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The map label for this gene is gap [H]

Identifier: 187735909

GI number: 187735909

Start: 1695420

End: 1696445

Strand: Direct

Name: gap [H]

Synonym: Amuc_1417

Alternate gene names: 187735909

Gene position: 1695420-1696445 (Clockwise)

Preceding gene: 187735908

Following gene: 187735910

Centisome position: 63.64

GC content: 57.89

Gene sequence:

>1026_bases
ATGGCCAAATACGCTATTAACGGTTTTGGACGCATTGGTCGCAACGTACTGCGCGCCATGTCCAAGGAAGAACGCAACAA
GGTTGTTGCCATCAATGACCTGACTCCTATCGAAACGATCGCCCACCTGCTCAAGTATGACTCCACGCAGGGCAAGTTTG
ACGGTGAAATTTCCATCGAGGGTGATTATCTGGTCGTTGACGGTCACAAGATCCTCATCACCGTGGAACGTGATCCCGCC
AACCTTCCCTGGAAGGATCTGGGCGTGGACGTCGTTCTGGAATCCACCGGCCTGTTCACCAAGCGCGACGCCGCCAAGAA
GCACCTTGACGCCGGCGCCAAGAAGGTTCTTATTTCCGCTCCCTCCCCGGATCCGGACCTGACTTTCGTTCTGGGCATCA
ACGACAGCGAATACGATCCTGCCAAGCACGATATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTTGCTCCGATGGTG
AAGGTGCTGGACGACAAGTTCGGCGTTGAAAAGGGCATGATGAGCACGATTCACTCCTACACGAACGACCAGCGCATTCT
GGACCTTCCGCACAAGGATCCCCGCCGTGCCCGCGCCGCCGCGATCAACATCATTCCGACGACCACCGGCGCCGCCAAGG
CCATTGGTGAAGTAATGCCGAACCTGAAGGGTTCCCTGAACGGCGCTTCCTTCCGCGTTCCGACTCCGACCGGTTCCCTG
ACCGACTTTGTGGCCGTGCTCAAGAAGGATGTGACCGTGGAAGAAGTAAACGCCGCCATGAAGGAAGCCGCTGAAGGCCC
GCTGAAGGGCATTCTGGCTTACTCCGAAGAAGCGCTCGTTCTTCAGGACATCGTTTCCGACCCCCACTCCTGCATCTTTG
ACTCCGGCTTCACGTATGTGGTCGGCGGCAACCTGGTGAAGGTCTGCGGCTGGTACGACAACGAATGGGGTTACTCCAAC
CGCGCCGCCCAGGCCATGAAGAAGCTGGGCGACAGCCTGGGCTGCGGATGCTCCTGCGGCAAGTAA

Upstream 100 bases:

>100_bases
AGTGTTGACGGCTGGTTGAAACGTTTTTAAACTTGCAGCGTCCATGGTACTTGAATAAGTCCGGGACTAGTAATTAATCA
AATCTGAAGCCCTCAATCTT

Downstream 100 bases:

>100_bases
GTTCTTTCGGACAACGCTTGCTCTTCAGTTGCACGACAAGTCTTGCCGTCCGCCTGTTTCAATTTTCCGGCTTTCCTGGC
CGGTTTTGCCGGGCGGGCGG

Product: glyceraldehyde-3-phosphate dehydrogenase, type I

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAKYAINGFGRIGRNVLRAMSKEERNKVVAINDLTPIETIAHLLKYDSTQGKFDGEISIEGDYLVVDGHKILITVERDPA
NLPWKDLGVDVVLESTGLFTKRDAAKKHLDAGAKKVLISAPSPDPDLTFVLGINDSEYDPAKHDIVSNASCTTNCLAPMV
KVLDDKFGVEKGMMSTIHSYTNDQRILDLPHKDPRRARAAAINIIPTTTGAAKAIGEVMPNLKGSLNGASFRVPTPTGSL
TDFVAVLKKDVTVEEVNAAMKEAAEGPLKGILAYSEEALVLQDIVSDPHSCIFDSGFTYVVGGNLVKVCGWYDNEWGYSN
RAAQAMKKLGDSLGCGCSCGK

Sequences:

>Translated_341_residues
MAKYAINGFGRIGRNVLRAMSKEERNKVVAINDLTPIETIAHLLKYDSTQGKFDGEISIEGDYLVVDGHKILITVERDPA
NLPWKDLGVDVVLESTGLFTKRDAAKKHLDAGAKKVLISAPSPDPDLTFVLGINDSEYDPAKHDIVSNASCTTNCLAPMV
KVLDDKFGVEKGMMSTIHSYTNDQRILDLPHKDPRRARAAAINIIPTTTGAAKAIGEVMPNLKGSLNGASFRVPTPTGSL
TDFVAVLKKDVTVEEVNAAMKEAAEGPLKGILAYSEEALVLQDIVSDPHSCIFDSGFTYVVGGNLVKVCGWYDNEWGYSN
RAAQAMKKLGDSLGCGCSCGK
>Mature_340_residues
AKYAINGFGRIGRNVLRAMSKEERNKVVAINDLTPIETIAHLLKYDSTQGKFDGEISIEGDYLVVDGHKILITVERDPAN
LPWKDLGVDVVLESTGLFTKRDAAKKHLDAGAKKVLISAPSPDPDLTFVLGINDSEYDPAKHDIVSNASCTTNCLAPMVK
VLDDKFGVEKGMMSTIHSYTNDQRILDLPHKDPRRARAAAINIIPTTTGAAKAIGEVMPNLKGSLNGASFRVPTPTGSLT
DFVAVLKKDVTVEEVNAAMKEAAEGPLKGILAYSEEALVLQDIVSDPHSCIFDSGFTYVVGGNLVKVCGWYDNEWGYSNR
AAQAMKKLGDSLGCGCSCGK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=326, Percent_Identity=46.6257668711656, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI7669492, Length=329, Percent_Identity=46.8085106382979, Blast_Score=304, Evalue=7e-83,
Organism=Escherichia coli, GI1788079, Length=320, Percent_Identity=51.25, Blast_Score=334, Evalue=6e-93,
Organism=Escherichia coli, GI1789295, Length=323, Percent_Identity=46.4396284829721, Blast_Score=287, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17534677, Length=327, Percent_Identity=49.8470948012232, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17534679, Length=327, Percent_Identity=49.5412844036697, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32566163, Length=323, Percent_Identity=50.4643962848297, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17568413, Length=323, Percent_Identity=50.4643962848297, Blast_Score=321, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6322409, Length=323, Percent_Identity=50.4643962848297, Blast_Score=332, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6322468, Length=323, Percent_Identity=50.1547987616099, Blast_Score=331, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6321631, Length=323, Percent_Identity=50.1547987616099, Blast_Score=326, Evalue=3e-90,
Organism=Drosophila melanogaster, GI17933600, Length=331, Percent_Identity=49.5468277945619, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI18110149, Length=331, Percent_Identity=49.5468277945619, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=49.5468277945619, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=49.5468277945619, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=45.9876543209877, Blast_Score=279, Evalue=2e-75,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36743; Mature: 36612

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYAINGFGRIGRNVLRAMSKEERNKVVAINDLTPIETIAHLLKYDSTQGKFDGEISIE
CCCEEECHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEC
GDYLVVDGHKILITVERDPANLPWKDLGVDVVLESTGLFTKRDAAKKHLDAGAKKVLISA
CCEEEECCCEEEEEEECCCCCCCHHHCCEEEEEECCCCEEHHHHHHHHHCCCCCEEEEEC
PSPDPDLTFVLGINDSEYDPAKHDIVSNASCTTNCLAPMVKVLDDKFGVEKGMMSTIHSY
CCCCCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
TNDQRILDLPHKDPRRARAAAINIIPTTTGAAKAIGEVMPNLKGSLNGASFRVPTPTGSL
CCCCEEEECCCCCCHHHHHEEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHH
TDFVAVLKKDVTVEEVNAAMKEAAEGPLKGILAYSEEALVLQDIVSDPHSCIFDSGFTYV
HHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCEEECCCCEEE
VGGNLVKVCGWYDNEWGYSNRAAQAMKKLGDSLGCGCSCGK
ECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AKYAINGFGRIGRNVLRAMSKEERNKVVAINDLTPIETIAHLLKYDSTQGKFDGEISIE
CCEEECHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEC
GDYLVVDGHKILITVERDPANLPWKDLGVDVVLESTGLFTKRDAAKKHLDAGAKKVLISA
CCEEEECCCEEEEEEECCCCCCCHHHCCEEEEEECCCCEEHHHHHHHHHCCCCCEEEEEC
PSPDPDLTFVLGINDSEYDPAKHDIVSNASCTTNCLAPMVKVLDDKFGVEKGMMSTIHSY
CCCCCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
TNDQRILDLPHKDPRRARAAAINIIPTTTGAAKAIGEVMPNLKGSLNGASFRVPTPTGSL
CCCCEEEECCCCCCHHHHHEEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHH
TDFVAVLKKDVTVEEVNAAMKEAAEGPLKGILAYSEEALVLQDIVSDPHSCIFDSGFTYV
HHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCEEECCCCEEE
VGGNLVKVCGWYDNEWGYSNRAAQAMKKLGDSLGCGCSCGK
ECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]