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The map label for this gene is def [H]

Identifier: 187736350

GI number: 187736350

Start: 2269682

End: 2270230

Strand: Direct

Name: def [H]

Synonym: Amuc_1867

Alternate gene names: 187736350

Gene position: 2269682-2270230 (Clockwise)

Preceding gene: 187736349

Following gene: 187736353

Centisome position: 85.2

GC content: 49.73

Gene sequence:

>549_bases
ATGTTATTGGAAATAGCACAGTATGGAAATCCGGTATTGAAGGAAAAATGCCGCCCTGTAGAACATTTTGACGACAGCCT
GAAAACCCTGGCGGAAAATATGCTGGAAACCATGTACGCAGCAGAGGGCATTGGCCTGGCGGCTCCCCAGGTGAGCATTC
CCATTCAACTGGTAGTGATTGATATTCCGAAGGAGGAAGAGTCCGTCACCTGGCTTAAAGTGAACGGAGAGGACAAAGAG
CTTTCCGACATCATGCCCTTGATGTTTGCCAATCCCGTTCTGGAACCTTACGGCCCCATGCATCCTTTTCATGAAGGCTG
CCTGAGCGTGATGAAAATACGTGCTTCCGTCGTGAGGCCGGATTTTGTCAAGGCTACGGTGCTTCTGATTGACGGAAGAG
AAATAACGATTGACTGCAACGGCCTTCTGGCCAGATGCCTGCAGCATGAATGCGACCATTTGAACGGCATCCTTTTTGTG
GAGCGCGTTTCTTCCGCACAGAAGATTACCCTGCGCAACAAATTGAAGCGTCTGGCATTGGGATATTAG

Upstream 100 bases:

>100_bases
ACGTCTGTGGGTATTGACGCAGGTGAGGTTCCTGCCAGAATAGAAATCAACAAGCCCGGCGCACGGTAAGCGCTTTAACA
TATATATTATCCCCCTCCCG

Downstream 100 bases:

>100_bases
CTTTCCGTGACATCCCTCAGGCCCTTTATGGCAGCCAAAGGAGGGGGGGCAAAGAAGACCGCATTCCGGAACCGCAAAGT
TTTTACAGCCGGAAACCGTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLLEIAQYGNPVLKEKCRPVEHFDDSLKTLAENMLETMYAAEGIGLAAPQVSIPIQLVVIDIPKEEESVTWLKVNGEDKE
LSDIMPLMFANPVLEPYGPMHPFHEGCLSVMKIRASVVRPDFVKATVLLIDGREITIDCNGLLARCLQHECDHLNGILFV
ERVSSAQKITLRNKLKRLALGY

Sequences:

>Translated_182_residues
MLLEIAQYGNPVLKEKCRPVEHFDDSLKTLAENMLETMYAAEGIGLAAPQVSIPIQLVVIDIPKEEESVTWLKVNGEDKE
LSDIMPLMFANPVLEPYGPMHPFHEGCLSVMKIRASVVRPDFVKATVLLIDGREITIDCNGLLARCLQHECDHLNGILFV
ERVSSAQKITLRNKLKRLALGY
>Mature_182_residues
MLLEIAQYGNPVLKEKCRPVEHFDDSLKTLAENMLETMYAAEGIGLAAPQVSIPIQLVVIDIPKEEESVTWLKVNGEDKE
LSDIMPLMFANPVLEPYGPMHPFHEGCLSVMKIRASVVRPDFVKATVLLIDGREITIDCNGLLARCLQHECDHLNGILFV
ERVSSAQKITLRNKLKRLALGY

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=168, Percent_Identity=30.3571428571429, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1789682, Length=177, Percent_Identity=34.4632768361582, Blast_Score=100, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=32.5301204819277, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645726, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20382; Mature: 20382

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEIAQYGNPVLKEKCRPVEHFDDSLKTLAENMLETMYAAEGIGLAAPQVSIPIQLVVI
CEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
DIPKEEESVTWLKVNGEDKELSDIMPLMFANPVLEPYGPMHPFHEGCLSVMKIRASVVRP
ECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
DFVKATVLLIDGREITIDCNGLLARCLQHECDHLNGILFVERVSSAQKITLRNKLKRLAL
CHHEEEEEEEECCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHC
GY
CC
>Mature Secondary Structure
MLLEIAQYGNPVLKEKCRPVEHFDDSLKTLAENMLETMYAAEGIGLAAPQVSIPIQLVVI
CEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
DIPKEEESVTWLKVNGEDKELSDIMPLMFANPVLEPYGPMHPFHEGCLSVMKIRASVVRP
ECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
DFVKATVLLIDGREITIDCNGLLARCLQHECDHLNGILFVERVSSAQKITLRNKLKRLAL
CHHEEEEEEEECCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHC
GY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA