BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is fabG [H]

Identifier: 186476926

GI number: 186476926

Start: 2437704

End: 2438462

Strand: Direct

Name: fabG [H]

Synonym: Bphy_2175

Alternate gene names: 186476926

Gene position: 2437704-2438462 (Clockwise)

Preceding gene: 186476925

Following gene: 186476927

Centisome position: 70.07

GC content: 65.09

Gene sequence:

>759_bases
ATGCGGCTGACGGGTAAAACGGCTATCGTCACGGGCGGCGGCTCGGGCTTCGGCGAAGGCATCGCGAAGACTTATGCGCG
CGAAGGCGCGAACGTCGTCGTCAACGATCTGAACGGCGCGGCGGCCGAACGCGTCGCGAGCGAGATCGCGCTGGGGGGCG
GGAAGGCCATCGCGGTCGCGGGCGACGTGACGCGTGCGGAGGACTGGCATGCGCTGCGCCTCGCCGCGATCGAAGACTTC
GGCAACGTGCAAATCGTGGTCAACAACGCGGGCACGACGCATCGCAACAAGCCGGTGCTCGATGTGACAGAGGCTGAGTT
CGACCGGGTGTATGCCGTCAACGTGAAGAGCATTTACTGGAGCGTGCGCGAATTCGTGCCGTATTTCCGCGAGCAAGGCG
GCGGCGTATTCGTCAACATCGCGTCGACGGCGGGGGTGCGGCCGCGGCCGGGCCTCGTCTGGTACAACGGCAGCAAGGGC
GCGGTGATCGTCGCGAGCAAGGCACTCGCCGTCGAACTCGGGCCGGACCGCATCCGTGTGAACTGCGTCAACCCTGTGAT
CGGCGAAACGGGGCTGTTGACCGAGTTCATGGGCGTCGAAGACACGCCCGAGAACCGGCAGAAGTTTCTGGCAGGCATTC
CGTTGGGCCGCTTCTCGACACCGCAAGACATCGCGAACGCCGCGCTATATCTCGCTTCGGACGAAGCAGAGTTCATCACG
GGCGTGTGTCTCGAAGTGGACGGCGGACGCTGCGTGTAG

Upstream 100 bases:

>100_bases
GGTGTTGAAGACCGTGGCGATCCGGCACGGCTGACGCACAGCGGGTTCGAACGGCTCCGGCGCCTGCCGGAGTGCACAAC
GCTAACGACAAGGAGACATC

Downstream 100 bases:

>100_bases
CTCCGTGCAAGCGGCAGGGCAAGCGGCGCGCCTCGGCACTTCGTGCGCGCCGCCTGTCAAAGCGCGTTAATCGTTGAAAT
CTCGGTGTTTTCACTGACGC

Product: 3-ketoacyl-(acyl-carrier-protein) reductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRLTGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALGGGKAIAVAGDVTRAEDWHALRLAAIEDF
GNVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNVKSIYWSVREFVPYFREQGGGVFVNIASTAGVRPRPGLVWYNGSKG
AVIVASKALAVELGPDRIRVNCVNPVIGETGLLTEFMGVEDTPENRQKFLAGIPLGRFSTPQDIANAALYLASDEAEFIT
GVCLEVDGGRCV

Sequences:

>Translated_252_residues
MRLTGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALGGGKAIAVAGDVTRAEDWHALRLAAIEDF
GNVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNVKSIYWSVREFVPYFREQGGGVFVNIASTAGVRPRPGLVWYNGSKG
AVIVASKALAVELGPDRIRVNCVNPVIGETGLLTEFMGVEDTPENRQKFLAGIPLGRFSTPQDIANAALYLASDEAEFIT
GVCLEVDGGRCV
>Mature_252_residues
MRLTGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALGGGKAIAVAGDVTRAEDWHALRLAAIEDF
GNVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNVKSIYWSVREFVPYFREQGGGVFVNIASTAGVRPRPGLVWYNGSKG
AVIVASKALAVELGPDRIRVNCVNPVIGETGLLTEFMGVEDTPENRQKFLAGIPLGRFSTPQDIANAALYLASDEAEFIT
GVCLEVDGGRCV

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=33.2, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI66933014, Length=254, Percent_Identity=33.8582677165354, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=29.6442687747036, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=32.5301204819277, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI33667109, Length=228, Percent_Identity=32.8947368421053, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=35.1351351351351, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI59889578, Length=229, Percent_Identity=32.7510917030568, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=28.735632183908, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=32.046332046332, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI10190704, Length=249, Percent_Identity=30.9236947791165, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=30.7377049180328, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI142976729, Length=194, Percent_Identity=27.8350515463918, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4504505, Length=246, Percent_Identity=26.8292682926829, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI210032110, Length=216, Percent_Identity=28.7037037037037, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4758504, Length=259, Percent_Identity=30.8880308880309, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI302344755, Length=109, Percent_Identity=36.697247706422, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI40807363, Length=195, Percent_Identity=28.7179487179487, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI310128830, Length=202, Percent_Identity=29.2079207920792, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310115468, Length=202, Percent_Identity=29.2079207920792, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI40254986, Length=162, Percent_Identity=29.0123456790123, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=27.8225806451613, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI83715985, Length=261, Percent_Identity=29.8850574712644, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25282469, Length=217, Percent_Identity=25.3456221198157, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI31542939, Length=246, Percent_Identity=25.2032520325203, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI210032112, Length=216, Percent_Identity=26.3888888888889, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=40.9638554216867, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=33.596837944664, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=29.9212598425197, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=30.6451612903226, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=33.0677290836653, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=31.8548387096774, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=32.9457364341085, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=31.1284046692607, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=28.8617886178862, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=28.6852589641434, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787526, Length=251, Percent_Identity=26.2948207171315, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787820, Length=231, Percent_Identity=26.4069264069264, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=30.5220883534137, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI115534694, Length=253, Percent_Identity=33.596837944664, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=33.7349397590361, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=33.0645161290323, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=31.0483870967742, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=34, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=33.2, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=30.8823529411765, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17568967, Length=204, Percent_Identity=36.7647058823529, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17564282, Length=192, Percent_Identity=31.25, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531453, Length=272, Percent_Identity=28.6764705882353, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562904, Length=272, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=30.5660377358491, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=29.9242424242424, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=29.9242424242424, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560332, Length=269, Percent_Identity=30.4832713754647, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=28.030303030303, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17544670, Length=273, Percent_Identity=27.4725274725275, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=28.735632183908, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=28.030303030303, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=28.996282527881, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=27.5471698113208, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508895, Length=194, Percent_Identity=29.3814432989691, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=27.1698113207547, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=267, Percent_Identity=28.8389513108614, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=27.9245283018868, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=26.0377358490566, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193203117, Length=195, Percent_Identity=28.2051282051282, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=257, Percent_Identity=30.7392996108949, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=191, Percent_Identity=28.7958115183246, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=194, Percent_Identity=26.8041237113402, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=245, Percent_Identity=24.0816326530612, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71982365, Length=200, Percent_Identity=25, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=34.3589743589744, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324126, Length=252, Percent_Identity=25.7936507936508, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323882, Length=217, Percent_Identity=27.1889400921659, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=34.9206349206349, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=34.375, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=35.1778656126482, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=33.4677419354839, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24639444, Length=251, Percent_Identity=33.0677290836653, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643142, Length=255, Percent_Identity=32.5490196078431, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=34.2629482071713, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=32.9896907216495, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24644337, Length=262, Percent_Identity=32.0610687022901, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281364892, Length=197, Percent_Identity=32.994923857868, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24649181, Length=188, Percent_Identity=31.9148936170213, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859837, Length=216, Percent_Identity=30.5555555555556, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17737361, Length=255, Percent_Identity=31.3725490196078, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357673, Length=195, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355631, Length=227, Percent_Identity=27.7533039647577, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581726, Length=196, Percent_Identity=28.0612244897959, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161076933, Length=192, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584748, Length=192, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574402, Length=186, Percent_Identity=30.6451612903226, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584744, Length=186, Percent_Identity=30.6451612903226, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221510726, Length=198, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665243, Length=201, Percent_Identity=29.3532338308458, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221331218, Length=201, Percent_Identity=29.3532338308458, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 26611; Mature: 26611

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALGGGKAIAVA
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
GDVTRAEDWHALRLAAIEDFGNVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNVKSIYW
CCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEHHHHHH
SVREFVPYFREQGGGVFVNIASTAGVRPRPGLVWYNGSKGAVIVASKALAVELGPDRIRV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCEEEE
NCVNPVIGETGLLTEFMGVEDTPENRQKFLAGIPLGRFSTPQDIANAALYLASDEAEFIT
EECCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHCEEEEEECCCCCEEE
GVCLEVDGGRCV
EEEEEECCCCCC
>Mature Secondary Structure
MRLTGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALGGGKAIAVA
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
GDVTRAEDWHALRLAAIEDFGNVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNVKSIYW
CCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEHHHHHH
SVREFVPYFREQGGGVFVNIASTAGVRPRPGLVWYNGSKGAVIVASKALAVELGPDRIRV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCEEEE
NCVNPVIGETGLLTEFMGVEDTPENRQKFLAGIPLGRFSTPQDIANAALYLASDEAEFIT
EECCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHCEEEEEECCCCCEEE
GVCLEVDGGRCV
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]