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The map label for this gene is gluQ [H]

Identifier: 187735982

GI number: 187735982

Start: 1782166

End: 1783011

Strand: Direct

Name: gluQ [H]

Synonym: Amuc_1491

Alternate gene names: 187735982

Gene position: 1782166-1783011 (Clockwise)

Preceding gene: 187735980

Following gene: 187735987

Centisome position: 66.9

GC content: 60.64

Gene sequence:

>846_bases
ATGGATTCCCCCATAATAACAAGGTTTGCTCCCAGCCCTACAGGCAATCTCCATCTGGGGCACGCCCTGGCTGCGTGGGA
AGCCCGTTCCCTGGCGAACCGTTTTTCCGGGAAGTGCGTGCTGAGGATGGAAGATATCGACCAGACGCGGTGCCGTCCGG
AGTTTGTTCAGGGCATATTGAAAGATCTGGAGTGGCTGGGCATCCGCTTTGACGGTCCCATGATGGTTCAGTCTTCACGC
TTCAGCGCTTATGAACACGCTCTTCAGACGCTGAAGGACCGGGGCGTGCTGTACCCCTGTTTCTGTACGCGCCGGGAGAT
TGCGGAGGAGGTGGCCGCCATGGGTGGGGCGCCGCAGGGGGGCTGCGTGGACGTATATCCGGGAACTTGCCGGCTGCTTG
ACAAGGGGCGCAGGAAAGAGCTTCTCAAATCCGGCAAACCTTTTTCCTGGCGGCTGGATTGCCGTGCTGCGGCGCGGATT
ACCGGTCCCCTGCTATGGAGGGACATGCGGTTCGGAGATCAGGTTTGCCGTCCAGAAGAACTGGGGGATGTGATTCTGGG
CCGCAAGGACTGCGCCGCCAGCTACCATATTGCCGTGGTTGTGGACGACGCTGCCCAGGGAGTTACCCATGTAAGCCGTG
GTGAGGATTTATTCCCTGTGACGGGCATCCACCGGACGCTCCAGGCCCTGTTGGAGCTTCCTGTTCCGCAGTGGTATCAT
CACCGGCTGGTGAAGGATGCTTCCGGCAAGCGGCTTGCCAAGAGGGACAGGAGCCTGAGCCTTCAGGAAATGCGTGCCGC
CGGGATGCGGCCGGAAGACGTGTTCCGGCTGATGCGGGAGAGCTGA

Upstream 100 bases:

>100_bases
CCAACTCTCCTACTTTCCCGGCTTCTATCTGTCAAGCCGCAATAATTTTCCGGAAAGGGTTTGAAAAGGTTCCTGCGCTT
TTTTATTGTTGGTGCGCCGC

Downstream 100 bases:

>100_bases
GGGATTTTCATTCCCTTATAGGCGGGGGTGCGTTGTTACGCCGGGAAAGGCAGGGGTCAGGCTGTGGCTTGCGTTTTTCC
AGCAGCCGGTCGGGTGATTT

Product: glutamyl-tRNA synthetase class Ic

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDSPIITRFAPSPTGNLHLGHALAAWEARSLANRFSGKCVLRMEDIDQTRCRPEFVQGILKDLEWLGIRFDGPMMVQSSR
FSAYEHALQTLKDRGVLYPCFCTRREIAEEVAAMGGAPQGGCVDVYPGTCRLLDKGRRKELLKSGKPFSWRLDCRAAARI
TGPLLWRDMRFGDQVCRPEELGDVILGRKDCAASYHIAVVVDDAAQGVTHVSRGEDLFPVTGIHRTLQALLELPVPQWYH
HRLVKDASGKRLAKRDRSLSLQEMRAAGMRPEDVFRLMRES

Sequences:

>Translated_281_residues
MDSPIITRFAPSPTGNLHLGHALAAWEARSLANRFSGKCVLRMEDIDQTRCRPEFVQGILKDLEWLGIRFDGPMMVQSSR
FSAYEHALQTLKDRGVLYPCFCTRREIAEEVAAMGGAPQGGCVDVYPGTCRLLDKGRRKELLKSGKPFSWRLDCRAAARI
TGPLLWRDMRFGDQVCRPEELGDVILGRKDCAASYHIAVVVDDAAQGVTHVSRGEDLFPVTGIHRTLQALLELPVPQWYH
HRLVKDASGKRLAKRDRSLSLQEMRAAGMRPEDVFRLMRES
>Mature_281_residues
MDSPIITRFAPSPTGNLHLGHALAAWEARSLANRFSGKCVLRMEDIDQTRCRPEFVQGILKDLEWLGIRFDGPMMVQSSR
FSAYEHALQTLKDRGVLYPCFCTRREIAEEVAAMGGAPQGGCVDVYPGTCRLLDKGRRKELLKSGKPFSWRLDCRAAARI
TGPLLWRDMRFGDQVCRPEELGDVILGRKDCAASYHIAVVVDDAAQGVTHVSRGEDLFPVTGIHRTLQALLELPVPQWYH
HRLVKDASGKRLAKRDRSLSLQEMRAAGMRPEDVFRLMRES

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=288, Percent_Identity=31.9444444444444, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788743, Length=277, Percent_Identity=32.129963898917, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=34.2105263157895, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786895, Length=107, Percent_Identity=31.7757009345794, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=282, Percent_Identity=28.7234042553192, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324540, Length=276, Percent_Identity=32.2463768115942, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321192, Length=91, Percent_Identity=37.3626373626374, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665321, Length=282, Percent_Identity=32.9787234042553, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 31631; Mature: 31631

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPIITRFAPSPTGNLHLGHALAAWEARSLANRFSGKCVLRMEDIDQTRCRPEFVQGIL
CCCCCEEEECCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCHHHHHHHH
KDLEWLGIRFDGPMMVQSSRFSAYEHALQTLKDRGVLYPCFCTRREIAEEVAAMGGAPQG
HHHHHHEEEECCCEEEECCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCC
GCVDVYPGTCRLLDKGRRKELLKSGKPFSWRLDCRAAARITGPLLWRDMRFGDQVCRPEE
CEEECCCCHHHHHCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHCCCHHH
LGDVILGRKDCAASYHIAVVVDDAAQGVTHVSRGEDLFPVTGIHRTLQALLELPVPQWYH
HHHHHHCCHHCCCCEEEEEEECCHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
HRLVKDASGKRLAKRDRSLSLQEMRAAGMRPEDVFRLMRES
HHHHHCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDSPIITRFAPSPTGNLHLGHALAAWEARSLANRFSGKCVLRMEDIDQTRCRPEFVQGIL
CCCCCEEEECCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCHHHHHHHH
KDLEWLGIRFDGPMMVQSSRFSAYEHALQTLKDRGVLYPCFCTRREIAEEVAAMGGAPQG
HHHHHHEEEECCCEEEECCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCC
GCVDVYPGTCRLLDKGRRKELLKSGKPFSWRLDCRAAARITGPLLWRDMRFGDQVCRPEE
CEEECCCCHHHHHCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHCCCHHH
LGDVILGRKDCAASYHIAVVVDDAAQGVTHVSRGEDLFPVTGIHRTLQALLELPVPQWYH
HHHHHHCCHHCCCCEEEEEEECCHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
HRLVKDASGKRLAKRDRSLSLQEMRAAGMRPEDVFRLMRES
HHHHHCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA