Click here to switch to the map view.

The map label for this gene is thiH [H]

Identifier: 187735612

GI number: 187735612

Start: 1335469

End: 1336605

Strand: Direct

Name: thiH [H]

Synonym: Amuc_1117

Alternate gene names: 187735612

Gene position: 1335469-1336605 (Clockwise)

Preceding gene: 187735610

Following gene: 187735613

Centisome position: 50.13

GC content: 59.45

Gene sequence:

>1137_bases
ATGTCTTTTTCTGAAGAATTAAACGGTTTGATGGATTGCCCCACTCCCCTGGTACGCCGTTTCATGGCGTTGCTGGAACC
GGTTGACGACGCCCGCCTGGAGGAGATGGCGCAGGAGAGCCGGCGTCTGACGCGGCTGCATTTCGGCCGGACCATCCGCC
TGTTCGCCCCCATTTACCTGTCCAACGAATGCATCAATAATTGCAAGTACTGCGGTTTTTCCCGGGATAATCCCATTATC
CGCACCACGCTTACGGTGGATGAAGTGGTGCAGGAGGCCCGCTACCTGCACGGCCTGGGGCTGCGCAGCATCCTGCTGGT
GGCCGGGGAGCATCCCAAGTTCGTTTCCGACGGGTATATGCAGGAATGCCTGGACGCCCTGCATTCCTTTATTCCATCCC
TGGGGCTGGAGATAGGACCGTTGCCGGACGACCGTTATGCGGAGATCGTCCGCCACGGGGCGGAACAATTGGCCGTGTAT
CAGGAAACCTATAACCGGGAAGTGTATGAAACCCTGCATACGGCAGGGATGAAGAAAAATTTCAACTGGAGGCTGGACTG
CCCGGAACGCGCCTACCAGGGCGGTTTCCGCCGCATTCAGATAGGAGCCTTGTTCGGGCTTTCTCCGTGGCGGCGGGAGG
CCATGGCACTTGCCGTCCATCTGGATTACCTGCAGAAGCATTGCTGGAAATCCGCGCTTTCCGTGGCGTTTCCGCGCATG
CGTCCCTACGCCGGGAATTACGAGTATGAACCTGATCCGGACTTGATGCTGGATGACCGCCATTTTGTCCAGCTTATGGC
CGCCCTGCGCATCTGTTTCCCCAAGATAGGCATGTCCATCAGCACCCGTGAGCCCGCGCCGATGAGGAATGCCCTGATGC
ATTTGGGCATGACCCACATGTCCGCCATTGCGCGCACGGAACCGGGGGGCTACACGGGCGTGGGAACGGCTGCCGCCCAT
TTGACGGTGCGGGGCAACCGGGTGGATCTTCCCGATGGCCGGAAAGGGAATTGCAAGGCGACGGAGCAGTTTGAGATTTC
CGACCAGCGCACACCGGAGCAGGTGGTCGGAGCCATACGGAATGCCGGGCTGGAGCCTGTCTGGAAAGACTGGGATGCCG
CTCTGGATGTGGTGTAG

Upstream 100 bases:

>100_bases
TAAAAGATGGTATCTTTTGCAAGGGAGGTAGCAAATTGGGGTTTTCCGGTTCAGGAATGGAAATGCAGCCGGCTCTCTCA
AAATTTTACTTAGTTTACGT

Downstream 100 bases:

>100_bases
GAGAACCTTCTCCCGTCTCATTTCCTTGGAATGACATCCATATCACCGTGATGTTGTGATGGAGCATTGACGGCATGCTC
CGGCTGTCATATTTCCGGTA

Product: biotin and thiamin synthesis associated

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSFSEELNGLMDCPTPLVRRFMALLEPVDDARLEEMAQESRRLTRLHFGRTIRLFAPIYLSNECINNCKYCGFSRDNPII
RTTLTVDEVVQEARYLHGLGLRSILLVAGEHPKFVSDGYMQECLDALHSFIPSLGLEIGPLPDDRYAEIVRHGAEQLAVY
QETYNREVYETLHTAGMKKNFNWRLDCPERAYQGGFRRIQIGALFGLSPWRREAMALAVHLDYLQKHCWKSALSVAFPRM
RPYAGNYEYEPDPDLMLDDRHFVQLMAALRICFPKIGMSISTREPAPMRNALMHLGMTHMSAIARTEPGGYTGVGTAAAH
LTVRGNRVDLPDGRKGNCKATEQFEISDQRTPEQVVGAIRNAGLEPVWKDWDAALDVV

Sequences:

>Translated_378_residues
MSFSEELNGLMDCPTPLVRRFMALLEPVDDARLEEMAQESRRLTRLHFGRTIRLFAPIYLSNECINNCKYCGFSRDNPII
RTTLTVDEVVQEARYLHGLGLRSILLVAGEHPKFVSDGYMQECLDALHSFIPSLGLEIGPLPDDRYAEIVRHGAEQLAVY
QETYNREVYETLHTAGMKKNFNWRLDCPERAYQGGFRRIQIGALFGLSPWRREAMALAVHLDYLQKHCWKSALSVAFPRM
RPYAGNYEYEPDPDLMLDDRHFVQLMAALRICFPKIGMSISTREPAPMRNALMHLGMTHMSAIARTEPGGYTGVGTAAAH
LTVRGNRVDLPDGRKGNCKATEQFEISDQRTPEQVVGAIRNAGLEPVWKDWDAALDVV
>Mature_377_residues
SFSEELNGLMDCPTPLVRRFMALLEPVDDARLEEMAQESRRLTRLHFGRTIRLFAPIYLSNECINNCKYCGFSRDNPIIR
TTLTVDEVVQEARYLHGLGLRSILLVAGEHPKFVSDGYMQECLDALHSFIPSLGLEIGPLPDDRYAEIVRHGAEQLAVYQ
ETYNREVYETLHTAGMKKNFNWRLDCPERAYQGGFRRIQIGALFGLSPWRREAMALAVHLDYLQKHCWKSALSVAFPRMR
PYAGNYEYEPDPDLMLDDRHFVQLMAALRICFPKIGMSISTREPAPMRNALMHLGMTHMSAIARTEPGGYTGVGTAAAHL
TVRGNRVDLPDGRKGNCKATEQFEISDQRTPEQVVGAIRNAGLEPVWKDWDAALDVV

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=356, Percent_Identity=39.8876404494382, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42863; Mature: 42732

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEELNGLMDCPTPLVRRFMALLEPVDDARLEEMAQESRRLTRLHFGRTIRLFAPIYL
CCCHHHHCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHH
SNECINNCKYCGFSRDNPIIRTTLTVDEVVQEARYLHGLGLRSILLVAGEHPKFVSDGYM
CHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHCCHHH
QECLDALHSFIPSLGLEIGPLPDDRYAEIVRHGAEQLAVYQETYNREVYETLHTAGMKKN
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FNWRLDCPERAYQGGFRRIQIGALFGLSPWRREAMALAVHLDYLQKHCWKSALSVAFPRM
CCEEECCHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPYAGNYEYEPDPDLMLDDRHFVQLMAALRICFPKIGMSISTREPAPMRNALMHLGMTHM
CCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SAIARTEPGGYTGVGTAAAHLTVRGNRVDLPDGRKGNCKATEQFEISDQRTPEQVVGAIR
HHHHHCCCCCCCCCCHHEEEEEEECCEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHH
NAGLEPVWKDWDAALDVV
HCCCCCHHHCHHHHHCCC
>Mature Secondary Structure 
SFSEELNGLMDCPTPLVRRFMALLEPVDDARLEEMAQESRRLTRLHFGRTIRLFAPIYL
CCHHHHCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHH
SNECINNCKYCGFSRDNPIIRTTLTVDEVVQEARYLHGLGLRSILLVAGEHPKFVSDGYM
CHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHCCHHH
QECLDALHSFIPSLGLEIGPLPDDRYAEIVRHGAEQLAVYQETYNREVYETLHTAGMKKN
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FNWRLDCPERAYQGGFRRIQIGALFGLSPWRREAMALAVHLDYLQKHCWKSALSVAFPRM
CCEEECCHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPYAGNYEYEPDPDLMLDDRHFVQLMAALRICFPKIGMSISTREPAPMRNALMHLGMTHM
CCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SAIARTEPGGYTGVGTAAAHLTVRGNRVDLPDGRKGNCKATEQFEISDQRTPEQVVGAIR
HHHHHCCCCCCCCCCHHEEEEEEECCEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHH
NAGLEPVWKDWDAALDVV
HCCCCCHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]