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The map label for this gene is miaB

Identifier: 187735351

GI number: 187735351

Start: 1012423

End: 1013799

Strand: Reverse

Name: miaB

Synonym: Amuc_0849

Alternate gene names: 187735351

Gene position: 1013799-1012423 (Counterclockwise)

Preceding gene: 187735352

Following gene: 187735347

Centisome position: 38.05

GC content: 58.97

Gene sequence:

>1377_bases
ATGCCCAAGCTTTATATCAAAACCTACGGCTGCCAGATGAACGAACGGGACTCCGAACAGGTGGCCCGCATGTTCGTGCA
GAAGGGCTACACCATGACGGACCGCGAGGATGAGGCGGACGTCATCCTGTTCAACTCCTGTTCCATCCGGGAACAGGCGG
AACAAAAGGCTCTGGGAAAAATGGGGCTTCTGGCCAAGCAGCAGCGGCATCGTCCGCATGTAGTGTACGGCATGATGGGC
TGCATGGCCCAAAGTAAAAAAGAGGAACTGTTCAAGGAACTGCCGCGCCTGGACCTCGTGGTCGGCACCCAGAAATACCA
CCGCGTATTTGAACATGTGGACGGCATTCTGCGCGCGCGCCAGGAACGCCGCATGGATGAGTTGCAAACCGCCTTTTCCG
GTACGCATGTGTGCGATGTGGCGGAAGAGGCGGATTCCCAGAACCGAATCCGGGATCACCTGAATCCCGGCGTGCGCTCC
ACGGCGTATGTCTCCATCATGCAGGGGTGCGAAATGAAGTGCGCCTACTGCATTGTTCCCTACACCCGCGGCGCAGAACG
CAGCCGCCCCATCCGGGACGTGGTGGATGAGGTGAAGATGCTGGCGGACGCCGGCGTGAAGGAAGTCACGCTGCTGGGCC
AGATCGTCAACCGATACGGCAGGCAGATGGAAACGGCAGGCGGCAAGGGCGGCTTCGTCCAGCTGCTGGAAGCCGTGCAT
GAAGTGGAAGGAATCCGGCGCATCCGCTTCGTTTCCCCGCATCCCATCGGTTTCCGCCAGGATCTGGTGCAGGCGTTCAC
CTACCTTCCCAAGTTGTGCAGCCACATTCATTTCCCGATGCAGAGCGGCAGCGACCGCATCCTGAAAATGATGCGCAGGC
CGTACAGGAATGAAACCTATCTGGACCTTTGCTCCCGGATGAAGCAGGCACGCCCGGATTTGTCCATCACCACGGATATC
ATTGTGGGCTTCCCAGGAGAAACGGAGGAGGATTACCTGCTGACCAGGCAGGCCGTGGAACAGGTCCAGTTTGACAACGC
ATTCATTTTCCGCTATTCCCCGCGCCGCGGCACACCCGCCGCCGTGATGGAGAATCAGATTCCGGAAGAAGTGAAGGAAG
CGCGCAACCAGGATCTTCTGGCCGTAGTCAATGAAATAGCTATCCGGAAAAACCGGGATCTGGTAGGCACCGTGCAGGAA
GTTCTTCTGGAAGGGCCGTCCAAAACGAATGCGGCGCGCCTCTCCGGCCGGACCTCCCAGAACAAGCCCGTCATGGTGGA
TGCCGCCCCTGACCTGGCGGGAGAGATCCTTCCCATCCGCATTGAGGAAAGCACGGGATTTACTCTGTACGGCGTTCCAT
GCCCCTCCAGGGGATGA

Upstream 100 bases:

>100_bases
CCGGGTATTTCGTCACCGCCGTCCAGCCCTTTGACCTGTTCCCCCAGACCAAGCATCTGGAATGCGTGGTCACCCTGAAA
AAAAATACCTGATTTTTTCC

Downstream 100 bases:

>100_bases
TTTGCACGCTCTTTTCTTCCATTCGCAGATAGAGGCTTCCGGTTTCCCGGAGACGGTATACGGCTGTTCCCTCCGGTACG
GGGCATTCATGCTCCGTCGT

Product: RNA modification enzyme, MiaB family

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MPKLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILFNSCSIREQAEQKALGKMGLLAKQQRHRPHVVYGMMG
CMAQSKKEELFKELPRLDLVVGTQKYHRVFEHVDGILRARQERRMDELQTAFSGTHVCDVAEEADSQNRIRDHLNPGVRS
TAYVSIMQGCEMKCAYCIVPYTRGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNRYGRQMETAGGKGGFVQLLEAVH
EVEGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHIHFPMQSGSDRILKMMRRPYRNETYLDLCSRMKQARPDLSITTDI
IVGFPGETEEDYLLTRQAVEQVQFDNAFIFRYSPRRGTPAAVMENQIPEEVKEARNQDLLAVVNEIAIRKNRDLVGTVQE
VLLEGPSKTNAARLSGRTSQNKPVMVDAAPDLAGEILPIRIEESTGFTLYGVPCPSRG

Sequences:

>Translated_458_residues
MPKLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILFNSCSIREQAEQKALGKMGLLAKQQRHRPHVVYGMMG
CMAQSKKEELFKELPRLDLVVGTQKYHRVFEHVDGILRARQERRMDELQTAFSGTHVCDVAEEADSQNRIRDHLNPGVRS
TAYVSIMQGCEMKCAYCIVPYTRGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNRYGRQMETAGGKGGFVQLLEAVH
EVEGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHIHFPMQSGSDRILKMMRRPYRNETYLDLCSRMKQARPDLSITTDI
IVGFPGETEEDYLLTRQAVEQVQFDNAFIFRYSPRRGTPAAVMENQIPEEVKEARNQDLLAVVNEIAIRKNRDLVGTVQE
VLLEGPSKTNAARLSGRTSQNKPVMVDAAPDLAGEILPIRIEESTGFTLYGVPCPSRG
>Mature_457_residues
PKLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILFNSCSIREQAEQKALGKMGLLAKQQRHRPHVVYGMMGC
MAQSKKEELFKELPRLDLVVGTQKYHRVFEHVDGILRARQERRMDELQTAFSGTHVCDVAEEADSQNRIRDHLNPGVRST
AYVSIMQGCEMKCAYCIVPYTRGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNRYGRQMETAGGKGGFVQLLEAVHE
VEGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHIHFPMQSGSDRILKMMRRPYRNETYLDLCSRMKQARPDLSITTDII
VGFPGETEEDYLLTRQAVEQVQFDNAFIFRYSPRRGTPAAVMENQIPEEVKEARNQDLLAVVNEIAIRKNRDLVGTVQEV
LLEGPSKTNAARLSGRTSQNKPVMVDAAPDLAGEILPIRIEESTGFTLYGVPCPSRG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=487, Percent_Identity=36.3449691991786, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI28872782, Length=487, Percent_Identity=36.3449691991786, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI93277076, Length=458, Percent_Identity=28.6026200873362, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=39.4678492239468, Blast_Score=330, Evalue=9e-92,
Organism=Escherichia coli, GI1787057, Length=467, Percent_Identity=29.3361884368308, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17553146, Length=436, Percent_Identity=35.0917431192661, Blast_Score=254, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=396, Percent_Identity=26.010101010101, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356207, Length=488, Percent_Identity=34.4262295081967, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=28.4444444444444, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_AKKM8 (B2UQE7)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877463.1
- GeneID:   6274320
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0849
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   CLSK2335569
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51965; Mature: 51834

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILFNSCSIREQAEQKALGK
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
MGLLAKQQRHRPHVVYGMMGCMAQSKKEELFKELPRLDLVVGTQKYHRVFEHVDGILRAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
QERRMDELQTAFSGTHVCDVAEEADSQNRIRDHLNPGVRSTAYVSIMQGCEMKCAYCIVP
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEE
YTRGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNRYGRQMETAGGKGGFVQLLEAVH
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EVEGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHIHFPMQSGSDRILKMMRRPYRNETY
HHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHH
LDLCSRMKQARPDLSITTDIIVGFPGETEEDYLLTRQAVEQVQFDNAFIFRYSPRRGTPA
HHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
AVMENQIPEEVKEARNQDLLAVVNEIAIRKNRDLVGTVQEVLLEGPSKTNAARLSGRTSQ
HHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCC
NKPVMVDAAPDLAGEILPIRIEESTGFTLYGVPCPSRG
CCCEEEECCCCCCCCEEEEEEECCCCEEEEECCCCCCC
>Mature Secondary Structure 
PKLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILFNSCSIREQAEQKALGK
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
MGLLAKQQRHRPHVVYGMMGCMAQSKKEELFKELPRLDLVVGTQKYHRVFEHVDGILRAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
QERRMDELQTAFSGTHVCDVAEEADSQNRIRDHLNPGVRSTAYVSIMQGCEMKCAYCIVP
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEE
YTRGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNRYGRQMETAGGKGGFVQLLEAVH
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EVEGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHIHFPMQSGSDRILKMMRRPYRNETY
HHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHH
LDLCSRMKQARPDLSITTDIIVGFPGETEEDYLLTRQAVEQVQFDNAFIFRYSPRRGTPA
HHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
AVMENQIPEEVKEARNQDLLAVVNEIAIRKNRDLVGTVQEVLLEGPSKTNAARLSGRTSQ
HHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCC
NKPVMVDAAPDLAGEILPIRIEESTGFTLYGVPCPSRG
CCCEEEECCCCCCCCEEEEEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA