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The map label for this gene is pgk

Identifier: 187735910

GI number: 187735910

Start: 1696613

End: 1697824

Strand: Direct

Name: pgk

Synonym: Amuc_1418

Alternate gene names: 187735910

Gene position: 1696613-1697824 (Clockwise)

Preceding gene: 187735909

Following gene: 187735911

Centisome position: 63.68

GC content: 57.01

Gene sequence:

>1212_bases
ATGGCTAAACTTACTGTTCGCGACCTTGACGCCAAGGGAAAGGAAGTCCTCATGCGCGTGGATTTCAACGTCCCTCTCAA
GGATGGGGAAATTACCAATGACGCCCGCATTGTGGCTGCTCTCCCCACCATCAAGTTCCTGCTGGACCAGGGCGCACGCC
TCGTCCTTACCTCTCATTTGGGCCGTCCCAAAAACGAACCCGATCCCGCTTTTTCCCTGAAACCCGTTGCCGCCCGCCTT
TCCGAATTGCTCGGCAAGGACGTGAAATTCGTTTCCGCCGCCATTGGCCCGGAAGCGGAAGCCGCCCGCGCCGCCATGAA
GGACGGTGATGTGGTTCTGCTGGAAAATGTCCGTTTTTATCCTGGTGAAAAGAAGAACGATCCGGAGTTCGCCAAGTCCC
TGCTGGGCAATGCCACGCTGTTCGTGAACGACGCATTCGGCACGGCCCACCGTGCTCACGCTTCCACGGAAGGCGTGACG
CATTTTGCGGAAAAGAGCGCCATGGGCTTCCTGATTGAACGTGAACTGGAATACCTGGAAGGCAAGCTGGAAAACCCGGA
AAAACCCTTTGTGGTGATTATGGGCGGCGCCAAGGTGTCCGACAAGATTGAAGTGCTTTCCAAACTGATGGAGAAAGCTG
ATACGTTCCTGATTGGCGGCGCCATGGCCAATACGTTTCTGGCGGCTGAAGGGTATGACCTGGGCGCTTCCAAGATTGAA
GGAGACAAGCTGGATCTGGCCCGGGAAATTCTGGCGGCGGCCAAGGCCAAGGGCGTGAAATTCCTGCTTCCTGCAGATGT
GCGCGTAGCCATGAAATTTGAAGACGGTGCCGAAACCTTCTGCACGGCTCCTTTCGCTGAAGGCGGCAAGGTGCCGGAAG
GCGGCATGGCCATCGACATCGGCGACAAAGCCATTGAAGAGTTCTCCGCCATCATCAAGGATGCCAAGACCGTTCTGTGG
AACGGCCCGATGGGCGTATTTGAAATGGACTGCTTCGCCAAGGGAACCAAGGAAGTCGCGGAAGCCCTGGCGGATTCCAC
CGCCATTTCCATTGTAGGCGGCGGTGATTCCGTGACGGCAGCCAAGAAATTCAAGGTGCAGGATAAGCTTTCCTTCTGTT
CCACGGGCGGCGGCGCTTCCCTGGAACTGCTGGAAGGCAAGGTTCTTCCCGGTGTGGGGGCCTTGACCGACAAGTGCTGC
TGCGGCAAGTAA

Upstream 100 bases:

>100_bases
CGGCTTTCCTGGCCGGTTTTGCCGGGCGGGCGGGGATTTATCCGGGAGGGCAGAAATGCCCTCCCTTTTTTATTCATTGA
ACCAGACAATATAAACCGAT

Downstream 100 bases:

>100_bases
CCGTTCATGGCCGGGCGGATTGTTTTCGCCTGGCGTTCAGAATGGGCTTTTTCATTCCCCGCACCCGTTTTCGGGTGCGG
GATTTTTGATTTGCCGGCTT

Product: Phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAKLTVRDLDAKGKEVLMRVDFNVPLKDGEITNDARIVAALPTIKFLLDQGARLVLTSHLGRPKNEPDPAFSLKPVAARL
SELLGKDVKFVSAAIGPEAEAARAAMKDGDVVLLENVRFYPGEKKNDPEFAKSLLGNATLFVNDAFGTAHRAHASTEGVT
HFAEKSAMGFLIERELEYLEGKLENPEKPFVVIMGGAKVSDKIEVLSKLMEKADTFLIGGAMANTFLAAEGYDLGASKIE
GDKLDLAREILAAAKAKGVKFLLPADVRVAMKFEDGAETFCTAPFAEGGKVPEGGMAIDIGDKAIEEFSAIIKDAKTVLW
NGPMGVFEMDCFAKGTKEVAEALADSTAISIVGGGDSVTAAKKFKVQDKLSFCSTGGGASLELLEGKVLPGVGALTDKCC
CGK

Sequences:

>Translated_403_residues
MAKLTVRDLDAKGKEVLMRVDFNVPLKDGEITNDARIVAALPTIKFLLDQGARLVLTSHLGRPKNEPDPAFSLKPVAARL
SELLGKDVKFVSAAIGPEAEAARAAMKDGDVVLLENVRFYPGEKKNDPEFAKSLLGNATLFVNDAFGTAHRAHASTEGVT
HFAEKSAMGFLIERELEYLEGKLENPEKPFVVIMGGAKVSDKIEVLSKLMEKADTFLIGGAMANTFLAAEGYDLGASKIE
GDKLDLAREILAAAKAKGVKFLLPADVRVAMKFEDGAETFCTAPFAEGGKVPEGGMAIDIGDKAIEEFSAIIKDAKTVLW
NGPMGVFEMDCFAKGTKEVAEALADSTAISIVGGGDSVTAAKKFKVQDKLSFCSTGGGASLELLEGKVLPGVGALTDKCC
CGK
>Mature_402_residues
AKLTVRDLDAKGKEVLMRVDFNVPLKDGEITNDARIVAALPTIKFLLDQGARLVLTSHLGRPKNEPDPAFSLKPVAARLS
ELLGKDVKFVSAAIGPEAEAARAAMKDGDVVLLENVRFYPGEKKNDPEFAKSLLGNATLFVNDAFGTAHRAHASTEGVTH
FAEKSAMGFLIERELEYLEGKLENPEKPFVVIMGGAKVSDKIEVLSKLMEKADTFLIGGAMANTFLAAEGYDLGASKIEG
DKLDLAREILAAAKAKGVKFLLPADVRVAMKFEDGAETFCTAPFAEGGKVPEGGMAIDIGDKAIEEFSAIIKDAKTVLWN
GPMGVFEMDCFAKGTKEVAEALADSTAISIVGGGDSVTAAKKFKVQDKLSFCSTGGGASLELLEGKVLPGVGALTDKCCC
GK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=416, Percent_Identity=46.1538461538462, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=44.7115384615385, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=45.5, Blast_Score=326, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=49.1606714628297, Blast_Score=359, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319857, Length=416, Percent_Identity=46.6346153846154, Blast_Score=335, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=44.578313253012, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=44.9275362318841, Blast_Score=317, Evalue=9e-87,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_AKKM8 (B2UKW8)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878022.1
- ProteinModelPortal:   B2UKW8
- SMR:   B2UKW8
- GeneID:   6275714
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1418
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42693; Mature: 42561

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 202-202 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTVRDLDAKGKEVLMRVDFNVPLKDGEITNDARIVAALPTIKFLLDQGARLVLTSHL
CCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEHHC
GRPKNEPDPAFSLKPVAARLSELLGKDVKFVSAAIGPEAEAARAAMKDGDVVLLENVRFY
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEC
PGEKKNDPEFAKSLLGNATLFVNDAFGTAHRAHASTEGVTHFAEKSAMGFLIERELEYLE
CCCCCCCHHHHHHHHCCEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GKLENPEKPFVVIMGGAKVSDKIEVLSKLMEKADTFLIGGAMANTFLAAEGYDLGASKIE
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHEEEECCCCCCCCCCC
GDKLDLAREILAAAKAKGVKFLLPADVRVAMKFEDGAETFCTAPFAEGGKVPEGGMAIDI
CCHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCCCCEECCCCCCCCCCCCCCEEEEC
GDKAIEEFSAIIKDAKTVLWNGPMGVFEMDCFAKGTKEVAEALADSTAISIVGGGDSVTA
CHHHHHHHHHHHHHCCEEEECCCCCEEEHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
AKKFKVQDKLSFCSTGGGASLELLEGKVLPGVGALTDKCCCGK
HHHEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKLTVRDLDAKGKEVLMRVDFNVPLKDGEITNDARIVAALPTIKFLLDQGARLVLTSHL
CEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEHHC
GRPKNEPDPAFSLKPVAARLSELLGKDVKFVSAAIGPEAEAARAAMKDGDVVLLENVRFY
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEC
PGEKKNDPEFAKSLLGNATLFVNDAFGTAHRAHASTEGVTHFAEKSAMGFLIERELEYLE
CCCCCCCHHHHHHHHCCEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GKLENPEKPFVVIMGGAKVSDKIEVLSKLMEKADTFLIGGAMANTFLAAEGYDLGASKIE
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHEEEECCCCCCCCCCC
GDKLDLAREILAAAKAKGVKFLLPADVRVAMKFEDGAETFCTAPFAEGGKVPEGGMAIDI
CCHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCCCCEECCCCCCCCCCCCCCEEEEC
GDKAIEEFSAIIKDAKTVLWNGPMGVFEMDCFAKGTKEVAEALADSTAISIVGGGDSVTA
CHHHHHHHHHHHHHCCEEEECCCCCEEEHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
AKKFKVQDKLSFCSTGGGASLELLEGKVLPGVGALTDKCCCGK
HHHEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA