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The map label for this gene is purH [H]

Identifier: 187735700

GI number: 187735700

Start: 1441659

End: 1443206

Strand: Direct

Name: purH [H]

Synonym: Amuc_1206

Alternate gene names: 187735700

Gene position: 1441659-1443206 (Clockwise)

Preceding gene: 187735695

Following gene: 187735701

Centisome position: 54.11

GC content: 59.24

Gene sequence:

>1548_bases
ATGGCTATTCAGCGCGCATTGATATCCGTTTCCGACAAGACCGGCCTGGAGGAGTTTGCCAAGGGCCTGCACGAGTTTGG
AGTAGAGCTTATTTCCACTGGCGGGACCGCCGCTTTCCTGAAAGGCCTGGGCCTTCCGGTGATTGAGATTTCCGACTATA
CCGGAGAACCGGAGTTATTTGAAGGGCGCCTGAAGACGCTTCATCCCATGGTGCACGGGGGCCTGCTGCACCGCCGCGAC
AATGAGGAGCATGTGCGCCAGGCTAAGGAAAACGGCATCAAGCCCATCGACCTGGTCTGCGTGAATCTGTATCCTTTTGA
AGAAACCGTCGCCAGGCCCGGAGTCACGCTGGAGGAAGCTATTGAAAAAATAGATATCGGCGGGCCGTCCATGCTCCGTT
CCGCCGCCAAGAATTACGCTTCCGTCACGGTGGTTTCGGATCCCGCGGATTACGCGCGCATTCTGGATGAAATGCAGACC
CACAAGGGGGATACGACCCTGAAGACCCGTGAAAACCTGGCGGTGAAGGTGTTTATGCGCACCTCCAATTACGATAACGC
CATCACTAATTATCTTGGCCACCAGAGCGCGGAAAGCACCAAGGGCAGCTTCTGCATCTGCGCTCCCCTGTATCAGGAAT
TGCGTTACGGGGACAATCCCCACCAGGAAGCCAGCCTGTACGGCAGCTTCGGGGATATTTTCCACCAGCTTCAGGGCAAG
GAGCTTTCCTATACGAACGTGCTGGACATTGAAGGGGCAGCCGAGCTGATTACCCAGTTCCGCCGCCCGACGGTGGGCAT
TTTGAAGCACACGAATCCCTGCGGCGTGGGCCAGGACGACGAAGACCTGCGCAACGCATGGCAGAAGGCTTTTGAAACGG
ATACGCAGGCCCCCTTCGGCGGCGTGATCGTGGTCAACCGCCCGATGACGGAAGGCCTGGCCCGTGTGCTGAGCGCCATT
TTCACGGATGTCATCATCGCTCCGGAGTATGATGCGGAAGCCCGCGCCATTCTCCAGAAGAAGAAAAATTGCCGCATCAT
CCGCATGAACACGGAAGCCTGGATGAAGGCGCGCCGCGAACCCATCATCCGTTCCGCGCCCGGCGGGTTCATGACCATGA
AGCGGGATACGGACGTGATGGGGCTGGACAATCTGGAAGCCAAGGTGGTGACCAAGCGCCCCCCGACCGAGGAAGAATTG
ACCGCCATGCGTTTCAACTGGCGTGTCGTGAAGCAGGTTCATTCCAACGCCATCGTTTTTGGCGGTACGGACCGCACGCT
TGGCATCGGCGCCGGGCAGATGAGCCGTGTGGATTCCGCCCGCATTGCCGTCTGGAAGGCCGGGCAGGCCGGCCTGGATC
TGAAGGGCAGCGTTGTGGCGTCCGACGCCATGTTCCCGTTTGCAGACGGCCTCCAGGTGGCGATTGACGCCGGAGCCACC
GCCTGCATCCAGCCCGGAGGTTCCATCCGCGACGAGGAAGTGATTGCCGCCGCTGATGCCGCGGGAATTGCCATGGTATT
TACGGGACACCGCCATTTCCTCCATTAA

Upstream 100 bases:

>100_bases
TTCACGCGGCAGTAGTATGTAGGGAAAGGAACCGCATTTATCGCGTTCTCTCCCGGAGGGGAGGGGGGCGCCCGCGGGTA
CATTACAGATATTTGATCTT

Downstream 100 bases:

>100_bases
TTTGAATGTTCGTCCCCGCCCCTTTGTCTTAAAACCGATATGCTGCTAGGTGTAAATATAGACCACATCGCCACGTTGAG
GCAGGCCCGCTACGCGACCA

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MAIQRALISVSDKTGLEEFAKGLHEFGVELISTGGTAAFLKGLGLPVIEISDYTGEPELFEGRLKTLHPMVHGGLLHRRD
NEEHVRQAKENGIKPIDLVCVNLYPFEETVARPGVTLEEAIEKIDIGGPSMLRSAAKNYASVTVVSDPADYARILDEMQT
HKGDTTLKTRENLAVKVFMRTSNYDNAITNYLGHQSAESTKGSFCICAPLYQELRYGDNPHQEASLYGSFGDIFHQLQGK
ELSYTNVLDIEGAAELITQFRRPTVGILKHTNPCGVGQDDEDLRNAWQKAFETDTQAPFGGVIVVNRPMTEGLARVLSAI
FTDVIIAPEYDAEARAILQKKKNCRIIRMNTEAWMKARREPIIRSAPGGFMTMKRDTDVMGLDNLEAKVVTKRPPTEEEL
TAMRFNWRVVKQVHSNAIVFGGTDRTLGIGAGQMSRVDSARIAVWKAGQAGLDLKGSVVASDAMFPFADGLQVAIDAGAT
ACIQPGGSIRDEEVIAAADAAGIAMVFTGHRHFLH

Sequences:

>Translated_515_residues
MAIQRALISVSDKTGLEEFAKGLHEFGVELISTGGTAAFLKGLGLPVIEISDYTGEPELFEGRLKTLHPMVHGGLLHRRD
NEEHVRQAKENGIKPIDLVCVNLYPFEETVARPGVTLEEAIEKIDIGGPSMLRSAAKNYASVTVVSDPADYARILDEMQT
HKGDTTLKTRENLAVKVFMRTSNYDNAITNYLGHQSAESTKGSFCICAPLYQELRYGDNPHQEASLYGSFGDIFHQLQGK
ELSYTNVLDIEGAAELITQFRRPTVGILKHTNPCGVGQDDEDLRNAWQKAFETDTQAPFGGVIVVNRPMTEGLARVLSAI
FTDVIIAPEYDAEARAILQKKKNCRIIRMNTEAWMKARREPIIRSAPGGFMTMKRDTDVMGLDNLEAKVVTKRPPTEEEL
TAMRFNWRVVKQVHSNAIVFGGTDRTLGIGAGQMSRVDSARIAVWKAGQAGLDLKGSVVASDAMFPFADGLQVAIDAGAT
ACIQPGGSIRDEEVIAAADAAGIAMVFTGHRHFLH
>Mature_514_residues
AIQRALISVSDKTGLEEFAKGLHEFGVELISTGGTAAFLKGLGLPVIEISDYTGEPELFEGRLKTLHPMVHGGLLHRRDN
EEHVRQAKENGIKPIDLVCVNLYPFEETVARPGVTLEEAIEKIDIGGPSMLRSAAKNYASVTVVSDPADYARILDEMQTH
KGDTTLKTRENLAVKVFMRTSNYDNAITNYLGHQSAESTKGSFCICAPLYQELRYGDNPHQEASLYGSFGDIFHQLQGKE
LSYTNVLDIEGAAELITQFRRPTVGILKHTNPCGVGQDDEDLRNAWQKAFETDTQAPFGGVIVVNRPMTEGLARVLSAIF
TDVIIAPEYDAEARAILQKKKNCRIIRMNTEAWMKARREPIIRSAPGGFMTMKRDTDVMGLDNLEAKVVTKRPPTEEELT
AMRFNWRVVKQVHSNAIVFGGTDRTLGIGAGQMSRVDSARIAVWKAGQAGLDLKGSVVASDAMFPFADGLQVAIDAGATA
CIQPGGSIRDEEVIAAADAAGIAMVFTGHRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=470, Percent_Identity=37.2340425531915, Blast_Score=259, Evalue=6e-69,
Organism=Escherichia coli, GI1790439, Length=533, Percent_Identity=47.8424015009381, Blast_Score=458, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71985564, Length=472, Percent_Identity=31.7796610169492, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71985574, Length=328, Percent_Identity=22.8658536585366, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=467, Percent_Identity=35.3319057815846, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323056, Length=466, Percent_Identity=34.3347639484979, Blast_Score=240, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24649832, Length=469, Percent_Identity=35.8208955223881, Blast_Score=251, Evalue=7e-67,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56358; Mature: 56227

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQRALISVSDKTGLEEFAKGLHEFGVELISTGGTAAFLKGLGLPVIEISDYTGEPELF
CCCHHHHHHCCCCCCHHHHHHHHHHHCHHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHH
EGRLKTLHPMVHGGLLHRRDNEEHVRQAKENGIKPIDLVCVNLYPFEETVARPGVTLEEA
HHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCCCCHHHH
IEKIDIGGPSMLRSAAKNYASVTVVSDPADYARILDEMQTHKGDTTLKTRENLAVKVFMR
HHHHCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEE
TSNYDNAITNYLGHQSAESTKGSFCICAPLYQELRYGDNPHQEASLYGSFGDIFHQLQGK
CCCCCHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCC
ELSYTNVLDIEGAAELITQFRRPTVGILKHTNPCGVGQDDEDLRNAWQKAFETDTQAPFG
CCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
GVIVVNRPMTEGLARVLSAIFTDVIIAPEYDAEARAILQKKKNCRIIRMNTEAWMKARRE
CEEEECCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHCC
PIIRSAPGGFMTMKRDTDVMGLDNLEAKVVTKRPPTEEELTAMRFNWRVVKQVHSNAIVF
CCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEE
GGTDRTLGIGAGQMSRVDSARIAVWKAGQAGLDLKGSVVASDAMFPFADGLQVAIDAGAT
CCCCCEEECCCCHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCEEEEECCCE
ACIQPGGSIRDEEVIAAADAAGIAMVFTGHRHFLH
EEECCCCCCCCCCEEEECCCCCEEEEEECCHHCCC
>Mature Secondary Structure 
AIQRALISVSDKTGLEEFAKGLHEFGVELISTGGTAAFLKGLGLPVIEISDYTGEPELF
CCHHHHHHCCCCCCHHHHHHHHHHHCHHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHH
EGRLKTLHPMVHGGLLHRRDNEEHVRQAKENGIKPIDLVCVNLYPFEETVARPGVTLEEA
HHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCCCCHHHH
IEKIDIGGPSMLRSAAKNYASVTVVSDPADYARILDEMQTHKGDTTLKTRENLAVKVFMR
HHHHCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEE
TSNYDNAITNYLGHQSAESTKGSFCICAPLYQELRYGDNPHQEASLYGSFGDIFHQLQGK
CCCCCHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCC
ELSYTNVLDIEGAAELITQFRRPTVGILKHTNPCGVGQDDEDLRNAWQKAFETDTQAPFG
CCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
GVIVVNRPMTEGLARVLSAIFTDVIIAPEYDAEARAILQKKKNCRIIRMNTEAWMKARRE
CEEEECCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHCC
PIIRSAPGGFMTMKRDTDVMGLDNLEAKVVTKRPPTEEELTAMRFNWRVVKQVHSNAIVF
CCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEE
GGTDRTLGIGAGQMSRVDSARIAVWKAGQAGLDLKGSVVASDAMFPFADGLQVAIDAGAT
CCCCCEEECCCCHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCEEEEECCCE
ACIQPGGSIRDEEVIAAADAAGIAMVFTGHRHFLH
EEECCCCCCCCCCEEEECCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA