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The map label for this gene is vanX [H]

Identifier: 187736116

GI number: 187736116

Start: 1961324

End: 1962064

Strand: Direct

Name: vanX [H]

Synonym: Amuc_1627

Alternate gene names: 187736116

Gene position: 1961324-1962064 (Clockwise)

Preceding gene: 187736115

Following gene: 187736117

Centisome position: 73.62

GC content: 53.71

Gene sequence:

>741_bases
ATGAAGACTTGCGTAGCTGCGGTATGCAGCATGTTCATGTTACTGGTTTCCTGTTCCGGCAGCAGGGAATTCAAACCGGC
GTTCAATCAGCCCCTTCAGCCCGTGATGCAGCCGGGCTTTGTGTATGTGGATCAGGTGGCTCCGCTGGTGAAGACTAATT
TGAAATATGCCGGGTATGACAATTTTGTAGGGCGTCCGCTGGACGGTTACCACGGTCGGCGCGCCATCCTGCGCACCCAG
GCGGCGCAGGCGTTGAAAAAAGTCTCGGAAGATTTGTATCGTCAGGGTTACCTGCTGGAAATTTATGACGCCTACCGTCC
CCATACCGCCGTGCTGGACATTTGCAAATGGGGAGCTGACGACGGGGACCAAAAGATGAAGTCCCGGTATTATCCCCATA
TTGACAAGGCCAAGGTGTTCGGAGACCACTATGTGCGCGATTTTTCGGAACATTCCCGGGGTGTGGCTGTGGATGTTTCC
CTGCTGTACGCCAAGACCGGAAAGCCGGTGGATATGGGCGGCCATCATGACCTGCTGGATCCCAGTTCCACCACAGACTC
CACATTAGTGACTCCGTCCCAACGCAGAAACAGGATGATTCTGAAAACGGCTTTTGAAAAACAGGGTTTTGCCAATTATG
CTCCGGAATGGTGGCACTATCGCCTGTTGAATGAGCCTGAACCTACCCTGCATTATTACTTTCCCGTGTGGGACGGCATG
ACTTCCGCGGGCAGGCCATAA

Upstream 100 bases:

>100_bases
AGCTGCGCCATCCCACGCGCATCAGCAAGATCAAGGGATTCCTGGAAATGACGGAATCCTAACCGGATTTGCGCCGCATG
CCGTGCTACGCTTTGTGGTC

Downstream 100 bases:

>100_bases
AAAAATTTTACATATCAAATTTTGTAAAAATTTGTTTGTTAGCTGAGTGGTCGGCTCCGTGCTGATTCCTATCAAGCAGA
ATGTAGAAGATCGTACTGAC

Product: peptidase M15D vanX D-ala-D-ala dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKTCVAAVCSMFMLLVSCSGSREFKPAFNQPLQPVMQPGFVYVDQVAPLVKTNLKYAGYDNFVGRPLDGYHGRRAILRTQ
AAQALKKVSEDLYRQGYLLEIYDAYRPHTAVLDICKWGADDGDQKMKSRYYPHIDKAKVFGDHYVRDFSEHSRGVAVDVS
LLYAKTGKPVDMGGHHDLLDPSSTTDSTLVTPSQRRNRMILKTAFEKQGFANYAPEWWHYRLLNEPEPTLHYYFPVWDGM
TSAGRP

Sequences:

>Translated_246_residues
MKTCVAAVCSMFMLLVSCSGSREFKPAFNQPLQPVMQPGFVYVDQVAPLVKTNLKYAGYDNFVGRPLDGYHGRRAILRTQ
AAQALKKVSEDLYRQGYLLEIYDAYRPHTAVLDICKWGADDGDQKMKSRYYPHIDKAKVFGDHYVRDFSEHSRGVAVDVS
LLYAKTGKPVDMGGHHDLLDPSSTTDSTLVTPSQRRNRMILKTAFEKQGFANYAPEWWHYRLLNEPEPTLHYYFPVWDGM
TSAGRP
>Mature_246_residues
MKTCVAAVCSMFMLLVSCSGSREFKPAFNQPLQPVMQPGFVYVDQVAPLVKTNLKYAGYDNFVGRPLDGYHGRRAILRTQ
AAQALKKVSEDLYRQGYLLEIYDAYRPHTAVLDICKWGADDGDQKMKSRYYPHIDKAKVFGDHYVRDFSEHSRGVAVDVS
LLYAKTGKPVDMGGHHDLLDPSSTTDSTLVTPSQRRNRMILKTAFEKQGFANYAPEWWHYRLLNEPEPTLHYYFPVWDGM
TSAGRP

Specific function: Hydrolyzes the cellular pool of D-Ala-D-Ala dipeptides. May play a role in immunity or defense against glycopeptide antibiotics (perhaps at a moderate level) in the soil environment. Might confer vancomycin resistance to S.coelicolor [H]

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=180, Percent_Identity=32.2222222222222, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 28010; Mature: 28010

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTCVAAVCSMFMLLVSCSGSREFKPAFNQPLQPVMQPGFVYVDQVAPLVKTNLKYAGYD
CCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHCCCCEEHHHHHHHHHHCCCCCCCC
NFVGRPLDGYHGRRAILRTQAAQALKKVSEDLYRQGYLLEIYDAYRPHTAVLDICKWGAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHCCCC
DGDQKMKSRYYPHIDKAKVFGDHYVRDFSEHSRGVAVDVSLLYAKTGKPVDMGGHHDLLD
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCC
PSSTTDSTLVTPSQRRNRMILKTAFEKQGFANYAPEWWHYRLLNEPEPTLHYYFPVWDGM
CCCCCCCEEECCHHHHCEEHHHHHHHHCCCCCCCCCHHEEEECCCCCCCEEEEEECCCCC
TSAGRP
CCCCCC
>Mature Secondary Structure
MKTCVAAVCSMFMLLVSCSGSREFKPAFNQPLQPVMQPGFVYVDQVAPLVKTNLKYAGYD
CCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHCCCCEEHHHHHHHHHHCCCCCCCC
NFVGRPLDGYHGRRAILRTQAAQALKKVSEDLYRQGYLLEIYDAYRPHTAVLDICKWGAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHCCCC
DGDQKMKSRYYPHIDKAKVFGDHYVRDFSEHSRGVAVDVSLLYAKTGKPVDMGGHHDLLD
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCC
PSSTTDSTLVTPSQRRNRMILKTAFEKQGFANYAPEWWHYRLLNEPEPTLHYYFPVWDGM
CCCCCCCEEECCHHHHCEEHHHHHHHHCCCCCCCCCHHEEEECCCCCCCEEEEEECCCCC
TSAGRP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11881895; 12000953 [H]