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The map label for this gene is ptsI [H]

Identifier: 187736243

GI number: 187736243

Start: 2138550

End: 2140328

Strand: Reverse

Name: ptsI [H]

Synonym: Amuc_1756

Alternate gene names: 187736243

Gene position: 2140328-2138550 (Counterclockwise)

Preceding gene: 187736244

Following gene: 187736242

Centisome position: 80.34

GC content: 59.25

Gene sequence:

>1779_bases
ATGATTCAGGAACCCATTTCAACGGAAGAAACCTGGCTTCAGGGCATTCCCGTTTCCCCCGGCATTGCTCTGGGCAAGAT
TAAAATTCAGATCAGCGGTTCCAAGGAACCCGTAGCGTACGATATTTCCCCGGAAGAAGTGGATGCCGAGCTGGTGCGCT
TCCACCGGGCGCTGCAAACTACTGCGGACCAGATCAGGACGCTGCGGGAACGCATGATCCAGATATCCGGAGAGAAAGAT
GCCTCCATCTTCGACGCCCACATCCTTCTCCTTCAGGATAAAATTATCCTCCAGCAGGTTCAGACGGAACTGCTCAAGCG
CCTCCAGAACGTGGAGCACATCTTTTATGTGGTCATGCAGAACTACATGGAAGTTCTGCGCCGTGTGGACGACCCCTACC
TGCGCGCCAAAACGGCGGACATGGAGGATGTCATGCAGCGGGTCATCAACAACCTCCGCAGCACGGAACCTCCTGAAGAC
GAGGAAGAAACGGAAAAAGACCAGGTTCTGGTGGCCTACGACCTGACCCCTTCCGACACGGCCGCCATGGATGCCAGCCT
GATTCACGGCTTTGCCACGGAAATAGGTTCCTCCGTTTCCCACACAGCCATTCTCGCCCGCTCCATGGGCATCCCCGCCG
TCGTGGGGCTTGACCAGGCCCTTCTGAAAGTGGAAAGCCACTCCCCCGCCATCCTGGACGGCTACAAGGGCGTGCTCATC
CTGAAACCCACGAAAGAAACGGAGGAATACTACCACCGCCTCCAGGTGGAAAAGGAAAAGGCCTACAAGGCGCTGGAAGC
GCTGCGCGGCCTGCCCACCATCACCAGGGACGGACGCAACATCCGCCTGTCCGTCAACGTGGAGTTCCCCCATGAATATT
CCGGCATCAAGGAGGTGGGAGCGGAAGGGGTAGGCCTGTTCCGCACGGAATTCTTTCTGCTGGGCAACCCATCCGGCATG
CCGGATGAAGAAGAACAGACGCGCTACTACAGAAAACTGGCGGAAGGCTGTTCCCCCCACGGCGTTATCTTCCGCACCCT
GGACTCAGGCGGTGACAAGCTGCCGTGCGAACAGCTCCGCACTCCGGAACCGAACCCCTTCCTTGGCTGGCGGGGCATCC
GCGTCTCCCTCAGCCGCCCGGAGCTGTTCAAACAGCAGCTCCGGGCCATTCTCCGTGCCTGCGCGGACACGCCCGGCTGC
GGCATCATGTTCCCCATGGTCTCCGGCTATACGGAAGTGGTCACAGCCAAGCACATCCTCCAGGAATGCCGGGAAGAACT
GGAAAGGAAGAACATCCCCTATGCGCGGGACTTGAAAGTGGGCATCATGATTGAAGTGCCCAGCGCCGCCATCATGACGG
ACGTGCTGGCGCGCGAGGTGGATTTCTTCTCCATAGGCACGAATGACCTCACCCAGTACACTATTGCCGTGGACCGGGTC
AACAACCGCGTCGCCAATATGTTCCGCCCCACGCATCCTGCCGTGATACGCATCATGGACATGACGATCACCGCCGGAGA
ACGGGAAAACATTCCCACGGCCATCTGTGGGGAAATGGCGGGGGACATCACCCTGCTTCCCCTGCTGATCGGCCTGGGGG
CCACCTCCATGTCCGTGGGGGTCCACCTTGTCCCCATCATCCGGTATGCCATCCGCAATCTGGACTACGGCCAGTGCCGG
GACATGGCGCAAAAGGCGCTCCAGGCCCCCAACAGCAGGTCTATCGTGGACTTGAGCACGGCCCTGGCCCGAAAATCCTA
TCCGGCCCTGTTTGAATAA

Upstream 100 bases:

>100_bases
GAAGCGCTGAACGCGCTGGAAGACCTGATTTACCGCAAGTTTGAAGAAGATTGAACACTCCGGCTCACGGCCATATGAGA
CCGGGCCATTAAGCCCGCAG

Downstream 100 bases:

>100_bases
GGCTCTTTCATCCCGGTACACGCCGCACCGGAAAAACGGGGCGTTCCCGAACAAACTGCCGCGGGAATACGAATCCTTGA
CAGAAACGTCCGTCTCATGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MIQEPISTEETWLQGIPVSPGIALGKIKIQISGSKEPVAYDISPEEVDAELVRFHRALQTTADQIRTLRERMIQISGEKD
ASIFDAHILLLQDKIILQQVQTELLKRLQNVEHIFYVVMQNYMEVLRRVDDPYLRAKTADMEDVMQRVINNLRSTEPPED
EEETEKDQVLVAYDLTPSDTAAMDASLIHGFATEIGSSVSHTAILARSMGIPAVVGLDQALLKVESHSPAILDGYKGVLI
LKPTKETEEYYHRLQVEKEKAYKALEALRGLPTITRDGRNIRLSVNVEFPHEYSGIKEVGAEGVGLFRTEFFLLGNPSGM
PDEEEQTRYYRKLAEGCSPHGVIFRTLDSGGDKLPCEQLRTPEPNPFLGWRGIRVSLSRPELFKQQLRAILRACADTPGC
GIMFPMVSGYTEVVTAKHILQECREELERKNIPYARDLKVGIMIEVPSAAIMTDVLAREVDFFSIGTNDLTQYTIAVDRV
NNRVANMFRPTHPAVIRIMDMTITAGERENIPTAICGEMAGDITLLPLLIGLGATSMSVGVHLVPIIRYAIRNLDYGQCR
DMAQKALQAPNSRSIVDLSTALARKSYPALFE

Sequences:

>Translated_592_residues
MIQEPISTEETWLQGIPVSPGIALGKIKIQISGSKEPVAYDISPEEVDAELVRFHRALQTTADQIRTLRERMIQISGEKD
ASIFDAHILLLQDKIILQQVQTELLKRLQNVEHIFYVVMQNYMEVLRRVDDPYLRAKTADMEDVMQRVINNLRSTEPPED
EEETEKDQVLVAYDLTPSDTAAMDASLIHGFATEIGSSVSHTAILARSMGIPAVVGLDQALLKVESHSPAILDGYKGVLI
LKPTKETEEYYHRLQVEKEKAYKALEALRGLPTITRDGRNIRLSVNVEFPHEYSGIKEVGAEGVGLFRTEFFLLGNPSGM
PDEEEQTRYYRKLAEGCSPHGVIFRTLDSGGDKLPCEQLRTPEPNPFLGWRGIRVSLSRPELFKQQLRAILRACADTPGC
GIMFPMVSGYTEVVTAKHILQECREELERKNIPYARDLKVGIMIEVPSAAIMTDVLAREVDFFSIGTNDLTQYTIAVDRV
NNRVANMFRPTHPAVIRIMDMTITAGERENIPTAICGEMAGDITLLPLLIGLGATSMSVGVHLVPIIRYAIRNLDYGQCR
DMAQKALQAPNSRSIVDLSTALARKSYPALFE
>Mature_592_residues
MIQEPISTEETWLQGIPVSPGIALGKIKIQISGSKEPVAYDISPEEVDAELVRFHRALQTTADQIRTLRERMIQISGEKD
ASIFDAHILLLQDKIILQQVQTELLKRLQNVEHIFYVVMQNYMEVLRRVDDPYLRAKTADMEDVMQRVINNLRSTEPPED
EEETEKDQVLVAYDLTPSDTAAMDASLIHGFATEIGSSVSHTAILARSMGIPAVVGLDQALLKVESHSPAILDGYKGVLI
LKPTKETEEYYHRLQVEKEKAYKALEALRGLPTITRDGRNIRLSVNVEFPHEYSGIKEVGAEGVGLFRTEFFLLGNPSGM
PDEEEQTRYYRKLAEGCSPHGVIFRTLDSGGDKLPCEQLRTPEPNPFLGWRGIRVSLSRPELFKQQLRAILRACADTPGC
GIMFPMVSGYTEVVTAKHILQECREELERKNIPYARDLKVGIMIEVPSAAIMTDVLAREVDFFSIGTNDLTQYTIAVDRV
NNRVANMFRPTHPAVIRIMDMTITAGERENIPTAICGEMAGDITLLPLLIGLGATSMSVGVHLVPIIRYAIRNLDYGQCR
DMAQKALQAPNSRSIVDLSTALARKSYPALFE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=35.4385964912281, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1789193, Length=566, Percent_Identity=33.9222614840989, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1788726, Length=574, Percent_Identity=34.1463414634146, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI48994992, Length=533, Percent_Identity=31.7073170731707, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1787994, Length=369, Percent_Identity=28.4552845528455, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI226510935, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 66161; Mature: 66161

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQEPISTEETWLQGIPVSPGIALGKIKIQISGSKEPVAYDISPEEVDAELVRFHRALQT
CCCCCCCCHHHHHCCCCCCCCEEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHH
TADQIRTLRERMIQISGEKDASIFDAHILLLQDKIILQQVQTELLKRLQNVEHIFYVVMQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYMEVLRRVDDPYLRAKTADMEDVMQRVINNLRSTEPPEDEEETEKDQVLVAYDLTPSDT
HHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCCCH
AAMDASLIHGFATEIGSSVSHTAILARSMGIPAVVGLDQALLKVESHSPAILDGYKGVLI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCEEECCCCEEE
LKPTKETEEYYHRLQVEKEKAYKALEALRGLPTITRDGRNIRLSVNVEFPHEYSGIKEVG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEECCHHHCCHHHHC
AEGVGLFRTEFFLLGNPSGMPDEEEQTRYYRKLAEGCSPHGVIFRTLDSGGDKLPCEQLR
CCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHCC
TPEPNPFLGWRGIRVSLSRPELFKQQLRAILRACADTPGCGIMFPMVSGYTEVVTAKHIL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCHHHHHHHHHHH
QECREELERKNIPYARDLKVGIMIEVPSAAIMTDVLAREVDFFSIGTNDLTQYTIAVDRV
HHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCCHHHHEEEHHHH
NNRVANMFRPTHPAVIRIMDMTITAGERENIPTAICGEMAGDITLLPLLIGLGATSMSVG
HHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCHHHCC
VHLVPIIRYAIRNLDYGQCRDMAQKALQAPNSRSIVDLSTALARKSYPALFE
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIQEPISTEETWLQGIPVSPGIALGKIKIQISGSKEPVAYDISPEEVDAELVRFHRALQT
CCCCCCCCHHHHHCCCCCCCCEEEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHH
TADQIRTLRERMIQISGEKDASIFDAHILLLQDKIILQQVQTELLKRLQNVEHIFYVVMQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYMEVLRRVDDPYLRAKTADMEDVMQRVINNLRSTEPPEDEEETEKDQVLVAYDLTPSDT
HHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCCCH
AAMDASLIHGFATEIGSSVSHTAILARSMGIPAVVGLDQALLKVESHSPAILDGYKGVLI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCEEECCCCEEE
LKPTKETEEYYHRLQVEKEKAYKALEALRGLPTITRDGRNIRLSVNVEFPHEYSGIKEVG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEECCHHHCCHHHHC
AEGVGLFRTEFFLLGNPSGMPDEEEQTRYYRKLAEGCSPHGVIFRTLDSGGDKLPCEQLR
CCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHCC
TPEPNPFLGWRGIRVSLSRPELFKQQLRAILRACADTPGCGIMFPMVSGYTEVVTAKHIL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCHHHHHHHHHHH
QECREELERKNIPYARDLKVGIMIEVPSAAIMTDVLAREVDFFSIGTNDLTQYTIAVDRV
HHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCCHHHHEEEHHHH
NNRVANMFRPTHPAVIRIMDMTITAGERENIPTAICGEMAGDITLLPLLIGLGATSMSVG
HHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCHHHCC
VHLVPIIRYAIRNLDYGQCRDMAQKALQAPNSRSIVDLSTALARKSYPALFE
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1551842; 1901791 [H]