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The map label for this gene is ogt [H]

Identifier: 187735231

GI number: 187735231

Start: 856366

End: 856914

Strand: Direct

Name: ogt [H]

Synonym: Amuc_0725

Alternate gene names: 187735231

Gene position: 856366-856914 (Clockwise)

Preceding gene: 187735229

Following gene: 187735233

Centisome position: 32.14

GC content: 55.56

Gene sequence:

>549_bases
ATGAAAACCACTTTCCCTCCCCATGGCCGGGCGCTCCTCCACCAGATGCCTGCAGGTGCTGTCGTGATTGTGGAAAACGG
CACAGCCGTCACCCATCTTTTCTTCAGCCGCATGGCACAGCCGGAGCATTTGGAATGGGAGGAAACACCTCTGCTGCGTC
AAACGGCTGACCAGCTGGATGAATATTTTCAAGGTTCCCGCCGCGTATTTGACGTGCCGCTTTCTCCCCAAGGAACCGAA
TTTGAACGAACGGTCTGGAAAGCCCTTCAAGCCATCCCCTATGGAGAAACCAGAAGTTACGGAGACATTGCCCGGCGAAT
AGGACGCCCATCAGCATGCCGGGCAGTAGGACAAGCCAACAACCGGAACCCTGTCGGCATCATCATCCCCTGCCACCGAG
TCATCGGAGCCGACGGCAAACTGACCGGCTATGCAAGCGGCCTGACCATTAAACAATACCTGTTGGAATTGGAACAGGGC
TCCGCCACTCCTTTCACATTATTCACGGATGTGGAACGCATGCCCGGAAATACTCCGGCCAGTCCATAA

Upstream 100 bases:

>100_bases
TACATCATTCGGGACCACATACCTCCTTTCCCCTGTCAAATGGCGAAATATCCTGCAAAATGAATAGAACCCTTAACCCG
GCGCATGCTATGGTCATGAT

Downstream 100 bases:

>100_bases
AACGGCAGGACTGGAAAATTCCGGTCAGAGCTGCATACCGTTTTCCGTCTGAGGAAGAGGCATGGATTTCAAGGCGGCCT
TGGGGGATTCAATACCCAGC

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKTTFPPHGRALLHQMPAGAVVIVENGTAVTHLFFSRMAQPEHLEWEETPLLRQTADQLDEYFQGSRRVFDVPLSPQGTE
FERTVWKALQAIPYGETRSYGDIARRIGRPSACRAVGQANNRNPVGIIIPCHRVIGADGKLTGYASGLTIKQYLLELEQG
SATPFTLFTDVERMPGNTPASP

Sequences:

>Translated_182_residues
MKTTFPPHGRALLHQMPAGAVVIVENGTAVTHLFFSRMAQPEHLEWEETPLLRQTADQLDEYFQGSRRVFDVPLSPQGTE
FERTVWKALQAIPYGETRSYGDIARRIGRPSACRAVGQANNRNPVGIIIPCHRVIGADGKLTGYASGLTIKQYLLELEQG
SATPFTLFTDVERMPGNTPASP
>Mature_182_residues
MKTTFPPHGRALLHQMPAGAVVIVENGTAVTHLFFSRMAQPEHLEWEETPLLRQTADQLDEYFQGSRRVFDVPLSPQGTE
FERTVWKALQAIPYGETRSYGDIARRIGRPSACRAVGQANNRNPVGIIIPCHRVIGADGKLTGYASGLTIKQYLLELEQG
SATPFTLFTDVERMPGNTPASP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=116, Percent_Identity=39.6551724137931, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=50, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788542, Length=87, Percent_Identity=50.5747126436782, Blast_Score=91, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=90, Percent_Identity=52.2222222222222, Blast_Score=93, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=90, Percent_Identity=52.2222222222222, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=41.5730337078652, Blast_Score=92, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137554, Length=120, Percent_Identity=36.6666666666667, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20118; Mature: 20118

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTFPPHGRALLHQMPAGAVVIVENGTAVTHLFFSRMAQPEHLEWEETPLLRQTADQLD
CCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EYFQGSRRVFDVPLSPQGTEFERTVWKALQAIPYGETRSYGDIARRIGRPSACRAVGQAN
HHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCC
NRNPVGIIIPCHRVIGADGKLTGYASGLTIKQYLLELEQGSATPFTLFTDVERMPGNTPA
CCCCEEEEEECHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCC
SP
CC
>Mature Secondary Structure
MKTTFPPHGRALLHQMPAGAVVIVENGTAVTHLFFSRMAQPEHLEWEETPLLRQTADQLD
CCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EYFQGSRRVFDVPLSPQGTEFERTVWKALQAIPYGETRSYGDIARRIGRPSACRAVGQAN
HHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCC
NRNPVGIIIPCHRVIGADGKLTGYASGLTIKQYLLELEQGSATPFTLFTDVERMPGNTPA
CCCCEEEEEECHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCC
SP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]