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The map label for this gene is cysL [H]

Identifier: 187735207

GI number: 187735207

Start: 825435

End: 826358

Strand: Direct

Name: cysL [H]

Synonym: Amuc_0701

Alternate gene names: 187735207

Gene position: 825435-826358 (Clockwise)

Preceding gene: 187735205

Following gene: 187735208

Centisome position: 30.98

GC content: 54.44

Gene sequence:

>924_bases
ATGGTTGAATATTATTTCCTCTCCATGATTACGGATTTCCGGCTGAAAGTGTTTGAAACCGTCGCACGGCGCCTGAATTT
TACGCGCGCGGCGGAAGAACTGTTCATCACGCAGCCGGCCGTCACCCGGCATATCAAGGAACTGGAACGTCTGCTGGATG
CCCGTCTGTTCAAAAGGGAGGGACGAGGGATTGCCCTGACGGCGGAAGGCGTGCGCCTGCTGGCCCATTCCCGCCGCATC
CTCAAGGAATACGAAACGCTGGCTGAAGAAATATCCTTTGGCCGCAATTCCCCTGTTTCCGGCGAATTGAGCCTGGGAGC
CAGCTCCACCATGTCCCAGTATGTGCTGCCTCCGCTGCTGGCCCTGTTCAACCGCCATTACCCGGAAATACGCATATGGC
TGAAAAGCGGCAATACGGAGGAAATAGAACAGGCCGTCCAGGATGAATCCGTGCAGCTTGGCATCGTGGAAGGCACGGCA
ACGCGCGCGAACCTGCATTACCAGCCATTTATGGAGGACGAAATTATTCTGGCAGGAGGCATGCAGGCCGCCGTGCCCAG
TGAAGGGCTTTCCCTCCCGCAACTCTCCCATGTGCCCCTGATCATGCGTGAACCGGGGTCCGGCACGCGTCGGGTGGTGG
AGGAAGCGCTGAAAAAACACGGGATCTCCGTTTCCGGCCTCCATGTTCTGATGATGCTGGGCAATACGGAAAGCATCAAA
ATGTATCTGGAGCATTCTCCGGCGTGCTCTTTTCTCTCCTCCCTTGCTGTCCGGGGAGAATTCAAACGCGGAAGTTTGAA
GAAAATACATCTTCGCCACCTGGAAATTCGCCGCACCTTCCACTTCGTCTCAGGACATGGAGACCACAGCAGAATCAATG
AACTGTTCATCAAGTTCTGCCGATATTATTATAACAAAATTTAA

Upstream 100 bases:

>100_bases
CCGGAATATTACGGGCACCCGCGCGTCTTGCACTTGAGCGCCATTCCCAGCGCCGTACGCCATCATGCGGAGGAAAGGAG
CTTGCCGTCCCCCCGGCGGC

Downstream 100 bases:

>100_bases
TTGGCTATAACAATTTTTAATTAGATTTTATAATGGCTGCATCGTATATTATTCCGCAGCCAATTCGGTACGCTCCCGGC
CAGATACAACGGGCAGCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MVEYYFLSMITDFRLKVFETVARRLNFTRAAEELFITQPAVTRHIKELERLLDARLFKREGRGIALTAEGVRLLAHSRRI
LKEYETLAEEISFGRNSPVSGELSLGASSTMSQYVLPPLLALFNRHYPEIRIWLKSGNTEEIEQAVQDESVQLGIVEGTA
TRANLHYQPFMEDEIILAGGMQAAVPSEGLSLPQLSHVPLIMREPGSGTRRVVEEALKKHGISVSGLHVLMMLGNTESIK
MYLEHSPACSFLSSLAVRGEFKRGSLKKIHLRHLEIRRTFHFVSGHGDHSRINELFIKFCRYYYNKI

Sequences:

>Translated_307_residues
MVEYYFLSMITDFRLKVFETVARRLNFTRAAEELFITQPAVTRHIKELERLLDARLFKREGRGIALTAEGVRLLAHSRRI
LKEYETLAEEISFGRNSPVSGELSLGASSTMSQYVLPPLLALFNRHYPEIRIWLKSGNTEEIEQAVQDESVQLGIVEGTA
TRANLHYQPFMEDEIILAGGMQAAVPSEGLSLPQLSHVPLIMREPGSGTRRVVEEALKKHGISVSGLHVLMMLGNTESIK
MYLEHSPACSFLSSLAVRGEFKRGSLKKIHLRHLEIRRTFHFVSGHGDHSRINELFIKFCRYYYNKI
>Mature_307_residues
MVEYYFLSMITDFRLKVFETVARRLNFTRAAEELFITQPAVTRHIKELERLLDARLFKREGRGIALTAEGVRLLAHSRRI
LKEYETLAEEISFGRNSPVSGELSLGASSTMSQYVLPPLLALFNRHYPEIRIWLKSGNTEEIEQAVQDESVQLGIVEGTA
TRANLHYQPFMEDEIILAGGMQAAVPSEGLSLPQLSHVPLIMREPGSGTRRVVEEALKKHGISVSGLHVLMMLGNTESIK
MYLEHSPACSFLSSLAVRGEFKRGSLKKIHLRHLEIRRTFHFVSGHGDHSRINELFIKFCRYYYNKI

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=30.1369863013699, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082132, Length=254, Percent_Identity=28.3464566929134, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI145693105, Length=205, Percent_Identity=30.2439024390244, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=27.2357723577236, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=206, Percent_Identity=30.0970873786408, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788296, Length=234, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788706, Length=163, Percent_Identity=33.7423312883436, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789173, Length=144, Percent_Identity=34.7222222222222, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=216, Percent_Identity=26.3888888888889, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788297, Length=204, Percent_Identity=26.4705882352941, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789506, Length=215, Percent_Identity=25.1162790697674, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=160, Percent_Identity=33.75, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=125, Percent_Identity=28.8, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=304, Percent_Identity=23.3552631578947, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787530, Length=244, Percent_Identity=20.9016393442623, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789639, Length=124, Percent_Identity=33.8709677419355, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=128, Percent_Identity=26.5625, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788887, Length=163, Percent_Identity=26.9938650306748, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788626, Length=214, Percent_Identity=27.5700934579439, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367136, Length=124, Percent_Identity=35.4838709677419, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI48994962, Length=106, Percent_Identity=34.9056603773585, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34956; Mature: 34956

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEYYFLSMITDFRLKVFETVARRLNFTRAAEELFITQPAVTRHIKELERLLDARLFKRE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GRGIALTAEGVRLLAHSRRILKEYETLAEEISFGRNSPVSGELSLGASSTMSQYVLPPLL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHH
ALFNRHYPEIRIWLKSGNTEEIEQAVQDESVQLGIVEGTATRANLHYQPFMEDEIILAGG
HHHCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCEECCCEECCCCCCCEEEECC
MQAAVPSEGLSLPQLSHVPLIMREPGSGTRRVVEEALKKHGISVSGLHVLMMLGNTESIK
CCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEE
MYLEHSPACSFLSSLAVRGEFKRGSLKKIHLRHLEIRRTFHFVSGHGDHSRINELFIKFC
EEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RYYYNKI
HHHHHCC
>Mature Secondary Structure
MVEYYFLSMITDFRLKVFETVARRLNFTRAAEELFITQPAVTRHIKELERLLDARLFKRE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GRGIALTAEGVRLLAHSRRILKEYETLAEEISFGRNSPVSGELSLGASSTMSQYVLPPLL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHH
ALFNRHYPEIRIWLKSGNTEEIEQAVQDESVQLGIVEGTATRANLHYQPFMEDEIILAGG
HHHCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCEECCCEECCCCCCCEEEECC
MQAAVPSEGLSLPQLSHVPLIMREPGSGTRRVVEEALKKHGISVSGLHVLMMLGNTESIK
CCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEE
MYLEHSPACSFLSSLAVRGEFKRGSLKKIHLRHLEIRRTFHFVSGHGDHSRINELFIKFC
EEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RYYYNKI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]