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The map label for this gene is mpl [H]

Identifier: 187735187

GI number: 187735187

Start: 799714

End: 801096

Strand: Direct

Name: mpl [H]

Synonym: Amuc_0681

Alternate gene names: 187735187

Gene position: 799714-801096 (Clockwise)

Preceding gene: 187735186

Following gene: 187735188

Centisome position: 30.02

GC content: 57.92

Gene sequence:

>1383_bases
ATGAACAGCCATTTCCACTTTCTCGGCATCTGCGGCACCGCCATGGGCGCAGTAGCCGCCGCCATGGCCCGCCGCGGCTT
TACCGTCACCGGTTCCGACGCCAACGTCTATCCCCCCATGTCCACTTTTCTGGAGGAGGAAGGCATCACGCTGTTTGAAG
GCTACAAGGCCTCCAACATCCCGCCGGATGCGGACGTCATCGTCATCGGCAATGCCATGAGCCGCGGCAATGAGGAAGTG
GAAGCCGTGCTCCAACGGCGCCTGCGTTACCTTTCCCTCCCGGAGACGATGAAGGAATACTTCCTGCGCGGCAAGCGCAA
CTATGTGGTAACAGGCACCCACGGAAAAACCACCACCACATCCATGCTGGCGTGGCTGATGGAGGATTCCGGGCTGAACC
CCAGCTTCATGATTGGCGGCATTGCCCGCAATCTCGGACGCGGGGGCCGTTTTACGGATTCCGATTTTTCCGTACTGGAG
GGGGACGAGTACGACACGGCGTTTTTCGACAAACGTTCCAAATTTCTGCACTACCTCCCGGAACTGGTGGTCATCAACAA
CATTGAATTTGATCACGCGGATATTTACAATTCCCTGGAGGAAATCAAATTGAGTTTCCGACGGCTGACCCAGATCATCC
CCCGCAACGGCCTGGCCCTGGTCAACGCGGACGATCCCAACTGCCTGGACGTGGTTAAGGGGGCCCCGTGCCCCGTCAAG
ACGGTGGGCTTTTCCGACTCTTCCAACATCCGTATCCTGGATGTGGAGACCTCCACGGACGGCAGCCATTTCACACTGGA
AGGAACCCGGTACTACGTACCGATGATTGGAGAGTTCAACATCCGCAACGCCGCCATGGCAATTGCAGCCGCACAATTTG
CGGGACTGAACGTCAAGCAGCTGGCGGATTCCATGAGCCGCTTTGAAGGCGTGGCCCGCCGCCAGGAAGTCAAGGGGGTG
GTCAACGGCATTTCCGTAGTGGACGACTTCGCCCACCACCCAACAGCCATCAAACAGGCCATCCTGGGCCTACGCCAGCG
CTATCCGGAATCACGCATCTGGGCCATTTTCGAACCGCGCTCCAACACCACACGCAGAAATATCTTCCAGAATGAACTGG
CGTTGTCCCTCGCTACGGCGGATTTCCCTGTGGTAGCCGCCGTGGATCATCCGGACAAGGTAGCTCTGGAGGAACGCCTG
GACCTCACCAAACTTTCCAACGACCTTTCCGCGCTGGGCAAGGACGCTCTGATTGGCGGCCATGCCCAGGAAATCGTCTC
TGCCGTCTGCAAGAAAGCCCAGCCGGGAGATGTTATTCTGGTAATGAGCAACGGCGGTTTTGAAGGCATTCACGCCAAAT
TGCTGGAAGCACTGAACGCCTGA

Upstream 100 bases:

>100_bases
GCCAACGGCATTGTGCGCCGCCGGGACCGGGCTCTGGCGGATTCCTGTATTGCGGCTCCTTCCTTTTCCAAATAAAACCC
GCTCCATTCCTCTCCCCGTC

Downstream 100 bases:

>100_bases
CCCTTCTTCATACATCCACTTCCAATGAAAAACGCATCCACCATCACCGCCTTCATCATCAGCTTCCTGGTCGTAGGCAG
CCTGGCCCTGGTTTTCCTGT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNSHFHFLGICGTAMGAVAAAMARRGFTVTGSDANVYPPMSTFLEEEGITLFEGYKASNIPPDADVIVIGNAMSRGNEEV
EAVLQRRLRYLSLPETMKEYFLRGKRNYVVTGTHGKTTTTSMLAWLMEDSGLNPSFMIGGIARNLGRGGRFTDSDFSVLE
GDEYDTAFFDKRSKFLHYLPELVVINNIEFDHADIYNSLEEIKLSFRRLTQIIPRNGLALVNADDPNCLDVVKGAPCPVK
TVGFSDSSNIRILDVETSTDGSHFTLEGTRYYVPMIGEFNIRNAAMAIAAAQFAGLNVKQLADSMSRFEGVARRQEVKGV
VNGISVVDDFAHHPTAIKQAILGLRQRYPESRIWAIFEPRSNTTRRNIFQNELALSLATADFPVVAAVDHPDKVALEERL
DLTKLSNDLSALGKDALIGGHAQEIVSAVCKKAQPGDVILVMSNGGFEGIHAKLLEALNA

Sequences:

>Translated_460_residues
MNSHFHFLGICGTAMGAVAAAMARRGFTVTGSDANVYPPMSTFLEEEGITLFEGYKASNIPPDADVIVIGNAMSRGNEEV
EAVLQRRLRYLSLPETMKEYFLRGKRNYVVTGTHGKTTTTSMLAWLMEDSGLNPSFMIGGIARNLGRGGRFTDSDFSVLE
GDEYDTAFFDKRSKFLHYLPELVVINNIEFDHADIYNSLEEIKLSFRRLTQIIPRNGLALVNADDPNCLDVVKGAPCPVK
TVGFSDSSNIRILDVETSTDGSHFTLEGTRYYVPMIGEFNIRNAAMAIAAAQFAGLNVKQLADSMSRFEGVARRQEVKGV
VNGISVVDDFAHHPTAIKQAILGLRQRYPESRIWAIFEPRSNTTRRNIFQNELALSLATADFPVVAAVDHPDKVALEERL
DLTKLSNDLSALGKDALIGGHAQEIVSAVCKKAQPGDVILVMSNGGFEGIHAKLLEALNA
>Mature_460_residues
MNSHFHFLGICGTAMGAVAAAMARRGFTVTGSDANVYPPMSTFLEEEGITLFEGYKASNIPPDADVIVIGNAMSRGNEEV
EAVLQRRLRYLSLPETMKEYFLRGKRNYVVTGTHGKTTTTSMLAWLMEDSGLNPSFMIGGIARNLGRGGRFTDSDFSVLE
GDEYDTAFFDKRSKFLHYLPELVVINNIEFDHADIYNSLEEIKLSFRRLTQIIPRNGLALVNADDPNCLDVVKGAPCPVK
TVGFSDSSNIRILDVETSTDGSHFTLEGTRYYVPMIGEFNIRNAAMAIAAAQFAGLNVKQLADSMSRFEGVARRQEVKGV
VNGISVVDDFAHHPTAIKQAILGLRQRYPESRIWAIFEPRSNTTRRNIFQNELALSLATADFPVVAAVDHPDKVALEERL
DLTKLSNDLSALGKDALIGGHAQEIVSAVCKKAQPGDVILVMSNGGFEGIHAKLLEALNA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=459, Percent_Identity=42.483660130719, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786279, Length=470, Percent_Identity=29.3617021276596, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50413; Mature: 50413

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHFHFLGICGTAMGAVAAAMARRGFTVTGSDANVYPPMSTFLEEEGITLFEGYKASNI
CCCCEEEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCEEEECCCCCCC
PPDADVIVIGNAMSRGNEEVEAVLQRRLRYLSLPETMKEYFLRGKRNYVVTGTHGKTTTT
CCCCCEEEEECHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHHH
SMLAWLMEDSGLNPSFMIGGIARNLGRGGRFTDSDFSVLEGDEYDTAFFDKRSKFLHYLP
HHHHHHHHCCCCCCHHEEHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCC
ELVVINNIEFDHADIYNSLEEIKLSFRRLTQIIPRNGLALVNADDPNCLDVVKGAPCPVK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCEE
TVGFSDSSNIRILDVETSTDGSHFTLEGTRYYVPMIGEFNIRNAAMAIAAAQFAGLNVKQ
EECCCCCCCEEEEEEEECCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
LADSMSRFEGVARRQEVKGVVNGISVVDDFAHHPTAIKQAILGLRQRYPESRIWAIFEPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCC
SNTTRRNIFQNELALSLATADFPVVAAVDHPDKVALEERLDLTKLSNDLSALGKDALIGG
CCCHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHEECC
HAQEIVSAVCKKAQPGDVILVMSNGGFEGIHAKLLEALNA
CHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNSHFHFLGICGTAMGAVAAAMARRGFTVTGSDANVYPPMSTFLEEEGITLFEGYKASNI
CCCCEEEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCEEEECCCCCCC
PPDADVIVIGNAMSRGNEEVEAVLQRRLRYLSLPETMKEYFLRGKRNYVVTGTHGKTTTT
CCCCCEEEEECHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHHH
SMLAWLMEDSGLNPSFMIGGIARNLGRGGRFTDSDFSVLEGDEYDTAFFDKRSKFLHYLP
HHHHHHHHCCCCCCHHEEHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCC
ELVVINNIEFDHADIYNSLEEIKLSFRRLTQIIPRNGLALVNADDPNCLDVVKGAPCPVK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCEE
TVGFSDSSNIRILDVETSTDGSHFTLEGTRYYVPMIGEFNIRNAAMAIAAAQFAGLNVKQ
EECCCCCCCEEEEEEEECCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
LADSMSRFEGVARRQEVKGVVNGISVVDDFAHHPTAIKQAILGLRQRYPESRIWAIFEPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCC
SNTTRRNIFQNELALSLATADFPVVAAVDHPDKVALEERLDLTKLSNDLSALGKDALIGG
CCCHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHEECC
HAQEIVSAVCKKAQPGDVILVMSNGGFEGIHAKLLEALNA
CHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]