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The map label for this gene is yibK [C]

Identifier: 187736063

GI number: 187736063

Start: 1897485

End: 1897949

Strand: Reverse

Name: yibK [C]

Synonym: Amuc_1574

Alternate gene names: 187736063

Gene position: 1897949-1897485 (Counterclockwise)

Preceding gene: 187736065

Following gene: 187736060

Centisome position: 71.24

GC content: 57.63

Gene sequence:

>465_bases
ATGCAGCCCCGTTTCCACATCGTCATGTTCCATCCGGAAATTCCGCATAATACAGGAGCTGCCGGACGGTTGGCGCTGGC
AACGGGTTCCAGGCTCCATTTGATCAAACCTTTGGGGTTCAGTCTGGATGAAAAGCATGTGCGCCGTACGGGTCTGGACT
ATTGGGCCAAGGTGGACCTGCATGTCTGGGACAGCCTGGAGGAATTGAAGCAGGCGGCAGCCCCCGGCGCGCAGTTCTGG
TACCTGAGCACCAAGGCGCCACGTTCCCATTGGGATGCGGTTTTCCACGCAGGGGACTATCTGGTTTTTGGACCGGAATC
CCGCGGATTGCCGGAAAGCATGCTGAGAGAACACGCAGATACCGCCCTGACTATTCCGATGCCCGGAGAAGGAGCCCGCA
GCCTGAATTTATCCACAGCCGTAGCTGTCGTCCTTTATGAAGGACTGCGCCAGACCGGCATTTGA

Upstream 100 bases:

>100_bases
AAATATACGATGTCTCTCATCGGGTGAAAACGTACATTATTTGCCACTTTTGGACAAGTCCCACTTGAACGGGAGGCCTG
TTTGTGATGAGAATAAGCGC

Downstream 100 bases:

>100_bases
CGAGCCCCCCGCCTGCCTCTCCACATCGGCATGGATGTTCTGCGGGTTCCACCACCAAAGAACACAGGGCCATGAAGAGG
CGGGTTCCGCGTCTTTCCTC

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MQPRFHIVMFHPEIPHNTGAAGRLALATGSRLHLIKPLGFSLDEKHVRRTGLDYWAKVDLHVWDSLEELKQAAAPGAQFW
YLSTKAPRSHWDAVFHAGDYLVFGPESRGLPESMLREHADTALTIPMPGEGARSLNLSTAVAVVLYEGLRQTGI

Sequences:

>Translated_154_residues
MQPRFHIVMFHPEIPHNTGAAGRLALATGSRLHLIKPLGFSLDEKHVRRTGLDYWAKVDLHVWDSLEELKQAAAPGAQFW
YLSTKAPRSHWDAVFHAGDYLVFGPESRGLPESMLREHADTALTIPMPGEGARSLNLSTAVAVVLYEGLRQTGI
>Mature_154_residues
MQPRFHIVMFHPEIPHNTGAAGRLALATGSRLHLIKPLGFSLDEKHVRRTGLDYWAKVDLHVWDSLEELKQAAAPGAQFW
YLSTKAPRSHWDAVFHAGDYLVFGPESRGLPESMLREHADTALTIPMPGEGARSLNLSTAVAVVLYEGLRQTGI

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=44, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17091; Mature: 17091

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPRFHIVMFHPEIPHNTGAAGRLALATGSRLHLIKPLGFSLDEKHVRRTGLDYWAKVDL
CCCCEEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHCCCCEEEEEEH
HVWDSLEELKQAAAPGAQFWYLSTKAPRSHWDAVFHAGDYLVFGPESRGLPESMLREHAD
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHEEECCCEEEECCCCCCCCHHHHHHCCC
TALTIPMPGEGARSLNLSTAVAVVLYEGLRQTGI
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPRFHIVMFHPEIPHNTGAAGRLALATGSRLHLIKPLGFSLDEKHVRRTGLDYWAKVDL
CCCCEEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHCCCCEEEEEEH
HVWDSLEELKQAAAPGAQFWYLSTKAPRSHWDAVFHAGDYLVFGPESRGLPESMLREHAD
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHEEECCCEEEECCCCCCCCHHHHHHCCC
TALTIPMPGEGARSLNLSTAVAVVLYEGLRQTGI
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA