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The map label for this gene is ygcA [C]

Identifier: 187735352

GI number: 187735352

Start: 1013808

End: 1015031

Strand: Reverse

Name: ygcA [C]

Synonym: Amuc_0850

Alternate gene names: 187735352

Gene position: 1015031-1013808 (Counterclockwise)

Preceding gene: 187735353

Following gene: 187735351

Centisome position: 38.1

GC content: 58.42

Gene sequence:

>1224_bases
ATGACTGCGACCATTCCCAAGGGTTTCCACCCCGAACCTTTTTCCTACCACCAAGAGCTTGAACTGGATATTGACGCCCT
TTCCAATGCAGGCGACGGCATCGGCCGCGTGGACGGATGGGTGGTTTTCGTCCCCTTCGCCCTGCCGGGAGACCGTGTAA
AGGCGCGCGTCTGGCGTAATGACAGGAACTATTCCTCCGCCGATCTGGTGGAAGTAATTAACCCCAGCCCGGACCGCGTG
GAACCGGGCTGCCGCCTGTTCGGAACCTGTGGGGGATGCCAGTACCAGCATTTTTCCTATGACCGCCAGCTTCTCTGGAA
GACCCGGCAGGTAGCGGATCTGCTCCGCTTGCAGGCAGGGCTGGAACTGCCCGTCAATCCGGCCGTAGCCTCCCCCCGCC
AATACCATTACCGCTCCAAGATTACGCCCCATTTTGACAGGCCGAAGGAAGGAGGCAAGCCGGCCATCGGTTTCCTGAAA
GCAGGCTCCAGAAGGGACGTAGTGGACGTGCCGCAATGCCCGATTGCCATGGAGTGCATCAATGAAGCCCTGCCCCTGGC
CCGCAAAAGCGTTTACCAGGCCGCAGCCCGGTTCAAGCGGGGAGCCACCATTCTGCTCCGGGCCTCGGAAGGAACTGTCA
TCACCAATAACAATGCCGTCGCCTGCGAACGGGTGGGCGATTTGGAATTCCATTTTCTGGCGGGAGACTTTTTCCAGAAT
AATCCTTTCATTCTTCCGCTTTTTACGGATTACGTGGCCCAACAGGCGAGTATGGACGGGGAGGAATTCCTGGTGGACGC
CTACTGCGGTTCCGGCCTGTTCGCCCTGAGCCTGGCGAAGAAATTTAAAAAAGTGCTGGGCGTGGAAGTCAGCGAGACTT
CCGCGGACTGGGCACGCAGCAATGCCCGCAGCAACGGAATTGAACACGCGGAATTCCTGGCGGCGGACGCCGGAGCCATT
TTTGCCCGGGTGGATTTCCCCGCGGAAAAAACCGCCGTGGTGATCGACCCTCCCCGGAAAGGATGCAGTGCGGAATTTTT
GGCCCAGTTATTTGCCTTCGGCCCCGGAAAAGTCGTTTACGTTTCCTGCAATCCCGCCACCCAGATACGGGATCTGGCGG
AATTCGACAAGGCCGGGTATTTCGTCACCGCCGTCCAGCCCTTTGACCTGTTCCCCCAGACCAAGCATCTGGAATGCGTG
GTCACCCTGAAAAAAAATACCTGA

Upstream 100 bases:

>100_bases
CCGCCCCGGACTGCGCACCCCCCGGCTGCCGGAAACCCTGCTGTACGATCTGGACGGCAAGTTGATTACCCCTTCCGCCC
CATAATCTTTTCTCACCACC

Downstream 100 bases:

>100_bases
TTTTTTCCATGCCCAAGCTTTATATCAAAACCTACGGCTGCCAGATGAACGAACGGGACTCCGAACAGGTGGCCCGCATG
TTCGTGCAGAAGGGCTACAC

Product: RNA methyltransferase, TrmA family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTATIPKGFHPEPFSYHQELELDIDALSNAGDGIGRVDGWVVFVPFALPGDRVKARVWRNDRNYSSADLVEVINPSPDRV
EPGCRLFGTCGGCQYQHFSYDRQLLWKTRQVADLLRLQAGLELPVNPAVASPRQYHYRSKITPHFDRPKEGGKPAIGFLK
AGSRRDVVDVPQCPIAMECINEALPLARKSVYQAAARFKRGATILLRASEGTVITNNNAVACERVGDLEFHFLAGDFFQN
NPFILPLFTDYVAQQASMDGEEFLVDAYCGSGLFALSLAKKFKKVLGVEVSETSADWARSNARSNGIEHAEFLAADAGAI
FARVDFPAEKTAVVIDPPRKGCSAEFLAQLFAFGPGKVVYVSCNPATQIRDLAEFDKAGYFVTAVQPFDLFPQTKHLECV
VTLKKNT

Sequences:

>Translated_407_residues
MTATIPKGFHPEPFSYHQELELDIDALSNAGDGIGRVDGWVVFVPFALPGDRVKARVWRNDRNYSSADLVEVINPSPDRV
EPGCRLFGTCGGCQYQHFSYDRQLLWKTRQVADLLRLQAGLELPVNPAVASPRQYHYRSKITPHFDRPKEGGKPAIGFLK
AGSRRDVVDVPQCPIAMECINEALPLARKSVYQAAARFKRGATILLRASEGTVITNNNAVACERVGDLEFHFLAGDFFQN
NPFILPLFTDYVAQQASMDGEEFLVDAYCGSGLFALSLAKKFKKVLGVEVSETSADWARSNARSNGIEHAEFLAADAGAI
FARVDFPAEKTAVVIDPPRKGCSAEFLAQLFAFGPGKVVYVSCNPATQIRDLAEFDKAGYFVTAVQPFDLFPQTKHLECV
VTLKKNT
>Mature_406_residues
TATIPKGFHPEPFSYHQELELDIDALSNAGDGIGRVDGWVVFVPFALPGDRVKARVWRNDRNYSSADLVEVINPSPDRVE
PGCRLFGTCGGCQYQHFSYDRQLLWKTRQVADLLRLQAGLELPVNPAVASPRQYHYRSKITPHFDRPKEGGKPAIGFLKA
GSRRDVVDVPQCPIAMECINEALPLARKSVYQAAARFKRGATILLRASEGTVITNNNAVACERVGDLEFHFLAGDFFQNN
PFILPLFTDYVAQQASMDGEEFLVDAYCGSGLFALSLAKKFKKVLGVEVSETSADWARSNARSNGIEHAEFLAADAGAIF
ARVDFPAEKTAVVIDPPRKGCSAEFLAQLFAFGPGKVVYVSCNPATQIRDLAEFDKAGYFVTAVQPFDLFPQTKHLECVV
TLKKNT

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=189, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI51173878, Length=189, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789148, Length=430, Percent_Identity=28.3720930232558, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787083, Length=194, Percent_Identity=28.3505154639175, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790403, Length=153, Percent_Identity=30.718954248366, Blast_Score=78, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322909, Length=422, Percent_Identity=34.3601895734597, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24666579, Length=160, Percent_Identity=30.625, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 44872; Mature: 44741

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATIPKGFHPEPFSYHQELELDIDALSNAGDGIGRVDGWVVFVPFALPGDRVKARVWRN
CCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
DRNYSSADLVEVINPSPDRVEPGCRLFGTCGGCQYQHFSYDRQLLWKTRQVADLLRLQAG
CCCCCCCCHHEECCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
LELPVNPAVASPRQYHYRSKITPHFDRPKEGGKPAIGFLKAGSRRDVVDVPQCPIAMECI
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHH
NEALPLARKSVYQAAARFKRGATILLRASEGTVITNNNAVACERVGDLEFHFLAGDFFQN
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCEEEEEECCCEEEEEEECHHCCC
NPFILPLFTDYVAQQASMDGEEFLVDAYCGSGLFALSLAKKFKKVLGVEVSETSADWARS
CCEEEEEHHHHHHHHHCCCCHHEEEEECCCCCHHHHHHHHHHHHHHCCEECCCCHHHHHH
NARSNGIEHAEFLAADAGAIFARVDFPAEKTAVVIDPPRKGCSAEFLAQLFAFGPGKVVY
CHHCCCCCHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEE
VSCNPATQIRDLAEFDKAGYFVTAVQPFDLFPQTKHLECVVTLKKNT
EECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
TATIPKGFHPEPFSYHQELELDIDALSNAGDGIGRVDGWVVFVPFALPGDRVKARVWRN
CCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
DRNYSSADLVEVINPSPDRVEPGCRLFGTCGGCQYQHFSYDRQLLWKTRQVADLLRLQAG
CCCCCCCCHHEECCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
LELPVNPAVASPRQYHYRSKITPHFDRPKEGGKPAIGFLKAGSRRDVVDVPQCPIAMECI
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHH
NEALPLARKSVYQAAARFKRGATILLRASEGTVITNNNAVACERVGDLEFHFLAGDFFQN
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCEEEEEECCCEEEEEEECHHCCC
NPFILPLFTDYVAQQASMDGEEFLVDAYCGSGLFALSLAKKFKKVLGVEVSETSADWARS
CCEEEEEHHHHHHHHHCCCCHHEEEEECCCCCHHHHHHHHHHHHHHCCEECCCCHHHHHH
NARSNGIEHAEFLAADAGAIFARVDFPAEKTAVVIDPPRKGCSAEFLAQLFAFGPGKVVY
CHHCCCCCHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEE
VSCNPATQIRDLAEFDKAGYFVTAVQPFDLFPQTKHLECVVTLKKNT
EECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]