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The map label for this gene is tcmK [H]

Identifier: 187735885

GI number: 187735885

Start: 1662676

End: 1663848

Strand: Direct

Name: tcmK [H]

Synonym: Amuc_1392

Alternate gene names: 187735885

Gene position: 1662676-1663848 (Clockwise)

Preceding gene: 187735883

Following gene: 187735886

Centisome position: 62.41

GC content: 61.3

Gene sequence:

>1173_bases
ATGGCGGGGGGTAAGAGTCCTGGAAGCCCGGAACCGCGGGTATGCGGCCTGGGCCTGGCTTCCGGCTTCGGCATGGGGGC
GGCAGTCCATCTTGCCGGGGTAAGGGAAGGGCGCAGAGTGTTGAAGCCGTTGAGCGAATTGTGGGGCGAAGGTAATCCCT
GGGGCGATGTATTGAGCGGATGGATTCCAGACAGGGAATTGTTATGCAGCCGCAGGAACGGCCCTGCTTCCCAGCTGGCG
CTGCTGTTGGCGCGCCAGGCCGTGGAGGATGCCGGATGGGGAAGGAAGGAATTGAGGGATGCCGCCCTGATTGTGGGCAG
TTCCCGCGGGAATGCTTCCGGATGGCTGAGCCCGTGGCCGGGACGGCGGCCCATGAAAATTTTGGCCGTTCCCAATTCCC
TGCATAGCGAACTTGCTTCCTGCGTCAGCATCGAATTGGGAATTTGCGGGCCTTATCACGTGCTGGCGAGCGGCTGTGCC
GCCGGCCTGGATGCCGTAGGCATGGCCGCCATGCTGATGCGGCAGGGAGTTGTTAAGAGAGCGCTGGCTATCGGTCTGGA
TTTGCCTTTGTGCCGGGAGCTGCTGGGGACGTACTGGGCCTCCGGCATGCTTTCCCGCAATGGTTTGAATGATCCCTACG
GCCCTCTGGCCGACGGCATGTGCATTTCCGAGGGAGGGGCGGCCATAGCCCTGGAATTGTCTTCTGCTCCAGGTATTTAT
GTGAAGGATTATCTGGTGAATTCCGATGCCTATAGCCCGCTGGGCATGCCGGAGGACGGAAAGAGCATAGCCGCCCTGCT
GGAGGCTGCTTTGAAAAGTCGGGATGGAGCGGTTCCTCTGACCGTTTGTCCCCATGCCAGCGGCACGGCGGGGAATAGCG
TGTCCGAACGGGCTGCTTTGAAAAGGGTTTTTAAAGACGGACTGCCGGATTTGAGGATGATGAAGCCGTGGACGGGGCAT
GCCATTGGAGGCAGCGGAATTTTGGAACTGGCCCTGATGCTGGTCTTTGCCAGGGAGGGGGTATTGCCTCCCAATCCTCC
CTGGGCCACCTTCCCGGAAGGCGGCGCCTGTTCTGCGGAGGAGTTGCCGTTGGCCGGGGGGCGGATGCTGGTCAAATCCG
CCGCTTCCATGGGCGGCCATAATGTGGTGCTGAGTCTGGCTGTGGACGGTTGA

Upstream 100 bases:

>100_bases
CTTCCATTGCAAGGTTGCAGCGGTGGTCTTTTCAGGTTGTTCCTGACGCATCGGAAGCTTGAGTTTTTCCGGGCAGGGCG
GTAGTCTGGCCGCCGTCATG

Downstream 100 bases:

>100_bases
ATATTGTTGAATAGTGAACGCTTATGAAGAATCCTGAACAGTTTTTGTCAGAGTTGAAGTCTGCGGGGCTGCTGCGCGTC
CTGCGGGATGTGGAATGCCT

Product: Beta-ketoacyl synthase

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAGGKSPGSPEPRVCGLGLASGFGMGAAVHLAGVREGRRVLKPLSELWGEGNPWGDVLSGWIPDRELLCSRRNGPASQLA
LLLARQAVEDAGWGRKELRDAALIVGSSRGNASGWLSPWPGRRPMKILAVPNSLHSELASCVSIELGICGPYHVLASGCA
AGLDAVGMAAMLMRQGVVKRALAIGLDLPLCRELLGTYWASGMLSRNGLNDPYGPLADGMCISEGGAAIALELSSAPGIY
VKDYLVNSDAYSPLGMPEDGKSIAALLEAALKSRDGAVPLTVCPHASGTAGNSVSERAALKRVFKDGLPDLRMMKPWTGH
AIGGSGILELALMLVFAREGVLPPNPPWATFPEGGACSAEELPLAGGRMLVKSAASMGGHNVVLSLAVDG

Sequences:

>Translated_390_residues
MAGGKSPGSPEPRVCGLGLASGFGMGAAVHLAGVREGRRVLKPLSELWGEGNPWGDVLSGWIPDRELLCSRRNGPASQLA
LLLARQAVEDAGWGRKELRDAALIVGSSRGNASGWLSPWPGRRPMKILAVPNSLHSELASCVSIELGICGPYHVLASGCA
AGLDAVGMAAMLMRQGVVKRALAIGLDLPLCRELLGTYWASGMLSRNGLNDPYGPLADGMCISEGGAAIALELSSAPGIY
VKDYLVNSDAYSPLGMPEDGKSIAALLEAALKSRDGAVPLTVCPHASGTAGNSVSERAALKRVFKDGLPDLRMMKPWTGH
AIGGSGILELALMLVFAREGVLPPNPPWATFPEGGACSAEELPLAGGRMLVKSAASMGGHNVVLSLAVDG
>Mature_389_residues
AGGKSPGSPEPRVCGLGLASGFGMGAAVHLAGVREGRRVLKPLSELWGEGNPWGDVLSGWIPDRELLCSRRNGPASQLAL
LLARQAVEDAGWGRKELRDAALIVGSSRGNASGWLSPWPGRRPMKILAVPNSLHSELASCVSIELGICGPYHVLASGCAA
GLDAVGMAAMLMRQGVVKRALAIGLDLPLCRELLGTYWASGMLSRNGLNDPYGPLADGMCISEGGAAIALELSSAPGIYV
KDYLVNSDAYSPLGMPEDGKSIAALLEAALKSRDGAVPLTVCPHASGTAGNSVSERAALKRVFKDGLPDLRMMKPWTGHA
IGGSGILELALMLVFAREGVLPPNPPWATFPEGGACSAEELPLAGGRMLVKSAASMGGHNVVLSLAVDG

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Specific For Elongation From C-10 To Unsaturated C-16 And C-18 Fatty Acids. [C]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Escherichia coli, GI1788663, Length=375, Percent_Identity=26.4, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI212642053, Length=288, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 40310; Mature: 40179

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGKSPGSPEPRVCGLGLASGFGMGAAVHLAGVREGRRVLKPLSELWGEGNPWGDVLSG
CCCCCCCCCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
WIPDRELLCSRRNGPASQLALLLARQAVEDAGWGRKELRDAALIVGSSRGNASGWLSPWP
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCC
GRRPMKILAVPNSLHSELASCVSIELGICGPYHVLASGCAAGLDAVGMAAMLMRQGVVKR
CCCCEEEEECCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAIGLDLPLCRELLGTYWASGMLSRNGLNDPYGPLADGMCISEGGAAIALELSSAPGIY
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCEE
VKDYLVNSDAYSPLGMPEDGKSIAALLEAALKSRDGAVPLTVCPHASGTAGNSVSERAAL
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHH
KRVFKDGLPDLRMMKPWTGHAIGGSGILELALMLVFAREGVLPPNPPWATFPEGGACSAE
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ELPLAGGRMLVKSAASMGGHNVVLSLAVDG
CCCCCCCHHHHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
AGGKSPGSPEPRVCGLGLASGFGMGAAVHLAGVREGRRVLKPLSELWGEGNPWGDVLSG
CCCCCCCCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
WIPDRELLCSRRNGPASQLALLLARQAVEDAGWGRKELRDAALIVGSSRGNASGWLSPWP
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCC
GRRPMKILAVPNSLHSELASCVSIELGICGPYHVLASGCAAGLDAVGMAAMLMRQGVVKR
CCCCEEEEECCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAIGLDLPLCRELLGTYWASGMLSRNGLNDPYGPLADGMCISEGGAAIALELSSAPGIY
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCEE
VKDYLVNSDAYSPLGMPEDGKSIAALLEAALKSRDGAVPLTVCPHASGTAGNSVSERAAL
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHH
KRVFKDGLPDLRMMKPWTGHAIGGSGILELALMLVFAREGVLPPNPPWATFPEGGACSAE
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ELPLAGGRMLVKSAASMGGHNVVLSLAVDG
CCCCCCCHHHHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2684656 [H]