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The map label for this gene is tag [H]

Identifier: 187736471

GI number: 187736471

Start: 2418686

End: 2419348

Strand: Reverse

Name: tag [H]

Synonym: Amuc_1991

Alternate gene names: 187736471

Gene position: 2419348-2418686 (Counterclockwise)

Preceding gene: 187736472

Following gene: 187736470

Centisome position: 90.81

GC content: 50.68

Gene sequence:

>663_bases
ATGAAGGATATCATCAATGCGCGTTGCGGCTGGGCCGGAACCGATGAACTTTACATAAAATACCATGATGAAGAATGGGG
CAGGCCTGTTTCCGATGACAAAACCCTATTTGAATTTCTGGTGCTGGAGAGCGCCCAGGCGGGATTATCCTGGCTTACTA
TCCTCCGGAAGCGCGAAGGATACCGGAGAGCCTTCCATGGCTTTGATGTGGAGAAAGTGGCGCGGATGACGGCAGAAGAT
GTGGAACGGTTGATGCGATTTGATGGAATTGTCAAAAACCGTTTGAAAATCAACAGCGCTGTCAATAATGCGAAACTGTT
TATGGCCGTTCAGGAAGAATTCGGCAGTTTTTACCGGTACGTCTTGTCGTTTTTCCCCAATCATCAGCCCGTTGTTAACC
ATTTCAGCGCTCTGGCTCAGATTCCCGCTGTTTCTCCGGAGTCGGAAGCCATGAGCAGGGACATGAAAAGGCGGGGATTC
AGGTTTTTCGGTCCGACGATCTGTTATGCGTTTTTCCAGGCAACCGGGTTTGTCAACGACCATATTGAGGGCTGTTTCTG
CAACGCGGCCCAGTACGCAAAATCCCAACCGCAGGTCCAGCCAGAGACGTTGATAACAGGGCCGGAGGGAACAAACGCGG
AAATGACGCATTCTCGTCCATAA

Upstream 100 bases:

>100_bases
TGTTCGGAACCCTGGCCCTGATTACGCTGGGATACTTCGTCTTTCACATGACGCTGGCGGCGTGGCTGTTCAGGAAAAAG
GAATTCTAAGGCGCAACCGT

Downstream 100 bases:

>100_bases
CCATGGGCCTTAATTGGCATTTCCATTTTCTATAAATCGCTTATATGTTGACTGTAAAAAATTATCAACCATGAAAACAG
GTATCATCGGACTTGGAAAA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKDIINARCGWAGTDELYIKYHDEEWGRPVSDDKTLFEFLVLESAQAGLSWLTILRKREGYRRAFHGFDVEKVARMTAED
VERLMRFDGIVKNRLKINSAVNNAKLFMAVQEEFGSFYRYVLSFFPNHQPVVNHFSALAQIPAVSPESEAMSRDMKRRGF
RFFGPTICYAFFQATGFVNDHIEGCFCNAAQYAKSQPQVQPETLITGPEGTNAEMTHSRP

Sequences:

>Translated_220_residues
MKDIINARCGWAGTDELYIKYHDEEWGRPVSDDKTLFEFLVLESAQAGLSWLTILRKREGYRRAFHGFDVEKVARMTAED
VERLMRFDGIVKNRLKINSAVNNAKLFMAVQEEFGSFYRYVLSFFPNHQPVVNHFSALAQIPAVSPESEAMSRDMKRRGF
RFFGPTICYAFFQATGFVNDHIEGCFCNAAQYAKSQPQVQPETLITGPEGTNAEMTHSRP
>Mature_220_residues
MKDIINARCGWAGTDELYIKYHDEEWGRPVSDDKTLFEFLVLESAQAGLSWLTILRKREGYRRAFHGFDVEKVARMTAED
VERLMRFDGIVKNRLKINSAVNNAKLFMAVQEEFGSFYRYVLSFFPNHQPVVNHFSALAQIPAVSPESEAMSRDMKRRGF
RFFGPTICYAFFQATGFVNDHIEGCFCNAAQYAKSQPQVQPETLITGPEGTNAEMTHSRP

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=180, Percent_Identity=51.1111111111111, Blast_Score=205, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 25181; Mature: 25181

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIINARCGWAGTDELYIKYHDEEWGRPVSDDKTLFEFLVLESAQAGLSWLTILRKREG
CCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRRAFHGFDVEKVARMTAEDVERLMRFDGIVKNRLKINSAVNNAKLFMAVQEEFGSFYRY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VLSFFPNHQPVVNHFSALAQIPAVSPESEAMSRDMKRRGFRFFGPTICYAFFQATGFVND
HHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
HIEGCFCNAAQYAKSQPQVQPETLITGPEGTNAEMTHSRP
CCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKDIINARCGWAGTDELYIKYHDEEWGRPVSDDKTLFEFLVLESAQAGLSWLTILRKREG
CCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRRAFHGFDVEKVARMTAEDVERLMRFDGIVKNRLKINSAVNNAKLFMAVQEEFGSFYRY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VLSFFPNHQPVVNHFSALAQIPAVSPESEAMSRDMKRRGFRFFGPTICYAFFQATGFVND
HHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
HIEGCFCNAAQYAKSQPQVQPETLITGPEGTNAEMTHSRP
CCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]