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The map label for this gene is rho

Identifier: 187735591

GI number: 187735591

Start: 1308450

End: 1309874

Strand: Reverse

Name: rho

Synonym: Amuc_1096

Alternate gene names: 187735591

Gene position: 1309874-1308450 (Counterclockwise)

Preceding gene: 187735592

Following gene: 187735588

Centisome position: 49.17

GC content: 57.12

Gene sequence:

>1425_bases
ATGACTGAAGAACTCGACAACACCCCTTCCCCCGCCGGAGATATTCCGCAGGAAACCCTCCCCAAGCCCCTGCCCGCGCC
GGAAGAAACCGCCGGGGAACAGCCGGCTGCCGCTCCGGAGGAAAATAGAGGAAACGCAGTCCGGGAGGAGGAGGAAGCAG
CCCCTGTTCTGGAGCAGATTGACATCAATGAACTGCGGAAACGCCCCCTGAACGATCTTCAGGAAATGGCGGAAGGGCTG
CCCATCCGGAACGCGGCCTCCCTCACCAAGTCCCAGCTGGTTTTTGAATTGGGGAAACAGCTTCTGGCAAAGGGCCATGA
AGTCGTAGTTTCCGGGGTCATGGAACAAGCCAAGGATAATTACGCCATGCTGAGGGATCCGGTGAAAAGTTTCCGCACCT
CTCCGGATGATATTTATCTGGGCGGCAATCTCATCAAACCCCTGCATCTCCGCGTAGGCCAGCAGATCAAGGTCAGGCTG
CGCAAATTGCGGCCTCATGACAAGTACCTTTCTGCAGCCTCCGTCATCAGCGTGGAGGACATCCCTGCGGAAGACTACCG
GGCGCGCAGCGATTTTGAACGCCTCACCCCCCTCTTCCCCAAGGAACGCCTCCTTCTGGAAAACAAGGGGGTCAATTCCG
CCGCCATGCGCGTGCTGGACCTCATGACGCCCTTCGGCAAAGGGCAGCGCGGCCTGATTGTGGCCCCCCCGCGCGGAGGA
AAAACCGTTCTTCTGAAAACAATCGCCCGTTCCATCAGGGCCAATTATCCGGAAGTGGAACTGATTGTGCTGTTGCTGGA
CGAACGTCCGGAGGAAGTAACGGATTTTGAAGAAACCGTGGATGCTCCGGTATTCGCCTCCACTTTTGACGAACCTTCCC
GGCGCCATGCCCAGGTTTCCGATCTGGTTATCGAACGGGCCAAACGCCTGGTGGAAATGGGCAGAGACGTCGTCATCCTG
CTGGATTCCCTCACCAGGCTGGCCCGCGGCTACAATGCCAACCAGACGGGAGGACGCATCATGTCCGGCGGCCTGGGGTC
CAATGCATTGGAAAAACCGCGCAAATTCTTTTCCGCGGCGCGCAATGTGGAAGAAGGAGGCAGCCTGACCATCATCGCCA
CATGCCTGGTAGACACGGAATCAAGAATGGACGAAGTGATTTTTGAAGAATTCAAGGGAACGGGCAATCTGGAAATCCGC
CTGGACCGGGAACTTTCCGAACGGCGCATTTATCCGGCCATTTCCCTTTCCCAGAGCGGCACCCGCAATGACGACAGGCT
GTATAACGAACAGGAATTCGTCAAAATCATGCAATTGCGCCGCCAGCTCGCCATGAAACCGGGCTGGGAAGGCCTTCAGA
CTCTCCTGCAAAATATCTCCAAGACACAGAATAACGCGGAACTTCTGCTGACGGGGCTGCGGTAA

Upstream 100 bases:

>100_bases
ATCATGGAAAAAGTGGCTGCGGCCGACTTCGTCATCTGGAATGAAGGCCCCCCGGACCTGCTCCGCCAGCAAACCCAAAG
ACTTTACCAACATTTTTTTC

Downstream 100 bases:

>100_bases
GATTTTCTGCCCGGCCTGTTTTTCCCCCGTCCTCCGTTCCGCCCGCCGGAACGGAGGCTTTTTTATCAGGATATCTTCCG
CTGTTCCAAGATTCCGCGGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTEELDNTPSPAGDIPQETLPKPLPAPEETAGEQPAAAPEENRGNAVREEEEAAPVLEQIDINELRKRPLNDLQEMAEGL
PIRNAASLTKSQLVFELGKQLLAKGHEVVVSGVMEQAKDNYAMLRDPVKSFRTSPDDIYLGGNLIKPLHLRVGQQIKVRL
RKLRPHDKYLSAASVISVEDIPAEDYRARSDFERLTPLFPKERLLLENKGVNSAAMRVLDLMTPFGKGQRGLIVAPPRGG
KTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDVVIL
LDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIR
LDRELSERRIYPAISLSQSGTRNDDRLYNEQEFVKIMQLRRQLAMKPGWEGLQTLLQNISKTQNNAELLLTGLR

Sequences:

>Translated_474_residues
MTEELDNTPSPAGDIPQETLPKPLPAPEETAGEQPAAAPEENRGNAVREEEEAAPVLEQIDINELRKRPLNDLQEMAEGL
PIRNAASLTKSQLVFELGKQLLAKGHEVVVSGVMEQAKDNYAMLRDPVKSFRTSPDDIYLGGNLIKPLHLRVGQQIKVRL
RKLRPHDKYLSAASVISVEDIPAEDYRARSDFERLTPLFPKERLLLENKGVNSAAMRVLDLMTPFGKGQRGLIVAPPRGG
KTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDVVIL
LDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIR
LDRELSERRIYPAISLSQSGTRNDDRLYNEQEFVKIMQLRRQLAMKPGWEGLQTLLQNISKTQNNAELLLTGLR
>Mature_473_residues
TEELDNTPSPAGDIPQETLPKPLPAPEETAGEQPAAAPEENRGNAVREEEEAAPVLEQIDINELRKRPLNDLQEMAEGLP
IRNAASLTKSQLVFELGKQLLAKGHEVVVSGVMEQAKDNYAMLRDPVKSFRTSPDDIYLGGNLIKPLHLRVGQQIKVRLR
KLRPHDKYLSAASVISVEDIPAEDYRARSDFERLTPLFPKERLLLENKGVNSAAMRVLDLMTPFGKGQRGLIVAPPRGGK
TVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDVVILL
DSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIRL
DRELSERRIYPAISLSQSGTRNDDRLYNEQEFVKIMQLRRQLAMKPGWEGLQTLLQNISKTQNNAELLLTGLR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=413, Percent_Identity=44.3099273607748, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_AKKM8 (B2UR37)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877703.1
- GeneID:   6274005
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1096
- HOGENOM:   HBG335262
- OMA:   YNYLPGP
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF50249 Nucleic_acid_OB; SSF68912 Rho_N

EC number: NA

Molecular weight: Translated: 52946; Mature: 52815

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEELDNTPSPAGDIPQETLPKPLPAPEETAGEQPAAAPEENRGNAVREEEEAAPVLEQI
CCCCCCCCCCCCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
DINELRKRPLNDLQEMAEGLPIRNAASLTKSQLVFELGKQLLAKGHEVVVSGVMEQAKDN
CHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
YAMLRDPVKSFRTSPDDIYLGGNLIKPLHLRVGQQIKVRLRKLRPHDKYLSAASVISVED
HHHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHEEEECC
IPAEDYRARSDFERLTPLFPKERLLLENKGVNSAAMRVLDLMTPFGKGQRGLIVAPPRGG
CCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCC
KTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVS
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHH
DLVIERAKRLVEMGRDVVILLDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAA
HHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
RNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIRLDRELSERRIYPAISLSQSG
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCEEECCCCC
TRNDDRLYNEQEFVKIMQLRRQLAMKPGWEGLQTLLQNISKTQNNAELLLTGLR
CCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
TEELDNTPSPAGDIPQETLPKPLPAPEETAGEQPAAAPEENRGNAVREEEEAAPVLEQI
CCCCCCCCCCCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
DINELRKRPLNDLQEMAEGLPIRNAASLTKSQLVFELGKQLLAKGHEVVVSGVMEQAKDN
CHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
YAMLRDPVKSFRTSPDDIYLGGNLIKPLHLRVGQQIKVRLRKLRPHDKYLSAASVISVED
HHHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHEEEECC
IPAEDYRARSDFERLTPLFPKERLLLENKGVNSAAMRVLDLMTPFGKGQRGLIVAPPRGG
CCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCC
KTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVS
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHH
DLVIERAKRLVEMGRDVVILLDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAA
HHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
RNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIRLDRELSERRIYPAISLSQSG
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCEEECCCCC
TRNDDRLYNEQEFVKIMQLRRQLAMKPGWEGLQTLLQNISKTQNNAELLLTGLR
CCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA