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The map label for this gene is nadB1 [H]

Identifier: 187735574

GI number: 187735574

Start: 1289522

End: 1291126

Strand: Reverse

Name: nadB1 [H]

Synonym: Amuc_1079

Alternate gene names: 187735574

Gene position: 1291126-1289522 (Counterclockwise)

Preceding gene: 187735578

Following gene: 187735573

Centisome position: 48.46

GC content: 60.31

Gene sequence:

>1605_bases
ATGAAGACGAGCGACTTTTTAGTCATTGGGGCAGGTGTTGCCGGATTGAGTTTCGCCTTGAGAGCGGCGGAACACGGCAA
GGTTGCAGTCATTACAAAAGGCAAATTTCTGGATTGCAGTTCCGCCAAGGCACAGGGCGGCATCGCCGCCGTATGGGACC
GCAACGATTCCTTTGAAGACCATGTTGCGGATACGCTGGACGCCGGAGCGGGCCTCTGCAACGAACAGGCCGTGCGCACT
ATCGTGGAGGAAGGCCCGGACGCTATCCGGGAACTGCTGGAATGGGGAGTGAACTTTGACCCCGGCAACCAGGGAGAGGA
TTATGAACTGGCCAGGGAGGGCGGCCACACCAAACGGCGCATCCTCCACGCCAAAGACGCAACCGGACTGGAAATTACCT
CCAAACTTCTGGAGGCGGCCAAGCTTCACCCCAACATTGAACTGCTGGAAGACCATTTCGCCATTGACCTGATTACGACC
ACCAAGCTGGGCCTCGTCACGGAAGACCGCGTTCTGGGAGCCTACGTGCTGGACCGCGTCTCCGGGGAAGTGGAAATCTT
CCGGTCAGACCGCGTGCTGCTCGCCACCGGCGGCTGCGGGCGCGTGTATCTGTACACTACCAATAATGATTCCGCCACGG
GGGACGGCGTAGCCATGGCCTGGCGGGCGGGGGCCACCATCGCCAACATGGAATTCGTGCAGTTCCATCCCACCTGTTTC
TTCAACACCCGCGCGGAAGGCGCGGAAGCGCGCTCCTTCCTCATCTCCGAAGCCGTGCGCGGGGAAGGCGGCGTGCTGAT
AGGCGCGGACGGGAAGGAATTCATGCACAAGTACGACCCCCGCAAATCCCTGGCCCCGCGCGACATCACCGCCCGCGCCG
TAGATTCGGAAATGAAGAGGACGGGCAGCATGTGCGTTTATCTGGATATCTCCCACAAGCCGGAAGGGTTCGTCCAGGAA
CGTTTTCCCCTGATTTACGAGACATGCAACCGCTACGGCGTCAATCCCGCCAGGGAACCCATTCCCGTTGTTCCCGCAGC
GCATTTCCAGTGCGGCGGCGTGCTGACGGACGTCAACGGCCAGACCAGCCTGGACGGCCTTTACGCCGCAGGGGAAACCG
GCTGCACCGGTCTTCACGGCGCCAACCGGCTGGCTTCCAACTCCCTTCTGGAATGCGTGGTGGTCGCCAAGAGGGCGCTG
AACTCCATGCTCTCCCTGCATCCCCTGAGAAAGGATTCCCCCACTCCTTACGACATCCCAGCCTGGCACCACGGAGATAC
AGCCCTGCCTGACGAACTTTCCGTCATCTATCATAATTGGGATGAAATCCGCCGCCTGATGTGGGATTACGTCTCCATTG
TGCGGACCAATAAAAGGCTGGACCGCGCAGCCACGCGGCTCCGGATGCTGCACCGGGAGGTAAAGGACTTCTACTGGGGA
TACCGCATTACGCCGGACATCCTGGAATTGCGCAACCTGGTGGCGGTGGCCTCCCTGATCGTGGACTGCGCCATGCGCCG
CAATGAAAGCCGCGGCCTTCATTTCAATACGGATTATCCCGGCAAGCTGGACCACCGCTTCGTCACCCAACTGCACCGAT
GGTAA

Upstream 100 bases:

>100_bases
GCGTGTTTCACACTTCTTTTCCAGACAAATATGCCCTGCCGCCCCATCATTGGGCGTGACTTGGCAGCCCGCGTCATGGT
ACAATTCCGTACCATTCAAG

Downstream 100 bases:

>100_bases
ACTACCGGCAGCGTCAACAGGCATTACGGAAGGGGCTCTTCGGGAGCCCCCTCCTGTCCGCATGCCTGCTCACGCTGCCG
GTTCTGGTTTCCGCAGAAAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKTSDFLVIGAGVAGLSFALRAAEHGKVAVITKGKFLDCSSAKAQGGIAAVWDRNDSFEDHVADTLDAGAGLCNEQAVRT
IVEEGPDAIRELLEWGVNFDPGNQGEDYELAREGGHTKRRILHAKDATGLEITSKLLEAAKLHPNIELLEDHFAIDLITT
TKLGLVTEDRVLGAYVLDRVSGEVEIFRSDRVLLATGGCGRVYLYTTNNDSATGDGVAMAWRAGATIANMEFVQFHPTCF
FNTRAEGAEARSFLISEAVRGEGGVLIGADGKEFMHKYDPRKSLAPRDITARAVDSEMKRTGSMCVYLDISHKPEGFVQE
RFPLIYETCNRYGVNPAREPIPVVPAAHFQCGGVLTDVNGQTSLDGLYAAGETGCTGLHGANRLASNSLLECVVVAKRAL
NSMLSLHPLRKDSPTPYDIPAWHHGDTALPDELSVIYHNWDEIRRLMWDYVSIVRTNKRLDRAATRLRMLHREVKDFYWG
YRITPDILELRNLVAVASLIVDCAMRRNESRGLHFNTDYPGKLDHRFVTQLHRW

Sequences:

>Translated_534_residues
MKTSDFLVIGAGVAGLSFALRAAEHGKVAVITKGKFLDCSSAKAQGGIAAVWDRNDSFEDHVADTLDAGAGLCNEQAVRT
IVEEGPDAIRELLEWGVNFDPGNQGEDYELAREGGHTKRRILHAKDATGLEITSKLLEAAKLHPNIELLEDHFAIDLITT
TKLGLVTEDRVLGAYVLDRVSGEVEIFRSDRVLLATGGCGRVYLYTTNNDSATGDGVAMAWRAGATIANMEFVQFHPTCF
FNTRAEGAEARSFLISEAVRGEGGVLIGADGKEFMHKYDPRKSLAPRDITARAVDSEMKRTGSMCVYLDISHKPEGFVQE
RFPLIYETCNRYGVNPAREPIPVVPAAHFQCGGVLTDVNGQTSLDGLYAAGETGCTGLHGANRLASNSLLECVVVAKRAL
NSMLSLHPLRKDSPTPYDIPAWHHGDTALPDELSVIYHNWDEIRRLMWDYVSIVRTNKRLDRAATRLRMLHREVKDFYWG
YRITPDILELRNLVAVASLIVDCAMRRNESRGLHFNTDYPGKLDHRFVTQLHRW
>Mature_534_residues
MKTSDFLVIGAGVAGLSFALRAAEHGKVAVITKGKFLDCSSAKAQGGIAAVWDRNDSFEDHVADTLDAGAGLCNEQAVRT
IVEEGPDAIRELLEWGVNFDPGNQGEDYELAREGGHTKRRILHAKDATGLEITSKLLEAAKLHPNIELLEDHFAIDLITT
TKLGLVTEDRVLGAYVLDRVSGEVEIFRSDRVLLATGGCGRVYLYTTNNDSATGDGVAMAWRAGATIANMEFVQFHPTCF
FNTRAEGAEARSFLISEAVRGEGGVLIGADGKEFMHKYDPRKSLAPRDITARAVDSEMKRTGSMCVYLDISHKPEGFVQE
RFPLIYETCNRYGVNPAREPIPVVPAAHFQCGGVLTDVNGQTSLDGLYAAGETGCTGLHGANRLASNSLLECVVVAKRAL
NSMLSLHPLRKDSPTPYDIPAWHHGDTALPDELSVIYHNWDEIRRLMWDYVSIVRTNKRLDRAATRLRMLHREVKDFYWG
YRITPDILELRNLVAVASLIVDCAMRRNESRGLHFNTDYPGKLDHRFVTQLHRW

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=539, Percent_Identity=31.5398886827458, Blast_Score=212, Evalue=9e-55,
Organism=Escherichia coli, GI1788928, Length=526, Percent_Identity=51.3307984790874, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1790597, Length=537, Percent_Identity=32.4022346368715, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1786942, Length=568, Percent_Identity=30.6338028169014, Blast_Score=199, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17550100, Length=542, Percent_Identity=31.549815498155, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17505833, Length=541, Percent_Identity=30.8687615526802, Blast_Score=225, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI71986328, Length=269, Percent_Identity=31.2267657992565, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322701, Length=575, Percent_Identity=32.3478260869565, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322416, Length=558, Percent_Identity=31.0035842293907, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6320788, Length=481, Percent_Identity=25.7796257796258, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322511, Length=309, Percent_Identity=26.8608414239482, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17137288, Length=543, Percent_Identity=30.939226519337, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24655642, Length=543, Percent_Identity=30.939226519337, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24655647, Length=543, Percent_Identity=30.939226519337, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24663005, Length=565, Percent_Identity=29.9115044247788, Blast_Score=205, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59095; Mature: 59095

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSDFLVIGAGVAGLSFALRAAEHGKVAVITKGKFLDCSSAKAQGGIAAVWDRNDSFED
CCCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCCCCEEEEECCCCCHHH
HVADTLDAGAGLCNEQAVRTIVEEGPDAIRELLEWGVNFDPGNQGEDYELAREGGHTKRR
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHH
ILHAKDATGLEITSKLLEAAKLHPNIELLEDHFAIDLITTTKLGLVTEDRVLGAYVLDRV
EEECCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECEECEEECCHHHHHHHHHHC
SGEVEIFRSDRVLLATGGCGRVYLYTTNNDSATGDGVAMAWRAGATIANMEFVQFHPTCF
CCCEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECEEEEEECCEEE
FNTRAEGAEARSFLISEAVRGEGGVLIGADGKEFMHKYDPRKSLAPRDITARAVDSEMKR
ECCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
TGSMCVYLDISHKPEGFVQERFPLIYETCNRYGVNPAREPIPVVPAAHFQCGGVLTDVNG
CCCEEEEEEECCCCCCHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEECCC
QTSLDGLYAAGETGCTGLHGANRLASNSLLECVVVAKRALNSMLSLHPLRKDSPTPYDIP
CCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AWHHGDTALPDELSVIYHNWDEIRRLMWDYVSIVRTNKRLDRAATRLRMLHREVKDFYWG
CCCCCCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YRITPDILELRNLVAVASLIVDCAMRRNESRGLHFNTDYPGKLDHRFVTQLHRW
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKTSDFLVIGAGVAGLSFALRAAEHGKVAVITKGKFLDCSSAKAQGGIAAVWDRNDSFED
CCCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCCCCEEEEECCCCCHHH
HVADTLDAGAGLCNEQAVRTIVEEGPDAIRELLEWGVNFDPGNQGEDYELAREGGHTKRR
HHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHH
ILHAKDATGLEITSKLLEAAKLHPNIELLEDHFAIDLITTTKLGLVTEDRVLGAYVLDRV
EEECCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECEECEEECCHHHHHHHHHHC
SGEVEIFRSDRVLLATGGCGRVYLYTTNNDSATGDGVAMAWRAGATIANMEFVQFHPTCF
CCCEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECEEEEEECCEEE
FNTRAEGAEARSFLISEAVRGEGGVLIGADGKEFMHKYDPRKSLAPRDITARAVDSEMKR
ECCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
TGSMCVYLDISHKPEGFVQERFPLIYETCNRYGVNPAREPIPVVPAAHFQCGGVLTDVNG
CCCEEEEEEECCCCCCHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEECCC
QTSLDGLYAAGETGCTGLHGANRLASNSLLECVVVAKRALNSMLSLHPLRKDSPTPYDIP
CCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AWHHGDTALPDELSVIYHNWDEIRRLMWDYVSIVRTNKRLDRAATRLRMLHREVKDFYWG
CCCCCCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YRITPDILELRNLVAVASLIVDCAMRRNESRGLHFNTDYPGKLDHRFVTQLHRW
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]