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The map label for this gene is purD [H]

Identifier: 186476819

GI number: 186476819

Start: 2316767

End: 2318044

Strand: Direct

Name: purD [H]

Synonym: Bphy_2065

Alternate gene names: 186476819

Gene position: 2316767-2318044 (Clockwise)

Preceding gene: 186476818

Following gene: 186476820

Centisome position: 66.59

GC content: 64.48

Gene sequence:

>1278_bases
ATGAAGTTACTCGTCGTCGGTTCCGGCGGTCGCGAGCATGCGCTCGCGTGGAAGCTCGCGCAATCGCAGCGCGTGCAACT
CGTCTATGTCGCGCCAGGCAACGGCGGCACCGCCCAGGACGACCGTCTGCGCAACATCGACATCACTGATCCGAACGCAC
TCGCCGACTTCGTCGAGAAGGAACAGATCGCCTTCACGCTGGTGGGTCCGGAACAGCCGCTCGCAGCGGGTATCGTCAAC
ATCTTCCGCGCTCGCGGCCTGAAGATCTTCGGCCCGACGAGGGAAGCGGCGCAGCTCGAAAGCTCGAAGGACTTCGCGAA
GGCGTTCATGAAGCGCCACGCAATTCCGACGGCCGAGTACGAGACCTTTTCCGACGTGGCGGCCGCGCATGCGTACATCG
ACGCAAAGGGCGCGCCCATCGTCATCAAGGCCGATGGCCTTGCGGCGGGCAAGGGCGTGGTTGTCGCGCTGACGCTCGAC
GAGGCGCACCACGCCGTCGACATGATGCTGTCGGACAACAAACTCGGCGACGCCGGCGCACGTGTCGTGATTGAAGAGTT
TCTGACGGGCGAAGAAGCGAGCTTCATCGTGATGGTGGACGGCAAGAACGTGCTGGCGCTGGCGTCGAGCCAGGATCACA
AGCGCCTGCTGGACGGCGATCAGGGACCGAACACGGGCGGCATGGGCGCCTACTCGCCCGCGCCCATCGTCACGCCGCAA
CTGCACGCACGCGTGATGCGCGAAATCATCCTGCCGACCGTGCGCGGCATGGAGAAAGAAGGCATTCGCTTCACGGGCTT
CCTGTACGCCGGTTTGATGATCGACGCGCAAGGCAACCCGAAGACGCTCGAATTCAACTGCCGCATGGGCGACCCGGAAA
CGCAGCCGATCATGGCGCGTCTGAAGGGTGACTTCTCGAAGGTCGTCGAGCAGGCGATCGCGGGTACGCTGGACACAGTC
GAACTCGAATGGGACCGCCGCACCGCGCTTGGCGTGGTGCTGGCCGCGCACGACTATCCGGAAGCGCCGCGCAAGGGCGA
TTTCATCAGCGGCATTCCGGCGGAGACGGCGGACGCGGTGACGTTCCACGCAGGCACGACGCTCAAGGACGGCAAGCTGA
CGACCTCGGGCGGACGTGTGCTGTGCGTCGTCGGACTGGCGGATTCCGTGCGCAGCGCGCAGTCGGTCGCTTATGAGGCG
ATCAACCACATTTCGTTCGACGGCATGCAGTATCGCCGCGACATCGGTTATCGCGCGGCCAACCGCAAGCACGGCTAA

Upstream 100 bases:

>100_bases
TCACATGTCACGCGTTCGCGCTGTGCGAAGGTAAAGATGGACATCCACGGCTCGCGCCAGTGCGCGGGCCGTTCACGGTT
TTTAGTATTCGGGGAATCAC

Downstream 100 bases:

>100_bases
GCCGGCCGTCTTCGCATATTGGCTGTCTGGCGCGGCCGTTCGGGGCACGCTGCCCGGACCCGCGAAACAACCCTTCGGGG
CCATATGGCCGCGCCGGCGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKLLVVGSGGREHALAWKLAQSQRVQLVYVAPGNGGTAQDDRLRNIDITDPNALADFVEKEQIAFTLVGPEQPLAAGIVN
IFRARGLKIFGPTREAAQLESSKDFAKAFMKRHAIPTAEYETFSDVAAAHAYIDAKGAPIVIKADGLAAGKGVVVALTLD
EAHHAVDMMLSDNKLGDAGARVVIEEFLTGEEASFIVMVDGKNVLALASSQDHKRLLDGDQGPNTGGMGAYSPAPIVTPQ
LHARVMREIILPTVRGMEKEGIRFTGFLYAGLMIDAQGNPKTLEFNCRMGDPETQPIMARLKGDFSKVVEQAIAGTLDTV
ELEWDRRTALGVVLAAHDYPEAPRKGDFISGIPAETADAVTFHAGTTLKDGKLTTSGGRVLCVVGLADSVRSAQSVAYEA
INHISFDGMQYRRDIGYRAANRKHG

Sequences:

>Translated_425_residues
MKLLVVGSGGREHALAWKLAQSQRVQLVYVAPGNGGTAQDDRLRNIDITDPNALADFVEKEQIAFTLVGPEQPLAAGIVN
IFRARGLKIFGPTREAAQLESSKDFAKAFMKRHAIPTAEYETFSDVAAAHAYIDAKGAPIVIKADGLAAGKGVVVALTLD
EAHHAVDMMLSDNKLGDAGARVVIEEFLTGEEASFIVMVDGKNVLALASSQDHKRLLDGDQGPNTGGMGAYSPAPIVTPQ
LHARVMREIILPTVRGMEKEGIRFTGFLYAGLMIDAQGNPKTLEFNCRMGDPETQPIMARLKGDFSKVVEQAIAGTLDTV
ELEWDRRTALGVVLAAHDYPEAPRKGDFISGIPAETADAVTFHAGTTLKDGKLTTSGGRVLCVVGLADSVRSAQSVAYEA
INHISFDGMQYRRDIGYRAANRKHG
>Mature_425_residues
MKLLVVGSGGREHALAWKLAQSQRVQLVYVAPGNGGTAQDDRLRNIDITDPNALADFVEKEQIAFTLVGPEQPLAAGIVN
IFRARGLKIFGPTREAAQLESSKDFAKAFMKRHAIPTAEYETFSDVAAAHAYIDAKGAPIVIKADGLAAGKGVVVALTLD
EAHHAVDMMLSDNKLGDAGARVVIEEFLTGEEASFIVMVDGKNVLALASSQDHKRLLDGDQGPNTGGMGAYSPAPIVTPQ
LHARVMREIILPTVRGMEKEGIRFTGFLYAGLMIDAQGNPKTLEFNCRMGDPETQPIMARLKGDFSKVVEQAIAGTLDTV
ELEWDRRTALGVVLAAHDYPEAPRKGDFISGIPAETADAVTFHAGTTLKDGKLTTSGGRVLCVVGLADSVRSAQSVAYEA
INHISFDGMQYRRDIGYRAANRKHG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=50.1182033096927, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=50.1182033096927, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=50.1182033096927, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=50.1182033096927, Blast_Score=403, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=60.5140186915888, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=43.2624113475177, Blast_Score=333, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=43.5374149659864, Blast_Score=334, Evalue=1e-92,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=46.6200466200466, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=46.6200466200466, Blast_Score=351, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45592; Mature: 45592

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHALAWKLAQSQRVQLVYVAPGNGGTAQDDRLRNIDITDPNALADFVEK
CEEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
EQIAFTLVGPEQPLAAGIVNIFRARGLKIFGPTREAAQLESSKDFAKAFMKRHAIPTAEY
CEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCC
ETFSDVAAAHAYIDAKGAPIVIKADGLAAGKGVVVALTLDEAHHAVDMMLSDNKLGDAGA
HHHHHHHHHHHEEECCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHH
RVVIEEFLTGEEASFIVMVDGKNVLALASSQDHKRLLDGDQGPNTGGMGAYSPAPIVTPQ
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARVMREIILPTVRGMEKEGIRFTGFLYAGLMIDAQGNPKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
LKGDFSKVVEQAIAGTLDTVELEWDRRTALGVVLAAHDYPEAPRKGDFISGIPAETADAV
HHCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLKDGKLTTSGGRVLCVVGLADSVRSAQSVAYEAINHISFDGMQYRRDIGYRAA
EEECCCEECCCEEEECCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
NRKHG
CCCCC
>Mature Secondary Structure
MKLLVVGSGGREHALAWKLAQSQRVQLVYVAPGNGGTAQDDRLRNIDITDPNALADFVEK
CEEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
EQIAFTLVGPEQPLAAGIVNIFRARGLKIFGPTREAAQLESSKDFAKAFMKRHAIPTAEY
CEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCC
ETFSDVAAAHAYIDAKGAPIVIKADGLAAGKGVVVALTLDEAHHAVDMMLSDNKLGDAGA
HHHHHHHHHHHEEECCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHH
RVVIEEFLTGEEASFIVMVDGKNVLALASSQDHKRLLDGDQGPNTGGMGAYSPAPIVTPQ
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARVMREIILPTVRGMEKEGIRFTGFLYAGLMIDAQGNPKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
LKGDFSKVVEQAIAGTLDTVELEWDRRTALGVVLAAHDYPEAPRKGDFISGIPAETADAV
HHCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLKDGKLTTSGGRVLCVVGLADSVRSAQSVAYEAINHISFDGMQYRRDIGYRAA
EEECCCEECCCEEEECCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
NRKHG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]