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The map label for this gene is rpoD [H]

Identifier: 187736115

GI number: 187736115

Start: 1959219

End: 1961285

Strand: Direct

Name: rpoD [H]

Synonym: Amuc_1626

Alternate gene names: 187736115

Gene position: 1959219-1961285 (Clockwise)

Preceding gene: 187736114

Following gene: 187736116

Centisome position: 73.54

GC content: 55.25

Gene sequence:

>2067_bases
ATGTCCAAATCCGGTGACCCATCCCCCTCTTCTCCCAAGAAGACTTCCGCCAACAAAAAAGCTTCTGAATCCAAGGAAAA
AATAGCGGCCCGGCCCGCCGCAAAAAATTCCAAAACCGCTCCGAAGGCGGCTCCTGTCAAAAAAAACTCTGCCAAGGCTG
CCGCTCCGGCTGCGGAAAAGGCCGCCGCCAAGCCCGTTGCGGAAAAAAAGGCCCCGGCAAAAAAAGCGTCTCCGGCTTCT
GCCTCCAAGAAGGCGGCGCCGTCCGCCAAAAAGACTCCGGCAAAGGCGCCTGAAGCTGTCCCTGCCAAGAAAGCTCCAGC
CAAGGCTGCGGAGAAGAAGCCGGCTGAGAAGAAGGCCGCCAAACCTGCCGCGAAGGAGGAAACGCCCAAAAAATTAAGCG
AAATACTGGAAGAGGAACGCAAGAGGGCTGCCAGCCGCAAGATCACGAATCCGATTGACGCTCCGGAAATTCAGGAAAAA
ATCCGTGAACTTATCAAGCTCGCCAAGGAACAGGGGTATCTGACTTTTGACGATATTAATGACTCCCTTCCCAACGACAT
TGTCGATCAGCAGGATTACGAGGCCATCATGGACCGCCTGCGCAGCATGGCGTTTGACATCATTGACGCGTCTGATGTGG
ACAGCTACACGGACCGCACCCGCATCAGCACGGAAGAAGAGGACGAAGAGGAAAAGCTCGAAGCCAAGATGGACATTCTG
GATGATCCCGTCCGCATGTACCTGAAACAAATGGGCCAGGTTTCCCTGCTTACCCGTGAAGAGGAAGTGGCCATTTCCAA
GAGAATTGAGGACGCGGAACAGAACGTCCAGCGCTGTGTGCACCGCTTCGGTTTCATCGCGAATGCCTATCTGGACGTAG
CCTATCGCCTGCTCGACAATGAGGAACGCTTTGACCGCGTTATCCTTGACAAAAAGATAGACTCCCGTGAGCGCTATATG
AAAGGGCTGGCCCAGCTCTGCGCCCAGATACAGCAGACCCATCAGGACGCATCCGGTTCCTTCCGCAAGCTGTACCGCAG
CAAGGAGGCGGCCAAGTCCGTCAAGGCCCGCCAGGCCGAATTCGACAAAGTTGCCGGCGCTTTGGTGAAGTTCTTCGGTC
GCCTGTATTTCAAGCACAAGGTGATTGAGGATTTCTGTTCCATGATTGACGAGGCCCGCGACCGCGTGCTCCGCATGCAG
AAGAAGGTTGCCCTGGAACCGGACAACAAGGAACTCAAGGAACATCTGGCAGAGCTGGAGCTTCGCATGTGGATGACTGC
GGATGAGATGGGAGATGCCTACCAGGAACTCCGCAAATGGCTTCGCGAAGCCCGCAGGGCCAAGGATGAAATGGTGGAGG
CCAATCTGCGCCTGGTCATTTCCATTGCCAAAAAATATACCAACCGCGGCCTCTCCTTCCTGGATCTGATTCAGGAAGGC
AACATGGGCCTGATGAAAGCGGTGGAAAAATTTGAATACCGCCGCGGCTACAAATTCTCCACCTATGCCACCTGGTGGAT
TCGCCAGGCCATCACCCGCTCCATTGCAGACCAGGCCCGCACCATCCGCATTCCCGTGCACATGATTGAAACCATCAATA
AATTGATGCGCGTGCAAAAGCAGTTGGTGCAGGAGTACGGTAGGGAACCCACTCCGGAAGAAATCGCGGAAGAAATCCAT
CTGCCTGTGGAACGCGTGCGTTCCGTTTTGAAAATGGCCCAGCAGCCCATTTCCCTGCAAGCCCCCGTGGGGGATTCCGA
CGATACCTCCTTTGGTGACTTTATTGAAGACAAGGCGGCGGAAAATCCCATGGAGGAAGCCTCCTTCTCTTTCCTGAAAG
AAAAAATCAAGGATGTTCTGGACACCCTCACGGATCGTGAACGCGAAGTTATTGAACAGCGCTTCGGCCTCCGGGACGGC
AGTCCCCGCACTCTGGAGGAAGTGGGCCGCCAGTTCAGCGTCACCCGCGAACGCATTCGCCAGATTGAAGCCAAAGCCCT
CCGCAAGCTGCGCCATCCCACGCGCATCAGCAAGATCAAGGGATTCCTGGAAATGACGGAATCCTAA

Upstream 100 bases:

>100_bases
AACTTAGTGTTGACTTGAAGCGTTTGCTGGGTACAATGTAGTGCCTCGATACCTCCGGGTATTGATATAATGGTGAATTG
TGCCCTCTTCTTTTCAAGAT

Downstream 100 bases:

>100_bases
CCGGATTTGCGCCGCATGCCGTGCTACGCTTTGTGGTCATGAAGACTTGCGTAGCTGCGGTATGCAGCATGTTCATGTTA
CTGGTTTCCTGTTCCGGCAG

Product: RNA polymerase, sigma 70 subunit, RpoD family

Products: NA

Alternate protein names: Sigma-A; Sigma-66; Sigma-70 [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSKSGDPSPSSPKKTSANKKASESKEKIAARPAAKNSKTAPKAAPVKKNSAKAAAPAAEKAAAKPVAEKKAPAKKASPAS
ASKKAAPSAKKTPAKAPEAVPAKKAPAKAAEKKPAEKKAAKPAAKEETPKKLSEILEEERKRAASRKITNPIDAPEIQEK
IRELIKLAKEQGYLTFDDINDSLPNDIVDQQDYEAIMDRLRSMAFDIIDASDVDSYTDRTRISTEEEDEEEKLEAKMDIL
DDPVRMYLKQMGQVSLLTREEEVAISKRIEDAEQNVQRCVHRFGFIANAYLDVAYRLLDNEERFDRVILDKKIDSRERYM
KGLAQLCAQIQQTHQDASGSFRKLYRSKEAAKSVKARQAEFDKVAGALVKFFGRLYFKHKVIEDFCSMIDEARDRVLRMQ
KKVALEPDNKELKEHLAELELRMWMTADEMGDAYQELRKWLREARRAKDEMVEANLRLVISIAKKYTNRGLSFLDLIQEG
NMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQKQLVQEYGREPTPEEIAEEIH
LPVERVRSVLKMAQQPISLQAPVGDSDDTSFGDFIEDKAAENPMEEASFSFLKEKIKDVLDTLTDREREVIEQRFGLRDG
SPRTLEEVGRQFSVTRERIRQIEAKALRKLRHPTRISKIKGFLEMTES

Sequences:

>Translated_688_residues
MSKSGDPSPSSPKKTSANKKASESKEKIAARPAAKNSKTAPKAAPVKKNSAKAAAPAAEKAAAKPVAEKKAPAKKASPAS
ASKKAAPSAKKTPAKAPEAVPAKKAPAKAAEKKPAEKKAAKPAAKEETPKKLSEILEEERKRAASRKITNPIDAPEIQEK
IRELIKLAKEQGYLTFDDINDSLPNDIVDQQDYEAIMDRLRSMAFDIIDASDVDSYTDRTRISTEEEDEEEKLEAKMDIL
DDPVRMYLKQMGQVSLLTREEEVAISKRIEDAEQNVQRCVHRFGFIANAYLDVAYRLLDNEERFDRVILDKKIDSRERYM
KGLAQLCAQIQQTHQDASGSFRKLYRSKEAAKSVKARQAEFDKVAGALVKFFGRLYFKHKVIEDFCSMIDEARDRVLRMQ
KKVALEPDNKELKEHLAELELRMWMTADEMGDAYQELRKWLREARRAKDEMVEANLRLVISIAKKYTNRGLSFLDLIQEG
NMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQKQLVQEYGREPTPEEIAEEIH
LPVERVRSVLKMAQQPISLQAPVGDSDDTSFGDFIEDKAAENPMEEASFSFLKEKIKDVLDTLTDREREVIEQRFGLRDG
SPRTLEEVGRQFSVTRERIRQIEAKALRKLRHPTRISKIKGFLEMTES
>Mature_687_residues
SKSGDPSPSSPKKTSANKKASESKEKIAARPAAKNSKTAPKAAPVKKNSAKAAAPAAEKAAAKPVAEKKAPAKKASPASA
SKKAAPSAKKTPAKAPEAVPAKKAPAKAAEKKPAEKKAAKPAAKEETPKKLSEILEEERKRAASRKITNPIDAPEIQEKI
RELIKLAKEQGYLTFDDINDSLPNDIVDQQDYEAIMDRLRSMAFDIIDASDVDSYTDRTRISTEEEDEEEKLEAKMDILD
DPVRMYLKQMGQVSLLTREEEVAISKRIEDAEQNVQRCVHRFGFIANAYLDVAYRLLDNEERFDRVILDKKIDSRERYMK
GLAQLCAQIQQTHQDASGSFRKLYRSKEAAKSVKARQAEFDKVAGALVKFFGRLYFKHKVIEDFCSMIDEARDRVLRMQK
KVALEPDNKELKEHLAELELRMWMTADEMGDAYQELRKWLREARRAKDEMVEANLRLVISIAKKYTNRGLSFLDLIQEGN
MGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQKQLVQEYGREPTPEEIAEEIHL
PVERVRSVLKMAQQPISLQAPVGDSDDTSFGDFIEDKAAENPMEEASFSFLKEKIKDVLDTLTDREREVIEQRFGLRDGS
PRTLEEVGRQFSVTRERIRQIEAKALRKLRHPTRISKIKGFLEMTES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=269, Percent_Identity=63.5687732342007, Blast_Score=354, Evalue=9e-99,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=43.5897435897436, Blast_Score=215, Evalue=8e-57,
Organism=Escherichia coli, GI1789871, Length=239, Percent_Identity=30.5439330543933, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788231, Length=210, Percent_Identity=28.0952380952381, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 78263; Mature: 78132

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSGDPSPSSPKKTSANKKASESKEKIAARPAAKNSKTAPKAAPVKKNSAKAAAPAAEK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AAAKPVAEKKAPAKKASPASASKKAAPSAKKTPAKAPEAVPAKKAPAKAAEKKPAEKKAA
HHHCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHC
KPAAKEETPKKLSEILEEERKRAASRKITNPIDAPEIQEKIRELIKLAKEQGYLTFDDIN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCC
DSLPNDIVDQQDYEAIMDRLRSMAFDIIDASDVDSYTDRTRISTEEEDEEEKLEAKMDIL
CCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
DDPVRMYLKQMGQVSLLTREEEVAISKRIEDAEQNVQRCVHRFGFIANAYLDVAYRLLDN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EERFDRVILDKKIDSRERYMKGLAQLCAQIQQTHQDASGSFRKLYRSKEAAKSVKARQAE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FDKVAGALVKFFGRLYFKHKVIEDFCSMIDEARDRVLRMQKKVALEPDNKELKEHLAELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LRMWMTADEMGDAYQELRKWLREARRAKDEMVEANLRLVISIAKKYTNRGLSFLDLIQEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
NMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHH
QLVQEYGREPTPEEIAEEIHLPVERVRSVLKMAQQPISLQAPVGDSDDTSFGDFIEDKAA
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHH
ENPMEEASFSFLKEKIKDVLDTLTDREREVIEQRFGLRDGSPRTLEEVGRQFSVTRERIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
QIEAKALRKLRHPTRISKIKGFLEMTES
HHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSGDPSPSSPKKTSANKKASESKEKIAARPAAKNSKTAPKAAPVKKNSAKAAAPAAEK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AAAKPVAEKKAPAKKASPASASKKAAPSAKKTPAKAPEAVPAKKAPAKAAEKKPAEKKAA
HHHCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHC
KPAAKEETPKKLSEILEEERKRAASRKITNPIDAPEIQEKIRELIKLAKEQGYLTFDDIN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCC
DSLPNDIVDQQDYEAIMDRLRSMAFDIIDASDVDSYTDRTRISTEEEDEEEKLEAKMDIL
CCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
DDPVRMYLKQMGQVSLLTREEEVAISKRIEDAEQNVQRCVHRFGFIANAYLDVAYRLLDN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EERFDRVILDKKIDSRERYMKGLAQLCAQIQQTHQDASGSFRKLYRSKEAAKSVKARQAE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FDKVAGALVKFFGRLYFKHKVIEDFCSMIDEARDRVLRMQKKVALEPDNKELKEHLAELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LRMWMTADEMGDAYQELRKWLREARRAKDEMVEANLRLVISIAKKYTNRGLSFLDLIQEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
NMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHH
QLVQEYGREPTPEEIAEEIHLPVERVRSVLKMAQQPISLQAPVGDSDDTSFGDFIEDKAA
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHH
ENPMEEASFSFLKEKIKDVLDTLTDREREVIEQRFGLRDGSPRTLEEVGRQFSVTRERIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
QIEAKALRKLRHPTRISKIKGFLEMTES
HHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]