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The map label for this gene is yfkN [H]

Identifier: 187735423

GI number: 187735423

Start: 1103658

End: 1105115

Strand: Reverse

Name: yfkN [H]

Synonym: Amuc_0922

Alternate gene names: 187735423

Gene position: 1105115-1103658 (Counterclockwise)

Preceding gene: 187735424

Following gene: 187735422

Centisome position: 41.48

GC content: 51.03

Gene sequence:

>1458_bases
ATGATTCCGCTCCGCATTGTTCTTGCCTCCCTGGCATGCCTGTACCCGTTCTCTCTGGCTCAGGAGCCTAAATCGGATCC
CGATAAAACGCTGATTATCATTTCTACCAGCGATATCCACGGCAATCTGGACAATTTCCCAAGACTGGCCACACTGGTAA
AGCAATACCGCGCTAAATATCCGCACGTGCTGCTGGTGGATTCAGGTGATTATTTCATAGGCAATCCCTATGTGGACGAC
TGTGAAAAACGGGGCGAACCCCTTACTATCCTGATGAACAAGCTGGGGTATGATGTAGTGACCATCGGAAACCATGATCT
GGATTACGGGCAGGAGGCCCTGCGCGACCATATCAAGGGAATGCCCTCCACCAAGTTTGTCATTACCAACGCCAGCCTTT
CCCCAACTCTGGAAAACTGTTTTTCTCCGTATGTCAGCATCCCGATTAAAGGAACGTCCATTTCCGTAGGGGTCATAGGA
CTGGCAGACCTTCAAACCACGGATGTGAGGAGGATGACCGGCATCTCATGGAAGCTGCCGGATGAAGAAGACTACAAGGG
AATCACGGACAGGTTCCGGCTCCACCATAATACCATCAACGTCATCCTCAGCCATCTGGGATACGGCAATGATCTGAAAA
TGATGAAGTATTCTCCGAATATCGACGTCATTCTTGGAGGCCATACCCATGTCATGCTGCCCAGCGGTCATCTCAGAACC
GGAACCCTGCTCAGCCACACGGGACACAGCCTGAGCCATGTAGGCGTTACGGAAATTATTTTCTCCACGGAACATCCCGT
CTCCATCCTTTCCAAATCAACCAGGGCCGTTTCTTTGAATGAGGAAATTCCCGATGACCCGGAAGTAAAGGAAATTGTCC
GCCGATTTTCCGGAAATCCGCTTTTCAACCAGCAAGTGGGCGTCGCCGGAGAAGAAATCACGCACGTCACCATAGGCACC
CTTTTCTGCAAGGCCATTCAGCAGGCCTCCCATTCGGATATTGCCATTTACAACCGCGGCGGAGTACGGTCCAAAAACCA
TCTTAACAAAGGACCCGTTACCATCAGGGACATTTACGAGCTGGAACCCTTCCGGGAAAAGATCGTCACCTGCTCCATGA
GTAAAGCGGACATTGAACAACTGATCCTGTCCAAATTCATGTCTCCGACCGATGACGAAGGAGGCGTTCTGGAAGTGTAC
TGCTCCGGATTCTCCTACCAGATCATGGACGGTGTTACGCCCAGCATTACCAGCACCTTGAAAAACGGGGTCATTTACAC
TGTCGCGATGGGAGACTACCTGTGCAGCAATTTCATATTCCCCCAACGGGGGAACGGCAAACCGACGGGAATAGATGTCC
GCAAAGCCCTGATTGATTACCTGAAACAAATCAAGGAGCTGCTCAATCCTCCGCCGTCCAAGCTACCTATCATCAAAGAT
ACGACGTTCGCCCTGTGA

Upstream 100 bases:

>100_bases
GGTCCGTCTGGAAGTCATCTCCGTGGGTGACGGCCCGCATGAGGTTTCCGCCCGTTAATCCCATCTCATCCTTTTCCGTA
TATTTATCATTTTACCCTGC

Downstream 100 bases:

>100_bases
ACTACGGCTGCAAGCACACGCTACATTCCCGCCAGCCATGCAGCCGGATCCCTATCATGCAGTACCGGAAGAGAAACGGA
ATATCAATGGTTTCCGCATC

Product: metallophosphoesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MIPLRIVLASLACLYPFSLAQEPKSDPDKTLIIISTSDIHGNLDNFPRLATLVKQYRAKYPHVLLVDSGDYFIGNPYVDD
CEKRGEPLTILMNKLGYDVVTIGNHDLDYGQEALRDHIKGMPSTKFVITNASLSPTLENCFSPYVSIPIKGTSISVGVIG
LADLQTTDVRRMTGISWKLPDEEDYKGITDRFRLHHNTINVILSHLGYGNDLKMMKYSPNIDVILGGHTHVMLPSGHLRT
GTLLSHTGHSLSHVGVTEIIFSTEHPVSILSKSTRAVSLNEEIPDDPEVKEIVRRFSGNPLFNQQVGVAGEEITHVTIGT
LFCKAIQQASHSDIAIYNRGGVRSKNHLNKGPVTIRDIYELEPFREKIVTCSMSKADIEQLILSKFMSPTDDEGGVLEVY
CSGFSYQIMDGVTPSITSTLKNGVIYTVAMGDYLCSNFIFPQRGNGKPTGIDVRKALIDYLKQIKELLNPPPSKLPIIKD
TTFAL

Sequences:

>Translated_485_residues
MIPLRIVLASLACLYPFSLAQEPKSDPDKTLIIISTSDIHGNLDNFPRLATLVKQYRAKYPHVLLVDSGDYFIGNPYVDD
CEKRGEPLTILMNKLGYDVVTIGNHDLDYGQEALRDHIKGMPSTKFVITNASLSPTLENCFSPYVSIPIKGTSISVGVIG
LADLQTTDVRRMTGISWKLPDEEDYKGITDRFRLHHNTINVILSHLGYGNDLKMMKYSPNIDVILGGHTHVMLPSGHLRT
GTLLSHTGHSLSHVGVTEIIFSTEHPVSILSKSTRAVSLNEEIPDDPEVKEIVRRFSGNPLFNQQVGVAGEEITHVTIGT
LFCKAIQQASHSDIAIYNRGGVRSKNHLNKGPVTIRDIYELEPFREKIVTCSMSKADIEQLILSKFMSPTDDEGGVLEVY
CSGFSYQIMDGVTPSITSTLKNGVIYTVAMGDYLCSNFIFPQRGNGKPTGIDVRKALIDYLKQIKELLNPPPSKLPIIKD
TTFAL
>Mature_485_residues
MIPLRIVLASLACLYPFSLAQEPKSDPDKTLIIISTSDIHGNLDNFPRLATLVKQYRAKYPHVLLVDSGDYFIGNPYVDD
CEKRGEPLTILMNKLGYDVVTIGNHDLDYGQEALRDHIKGMPSTKFVITNASLSPTLENCFSPYVSIPIKGTSISVGVIG
LADLQTTDVRRMTGISWKLPDEEDYKGITDRFRLHHNTINVILSHLGYGNDLKMMKYSPNIDVILGGHTHVMLPSGHLRT
GTLLSHTGHSLSHVGVTEIIFSTEHPVSILSKSTRAVSLNEEIPDDPEVKEIVRRFSGNPLFNQQVGVAGEEITHVTIGT
LFCKAIQQASHSDIAIYNRGGVRSKNHLNKGPVTIRDIYELEPFREKIVTCSMSKADIEQLILSKFMSPTDDEGGVLEVY
CSGFSYQIMDGVTPSITSTLKNGVIYTVAMGDYLCSNFIFPQRGNGKPTGIDVRKALIDYLKQIKELLNPPPSKLPIIKD
TTFAL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=428, Percent_Identity=25, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1790658, Length=243, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652512, Length=373, Percent_Identity=24.3967828418231, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921980, Length=373, Percent_Identity=24.3967828418231, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI161076508, Length=373, Percent_Identity=24.3967828418231, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24641187, Length=396, Percent_Identity=23.2323232323232, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922446, Length=469, Percent_Identity=22.3880597014925, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24654424, Length=469, Percent_Identity=22.3880597014925, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221329836, Length=382, Percent_Identity=22.7748691099476, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 53656; Mature: 53656

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLRIVLASLACLYPFSLAQEPKSDPDKTLIIISTSDIHGNLDNFPRLATLVKQYRAKY
CCHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
PHVLLVDSGDYFIGNPYVDDCEKRGEPLTILMNKLGYDVVTIGNHDLDYGQEALRDHIKG
CEEEEEECCCEEECCCCHHHHHHCCCCEEEEEHHHCCEEEEECCCCCHHHHHHHHHHHCC
MPSTKFVITNASLSPTLENCFSPYVSIPIKGTSISVGVIGLADLQTTDVRRMTGISWKLP
CCCCEEEEECCCCCHHHHHHCCCCEEEEECCCEEEEEEEEEECCCHHHHHHHCCCEEECC
DEEDYKGITDRFRLHHNTINVILSHLGYGNDLKMMKYSPNIDVILGGHTHVMLPSGHLRT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCEEEEECCCCCCC
GTLLSHTGHSLSHVGVTEIIFSTEHPVSILSKSTRAVSLNEEIPDDPEVKEIVRRFSGNP
CEEECCCCCCCHHCCHHHEEECCCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCC
LFNQQVGVAGEEITHVTIGTLFCKAIQQASHSDIAIYNRGGVRSKNHLNKGPVTIRDIYE
CCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEHHHH
LEPFREKIVTCSMSKADIEQLILSKFMSPTDDEGGVLEVYCSGFSYQIMDGVTPSITSTL
CCHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHH
KNGVIYTVAMGDYLCSNFIFPQRGNGKPTGIDVRKALIDYLKQIKELLNPPPSKLPIIKD
HCCEEEEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
TTFAL
CCCCC
>Mature Secondary Structure
MIPLRIVLASLACLYPFSLAQEPKSDPDKTLIIISTSDIHGNLDNFPRLATLVKQYRAKY
CCHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
PHVLLVDSGDYFIGNPYVDDCEKRGEPLTILMNKLGYDVVTIGNHDLDYGQEALRDHIKG
CEEEEEECCCEEECCCCHHHHHHCCCCEEEEEHHHCCEEEEECCCCCHHHHHHHHHHHCC
MPSTKFVITNASLSPTLENCFSPYVSIPIKGTSISVGVIGLADLQTTDVRRMTGISWKLP
CCCCEEEEECCCCCHHHHHHCCCCEEEEECCCEEEEEEEEEECCCHHHHHHHCCCEEECC
DEEDYKGITDRFRLHHNTINVILSHLGYGNDLKMMKYSPNIDVILGGHTHVMLPSGHLRT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCEEEEECCCCCCC
GTLLSHTGHSLSHVGVTEIIFSTEHPVSILSKSTRAVSLNEEIPDDPEVKEIVRRFSGNP
CEEECCCCCCCHHCCHHHEEECCCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCC
LFNQQVGVAGEEITHVTIGTLFCKAIQQASHSDIAIYNRGGVRSKNHLNKGPVTIRDIYE
CCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEHHHH
LEPFREKIVTCSMSKADIEQLILSKFMSPTDDEGGVLEVYCSGFSYQIMDGVTPSITSTL
CCHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHH
KNGVIYTVAMGDYLCSNFIFPQRGNGKPTGIDVRKALIDYLKQIKELLNPPPSKLPIIKD
HCCEEEEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
TTFAL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]