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The map label for this gene is pcrA [H]

Identifier: 187735472

GI number: 187735472

Start: 1160384

End: 1162390

Strand: Reverse

Name: pcrA [H]

Synonym: Amuc_0971

Alternate gene names: 187735472

Gene position: 1162390-1160384 (Counterclockwise)

Preceding gene: 187735473

Following gene: 187735468

Centisome position: 43.63

GC content: 57.05

Gene sequence:

>2007_bases
ATGGCCAAGTCCTTTTCCATGGAGAGCCTGAACGCGGCCCAGAGGAAGGCCGTGCAGACCCTGCAGGGGCCCGTGCTGAT
CCTGGCCGGGGCCGGAACGGGTAAAACGCGCACCGTCACCTGCCGCATCGCCCATATGGTGGACCGTGGCATCAGCCCCA
AAAGCATCCTGGCCGTGACGTTCACCAACAAGGCCGCTCTGGAAATGCGGGAACGCGTAGGCCAGATGGTGGAACGCAAG
GCGGCGCGCCAAATAATGGTGAGCACTTTCCATTCCCTTTGCGTTCGTATCCTGAGAGAGGATATAGGACGCCTGGGGTA
CAAAACCAATTTCACCATTTACAGCGGTTCCGAGCAAAGCGGCCTGATCCGGCGACTGATTGTGCGCCACGGCGGCATTA
CGGAGAAAATCACGCCCAAAGATGTGCTTTCTTCCATGAGCCGGATGAAAAACAGCGGTCTGGGCCTTGACTCCATTGAA
GACAACCTGACAGCCAACATCGCCGCCTCCTACCAGCGGGAACTTCAGGCGCAGAATTCCGTGGACTTTGACGACCTGCT
GATTCTGGCGGACAAATTGCTGAAGGAGCACCGGGACGTCAGGGAGGCGTGGCAACAGCGCTTCCGCTATATCACCGTAG
ATGAATTCCAGGATACCAACAGCCTCCAAATGAGCCTGCTGGGCCATCTGGTAGGTCCGGAACACAATGTCTGCGTAGTG
GGAGACGACGACCAGTCCATCTACGGCTGGCGAGGCGCCCAGATCAGCAATATCCTGGAATTCGAACGTTTTTTCCCAAA
TCCCTCCGTCATCAAACTGGAAGAAAATTACCGCTGCACCGCCCCCATTCTGGACACGGCCAATGCCCTGATCCGCCACA
ATCTGGGCCGGAGGGACAAGACGCTGCGCGCCCACAAAGGCGGAGGAGAAGCCGTCCGGCTCATTTCCATGCCCGGAGAC
GCGGAAGAGGCGGAATTCATCATTACGGACATTGAAAACGTCCGCAGGCAGGAAGGCCGGCCCTGGGAGGATTTCGCCAT
TCTGTTCCGGGCCAATACCCAGAGCCGCATCATTGAACAAACGCTCCGGGAACACAAAATTCCCTACCGCATGGTGGGCG
CGCAAAGTTTTTTCGACCGCAAGGAGGTGAAAGACCTCATTTCCTATCTGGCGACTATCGAAAACCCGCAGGCTGACGAA
TACCTGCTGCGCATCCTCAACACGCCGCCGCGCGGCATCAGCGAGCTGACCAGCCATCTGGTCATCGACTGGAGCCGGGA
GCACGGCAACAGCGTCTGGGCAGCCCTCCAGGACGAAGAATTCCTGGCGGCCCTTTCCACCCGCGCCCGCAACAGCGTGC
AGGCATTCAATGAACTGATTGCAAAATACATTGACCTTTTCCAGGATAAGGAAACCGACTTCGGAGACATCCTGGAGCAG
CTTATTGAGGAAACCGGATTCAGCGAATACGTCACGCGCCTGTGCAAAACGGAAGCTGAAATCCAGAAACGGCTGGTGTC
CATCGGGGACGTGAAAGCCTCCCTCCGCAACTTCTGGCAACCCGGAAAAACCCTCCGGGACTACCTGGCCCAAGTCACCC
TGGACAAGGAGGATAATGATGACGACGTGGAAAACAAGCCAGGCGTATGCCTGATTACCATGCACGCCGCCAAGGGACTG
GAATTTCCGGTAGTGTACCTCGTGGGGCTGGAGGAAGGCATTCTTCCCCACAAGCGTTCCCTGGAAGACGGCAATTGCGA
CGAAGAACGCCGCCTGCTTTACGTAGGCATCACGCGCGCCCAGGAGAAACTCATGCTTACTTATTGCGCCACGCGCCTGC
GCTACGGGGACCGCATGCCTTGCCAGCGCTCCTCCTTCCTTAGTGAAATTCCTCCCCACCTAATGGAATATTACAAATGG
GAGGATCTCATGAACGCGGAAGCGACGGAAGAGGAATCCGGAAACTTTTTCGATTCCCTGCGCAGCATGCTTCTGGAAGA
GGAATAA

Upstream 100 bases:

>100_bases
GCTGGTGACAGACGTCCAGCTTGAAGGCAGCACGCGCATGAATATCTCCCAGCTCATTGCCGGCCACCCGAATTTGAAAG
ATATCCATTTTGACGTATAA

Downstream 100 bases:

>100_bases
GAGGCGGTATGGCGTTTTGCCGGCCTCTTTTCAACCATGGCCTTATTTTATCAGAGAACAAGGCGCCGTTTCCGTCCGGA
AACGCCAGTCTGCCGGCTTT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MAKSFSMESLNAAQRKAVQTLQGPVLILAGAGTGKTRTVTCRIAHMVDRGISPKSILAVTFTNKAALEMRERVGQMVERK
AARQIMVSTFHSLCVRILREDIGRLGYKTNFTIYSGSEQSGLIRRLIVRHGGITEKITPKDVLSSMSRMKNSGLGLDSIE
DNLTANIAASYQRELQAQNSVDFDDLLILADKLLKEHRDVREAWQQRFRYITVDEFQDTNSLQMSLLGHLVGPEHNVCVV
GDDDQSIYGWRGAQISNILEFERFFPNPSVIKLEENYRCTAPILDTANALIRHNLGRRDKTLRAHKGGGEAVRLISMPGD
AEEAEFIITDIENVRRQEGRPWEDFAILFRANTQSRIIEQTLREHKIPYRMVGAQSFFDRKEVKDLISYLATIENPQADE
YLLRILNTPPRGISELTSHLVIDWSREHGNSVWAALQDEEFLAALSTRARNSVQAFNELIAKYIDLFQDKETDFGDILEQ
LIEETGFSEYVTRLCKTEAEIQKRLVSIGDVKASLRNFWQPGKTLRDYLAQVTLDKEDNDDDVENKPGVCLITMHAAKGL
EFPVVYLVGLEEGILPHKRSLEDGNCDEERRLLYVGITRAQEKLMLTYCATRLRYGDRMPCQRSSFLSEIPPHLMEYYKW
EDLMNAEATEEESGNFFDSLRSMLLEEE

Sequences:

>Translated_668_residues
MAKSFSMESLNAAQRKAVQTLQGPVLILAGAGTGKTRTVTCRIAHMVDRGISPKSILAVTFTNKAALEMRERVGQMVERK
AARQIMVSTFHSLCVRILREDIGRLGYKTNFTIYSGSEQSGLIRRLIVRHGGITEKITPKDVLSSMSRMKNSGLGLDSIE
DNLTANIAASYQRELQAQNSVDFDDLLILADKLLKEHRDVREAWQQRFRYITVDEFQDTNSLQMSLLGHLVGPEHNVCVV
GDDDQSIYGWRGAQISNILEFERFFPNPSVIKLEENYRCTAPILDTANALIRHNLGRRDKTLRAHKGGGEAVRLISMPGD
AEEAEFIITDIENVRRQEGRPWEDFAILFRANTQSRIIEQTLREHKIPYRMVGAQSFFDRKEVKDLISYLATIENPQADE
YLLRILNTPPRGISELTSHLVIDWSREHGNSVWAALQDEEFLAALSTRARNSVQAFNELIAKYIDLFQDKETDFGDILEQ
LIEETGFSEYVTRLCKTEAEIQKRLVSIGDVKASLRNFWQPGKTLRDYLAQVTLDKEDNDDDVENKPGVCLITMHAAKGL
EFPVVYLVGLEEGILPHKRSLEDGNCDEERRLLYVGITRAQEKLMLTYCATRLRYGDRMPCQRSSFLSEIPPHLMEYYKW
EDLMNAEATEEESGNFFDSLRSMLLEEE
>Mature_667_residues
AKSFSMESLNAAQRKAVQTLQGPVLILAGAGTGKTRTVTCRIAHMVDRGISPKSILAVTFTNKAALEMRERVGQMVERKA
ARQIMVSTFHSLCVRILREDIGRLGYKTNFTIYSGSEQSGLIRRLIVRHGGITEKITPKDVLSSMSRMKNSGLGLDSIED
NLTANIAASYQRELQAQNSVDFDDLLILADKLLKEHRDVREAWQQRFRYITVDEFQDTNSLQMSLLGHLVGPEHNVCVVG
DDDQSIYGWRGAQISNILEFERFFPNPSVIKLEENYRCTAPILDTANALIRHNLGRRDKTLRAHKGGGEAVRLISMPGDA
EEAEFIITDIENVRRQEGRPWEDFAILFRANTQSRIIEQTLREHKIPYRMVGAQSFFDRKEVKDLISYLATIENPQADEY
LLRILNTPPRGISELTSHLVIDWSREHGNSVWAALQDEEFLAALSTRARNSVQAFNELIAKYIDLFQDKETDFGDILEQL
IEETGFSEYVTRLCKTEAEIQKRLVSIGDVKASLRNFWQPGKTLRDYLAQVTLDKEDNDDDVENKPGVCLITMHAAKGLE
FPVVYLVGLEEGILPHKRSLEDGNCDEERRLLYVGITRAQEKLMLTYCATRLRYGDRMPCQRSSFLSEIPPHLMEYYKWE
DLMNAEATEEESGNFFDSLRSMLLEEE

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=671, Percent_Identity=36.9597615499255, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI2367296, Length=653, Percent_Identity=36.7534456355283, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1787196, Length=361, Percent_Identity=27.9778393351801, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=732, Percent_Identity=27.8688524590164, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324477, Length=664, Percent_Identity=24.5481927710843, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76117; Mature: 75985

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSFSMESLNAAQRKAVQTLQGPVLILAGAGTGKTRTVTCRIAHMVDRGISPKSILAVT
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHHHHCCCCCCCEEEEE
FTNKAALEMRERVGQMVERKAARQIMVSTFHSLCVRILREDIGRLGYKTNFTIYSGSEQS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
GLIRRLIVRHGGITEKITPKDVLSSMSRMKNSGLGLDSIEDNLTANIAASYQRELQAQNS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHCCC
VDFDDLLILADKLLKEHRDVREAWQQRFRYITVDEFQDTNSLQMSLLGHLVGPEHNVCVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEE
GDDDQSIYGWRGAQISNILEFERFFPNPSVIKLEENYRCTAPILDTANALIRHNLGRRDK
CCCCCCEECCCCCHHHHHHHHHHHCCCCCEEEEECCCEECCCHHHHHHHHHHHCCCCCCC
TLRAHKGGGEAVRLISMPGDAEEAEFIITDIENVRRQEGRPWEDFAILFRANTQSRIIEQ
HHHHCCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHH
TLREHKIPYRMVGAQSFFDRKEVKDLISYLATIENPQADEYLLRILNTPPRGISELTSHL
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
VIDWSREHGNSVWAALQDEEFLAALSTRARNSVQAFNELIAKYIDLFQDKETDFGDILEQ
EEEECHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LIEETGFSEYVTRLCKTEAEIQKRLVSIGDVKASLRNFWQPGKTLRDYLAQVTLDKEDND
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCC
DDVENKPGVCLITMHAAKGLEFPVVYLVGLEEGILPHKRSLEDGNCDEERRLLYVGITRA
CCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHCEEEEEEEHHH
QEKLMLTYCATRLRYGDRMPCQRSSFLSEIPPHLMEYYKWEDLMNAEATEEESGNFFDSL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RSMLLEEE
HHHHHCCC
>Mature Secondary Structure 
AKSFSMESLNAAQRKAVQTLQGPVLILAGAGTGKTRTVTCRIAHMVDRGISPKSILAVT
CCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHHHHCCCCCCCEEEEE
FTNKAALEMRERVGQMVERKAARQIMVSTFHSLCVRILREDIGRLGYKTNFTIYSGSEQS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
GLIRRLIVRHGGITEKITPKDVLSSMSRMKNSGLGLDSIEDNLTANIAASYQRELQAQNS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHCCC
VDFDDLLILADKLLKEHRDVREAWQQRFRYITVDEFQDTNSLQMSLLGHLVGPEHNVCVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEE
GDDDQSIYGWRGAQISNILEFERFFPNPSVIKLEENYRCTAPILDTANALIRHNLGRRDK
CCCCCCEECCCCCHHHHHHHHHHHCCCCCEEEEECCCEECCCHHHHHHHHHHHCCCCCCC
TLRAHKGGGEAVRLISMPGDAEEAEFIITDIENVRRQEGRPWEDFAILFRANTQSRIIEQ
HHHHCCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHH
TLREHKIPYRMVGAQSFFDRKEVKDLISYLATIENPQADEYLLRILNTPPRGISELTSHL
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
VIDWSREHGNSVWAALQDEEFLAALSTRARNSVQAFNELIAKYIDLFQDKETDFGDILEQ
EEEECHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LIEETGFSEYVTRLCKTEAEIQKRLVSIGDVKASLRNFWQPGKTLRDYLAQVTLDKEDND
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCC
DDVENKPGVCLITMHAAKGLEFPVVYLVGLEEGILPHKRSLEDGNCDEERRLLYVGITRA
CCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHCEEEEEEEHHH
QEKLMLTYCATRLRYGDRMPCQRSSFLSEIPPHLMEYYKWEDLMNAEATEEESGNFFDSL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RSMLLEEE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]