCCAAAA
GGCCGGAAGACCGGGAAGCCCTGTTGCAGGCTGCCCTTTCCGGGCATCCCCGCCTCATGTTCGGCAGTGACTCCGCCCCC
CATCCCATCCATGCCAAGGAAGCGTGCGGATGCGCCGCCGGCGTGTTTACCGCCCCCATCGCTCTTCCTCGTCTGGCGGC
CCTGTTTGACGAACACGGGGCCCTGGACCGGTTGCAGGGCTTTGTTTCCGGTCATGCCTGCGCTTTGTACGGGTTGAATC
CGCCTGCCAGGACGGTCCGTCTGCAGCGGCGTGAAATGCTGGTGCCGGACGCTTATGAAGGACATGGACAGAAAGTGGTG
CCGATGGATGCCGGATGCACCATTCCTTGGAGACTGATATGA

Upstream 100 bases:

>100_bases
GCGGGAAAATGCCCGTTTTCCCTGCCCGTTGGAGAATTGCTTTCCTGCAGGGCGGGGGAGCGTGCTTGATTTAGGCGCAA
TCTTCCCTCATGATGTCGGC

Downstream 100 bases:

>100_bases
GGGGCTTTTCCAGGGGAAGATGACAGAAAGCTATTTAAGGGAACCGTCTTTATACTGGATATTCCGGGCAATCTCACTAC
AATCCCTGCCCATGGATCGG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MILELHSPLDMHLHLRDGDMMKLVAPLSSASFAGAVIMPNLVPPVADAGAVQAYRQRVLDACGDDVFQPYMTAFFRSYSE
KELSRLKELVFGIKLYPAGATTNSEGGVKAMKDAEATLSIMQEMDIPLLVHGESHGFVMDREAEFLDVYRDLATRFPRLT
ICMEHITTAAAVQLLDEFENLAATVTLQHLLITLDDVAGGMLRPHLFCKPIAKRPEDREALLQAALSGHPRLMFGSDSAP
HPIHAKEACGCAAGVFTAPIALPRLAALFDEHGALDRLQGFVSGHACALYGLNPPARTVRLQRREMLVPDAYEGHGQKVV
PMDAGCTIPWRLI

Sequences:

>Translated_333_residues
MILELHSPLDMHLHLRDGDMMKLVAPLSSASFAGAVIMPNLVPPVADAGAVQAYRQRVLDACGDDVFQPYMTAFFRSYSE
KELSRLKELVFGIKLYPAGATTNSEGGVKAMKDAEATLSIMQEMDIPLLVHGESHGFVMDREAEFLDVYRDLATRFPRLT
ICMEHITTAAAVQLLDEFENLAATVTLQHLLITLDDVAGGMLRPHLFCKPIAKRPEDREALLQAALSGHPRLMFGSDSAP
HPIHAKEACGCAAGVFTAPIALPRLAALFDEHGALDRLQGFVSGHACALYGLNPPARTVRLQRREMLVPDAYEGHGQKVV
PMDAGCTIPWRLI
>Mature_333_residues
MILELHSPLDMHLHLRDGDMMKLVAPLSSASFAGAVIMPNLVPPVADAGAVQAYRQRVLDACGDDVFQPYMTAFFRSYSE
KELSRLKELVFGIKLYPAGATTNSEGGVKAMKDAEATLSIMQEMDIPLLVHGESHGFVMDREAEFLDVYRDLATRFPRLT
ICMEHITTAAAVQLLDEFENLAATVTLQHLLITLDDVAGGMLRPHLFCKPIAKRPEDREALLQAALSGHPRLMFGSDSAP
HPIHAKEACGCAAGVFTAPIALPRLAALFDEHGALDRLQGFVSGHACALYGLNPPARTVRLQRREMLVPDAYEGHGQKVV
PMDAGCTIPWRLI

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=342, Percent_Identity=43.2748538011696, Blast_Score=232, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6323452, Length=358, Percent_Identity=34.0782122905028, Blast_Score=192, Evalue=9e-50,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006992
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF04909 Amidohydro_2 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 36405; Mature: 36405

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILELHSPLDMHLHLRDGDMMKLVAPLSSASFAGAVIMPNLVPPVADAGAVQAYRQRVLD
CEEEECCCCEEEEEECCCCCEEHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHH
ACGDDVFQPYMTAFFRSYSEKELSRLKELVFGIKLYPAGATTNSEGGVKAMKDAEATLSI
HHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHH
MQEMDIPLLVHGESHGFVMDREAEFLDVYRDLATRFPRLTICMEHITTAAAVQLLDEFEN
HHHCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAATVTLQHLLITLDDVAGGMLRPHLFCKPIAKRPEDREALLQAALSGHPRLMFGSDSAP
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCC
HPIHAKEACGCAAGVFTAPIALPRLAALFDEHGALDRLQGFVSGHACALYGLNPPARTVR
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCHHHHH
LQRREMLVPDAYEGHGQKVVPMDAGCTIPWRLI
HHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEC
>Mature Secondary Structure
MILELHSPLDMHLHLRDGDMMKLVAPLSSASFAGAVIMPNLVPPVADAGAVQAYRQRVLD
CEEEECCCCEEEEEECCCCCEEHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHH
ACGDDVFQPYMTAFFRSYSEKELSRLKELVFGIKLYPAGATTNSEGGVKAMKDAEATLSI
HHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHH
MQEMDIPLLVHGESHGFVMDREAEFLDVYRDLATRFPRLTICMEHITTAAAVQLLDEFEN
HHHCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAATVTLQHLLITLDDVAGGMLRPHLFCKPIAKRPEDREALLQAALSGHPRLMFGSDSAP
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCC
HPIHAKEACGCAAGVFTAPIALPRLAALFDEHGALDRLQGFVSGHACALYGLNPPARTVR
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCHHHHH
LQRREMLVPDAYEGHGQKVVPMDAGCTIPWRLI
HHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]