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The map label for this gene is pfp [H]

Identifier: 187736158

GI number: 187736158

Start: 2025088

End: 2026332

Strand: Reverse

Name: pfp [H]

Synonym: Amuc_1671

Alternate gene names: 187736158

Gene position: 2026332-2025088 (Counterclockwise)

Preceding gene: 187736159

Following gene: 187736157

Centisome position: 76.06

GC content: 57.19

Gene sequence:

>1245_bases
ATGAACCCATTGAAAGGAGCATGCATCATCGGCCAGTCCGGCGGCCCCACCGCCGTCATCAACGCCAGCGCACTGGGAGC
CATTCAGTCCGCCCTGCAAAGCGAACAGGTCACGCGCGTGCTGGGAGCCGCCAACGGGATTGAGGGAGTGCTCAAAGAAC
GCCTGTTCGATATGGCCCGGGAAGACCCCGGAGAATTGGAATTGCTCAAATATACCCCAGCATCTGCCCTGGGTTCCTGC
CGCTACAAGATGGCAGCCCCGTCCGTGGATGATACGGATTACCGCCGTCTGCTGGAAATTTTCAGAAAATACGACGTACG
CTACTTTTTCTACAACGGAGGCAACGATTCCATGGATACCTGTAACAAGATATCCAAATTCATGCAGCAGTCCGGTTACG
AATGCCGCGTCATCGGAATTCCCAAGACCATTGACAATGACCTTCACGGCACGGACCACTGCCCCGGCTTCGGCTCTGCC
GCCAAATTTATCGCTACCTCCTGCATGGAAGTTCACCAGGACCTCCGCGTGTACGACAAGGGACGCGTCACCATTGTGGA
AATCATGGGCCGCCACGCCGGCTGGCTGGCCGGATCCGCAGCCCTGGCCACGTATGCGGGAGCAGGTCCGGACCTGGTTT
ACCTGCCGGAAGTTCCCTTCCGCATGGAGGAATTCTGGCAGGATGTGGACCGGATTTACAGGCAGAACGGCAGCTGCATG
GTCGCCGTCTCGGAAGGCGTGCAGTATGCAGACGGCCGCTTTGTTGCGGAATCCGGAGACAGGGACGTCTTCGGCCATAC
GCAGCTGGGAGGCCTGGGAGCCATGCTTGCGGAATCCGTCAAGCGACAAACCGGGGCCAAAGTCAGAAGCATTGAACTCT
CACTCCTCCAGCGCTGCGCCTCCCATGTAGCCTCCAAGACGGATATCGATGAAGCGTATATGGCGGGAAAAGCCGCCGTG
GAAGCCGCCGTTTCCGGCCAGACGGATAAAATGGTAGCCTTTGAACGAATCACGGAGAACGGCATTTATTCCTGCAAGAC
TAAATTGATAGGTCTGGAAGAAGTGGCCAATGTGGAAAAACTTGTTCCCCGCGAATGGATCAACGCCCGCGGCAATGGCG
TGGAACAGCCGTTCATTGATTACGTTCTTCCCCTGATCCAGGGAGAAACGGCCATGCAAAAGGAATGTTCCCTGCCCCGC
TTCGCGAAACTCAGGAAAGTGCCGGCAAAGCCGGAACAGCGGTAG

Upstream 100 bases:

>100_bases
CATCCCTCCGGGCTGCGGATAGGTTTGACTATCATTTCATTCCCTGCCTCCAACGCCGTTGAAAGGGGGCGCAACACAAC
TCTCTCATCCATTATTGCCC

Downstream 100 bases:

>100_bases
CAGTTTCTTCAGGGAAGCGTTTTTCCTCATCAACCCGGCCGGATCTGTCTGCCCCACGCTCTTGCAGGTAAACATCCGGC
TGGGGCTCTTCTCCCCGCCC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNPLKGACIIGQSGGPTAVINASALGAIQSALQSEQVTRVLGAANGIEGVLKERLFDMAREDPGELELLKYTPASALGSC
RYKMAAPSVDDTDYRRLLEIFRKYDVRYFFYNGGNDSMDTCNKISKFMQQSGYECRVIGIPKTIDNDLHGTDHCPGFGSA
AKFIATSCMEVHQDLRVYDKGRVTIVEIMGRHAGWLAGSAALATYAGAGPDLVYLPEVPFRMEEFWQDVDRIYRQNGSCM
VAVSEGVQYADGRFVAESGDRDVFGHTQLGGLGAMLAESVKRQTGAKVRSIELSLLQRCASHVASKTDIDEAYMAGKAAV
EAAVSGQTDKMVAFERITENGIYSCKTKLIGLEEVANVEKLVPREWINARGNGVEQPFIDYVLPLIQGETAMQKECSLPR
FAKLRKVPAKPEQR

Sequences:

>Translated_414_residues
MNPLKGACIIGQSGGPTAVINASALGAIQSALQSEQVTRVLGAANGIEGVLKERLFDMAREDPGELELLKYTPASALGSC
RYKMAAPSVDDTDYRRLLEIFRKYDVRYFFYNGGNDSMDTCNKISKFMQQSGYECRVIGIPKTIDNDLHGTDHCPGFGSA
AKFIATSCMEVHQDLRVYDKGRVTIVEIMGRHAGWLAGSAALATYAGAGPDLVYLPEVPFRMEEFWQDVDRIYRQNGSCM
VAVSEGVQYADGRFVAESGDRDVFGHTQLGGLGAMLAESVKRQTGAKVRSIELSLLQRCASHVASKTDIDEAYMAGKAAV
EAAVSGQTDKMVAFERITENGIYSCKTKLIGLEEVANVEKLVPREWINARGNGVEQPFIDYVLPLIQGETAMQKECSLPR
FAKLRKVPAKPEQR
>Mature_414_residues
MNPLKGACIIGQSGGPTAVINASALGAIQSALQSEQVTRVLGAANGIEGVLKERLFDMAREDPGELELLKYTPASALGSC
RYKMAAPSVDDTDYRRLLEIFRKYDVRYFFYNGGNDSMDTCNKISKFMQQSGYECRVIGIPKTIDNDLHGTDHCPGFGSA
AKFIATSCMEVHQDLRVYDKGRVTIVEIMGRHAGWLAGSAALATYAGAGPDLVYLPEVPFRMEEFWQDVDRIYRQNGSCM
VAVSEGVQYADGRFVAESGDRDVFGHTQLGGLGAMLAESVKRQTGAKVRSIELSLLQRCASHVASKTDIDEAYMAGKAAV
EAAVSGQTDKMVAFERITENGIYSCKTKLIGLEEVANVEKLVPREWINARGNGVEQPFIDYVLPLIQGETAMQKECSLPR
FAKLRKVPAKPEQR

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=267, Percent_Identity=27.7153558052434, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 45307; Mature: 45307

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLKGACIIGQSGGPTAVINASALGAIQSALQSEQVTRVLGAANGIEGVLKERLFDMAR
CCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EDPGELELLKYTPASALGSCRYKMAAPSVDDTDYRRLLEIFRKYDVRYFFYNGGNDSMDT
CCCCCEEEEEECCHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHH
CNKISKFMQQSGYECRVIGIPKTIDNDLHGTDHCPGFGSAAKFIATSCMEVHQDLRVYDK
HHHHHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GRVTIVEIMGRHAGWLAGSAALATYAGAGPDLVYLPEVPFRMEEFWQDVDRIYRQNGSCM
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEE
VAVSEGVQYADGRFVAESGDRDVFGHTQLGGLGAMLAESVKRQTGAKVRSIELSLLQRCA
EEECCCCEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SHVASKTDIDEAYMAGKAAVEAAVSGQTDKMVAFERITENGIYSCKTKLIGLEEVANVEK
HHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
LVPREWINARGNGVEQPFIDYVLPLIQGETAMQKECSLPRFAKLRKVPAKPEQR
HCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNPLKGACIIGQSGGPTAVINASALGAIQSALQSEQVTRVLGAANGIEGVLKERLFDMAR
CCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EDPGELELLKYTPASALGSCRYKMAAPSVDDTDYRRLLEIFRKYDVRYFFYNGGNDSMDT
CCCCCEEEEEECCHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHH
CNKISKFMQQSGYECRVIGIPKTIDNDLHGTDHCPGFGSAAKFIATSCMEVHQDLRVYDK
HHHHHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GRVTIVEIMGRHAGWLAGSAALATYAGAGPDLVYLPEVPFRMEEFWQDVDRIYRQNGSCM
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEE
VAVSEGVQYADGRFVAESGDRDVFGHTQLGGLGAMLAESVKRQTGAKVRSIELSLLQRCA
EEECCCCEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SHVASKTDIDEAYMAGKAAVEAAVSGQTDKMVAFERITENGIYSCKTKLIGLEEVANVEK
HHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
LVPREWINARGNGVEQPFIDYVLPLIQGETAMQKECSLPRFAKLRKVPAKPEQR
HCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]