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The map label for this gene is mdeA [H]

Identifier: 187736492

GI number: 187736492

Start: 2445725

End: 2447011

Strand: Direct

Name: mdeA [H]

Synonym: Amuc_2013

Alternate gene names: 187736492

Gene position: 2445725-2447011 (Clockwise)

Preceding gene: 187736491

Following gene: 187736493

Centisome position: 91.8

GC content: 58.9

Gene sequence:

>1287_bases
ATGAGTAAGAACCATCGATTTGAAACACGCCAGATCCATGTGGGTCAGGAAAGTCCGGATCCGGCCACCGATGCGCGCGC
CGTGCCTATTTACGCCACCACTTCGTATGTCTTCAAAGACTCGGAACAGGCGGCAGGCCGTTTTGCCCTGGCGGAGCCGG
GCAATATTTATAACCGCCTGATGAACCCTACCGCAGATGTTTTTGAAAAACGCATCGCCTCCCTGGAAGGAGGGACGGCC
GCGCTGGCCGTCTCCACCGGGGCTGCCGCTGTCACGTATGCCATCCAGAACATCGCCCGGGCCGGGGACCACATCGTTTC
TTCTTCCACCGTATATGGCGGGACGTATAATCTCTTTGCCAATACGCTGGCGGACGCCGGCATAGAAACCACTTTCGTGG
ATGCAAGGGACGTTCAGAATTTTTCCAGGGCCATCCGGAACAATACCAAGGCCCTGTACGTGGAAAGCCTGGGCAACCCG
AACTGCGACATCGTGGATATGGAAGCGCTGGCGGAAGTGGCGCACGCCCACGGCATCCCGCTCATTGTGGACAGCACGTT
CGCCACGCCCTTCCTGTTCCGCCCCCTGGAACACGGAGCGGACATCGTGGTGCATTCCGCTACCAAATTCATCGGCGGCC
ACGGCACGGTGATGGGCGGCGTGATTGTGGACGGCGGTAAATTCGACTGGACGCAGAACGACAAGTTCCCCGGCATCAGC
AAGCCCAACCCCAATTACCACGGAGCCGTGTTCGCTGAGGTATGCGGCAATCTGGCCTATATCGTCAAAATCCGGGCCAC
CCTGCTGCGGGATACGGGAGCCACCATCAGCCCGTTCAACTCCTTCCTGCTGCTCCAGGGGCTGGAAACACTCTCCCTGC
GGGTGGAACGCCATGTGCAGAACGCCCTGCGCGTAGCGGACTATCTGGCCTCCCATCCCCAGGTGGAGAGGGTGAACCAT
CCCTCCCTGCCGGACCATCCGGACCACGACCTTTACAAGAGATACTACCCGAACGGGGGCGGCTCCATCTTCACCTTTGA
AATCAAGGGTGGCGCGGAAAAAGCTCGCAAATTCTGCGAAAGCCTGGAACTATTCTCCCTGCTCGCGAACGTGGCGGACG
TCAAGTCCCTGGTGATTCATCCGGCCTCCACCACCCATTCCCAGATGACGGAGGAGGAACTGAAGGCGGGAGGCATTACG
CCATCCACCGTGCGGCTTTCCATCGGGACGGAACATATCGACGATATTCTGGAAGATCTGGAACAAGGCTTCCGCGCCAT
TCTCTAA

Upstream 100 bases:

>100_bases
TCCCATTCCGGCCCATGTCCCGTTTTTTTTACCCGGAGCTTGACATTTGCTTTCCTTTTCATTATTTATTCCCTAGTTTT
CCGATAGGAATATAAAAACC

Downstream 100 bases:

>100_bases
CGATTTAACGGCAGCAACGCTCTTTTCCTACAAGCCATGAGAATTTACAAAAACATTACGGAACTGATCGGCGGCACCCC
GTTGCTGGAACTGACCAATT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSKNHRFETRQIHVGQESPDPATDARAVPIYATTSYVFKDSEQAAGRFALAEPGNIYNRLMNPTADVFEKRIASLEGGTA
ALAVSTGAAAVTYAIQNIARAGDHIVSSSTVYGGTYNLFANTLADAGIETTFVDARDVQNFSRAIRNNTKALYVESLGNP
NCDIVDMEALAEVAHAHGIPLIVDSTFATPFLFRPLEHGADIVVHSATKFIGGHGTVMGGVIVDGGKFDWTQNDKFPGIS
KPNPNYHGAVFAEVCGNLAYIVKIRATLLRDTGATISPFNSFLLLQGLETLSLRVERHVQNALRVADYLASHPQVERVNH
PSLPDHPDHDLYKRYYPNGGGSIFTFEIKGGAEKARKFCESLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGIT
PSTVRLSIGTEHIDDILEDLEQGFRAIL

Sequences:

>Translated_428_residues
MSKNHRFETRQIHVGQESPDPATDARAVPIYATTSYVFKDSEQAAGRFALAEPGNIYNRLMNPTADVFEKRIASLEGGTA
ALAVSTGAAAVTYAIQNIARAGDHIVSSSTVYGGTYNLFANTLADAGIETTFVDARDVQNFSRAIRNNTKALYVESLGNP
NCDIVDMEALAEVAHAHGIPLIVDSTFATPFLFRPLEHGADIVVHSATKFIGGHGTVMGGVIVDGGKFDWTQNDKFPGIS
KPNPNYHGAVFAEVCGNLAYIVKIRATLLRDTGATISPFNSFLLLQGLETLSLRVERHVQNALRVADYLASHPQVERVNH
PSLPDHPDHDLYKRYYPNGGGSIFTFEIKGGAEKARKFCESLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGIT
PSTVRLSIGTEHIDDILEDLEQGFRAIL
>Mature_427_residues
SKNHRFETRQIHVGQESPDPATDARAVPIYATTSYVFKDSEQAAGRFALAEPGNIYNRLMNPTADVFEKRIASLEGGTAA
LAVSTGAAAVTYAIQNIARAGDHIVSSSTVYGGTYNLFANTLADAGIETTFVDARDVQNFSRAIRNNTKALYVESLGNPN
CDIVDMEALAEVAHAHGIPLIVDSTFATPFLFRPLEHGADIVVHSATKFIGGHGTVMGGVIVDGGKFDWTQNDKFPGISK
PNPNYHGAVFAEVCGNLAYIVKIRATLLRDTGATISPFNSFLLLQGLETLSLRVERHVQNALRVADYLASHPQVERVNHP
SLPDHPDHDLYKRYYPNGGGSIFTFEIKGGAEKARKFCESLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGITP
STVRLSIGTEHIDDILEDLEQGFRAIL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=34.8341232227488, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=31.2796208530806, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=31.1163895486936, Blast_Score=171, Evalue=8e-43,
Organism=Escherichia coli, GI1790375, Length=400, Percent_Identity=34, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=438, Percent_Identity=26.2557077625571, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71980708, Length=423, Percent_Identity=33.096926713948, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17538055, Length=410, Percent_Identity=35.8536585365854, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17560056, Length=408, Percent_Identity=34.3137254901961, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=49.4252873563218, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319307, Length=431, Percent_Identity=32.0185614849188, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321170, Length=365, Percent_Identity=29.5890410958904, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321254, Length=439, Percent_Identity=25.7403189066059, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=436, Percent_Identity=25.2293577981651, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=35.6973995271868, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46408; Mature: 46276

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00589 PTS_HPR_SER ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNHRFETRQIHVGQESPDPATDARAVPIYATTSYVFKDSEQAAGRFALAEPGNIYNRL
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCHHHCCEEEECCCHHHHHHH
MNPTADVFEKRIASLEGGTAALAVSTGAAAVTYAIQNIARAGDHIVSSSTVYGGTYNLFA
CCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHH
NTLADAGIETTFVDARDVQNFSRAIRNNTKALYVESLGNPNCDIVDMEALAEVAHAHGIP
HHHHHCCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHHHHHHHCCCC
LIVDSTFATPFLFRPLEHGADIVVHSATKFIGGHGTVMGGVIVDGGKFDWTQNDKFPGIS
EEEECCCCCHHHHHHHHCCCCEEEECHHHHHCCCCEEEEEEEEECCEECCCCCCCCCCCC
KPNPNYHGAVFAEVCGNLAYIVKIRATLLRDTGATISPFNSFLLLQGLETLSLRVERHVQ
CCCCCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NALRVADYLASHPQVERVNHPSLPDHPDHDLYKRYYPNGGGSIFTFEIKGGAEKARKFCE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHH
SLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGITPSTVRLSIGTEHIDDILEDL
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
EQGFRAIL
HHHHHHCC
>Mature Secondary Structure 
SKNHRFETRQIHVGQESPDPATDARAVPIYATTSYVFKDSEQAAGRFALAEPGNIYNRL
CCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCHHHCCEEEECCCHHHHHHH
MNPTADVFEKRIASLEGGTAALAVSTGAAAVTYAIQNIARAGDHIVSSSTVYGGTYNLFA
CCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHH
NTLADAGIETTFVDARDVQNFSRAIRNNTKALYVESLGNPNCDIVDMEALAEVAHAHGIP
HHHHHCCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHHHHHHHCCCC
LIVDSTFATPFLFRPLEHGADIVVHSATKFIGGHGTVMGGVIVDGGKFDWTQNDKFPGIS
EEEECCCCCHHHHHHHHCCCCEEEECHHHHHCCCCEEEEEEEEECCEECCCCCCCCCCCC
KPNPNYHGAVFAEVCGNLAYIVKIRATLLRDTGATISPFNSFLLLQGLETLSLRVERHVQ
CCCCCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NALRVADYLASHPQVERVNHPSLPDHPDHDLYKRYYPNGGGSIFTFEIKGGAEKARKFCE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHH
SLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGITPSTVRLSIGTEHIDDILEDL
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
EQGFRAIL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]