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The map label for this gene is lipB [H]

Identifier: 187735171

GI number: 187735171

Start: 782593

End: 783231

Strand: Direct

Name: lipB [H]

Synonym: Amuc_0665

Alternate gene names: 187735171

Gene position: 782593-783231 (Clockwise)

Preceding gene: 187735170

Following gene: 187735172

Centisome position: 29.38

GC content: 57.43

Gene sequence:

>639_bases
ATGAACATCATCCGCATTCCCGGCCTGGCAGATTATCAGGAAACCTTGAGGCTCCAGGAACAACTGGTCCGGGCCCATCA
GGAAGACCCGGACAGGGAAAACGAACTGTTGCTGCTGGAACATGCCGGAGTTTATACCATCGGGCGTACACGGAACCACG
CCAGCCTGCACCCCGGTTCCGTACTTCCCTTTCCCGTTCATGAAATCAACCGCGGCGGCCAGGCTACCTACCACGGCCCC
GGCCAGCTGGTCGGATACCCCATTACGGACCTGAACCGCTGCGGCAGGGATCTGCACCGTTACGTCACCACCCTGGAGAA
CACCCTGATTGAAACCTGTGCCCGGTTCGGCGTGCAGGCCCGCATCCGGGAAGGGCTGGTAGGGGTCTGGGTGGAAAACC
GCAAAATAGCCTCCATCGGCGTCGGGGTGAAAAAGTGGATTGCCATGCACGGCTTCGCCCTCAATATAACCCGGAAATCC
CTGCCTCCCTTCCTTGCTATTACCCCATGCGGCATCCAGGGCGTGCAAATGACCTGCCTGGAGGATGAAATGTCCTCCCC
CCTGCCGGAAGGAAACCTGTTGAAATGTTTCGGAGACGTTTTTGCAGCACTATGGGAAGAAAAATTTAACCGGTCTTAA

Upstream 100 bases:

>100_bases
CCCTGGCCGCCGCCGTGCACCAGTACCGCACCGCCCTGGGCCAGCAAGTGCGCAGAACGCGCTGAGTTCCCTAACTGTCC
CGGGGCCTCCCTTCAGCAGC

Downstream 100 bases:

>100_bases
GGGGATTCGGAACGTGCGGAGGGCATGAAAACCATCCTTTCCTTAAAGGCGGAAAGGCCCCAAAGGCTGGAGAGAGGCCC
GTTCAGACCATGAATGCAGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNIIRIPGLADYQETLRLQEQLVRAHQEDPDRENELLLLEHAGVYTIGRTRNHASLHPGSVLPFPVHEINRGGQATYHGP
GQLVGYPITDLNRCGRDLHRYVTTLENTLIETCARFGVQARIREGLVGVWVENRKIASIGVGVKKWIAMHGFALNITRKS
LPPFLAITPCGIQGVQMTCLEDEMSSPLPEGNLLKCFGDVFAALWEEKFNRS

Sequences:

>Translated_212_residues
MNIIRIPGLADYQETLRLQEQLVRAHQEDPDRENELLLLEHAGVYTIGRTRNHASLHPGSVLPFPVHEINRGGQATYHGP
GQLVGYPITDLNRCGRDLHRYVTTLENTLIETCARFGVQARIREGLVGVWVENRKIASIGVGVKKWIAMHGFALNITRKS
LPPFLAITPCGIQGVQMTCLEDEMSSPLPEGNLLKCFGDVFAALWEEKFNRS
>Mature_212_residues
MNIIRIPGLADYQETLRLQEQLVRAHQEDPDRENELLLLEHAGVYTIGRTRNHASLHPGSVLPFPVHEINRGGQATYHGP
GQLVGYPITDLNRCGRDLHRYVTTLENTLIETCARFGVQARIREGLVGVWVENRKIASIGVGVKKWIAMHGFALNITRKS
LPPFLAITPCGIQGVQMTCLEDEMSSPLPEGNLLKCFGDVFAALWEEKFNRS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=199, Percent_Identity=38.1909547738693, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI87081767, Length=177, Percent_Identity=36.1581920903955, Blast_Score=107, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323268, Length=129, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355483, Length=210, Percent_Identity=36.1904761904762, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23736; Mature: 23736

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIRIPGLADYQETLRLQEQLVRAHQEDPDRENELLLLEHAGVYTIGRTRNHASLHPGS
CCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCC
VLPFPVHEINRGGQATYHGPGQLVGYPITDLNRCGRDLHRYVTTLENTLIETCARFGVQA
CCCCCHHHHCCCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RIREGLVGVWVENRKIASIGVGVKKWIAMHGFALNITRKSLPPFLAITPCGIQGVQMTCL
HHHCCEEEEEECCCEEEECCCCHHHHHHHCCEEEEEEHHCCCCEEEEECCCCCCEEEEEE
EDEMSSPLPEGNLLKCFGDVFAALWEEKFNRS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIIRIPGLADYQETLRLQEQLVRAHQEDPDRENELLLLEHAGVYTIGRTRNHASLHPGS
CCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCC
VLPFPVHEINRGGQATYHGPGQLVGYPITDLNRCGRDLHRYVTTLENTLIETCARFGVQA
CCCCCHHHHCCCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RIREGLVGVWVENRKIASIGVGVKKWIAMHGFALNITRKSLPPFLAITPCGIQGVQMTCL
HHHCCEEEEEECCCEEEECCCCHHHHHHHCCEEEEEEHHCCCCEEEEECCCCCCEEEEEE
EDEMSSPLPEGNLLKCFGDVFAALWEEKFNRS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA