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The map label for this gene is potF [H]

Identifier: 186476038

GI number: 186476038

Start: 1435143

End: 1436243

Strand: Reverse

Name: potF [H]

Synonym: Bphy_1279

Alternate gene names: 186476038

Gene position: 1436243-1435143 (Counterclockwise)

Preceding gene: 186476041

Following gene: 186476037

Centisome position: 41.28

GC content: 60.13

Gene sequence:

>1101_bases
ATGAAGAAACGGGTGGTGGGCCAACTGGCTGCGCTGGTACTGTGCGCGACGCCTTTCATGACGGCGGCGGCAAAAGATAC
ACAACTGAATGTGTACAACTGGTCGGACTACATCGCCAAGGACACGATCCCGAACTTCACAAAGCAGACGGGCGTCCAGG
TCAAGTACGACAACTACGATAGCGACGACACGCTGCAGGCCAAGCTGCTGACGGGCAATTCGGGCTACGACATCGTCGTG
CCGACCAGCAACTACGCAGGCAAGCAGATCGCAGCGGGCATCTTCGCGCCGCTCGACAAGTCGAAGATCCCCAATCTCAA
GTACCTCGATCCGGCGCTAATGGCGCTCGTCGCGGGTGCCGACCCGGGCAACAGGTTCACGGTGCCCTGGGCGTACGGCA
CGACGGGCCTCGGCTACAACGTGACGAAGGCGCAGCAGATTCTCGGCAAGGGCGTCGCGCTCGACAACTGGGACATCCTG
TTCAAGCCGGAAAACATCTCGAAGCTCAAGGCGTGCGGCGTCTCGGTACTCGACGCGCCGGACCAGATGTTCGCGGCCGC
GCTGCATTACATCGGTAAGGATCCGATGAGCACGAATCCCGCTGATTATCGTGAAGCCCTCGCGATGATGAAGAAGATCC
GTCCGTACATCACGCAGTTCAACTCGTCGGGCTATATCAACGACATGGTTGGCGGCGACGTGTGTTTTGCATATGGCTGG
TCGGGCGACGTCGTGATCGCGAAGCATCGCGCGGCGGAAGCGAAGAAGCCATACAAGATCGACTATTACATTCCGAAGGG
CGGCGCGCCCGTGTGGTTCGACGTGATGGCGATTCCGAAGGACGCGAAGAACAAGGAAGCGGCCACGGAGTGGATCAACT
ACATCGAGACGCCGCAGGTTCATGCGGCCATCACCAACGCGGTGTACTACCCGAGCGCAAATGCGGAAGCGCGCAAGTAC
GTGGACAAGGATGTCGCGAATGATCCCGCCGTCTATCCGCCGGCGGACGTCGTCAAGACGCTGTTCCTGCTCAAGCCGCT
GCCGGCTGAGATCCAGCGTCTGCAGACGCGGCTGTGGACCGAGTTCAAATCCGGCCGCTAA

Upstream 100 bases:

>100_bases
ATGGTAGTTTTCGGGTTTTTCAAGGGTAGCCGCCGCGGGTGCGCAGCGGCTTGAAGCGGGGTTAGCGACCGGTTTCGCGC
CGGTCAGAACGGAGAACGGA

Downstream 100 bases:

>100_bases
GCCCGTCTGAAGTAGCCGGTCGCGCAGGAAAGCATCATCATGAAGCCCTCGGTATCCAGCCGGGGGCTTTGTTCGTCTAC
ATGCAGGGAGAGAAGCAGCA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKKRVVGQLAALVLCATPFMTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVKYDNYDSDDTLQAKLLTGNSGYDIVV
PTSNYAGKQIAAGIFAPLDKSKIPNLKYLDPALMALVAGADPGNRFTVPWAYGTTGLGYNVTKAQQILGKGVALDNWDIL
FKPENISKLKACGVSVLDAPDQMFAAALHYIGKDPMSTNPADYREALAMMKKIRPYITQFNSSGYINDMVGGDVCFAYGW
SGDVVIAKHRAAEAKKPYKIDYYIPKGGAPVWFDVMAIPKDAKNKEAATEWINYIETPQVHAAITNAVYYPSANAEARKY
VDKDVANDPAVYPPADVVKTLFLLKPLPAEIQRLQTRLWTEFKSGR

Sequences:

>Translated_366_residues
MKKRVVGQLAALVLCATPFMTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVKYDNYDSDDTLQAKLLTGNSGYDIVV
PTSNYAGKQIAAGIFAPLDKSKIPNLKYLDPALMALVAGADPGNRFTVPWAYGTTGLGYNVTKAQQILGKGVALDNWDIL
FKPENISKLKACGVSVLDAPDQMFAAALHYIGKDPMSTNPADYREALAMMKKIRPYITQFNSSGYINDMVGGDVCFAYGW
SGDVVIAKHRAAEAKKPYKIDYYIPKGGAPVWFDVMAIPKDAKNKEAATEWINYIETPQVHAAITNAVYYPSANAEARKY
VDKDVANDPAVYPPADVVKTLFLLKPLPAEIQRLQTRLWTEFKSGR
>Mature_366_residues
MKKRVVGQLAALVLCATPFMTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVKYDNYDSDDTLQAKLLTGNSGYDIVV
PTSNYAGKQIAAGIFAPLDKSKIPNLKYLDPALMALVAGADPGNRFTVPWAYGTTGLGYNVTKAQQILGKGVALDNWDIL
FKPENISKLKACGVSVLDAPDQMFAAALHYIGKDPMSTNPADYREALAMMKKIRPYITQFNSSGYINDMVGGDVCFAYGW
SGDVVIAKHRAAEAKKPYKIDYYIPKGGAPVWFDVMAIPKDAKNKEAATEWINYIETPQVHAAITNAVYYPSANAEARKY
VDKDVANDPAVYPPADVVKTLFLLKPLPAEIQRLQTRLWTEFKSGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=48.5013623978202, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=34.8765432098765, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787711, Length=297, Percent_Identity=22.5589225589226, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40276; Mature: 40276

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRVVGQLAALVLCATPFMTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVKYDNYD
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCC
SDDTLQAKLLTGNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKIPNLKYLDPALMALVAGA
CCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
DPGNRFTVPWAYGTTGLGYNVTKAQQILGKGVALDNWDILFKPENISKLKACGVSVLDAP
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEECCCEEEECCCCHHHHHHCCCCEECCC
DQMFAAALHYIGKDPMSTNPADYREALAMMKKIRPYITQFNSSGYINDMVGGDVCFAYGW
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECC
SGDVVIAKHRAAEAKKPYKIDYYIPKGGAPVWFDVMAIPKDAKNKEAATEWINYIETPQV
CCCEEEEECCHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCH
HAAITNAVYYPSANAEARKYVDKDVANDPAVYPPADVVKTLFLLKPLPAEIQRLQTRLWT
HHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
EFKSGR
HHCCCC
>Mature Secondary Structure
MKKRVVGQLAALVLCATPFMTAAAKDTQLNVYNWSDYIAKDTIPNFTKQTGVQVKYDNYD
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHCCCCCCHHHCCCEEEECCCC
SDDTLQAKLLTGNSGYDIVVPTSNYAGKQIAAGIFAPLDKSKIPNLKYLDPALMALVAGA
CCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCC
DPGNRFTVPWAYGTTGLGYNVTKAQQILGKGVALDNWDILFKPENISKLKACGVSVLDAP
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEECCCEEEECCCCHHHHHHCCCCEECCC
DQMFAAALHYIGKDPMSTNPADYREALAMMKKIRPYITQFNSSGYINDMVGGDVCFAYGW
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECC
SGDVVIAKHRAAEAKKPYKIDYYIPKGGAPVWFDVMAIPKDAKNKEAATEWINYIETPQV
CCCEEEEECCHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCH
HAAITNAVYYPSANAEARKYVDKDVANDPAVYPPADVVKTLFLLKPLPAEIQRLQTRLWT
HHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
EFKSGR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]