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The map label for this gene is nanA1 [H]

Identifier: 187736428

GI number: 187736428

Start: 2364322

End: 2365239

Strand: Direct

Name: nanA1 [H]

Synonym: Amuc_1946

Alternate gene names: 187736428

Gene position: 2364322-2365239 (Clockwise)

Preceding gene: 187736427

Following gene: 187736429

Centisome position: 88.75

GC content: 58.93

Gene sequence:

>918_bases
ATGGACACGTCATTGAAACTCCACGGCCTGGTGGCCGCCGTACACACTCCGTTCAAGGCGGACGGTTCCCTGAACCCTTC
CAGCGTTGACGCCCAGGCCAAGTTGCTTGCCTCCCAGGGCATCAAGCTTGCTTTCATTACCGGCAGCACCGGGGAATCCT
CCTCCATGCAGCTTGAAGAACGCAAGGAAATCTATTCTGCCTGGAAGGAAGCCTCCGCCAAGCATGGCGTGGAAGTTATC
GCCCATACCGGTTCCAACAGCGTCTGGGACGCCCGGGAACTGGCCTCTTTTGCCCAGGAATGCGGATTCGTGGCCACCAG
TTCCCTGGCCCCGTCCTACTACAAGCCCGGCACTGTTCAGCGCCTGGTGGAATGCTGCGCCTTCGCCGCCTCCGGCGCTC
CCGACCTGCCCTATTATTACTACGATATCCCCGTGCTGACGGGCGTACGCTTCAATCCGGTGGATTTCATCAGGCTGGCC
AAGGAACAGATTCCAAATTTCGCAGGCATCAAATTCACCAATCCGGATCTGGCCCTGTACCAGACCACGCTGAATTACGA
CGAGACCGTGGATATTCCCTGGGGTGTGGACGAATGGTTTACGGGCGCCCTTTCCGTGGGGGCCAAGGGCGCTGTGGGGA
GCTCCTTCAACTTTGCTCCGGCCCTGTACCAGAAACTCATGAAAGCCTTTGCGGAAGGCGATGTGGAAACGGCGCGCGAC
TGCCAGTGGAAATCCGTTCAGATGATCAATATCCTGGCCTCCAAGGGCTATATGGGCTGCGCCAAGGCTCTGATGGGCTG
GCTGGGCGTCGATCTTGGCCCCGCCCGACTTCCGCAGGGCAACCCGACCGCAGATCAGCTGAAGGAACTCCGTTCCGAAC
TGGAAGGCATCGGCTTCTTCCAGTGGGCTTTAAACTGA

Upstream 100 bases:

>100_bases
TTTTTATGTGGTCTTTTTTTATTCAGAAAGATTCAGGGATTGTCTTTCCCAAGATGATTTGCAATCATCACCCCGAATTA
TTTTCATATCACTACATAAT

Downstream 100 bases:

>100_bases
ACCCCGGAACCTGACTGACATGGCTGCAACTCTTGATATGCCCTCTTTGGGCAAGTTCTACCGCCGCCAGCTTCTGGAAG
ACGTTCTGCCGTTCTGGTTC

Product: dihydrodipicolinate synthetase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase 1; N-acetylneuraminic acid aldolase 1; Sialate lyase 1; Sialic acid aldolase 1; Sialic acid lyase 1 [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MDTSLKLHGLVAAVHTPFKADGSLNPSSVDAQAKLLASQGIKLAFITGSTGESSSMQLEERKEIYSAWKEASAKHGVEVI
AHTGSNSVWDARELASFAQECGFVATSSLAPSYYKPGTVQRLVECCAFAASGAPDLPYYYYDIPVLTGVRFNPVDFIRLA
KEQIPNFAGIKFTNPDLALYQTTLNYDETVDIPWGVDEWFTGALSVGAKGAVGSSFNFAPALYQKLMKAFAEGDVETARD
CQWKSVQMINILASKGYMGCAKALMGWLGVDLGPARLPQGNPTADQLKELRSELEGIGFFQWALN

Sequences:

>Translated_305_residues
MDTSLKLHGLVAAVHTPFKADGSLNPSSVDAQAKLLASQGIKLAFITGSTGESSSMQLEERKEIYSAWKEASAKHGVEVI
AHTGSNSVWDARELASFAQECGFVATSSLAPSYYKPGTVQRLVECCAFAASGAPDLPYYYYDIPVLTGVRFNPVDFIRLA
KEQIPNFAGIKFTNPDLALYQTTLNYDETVDIPWGVDEWFTGALSVGAKGAVGSSFNFAPALYQKLMKAFAEGDVETARD
CQWKSVQMINILASKGYMGCAKALMGWLGVDLGPARLPQGNPTADQLKELRSELEGIGFFQWALN
>Mature_305_residues
MDTSLKLHGLVAAVHTPFKADGSLNPSSVDAQAKLLASQGIKLAFITGSTGESSSMQLEERKEIYSAWKEASAKHGVEVI
AHTGSNSVWDARELASFAQECGFVATSSLAPSYYKPGTVQRLVECCAFAASGAPDLPYYYYDIPVLTGVRFNPVDFIRLA
KEQIPNFAGIKFTNPDLALYQTTLNYDETVDIPWGVDEWFTGALSVGAKGAVGSSFNFAPALYQKLMKAFAEGDVETARD
CQWKSVQMINILASKGYMGCAKALMGWLGVDLGPARLPQGNPTADQLKELRSELEGIGFFQWALN

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=302, Percent_Identity=32.4503311258278, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1789620, Length=289, Percent_Identity=31.4878892733564, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082415, Length=255, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786463, Length=244, Percent_Identity=29.5081967213115, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788823, Length=249, Percent_Identity=25.7028112449799, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33032; Mature: 33032

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSLKLHGLVAAVHTPFKADGSLNPSSVDAQAKLLASQGIKLAFITGSTGESSSMQLEE
CCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
RKEIYSAWKEASAKHGVEVIAHTGSNSVWDARELASFAQECGFVATSSLAPSYYKPGTVQ
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
RLVECCAFAASGAPDLPYYYYDIPVLTGVRFNPVDFIRLAKEQIPNFAGIKFTNPDLALY
HHHHHHHHHCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCEEEE
QTTLNYDETVDIPWGVDEWFTGALSVGAKGAVGSSFNFAPALYQKLMKAFAEGDVETARD
EEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
CQWKSVQMINILASKGYMGCAKALMGWLGVDLGPARLPQGNPTADQLKELRSELEGIGFF
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEE
QWALN
EEECC
>Mature Secondary Structure
MDTSLKLHGLVAAVHTPFKADGSLNPSSVDAQAKLLASQGIKLAFITGSTGESSSMQLEE
CCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
RKEIYSAWKEASAKHGVEVIAHTGSNSVWDARELASFAQECGFVATSSLAPSYYKPGTVQ
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
RLVECCAFAASGAPDLPYYYYDIPVLTGVRFNPVDFIRLAKEQIPNFAGIKFTNPDLALY
HHHHHHHHHCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCEEEE
QTTLNYDETVDIPWGVDEWFTGALSVGAKGAVGSSFNFAPALYQKLMKAFAEGDVETARD
EEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHC
CQWKSVQMINILASKGYMGCAKALMGWLGVDLGPARLPQGNPTADQLKELRSELEGIGFF
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEE
QWALN
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]