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The map label for this gene is nuoN [H]

Identifier: 187736093

GI number: 187736093

Start: 1928195

End: 1929577

Strand: Reverse

Name: nuoN [H]

Synonym: Amuc_1604

Alternate gene names: 187736093

Gene position: 1929577-1928195 (Counterclockwise)

Preceding gene: 187736094

Following gene: 187736092

Centisome position: 72.43

GC content: 57.85

Gene sequence:

>1383_bases
ATGCAAGCGTATATTCCGGAATTCATCCTGGCCGGCCTGGCAATGCTTTTGCTTCTGGCGGAGACCTTCTGCAAAAAAAC
GCCCAAATTTATTTTCGGGCTGATTGGCGCGGCGGGAACCCTGGCGATGCTTCCCTTCTACATGGAAGGGCTCTATGACA
ACATCTACATCATCCTGGCGCTCGTCGCCACAGCAGTCACCCTGTTGCTTTCCGTAGATTTCCGGGCCGTCATCAACCTT
TCCTCCAATGATTCCAAATCCCAGGACGGAACGGGGGAATTCTACATCCTGCCCCTGCTGGCCTGCGTAGGCATCACCTC
CCTGTGCAAGGCATCCAACCTAGTGGAACTGTTCGTCTCCCTGGAAGTGCTCACCCTGAGTTCCTTCATCATGGTAGGCT
ACTTCCGGCGGAACCTGGGTTCCACGGAAGCGGGCATCAAGTACCTGATTCTGGGCGCCGTCAGCACGGGGTTTCTCGTT
TTCGGCCTGGCCTGGTATTTCGGCATTACGGGAACATTCATTTATAATGAAACGATCGTCAGCCACGCCCTGGCCGGCCA
GACAGCCCCCGCCATGTACCTGGCCCTCGCCATGCTGCTGCTCGGTACAGCATTCAAAATCGGTGCAGTTCCCATGCAGC
TGTGGATTCCGGATGTGTACCAGGGCGCTCCCACTCCGGTGACAGCTTTCCTTTCCGTAGCCTCCAAGGTAGCCGGATTC
GCCCTGTTCGGCATCATCCTGGCCCCATTCGCCGTGCTGCCTCCCGTCGAATTCGTCGTAGCCCTGATGGCCGCCGCCAC
CCTGCTGGTGGGCAACCTGGGCGCCATTCCCCAGACGAACCTGAAACGCATGATGGGGTATTCTTCCATTGCACAGGCAG
GCTTCATTCTTCCCCTGTTTATCGGTACTGTGGACGGCAAGCTGGCTCCGAACGCCCCGTTCTACCTGGCTGTGTACCTG
GTCATGACCTTCGGCGCCTTTTTCGCCCTTGCCATGATCCGCATCCAGCGCGGGAGCGAGGAAATTTCCGCGTTCCGCGG
TCTGGGCAAAACCAATCCCCGGCTGGCGCTGGCGGTCACCATCATGTTCGCCTCCCTGGCGGGCGTGCCGCTGACGGCCG
GATTTTTCGCCAAAATGATTTCTTTCGTGCACGTCATCAATACGGGTCTGTATCTGGGTTGGATGCTCCCCGTCATGATC
GTCTGCGCTGCATCCGGGTTTTACTATTACTTCAAGGTCATCCGCTCCATGTACTGGGACAAGCCCGCAGAAAACGCAGA
ACCCGTGCAGGTACCGGCCATTTCCGGAGTCATGCTGGCCGCGTTTTCCATCTTCATCGTGCTGGGGGGCCTGATGCCCC
TGTTCCTGAACCCCATCCGGTAA

Upstream 100 bases:

>100_bases
TGGCGGTCTTCGGCTTCTTCCCCACGCTGGTGCTCCAGTTTTTCTCCTGATTTCCTCCCTTCCGAATACAATCCTAGTCT
AACCTGATTCTCTTTTACCC

Downstream 100 bases:

>100_bases
CAAACGCGTCCTTTTCCCCCATTCTTCCGAAGGCCGTTTCCCGAATTTTTCGCAGAACGGCCTTCCCCATTCAGGCAAAG
CTGTATGTTCCGCGTCGGCT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MQAYIPEFILAGLAMLLLLAETFCKKTPKFIFGLIGAAGTLAMLPFYMEGLYDNIYIILALVATAVTLLLSVDFRAVINL
SSNDSKSQDGTGEFYILPLLACVGITSLCKASNLVELFVSLEVLTLSSFIMVGYFRRNLGSTEAGIKYLILGAVSTGFLV
FGLAWYFGITGTFIYNETIVSHALAGQTAPAMYLALAMLLLGTAFKIGAVPMQLWIPDVYQGAPTPVTAFLSVASKVAGF
ALFGIILAPFAVLPPVEFVVALMAAATLLVGNLGAIPQTNLKRMMGYSSIAQAGFILPLFIGTVDGKLAPNAPFYLAVYL
VMTFGAFFALAMIRIQRGSEEISAFRGLGKTNPRLALAVTIMFASLAGVPLTAGFFAKMISFVHVINTGLYLGWMLPVMI
VCAASGFYYYFKVIRSMYWDKPAENAEPVQVPAISGVMLAAFSIFIVLGGLMPLFLNPIR

Sequences:

>Translated_460_residues
MQAYIPEFILAGLAMLLLLAETFCKKTPKFIFGLIGAAGTLAMLPFYMEGLYDNIYIILALVATAVTLLLSVDFRAVINL
SSNDSKSQDGTGEFYILPLLACVGITSLCKASNLVELFVSLEVLTLSSFIMVGYFRRNLGSTEAGIKYLILGAVSTGFLV
FGLAWYFGITGTFIYNETIVSHALAGQTAPAMYLALAMLLLGTAFKIGAVPMQLWIPDVYQGAPTPVTAFLSVASKVAGF
ALFGIILAPFAVLPPVEFVVALMAAATLLVGNLGAIPQTNLKRMMGYSSIAQAGFILPLFIGTVDGKLAPNAPFYLAVYL
VMTFGAFFALAMIRIQRGSEEISAFRGLGKTNPRLALAVTIMFASLAGVPLTAGFFAKMISFVHVINTGLYLGWMLPVMI
VCAASGFYYYFKVIRSMYWDKPAENAEPVQVPAISGVMLAAFSIFIVLGGLMPLFLNPIR
>Mature_460_residues
MQAYIPEFILAGLAMLLLLAETFCKKTPKFIFGLIGAAGTLAMLPFYMEGLYDNIYIILALVATAVTLLLSVDFRAVINL
SSNDSKSQDGTGEFYILPLLACVGITSLCKASNLVELFVSLEVLTLSSFIMVGYFRRNLGSTEAGIKYLILGAVSTGFLV
FGLAWYFGITGTFIYNETIVSHALAGQTAPAMYLALAMLLLGTAFKIGAVPMQLWIPDVYQGAPTPVTAFLSVASKVAGF
ALFGIILAPFAVLPPVEFVVALMAAATLLVGNLGAIPQTNLKRMMGYSSIAQAGFILPLFIGTVDGKLAPNAPFYLAVYL
VMTFGAFFALAMIRIQRGSEEISAFRGLGKTNPRLALAVTIMFASLAGVPLTAGFFAKMISFVHVINTGLYLGWMLPVMI
VCAASGFYYYFKVIRSMYWDKPAENAEPVQVPAISGVMLAAFSIFIVLGGLMPLFLNPIR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=311, Percent_Identity=24.4372990353698, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI145693160, Length=399, Percent_Identity=34.5864661654135, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788614, Length=310, Percent_Identity=28.3870967741935, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788829, Length=359, Percent_Identity=25.0696378830084, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788831, Length=209, Percent_Identity=26.7942583732057, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 49388; Mature: 49388

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAYIPEFILAGLAMLLLLAETFCKKTPKFIFGLIGAAGTLAMLPFYMEGLYDNIYIILA
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVATAVTLLLSVDFRAVINLSSNDSKSQDGTGEFYILPLLACVGITSLCKASNLVELFVS
HHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEVLTLSSFIMVGYFRRNLGSTEAGIKYLILGAVSTGFLVFGLAWYFGITGTFIYNETIV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHH
SHALAGQTAPAMYLALAMLLLGTAFKIGAVPMQLWIPDVYQGAPTPVTAFLSVASKVAGF
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHH
ALFGIILAPFAVLPPVEFVVALMAAATLLVGNLGAIPQTNLKRMMGYSSIAQAGFILPLF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHH
IGTVDGKLAPNAPFYLAVYLVMTFGAFFALAMIRIQRGSEEISAFRGLGKTNPRLALAVT
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHH
IMFASLAGVPLTAGFFAKMISFVHVINTGLYLGWMLPVMIVCAASGFYYYFKVIRSMYWD
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
KPAENAEPVQVPAISGVMLAAFSIFIVLGGLMPLFLNPIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQAYIPEFILAGLAMLLLLAETFCKKTPKFIFGLIGAAGTLAMLPFYMEGLYDNIYIILA
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVATAVTLLLSVDFRAVINLSSNDSKSQDGTGEFYILPLLACVGITSLCKASNLVELFVS
HHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEVLTLSSFIMVGYFRRNLGSTEAGIKYLILGAVSTGFLVFGLAWYFGITGTFIYNETIV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHH
SHALAGQTAPAMYLALAMLLLGTAFKIGAVPMQLWIPDVYQGAPTPVTAFLSVASKVAGF
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHH
ALFGIILAPFAVLPPVEFVVALMAAATLLVGNLGAIPQTNLKRMMGYSSIAQAGFILPLF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHH
IGTVDGKLAPNAPFYLAVYLVMTFGAFFALAMIRIQRGSEEISAFRGLGKTNPRLALAVT
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHH
IMFASLAGVPLTAGFFAKMISFVHVINTGLYLGWMLPVMIVCAASGFYYYFKVIRSMYWD
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
KPAENAEPVQVPAISGVMLAAFSIFIVLGGLMPLFLNPIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA