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The map label for this gene is leuC

Identifier: 187735172

GI number: 187735172

Start: 783434

End: 784846

Strand: Direct

Name: leuC

Synonym: Amuc_0666

Alternate gene names: 187735172

Gene position: 783434-784846 (Clockwise)

Preceding gene: 187735171

Following gene: 187735173

Centisome position: 29.41

GC content: 61.22

Gene sequence:

>1413_bases
ATGGGGAAAACGCTTTTCCAAAAAATCTGGGACGCTCATACCGTCGGCATCCTGCCGGATGGGAGAACGCAAATGTTCAT
CGCTACGCACCTGCTGCATGAAGTCACCTCTCCGCAGGCTTTCGGAATGGTCCGGGACCTGGGCCTGACTGTGCGCCACC
CGGAACGCACCTTTGCCACTGTGGACCACATCATTCCCACAGACAACCAGGCGGAACCGTTCGCGGACGCCACGGCTGAC
GCCATGATCAGGGAACTGCGCCGGAACTGCGCTGAAAACGGCATCCGCTTTTTCGACCTCCCTACCGGGCTCCAGGGCAT
CGTGCATATGGTAGGGCCGGAACTCGGCATCACTCAGCCGGGCATGACTATCGTATGCGGAGACTCCCATACGGCCACCC
ACGGAGCCTTCGGTGCCATTGCCATGGGCATCGGCACCACGCAGGTGCGCGATGTGCTGGCTACGCAGACCCTGGCCCTC
AGCCCGCTCAAGGTGCGCCGCATCAATGTGAACGGAAAGCTGGCCCCCGGCGTGCGCGCCAAGGATGTAGCCCTGCACAT
CATCGGCCTTCTGGGAGCCAAGGGCGGCCTGGGCTTCGCCTACGAATACGGAGGCGAGGTCATTGACGCCATGAGCATGG
ACGAACGCATGACCCTCTGCAACATGTCCATTGAAGGCGCGGCGCGCTGCGGTTACGTGAACCCTGACCGGACCACGGTG
GAATACATCAAAGGACGCCTGTTCGCCCCCACCGGCGCGGACTGGGACAAGGCCGTGGAACGCTGGCTGGGCTTTGCTTC
CGACGCAGATGCGGAATATGATGAAATCGTGGAAATTGACGGAGCTTCCATTGAGCCTACATTGACATGGGGCATTTCTC
CGGACCAGAATACGGGCATCAGCGGCAGCACTCCCAACCCATCCGACGCAGCGGACGACGATGAACGGAAGATGATCAAT
GAAGCGCTGGAATACATGAAATTCCCCGCGGACATGCCTCTTAAGGGGCTGCCGGTTCAAGTGTGCTTCGTAGGTTCCTG
CACCAATGGGCGCATTTCAGACTTCCGGGAAGTGGCCGCCCTCATCAAGGGTCGCCATGTGGCCCCCGGCATCAGGGCGC
TGGCCGTTCCCGGCTCCCAGATGACTGCCCGGCAGTGTGAAGAGGAAGGCATCGCGGACATTTTCCGTGAAGCCGGCTTT
GAATGGCGTCTGGCGGGTTGCTCCATGTGCCTGGCCATGAATCCGGACAAGCTCCAGGGTGACCAGCTCTGCGCCAGTTC
CTCCAACCGGAACTTCAAGGGCCGGCAGGGAAGCCCCACCGGACGCACCCTGCTGATGAGCCCGGCCATGGTGGCCGCCG
CTGCTCTGACCGGGAAAGTCTCCGATGCCCGCGAAGTGTTCTCCCTGAATTAA

Upstream 100 bases:

>100_bases
AAATGTTCAAAAAAACTCCTTATGTAAGACACAGACCGTGTCAGCAATACGTCCTTGCATCTTGACGCGGTTCCCGAACG
GACGGATGCTTATGTCAGTT

Downstream 100 bases:

>100_bases
CTTTCCAATTTTTTCCTTTATGTCTCTGACCCAATTCACCTCCATCACCGGCACCTGCGTTCCCGTTCCGGGACCGGACA
TGGACACGGACCGCATCATC

Product: 3-isopropylmalate dehydratase, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MGKTLFQKIWDAHTVGILPDGRTQMFIATHLLHEVTSPQAFGMVRDLGLTVRHPERTFATVDHIIPTDNQAEPFADATAD
AMIRELRRNCAENGIRFFDLPTGLQGIVHMVGPELGITQPGMTIVCGDSHTATHGAFGAIAMGIGTTQVRDVLATQTLAL
SPLKVRRINVNGKLAPGVRAKDVALHIIGLLGAKGGLGFAYEYGGEVIDAMSMDERMTLCNMSIEGAARCGYVNPDRTTV
EYIKGRLFAPTGADWDKAVERWLGFASDADAEYDEIVEIDGASIEPTLTWGISPDQNTGISGSTPNPSDAADDDERKMIN
EALEYMKFPADMPLKGLPVQVCFVGSCTNGRISDFREVAALIKGRHVAPGIRALAVPGSQMTARQCEEEGIADIFREAGF
EWRLAGCSMCLAMNPDKLQGDQLCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALTGKVSDAREVFSLN

Sequences:

>Translated_470_residues
MGKTLFQKIWDAHTVGILPDGRTQMFIATHLLHEVTSPQAFGMVRDLGLTVRHPERTFATVDHIIPTDNQAEPFADATAD
AMIRELRRNCAENGIRFFDLPTGLQGIVHMVGPELGITQPGMTIVCGDSHTATHGAFGAIAMGIGTTQVRDVLATQTLAL
SPLKVRRINVNGKLAPGVRAKDVALHIIGLLGAKGGLGFAYEYGGEVIDAMSMDERMTLCNMSIEGAARCGYVNPDRTTV
EYIKGRLFAPTGADWDKAVERWLGFASDADAEYDEIVEIDGASIEPTLTWGISPDQNTGISGSTPNPSDAADDDERKMIN
EALEYMKFPADMPLKGLPVQVCFVGSCTNGRISDFREVAALIKGRHVAPGIRALAVPGSQMTARQCEEEGIADIFREAGF
EWRLAGCSMCLAMNPDKLQGDQLCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALTGKVSDAREVFSLN
>Mature_469_residues
GKTLFQKIWDAHTVGILPDGRTQMFIATHLLHEVTSPQAFGMVRDLGLTVRHPERTFATVDHIIPTDNQAEPFADATADA
MIRELRRNCAENGIRFFDLPTGLQGIVHMVGPELGITQPGMTIVCGDSHTATHGAFGAIAMGIGTTQVRDVLATQTLALS
PLKVRRINVNGKLAPGVRAKDVALHIIGLLGAKGGLGFAYEYGGEVIDAMSMDERMTLCNMSIEGAARCGYVNPDRTTVE
YIKGRLFAPTGADWDKAVERWLGFASDADAEYDEIVEIDGASIEPTLTWGISPDQNTGISGSTPNPSDAADDDERKMINE
ALEYMKFPADMPLKGLPVQVCFVGSCTNGRISDFREVAALIKGRHVAPGIRALAVPGSQMTARQCEEEGIADIFREAGFE
WRLAGCSMCLAMNPDKLQGDQLCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALTGKVSDAREVFSLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=366, Percent_Identity=27.3224043715847, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI8659555, Length=411, Percent_Identity=24.0875912408759, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI41352693, Length=377, Percent_Identity=24.4031830238727, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=51.2875536480687, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787531, Length=403, Percent_Identity=25.0620347394541, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=26.9972451790634, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=26.9972451790634, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=26.4150943396226, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17568399, Length=461, Percent_Identity=22.9934924078091, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=48.5106382978723, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=29.700272479564, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=409, Percent_Identity=28.8508557457213, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=489, Percent_Identity=26.1758691206544, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.4869109947644, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365315, Length=383, Percent_Identity=27.4151436031332, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17864292, Length=383, Percent_Identity=27.4151436031332, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571643, Length=384, Percent_Identity=25.2604166666667, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_AKKM8 (B2UPW8)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877284.1
- GeneID:   6273972
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0666
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50531; Mature: 50400

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLFQKIWDAHTVGILPDGRTQMFIATHLLHEVTSPQAFGMVRDLGLTVRHPERTFAT
CCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHH
VDHIIPTDNQAEPFADATADAMIRELRRNCAENGIRFFDLPTGLQGIVHMVGPELGITQP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCC
GMTIVCGDSHTATHGAFGAIAMGIGTTQVRDVLATQTLALSPLKVRRINVNGKLAPGVRA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCH
KDVALHIIGLLGAKGGLGFAYEYGGEVIDAMSMDERMTLCNMSIEGAARCGYVNPDRTTV
HHHHHHHHHHHCCCCCCCEEEHHCCHHHHHHCCCCHHHEECCCCCCCCCCCCCCCCCHHH
EYIKGRLFAPTGADWDKAVERWLGFASDADAEYDEIVEIDGASIEPTLTWGISPDQNTGI
HHHCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCEEEECCCCCCCCCC
SGSTPNPSDAADDDERKMINEALEYMKFPADMPLKGLPVQVCFVGSCTNGRISDFREVAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
LIKGRHVAPGIRALAVPGSQMTARQCEEEGIADIFREAGFEWRLAGCSMCLAMNPDKLQG
HHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCCC
DQLCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALTGKVSDAREVFSLN
CHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
GKTLFQKIWDAHTVGILPDGRTQMFIATHLLHEVTSPQAFGMVRDLGLTVRHPERTFAT
CHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHH
VDHIIPTDNQAEPFADATADAMIRELRRNCAENGIRFFDLPTGLQGIVHMVGPELGITQP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCC
GMTIVCGDSHTATHGAFGAIAMGIGTTQVRDVLATQTLALSPLKVRRINVNGKLAPGVRA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCH
KDVALHIIGLLGAKGGLGFAYEYGGEVIDAMSMDERMTLCNMSIEGAARCGYVNPDRTTV
HHHHHHHHHHHCCCCCCCEEEHHCCHHHHHHCCCCHHHEECCCCCCCCCCCCCCCCCHHH
EYIKGRLFAPTGADWDKAVERWLGFASDADAEYDEIVEIDGASIEPTLTWGISPDQNTGI
HHHCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCEEEECCCCCCCCCC
SGSTPNPSDAADDDERKMINEALEYMKFPADMPLKGLPVQVCFVGSCTNGRISDFREVAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
LIKGRHVAPGIRALAVPGSQMTARQCEEEGIADIFREAGFEWRLAGCSMCLAMNPDKLQG
HHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCCC
DQLCASSSNRNFKGRQGSPTGRTLLMSPAMVAAAALTGKVSDAREVFSLN
CHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA