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The map label for this gene is gmd [H]

Identifier: 187735743

GI number: 187735743

Start: 1499232

End: 1500299

Strand: Reverse

Name: gmd [H]

Synonym: Amuc_1249

Alternate gene names: 187735743

Gene position: 1500299-1499232 (Counterclockwise)

Preceding gene: 187735744

Following gene: 187735742

Centisome position: 56.32

GC content: 55.15

Gene sequence:

>1068_bases
ATGAGCAAAATCGCATTAATCTCCGGCATCACGGGACAGGACGGTTCCTTTCTCGCAGAGTTCCTGATTGACAAAGGGTA
TGAAGTACACGGCATCCTGCGCCGGTCCTCCTCCTTCAACACGGGCCGCATCGAACACCTTTACCTGGACGAATGGGTGC
GGGACATGAAGAAAAACCGCCTGATTAACCTCCACTACGGGGACATGACGGATTCCAGTTCACTCATCCGCATCATCCAG
ACCGTGCAGCCGGATGAAATCTACAATCTGGCAGCCCAGAGCCATGTGAAAGTCAGCTTTGACGTACCGGAATACACGGC
GGAAACGGATGCCGTGGGAACCCTCCGCATGCTGGAAGCCGTCCGCATTCTGGGCATGGAAAAGAAATGCCGCATCTACC
AGGCTTCCACGTCCGAGCTTTTCGGGCTGGTGCAGGAAGTCCCCCAGAAGGAAACCACGCCGTTTTATCCCCGTTCACCC
TACGGCGTAGCCAAGCAATACGGATTCTGGATTACCAAGAATTACCGGGAATCTTACAATATGTTTGCCGTTAACGGCAT
CCTGTTCAACCACGAAAGCGAACGCCGCGGAGAAAACTTCGTTACCCGCAAAATCACGCTGGCGGCGGCACGCATTGCCC
AGGGCATGCAGGACAAGCTCTACCTGGGCAACCTGAACGCCCTGCGCGACTGGGGTTACGCCAAGGATTACGTGGAATGC
ATGTGGCTGATTCTCCAGCATGACACACCGGAGGATTTCGTCATCGCCACCGGGGAAATGCACACGGTCAGGGAATTCTG
CACGCTGGCCTTCCGTGAGGCCGGAATAGAGCTGGAATGGGAGGGAGAAGGCGTGGAAGAACGCGGACGCGACAGGAAGA
CGGGCAATATTCTGGTGGAAGTGGACCCCAAATATTTCCGCCCTGCCGAAGTTGAACAACTGTTGGGAGACCCAACCAAG
GCCAAAACCCTTCTTGGCTGGAACCCCACCTCCACTCCGTTTGAGGAACTCGTGCGCATCATGGTGCGCCACGACATGAA
ATACGTAAAAACTCCCGTTCATTTCTGA

Upstream 100 bases:

>100_bases
AAATGCGTTATTTATGCCCGGAGCCCATGAATTGCGGCTCCGGGAGAGGTTTCTTTTATTTCATCAACCTCTCCCGCCAG
CAAACAACCTGACACACCAT

Downstream 100 bases:

>100_bases
GATGAAAAAAAACAGCAGAATCTTTGTGGCCGGACACCACGGCCTCGTCGGTTCCGCCATCTGGAAATCCCTGGAAGACA
AAGGCTATTCCCACCTGATT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSKIALISGITGQDGSFLAEFLIDKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMKKNRLINLHYGDMTDSSSLIRIIQ
TVQPDEIYNLAAQSHVKVSFDVPEYTAETDAVGTLRMLEAVRILGMEKKCRIYQASTSELFGLVQEVPQKETTPFYPRSP
YGVAKQYGFWITKNYRESYNMFAVNGILFNHESERRGENFVTRKITLAAARIAQGMQDKLYLGNLNALRDWGYAKDYVEC
MWLILQHDTPEDFVIATGEMHTVREFCTLAFREAGIELEWEGEGVEERGRDRKTGNILVEVDPKYFRPAEVEQLLGDPTK
AKTLLGWNPTSTPFEELVRIMVRHDMKYVKTPVHF

Sequences:

>Translated_355_residues
MSKIALISGITGQDGSFLAEFLIDKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMKKNRLINLHYGDMTDSSSLIRIIQ
TVQPDEIYNLAAQSHVKVSFDVPEYTAETDAVGTLRMLEAVRILGMEKKCRIYQASTSELFGLVQEVPQKETTPFYPRSP
YGVAKQYGFWITKNYRESYNMFAVNGILFNHESERRGENFVTRKITLAAARIAQGMQDKLYLGNLNALRDWGYAKDYVEC
MWLILQHDTPEDFVIATGEMHTVREFCTLAFREAGIELEWEGEGVEERGRDRKTGNILVEVDPKYFRPAEVEQLLGDPTK
AKTLLGWNPTSTPFEELVRIMVRHDMKYVKTPVHF
>Mature_354_residues
SKIALISGITGQDGSFLAEFLIDKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMKKNRLINLHYGDMTDSSSLIRIIQT
VQPDEIYNLAAQSHVKVSFDVPEYTAETDAVGTLRMLEAVRILGMEKKCRIYQASTSELFGLVQEVPQKETTPFYPRSPY
GVAKQYGFWITKNYRESYNMFAVNGILFNHESERRGENFVTRKITLAAARIAQGMQDKLYLGNLNALRDWGYAKDYVECM
WLILQHDTPEDFVIATGEMHTVREFCTLAFREAGIELEWEGEGVEERGRDRKTGNILVEVDPKYFRPAEVEQLLGDPTKA
KTLLGWNPTSTPFEELVRIMVRHDMKYVKTPVHF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=62.3563218390805, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI1788366, Length=360, Percent_Identity=62.5, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI48994969, Length=185, Percent_Identity=30.2702702702703, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788353, Length=256, Percent_Identity=25.78125, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=349, Percent_Identity=64.4699140401146, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901790, Length=349, Percent_Identity=64.4699140401146, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901788, Length=349, Percent_Identity=64.4699140401146, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901786, Length=349, Percent_Identity=64.4699140401146, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539422, Length=349, Percent_Identity=64.4699140401146, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=64.8255813953488, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24158427, Length=352, Percent_Identity=61.9318181818182, Blast_Score=450, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 40860; Mature: 40729

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIALISGITGQDGSFLAEFLIDKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMKKNR
CCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHCC
LINLHYGDMTDSSSLIRIIQTVQPDEIYNLAAQSHVKVSFDVPEYTAETDAVGTLRMLEA
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHH
VRILGMEKKCRIYQASTSELFGLVQEVPQKETTPFYPRSPYGVAKQYGFWITKNYRESYN
HHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEEECHHHCCC
MFAVNGILFNHESERRGENFVTRKITLAAARIAQGMQDKLYLGNLNALRDWGYAKDYVEC
EEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHH
MWLILQHDTPEDFVIATGEMHTVREFCTLAFREAGIELEWEGEGVEERGRDRKTGNILVE
HHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCEEEE
VDPKYFRPAEVEQLLGDPTKAKTLLGWNPTSTPFEELVRIMVRHDMKYVKTPVHF
ECCCCCCCHHHHHHHCCCCHHCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKIALISGITGQDGSFLAEFLIDKGYEVHGILRRSSSFNTGRIEHLYLDEWVRDMKKNR
CCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHCC
LINLHYGDMTDSSSLIRIIQTVQPDEIYNLAAQSHVKVSFDVPEYTAETDAVGTLRMLEA
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHH
VRILGMEKKCRIYQASTSELFGLVQEVPQKETTPFYPRSPYGVAKQYGFWITKNYRESYN
HHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEEECHHHCCC
MFAVNGILFNHESERRGENFVTRKITLAAARIAQGMQDKLYLGNLNALRDWGYAKDYVEC
EEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHH
MWLILQHDTPEDFVIATGEMHTVREFCTLAFREAGIELEWEGEGVEERGRDRKTGNILVE
HHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCEEEE
VDPKYFRPAEVEQLLGDPTKAKTLLGWNPTSTPFEELVRIMVRHDMKYVKTPVHF
ECCCCCCCHHHHHHHCCCCHHCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]