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The map label for this gene is smpB [H]

Identifier: 187736065

GI number: 187736065

Start: 1898780

End: 1899238

Strand: Reverse

Name: smpB [H]

Synonym: Amuc_1576

Alternate gene names: 187736065

Gene position: 1899238-1898780 (Counterclockwise)

Preceding gene: 187736066

Following gene: 187736063

Centisome position: 71.29

GC content: 56.86

Gene sequence:

>459_bases
ATGAGCTCCGACATTTCCACCAACAAAAAAGCCCGCCGGGATTACGAAATTCTGGACACCTACGAATGCGGCATGGAACT
CAAGGGAACGGAAGTCAAATCCATCCGCGCCGGCAAGGTGAATATTGCCGATTCCTTCGCCAGGGTGGAAAACGGCCAGA
TGCTCCTGTACGGCTGCGACATCCAGCCCTGGGAGACAGCGGGAGAATTCTTCCAGCACCAGGCGCGCCGGCCCCGCCGC
CTTCTTCTTCATAAACGGGAAATTTTCAAACTGGAACAGCAGACCTCCCAGAAAGGCTGCTCCCTGGTAGCTCTCAAGCT
GTATTGGAAAAACGGCAAGGTGAAACTGGCTCTAGGCCTGGGCAAGGGCAAAACCCACCGCGACCAGCGCTACGATTTGA
AAGCCCGCGTGGAAATGCGCGAAGCCCAGCGGGAAGTGGCCCGCATCAACCGCCGTTAA

Upstream 100 bases:

>100_bases
TATTGCCGTTCCGGACCGGAATTCCGTACGGAATCCTCTCTATCCAGCCAGGCCCCGGTCTCCCAGCCCGGGGCCTTTTC
ATCCTTCTCCCCTTCGTGCC

Downstream 100 bases:

>100_bases
GCCAGGCGGCAACAGCATGGTTGCCGTCCGCTTCCTCCCGGAAGCCTTTCTCCGCCCCAGCCATTCCCTGATATCCGGCA
TCTGTCCGGGGATAAACGGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MSSDISTNKKARRDYEILDTYECGMELKGTEVKSIRAGKVNIADSFARVENGQMLLYGCDIQPWETAGEFFQHQARRPRR
LLLHKREIFKLEQQTSQKGCSLVALKLYWKNGKVKLALGLGKGKTHRDQRYDLKARVEMREAQREVARINRR

Sequences:

>Translated_152_residues
MSSDISTNKKARRDYEILDTYECGMELKGTEVKSIRAGKVNIADSFARVENGQMLLYGCDIQPWETAGEFFQHQARRPRR
LLLHKREIFKLEQQTSQKGCSLVALKLYWKNGKVKLALGLGKGKTHRDQRYDLKARVEMREAQREVARINRR
>Mature_151_residues
SSDISTNKKARRDYEILDTYECGMELKGTEVKSIRAGKVNIADSFARVENGQMLLYGCDIQPWETAGEFFQHQARRPRRL
LLHKREIFKLEQQTSQKGCSLVALKLYWKNGKVKLALGLGKGKTHRDQRYDLKARVEMREAQREVARINRR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=148, Percent_Identity=41.8918918918919, Blast_Score=118, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17701; Mature: 17570

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDISTNKKARRDYEILDTYECGMELKGTEVKSIRAGKVNIADSFARVENGQMLLYGCD
CCCCCCCCCHHHCCHHHHHHHHCCCEECCCEECEEECCEEEHHHHHHHCCCCCEEEEECC
IQPWETAGEFFQHQARRPRRLLLHKREIFKLEQQTSQKGCSLVALKLYWKNGKVKLALGL
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEEEEEC
GKGKTHRDQRYDLKARVEMREAQREVARINRR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSDISTNKKARRDYEILDTYECGMELKGTEVKSIRAGKVNIADSFARVENGQMLLYGCD
CCCCCCCCHHHCCHHHHHHHHCCCEECCCEECEEECCEEEHHHHHHHCCCCCEEEEECC
IQPWETAGEFFQHQARRPRRLLLHKREIFKLEQQTSQKGCSLVALKLYWKNGKVKLALGL
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEEEEEC
GKGKTHRDQRYDLKARVEMREAQREVARINRR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]