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The map label for this gene is carA

Identifier: 187735744

GI number: 187735744

Start: 1500452

End: 1501570

Strand: Reverse

Name: carA

Synonym: Amuc_1250

Alternate gene names: 187735744

Gene position: 1501570-1500452 (Counterclockwise)

Preceding gene: 187735745

Following gene: 187735743

Centisome position: 56.36

GC content: 58.27

Gene sequence:

>1119_bases
ATGAGAGCCATTCTCGCACTGGAAGACGGAAGCGTCTTCATCGGTTCGCACTTCGGGGCCACGGGCACGGAAGTGGGGGA
AGCCTGCTTCAACACGTCCATGACCGGCTATCAGGAAGTATTGACGGATCCGTCCTACAGCGGCCAGATCGTCACCATGA
CTTATCCTCTGATCGGCAATTACGGCGTCAACCCGGAAGACGGGGAATCAGGCAAAGCGCAGGTCAGCGGCTTTGTGGTG
GCGGAACTGGCGAAAGTCCACTCCAACTGGCGTGCCACGGAATCCCTGGGCCCGTGGCTGGAAAAACAGGGCGTCATCGG
CATTGAAGGGGTGGATACCCGCAAAATCGCCAAGCATCTGCGCTCCGCCGGAGCCATGCGCGCCTGCCTCACCACGGAGC
TGACTCCGGAGGAAGCCGTGGAAGCAGCCAGAAACGCCCCCTCCATGGAAGGCTGCAATATCGTGGACAAAATCGGTTCC
CACCCCATGACACCGGAGGAAGTGGACGCGCTGGTTACGGGAAAAACTCTGCCCCCTGCGGAATACGACATCGTCGCCTT
TGACTTCGGCATTAAATACAACATCCTGCGCCAGTTGAGGGAAAACGGCTTCCGCGTAACCGTAGTTCCCGCCCACACGA
CGGCGGAGGAAGTGCTTGCCATGAACCCGGACGGCGTCTTTCTTTCCAATGGTCCGGGAGATCCCGCCACGCTGACGGAC
ATTCACCGGGAAGTAGCTGCATTGATTGGGAAAGTGCCCATGTTCGGCATCTGCCTGGGCCACCAGATTATTTCCCACGC
GCTGGGAGCGAAAACCTTCAAGCTGAAGTTCGGGCACCGTGGAGCGAACCATCCGGTCAAAGATTTGAAAACGGGTAAAA
TCAGCATTACGTCCCAGAACCACGGTTTTGCCGTGGATCCGGACACCGTGCCGGACGACGTGGAAATAACGCTGATCAAT
CTGAATGACAATACCGTGGAAGGCATCGCCCACAGGACGCTTCCCGTATTCAGCGTGCAATACCACCCGGAAGCGGCGCC
CGGCCCCAGAGACCCCCAGTATCTGTTCCGAGATTTCAGGAAAATGATTGCGGCCAGCAAGCGGCAACATGCCCGCTGA

Upstream 100 bases:

>100_bases
ATTGAGGCGCTGAAGAACAAAAATCTTCAGGTGTGCACTATTCAGGAGTACCACGCCCAAAACCTTTAATCCCGCACCAC
CTAAGCATACTTACATCACC

Downstream 100 bases:

>100_bases
CGAATCCGCCCCCTTCCTACGGACCAGAGCATTTTCCTCGCCTCGCTGGGAAAAATGCGTTATTTATGCCCGGAGCCCAT
GAATTGCGGCTCCGGGAGAG

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRAILALEDGSVFIGSHFGATGTEVGEACFNTSMTGYQEVLTDPSYSGQIVTMTYPLIGNYGVNPEDGESGKAQVSGFVV
AELAKVHSNWRATESLGPWLEKQGVIGIEGVDTRKIAKHLRSAGAMRACLTTELTPEEAVEAARNAPSMEGCNIVDKIGS
HPMTPEEVDALVTGKTLPPAEYDIVAFDFGIKYNILRQLRENGFRVTVVPAHTTAEEVLAMNPDGVFLSNGPGDPATLTD
IHREVAALIGKVPMFGICLGHQIISHALGAKTFKLKFGHRGANHPVKDLKTGKISITSQNHGFAVDPDTVPDDVEITLIN
LNDNTVEGIAHRTLPVFSVQYHPEAAPGPRDPQYLFRDFRKMIAASKRQHAR

Sequences:

>Translated_372_residues
MRAILALEDGSVFIGSHFGATGTEVGEACFNTSMTGYQEVLTDPSYSGQIVTMTYPLIGNYGVNPEDGESGKAQVSGFVV
AELAKVHSNWRATESLGPWLEKQGVIGIEGVDTRKIAKHLRSAGAMRACLTTELTPEEAVEAARNAPSMEGCNIVDKIGS
HPMTPEEVDALVTGKTLPPAEYDIVAFDFGIKYNILRQLRENGFRVTVVPAHTTAEEVLAMNPDGVFLSNGPGDPATLTD
IHREVAALIGKVPMFGICLGHQIISHALGAKTFKLKFGHRGANHPVKDLKTGKISITSQNHGFAVDPDTVPDDVEITLIN
LNDNTVEGIAHRTLPVFSVQYHPEAAPGPRDPQYLFRDFRKMIAASKRQHAR
>Mature_372_residues
MRAILALEDGSVFIGSHFGATGTEVGEACFNTSMTGYQEVLTDPSYSGQIVTMTYPLIGNYGVNPEDGESGKAQVSGFVV
AELAKVHSNWRATESLGPWLEKQGVIGIEGVDTRKIAKHLRSAGAMRACLTTELTPEEAVEAARNAPSMEGCNIVDKIGS
HPMTPEEVDALVTGKTLPPAEYDIVAFDFGIKYNILRQLRENGFRVTVVPAHTTAEEVLAMNPDGVFLSNGPGDPATLTD
IHREVAALIGKVPMFGICLGHQIISHALGAKTFKLKFGHRGANHPVKDLKTGKISITSQNHGFAVDPDTVPDDVEITLIN
LNDNTVEGIAHRTLPVFSVQYHPEAAPGPRDPQYLFRDFRKMIAASKRQHAR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=379, Percent_Identity=40.3693931398417, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI21361331, Length=376, Percent_Identity=36.968085106383, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI169790915, Length=376, Percent_Identity=36.968085106383, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=46.8253968253968, Blast_Score=340, Evalue=9e-95,
Organism=Escherichia coli, GI1789760, Length=151, Percent_Identity=27.1523178807947, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=381, Percent_Identity=42.7821522309711, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6322331, Length=398, Percent_Identity=38.4422110552764, Blast_Score=278, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6324878, Length=386, Percent_Identity=41.4507772020725, Blast_Score=262, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6322638, Length=163, Percent_Identity=28.8343558282209, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24642586, Length=379, Percent_Identity=40.1055408970976, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI45555749, Length=379, Percent_Identity=40.1055408970976, Blast_Score=254, Evalue=8e-68,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_AKKM8 (B2URJ0)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877856.1
- ProteinModelPortal:   B2URJ0
- SMR:   B2URJ0
- GeneID:   6274736
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1250
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40193; Mature: 40193

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 258-258 ACT_SITE 342-342 ACT_SITE 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAILALEDGSVFIGSHFGATGTEVGEACFNTSMTGYQEVLTDPSYSGQIVTMTYPLIGN
CCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECEECC
YGVNPEDGESGKAQVSGFVVAELAKVHSNWRATESLGPWLEKQGVIGIEGVDTRKIAKHL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHCCCEEECCCCHHHHHHHH
RSAGAMRACLTTELTPEEAVEAARNAPSMEGCNIVDKIGSHPMTPEEVDALVTGKTLPPA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCC
EYDIVAFDFGIKYNILRQLRENGFRVTVVPAHTTAEEVLAMNPDGVFLSNGPGDPATLTD
CEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCCEEEECCCCCCCHHHH
IHREVAALIGKVPMFGICLGHQIISHALGAKTFKLKFGHRGANHPVKDLKTGKISITSQN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCEEEEEECC
HGFAVDPDTVPDDVEITLINLNDNTVEGIAHRTLPVFSVQYHPEAAPGPRDPQYLFRDFR
CCEEECCCCCCCCEEEEEEECCCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHH
KMIAASKRQHAR
HHHHHHHHHCCC
>Mature Secondary Structure
MRAILALEDGSVFIGSHFGATGTEVGEACFNTSMTGYQEVLTDPSYSGQIVTMTYPLIGN
CCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECEECC
YGVNPEDGESGKAQVSGFVVAELAKVHSNWRATESLGPWLEKQGVIGIEGVDTRKIAKHL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHCCCEEECCCCHHHHHHHH
RSAGAMRACLTTELTPEEAVEAARNAPSMEGCNIVDKIGSHPMTPEEVDALVTGKTLPPA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCC
EYDIVAFDFGIKYNILRQLRENGFRVTVVPAHTTAEEVLAMNPDGVFLSNGPGDPATLTD
CEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHEEECCCCEEEECCCCCCCHHHH
IHREVAALIGKVPMFGICLGHQIISHALGAKTFKLKFGHRGANHPVKDLKTGKISITSQN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCEEEEEECC
HGFAVDPDTVPDDVEITLINLNDNTVEGIAHRTLPVFSVQYHPEAAPGPRDPQYLFRDFR
CCEEECCCCCCCCEEEEEEECCCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHH
KMIAASKRQHAR
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA