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The map label for this gene is kgtP [H]

Identifier: 186475985

GI number: 186475985

Start: 1373764

End: 1375059

Strand: Reverse

Name: kgtP [H]

Synonym: Bphy_1226

Alternate gene names: 186475985

Gene position: 1375059-1373764 (Counterclockwise)

Preceding gene: 186475986

Following gene: 186475982

Centisome position: 39.52

GC content: 62.11

Gene sequence:

>1296_bases
ATGACCGATACGACCGTCTTCACGCCCTCCGACACCCGTCGACGCATCTTCGCGATCGTCGGCGCTTCGTCCGGCAATCT
CGTCGAGTGGTTCGATTTCTACGTCTACTCGTTTTGCGCACTGTACTTCGCACCCGCGTTTTTCCCGAAAGGCGATACGA
CAGCGCAGTTGCTGAATACGGCAGGCGTGTTCGCCGCGGGCTTCCTGATGCGCCCGATCGGCGGCTGGTTCTTCGGCCGT
CTCGCCGACAAGCGCGGCCGCCGTTTCGCGATGATGGTGTCTGTATTCATGATGTGCGGCGGTTCGCTCGTGATTGCCGC
GTTGCCCACTTATGCGCAGATCGGCGCGCTCGCGCCTGCGTTGCTGCTGGTCGCGCGTCTGTTTCAGGGCCTGTCGGTGG
GCGGCGAATATGGCACCAGCGCAACGTACATGAGTGAAGTCGCGTTGAAGGGGCGTCGCGGCTTCTTCGCGTCGTTCCAG
TACGTGACGCTGATCGGCGGGCAACTGTTCGCGTTGCTCGTGCTCGTGATCCTGCAACAGCTGCTGACGACGGAAGAGCT
GAAGGCGTGGGGCTGGCGTATTCCGTTCGTGGTCGGCGCGCTGGCCGCGCTGGTCGCGCTGTATCTGCGCCGCTCGCTCG
ACGAAACGACCACGGCCGAGACGCGTCAGCGCAAGGAAGCGGGCACGCTGCGCGGCATGTGGAAGCACAAGGGCGCTTTC
TTCACGGTGCTCGGCTTTACGGCGGGCGGTTCGCTGATTTTCTACACGTTTACGACGTACATGCAGAAGTATCTCGTCAA
CACAGCAGGCATGAACACGAAGACTGCCAGCAACGTGATGACGGCTGCGCTCTTCGTGTACATGATCATGCAGCCGGTGT
TCGGTGCCTTGTCGGATCGTATCGGCCGCCGCCAGTCTATGCTGCTGTTCGGCTTCTTCGCGACGATCAGCACGGTGCCG
CTGCTGCACGCCCTGAAGGACGTGACAAGCCCCGTGATGGCCTTCGTGTTCGTGGTGATCGCGCTGGCCATTGTCAGCTT
CTATACGTCGATCAGCGGCCTGATCAAGGCCGAAATGTTCCCACCTGAAGTGCGCGCGCTGGGCGTCGGCCTGTCGTACG
CGGTGGCGAATGCGATCTTCGGCGGGTCGGCGGAGTACGTGGCGCTGTGGCTCAAGCAGGCCGGCAGCGAATCGATGTTC
TACTGGTACGTGACGGCGCTGTGTGCGATCGCCGGCCTCGTATCGTTCCGCATGCGCGATCCGTCAAAGGAAGGCTATCT
GCGCAACGAACCGTGA

Upstream 100 bases:

>100_bases
CCCGTTGAAACGCGATCGAAACCGCGTTGAAACCGGGCTGGGGCGCAACACAAAGGCGGCATTCGACCGCTGAAACTTCA
AAGAAGGGACCGACACCAGC

Downstream 100 bases:

>100_bases
GGCGTCGCGCCTGACGCGCGATCATGAAGTACCGGAAGCGGCTCTTCCATAGAGCCGCTTTTTTTCGCCTGACGCTCGCG
CGTGCGCACTCAGGGAAAGC

Product: major facilitator superfamily metabolite/H(+) symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTDTTVFTPSDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPKGDTTAQLLNTAGVFAAGFLMRPIGGWFFGR
LADKRGRRFAMMVSVFMMCGGSLVIAALPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQ
YVTLIGGQLFALLVLVILQQLLTTEELKAWGWRIPFVVGALAALVALYLRRSLDETTTAETRQRKEAGTLRGMWKHKGAF
FTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMNTKTASNVMTAALFVYMIMQPVFGALSDRIGRRQSMLLFGFFATISTVP
LLHALKDVTSPVMAFVFVVIALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSESMF
YWYVTALCAIAGLVSFRMRDPSKEGYLRNEP

Sequences:

>Translated_431_residues
MTDTTVFTPSDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPKGDTTAQLLNTAGVFAAGFLMRPIGGWFFGR
LADKRGRRFAMMVSVFMMCGGSLVIAALPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQ
YVTLIGGQLFALLVLVILQQLLTTEELKAWGWRIPFVVGALAALVALYLRRSLDETTTAETRQRKEAGTLRGMWKHKGAF
FTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMNTKTASNVMTAALFVYMIMQPVFGALSDRIGRRQSMLLFGFFATISTVP
LLHALKDVTSPVMAFVFVVIALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSESMF
YWYVTALCAIAGLVSFRMRDPSKEGYLRNEP
>Mature_430_residues
TDTTVFTPSDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPKGDTTAQLLNTAGVFAAGFLMRPIGGWFFGRL
ADKRGRRFAMMVSVFMMCGGSLVIAALPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQY
VTLIGGQLFALLVLVILQQLLTTEELKAWGWRIPFVVGALAALVALYLRRSLDETTTAETRQRKEAGTLRGMWKHKGAFF
TVLGFTAGGSLIFYTFTTYMQKYLVNTAGMNTKTASNVMTAALFVYMIMQPVFGALSDRIGRRQSMLLFGFFATISTVPL
LHALKDVTSPVMAFVFVVIALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSESMFY
WYVTALCAIAGLVSFRMRDPSKEGYLRNEP

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=70.4491725768322, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=29.5348837209302, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788292, Length=430, Percent_Identity=29.0697674418605, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789941, Length=439, Percent_Identity=32.8018223234624, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87082231, Length=324, Percent_Identity=27.1604938271605, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=368, Percent_Identity=25.5434782608696, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47234; Mature: 47103

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTVFTPSDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPKGDTTAQLLNT
CCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AGVFAAGFLMRPIGGWFFGRLADKRGRRFAMMVSVFMMCGGSLVIAALPTYAQIGALAPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
LLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQYVTLIGGQLFALLVLVILQQ
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTTEELKAWGWRIPFVVGALAALVALYLRRSLDETTTAETRQRKEAGTLRGMWKHKGAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCE
FTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMNTKTASNVMTAALFVYMIMQPVFGALSDR
EEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRRQSMLLFGFFATISTVPLLHALKDVTSPVMAFVFVVIALAIVSFYTSISGLIKAEMF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSESMFYWYVTALCAIAGLVSFRMRD
CCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
PSKEGYLRNEP
CCCCCCCCCCC
>Mature Secondary Structure 
TDTTVFTPSDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPKGDTTAQLLNT
CCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AGVFAAGFLMRPIGGWFFGRLADKRGRRFAMMVSVFMMCGGSLVIAALPTYAQIGALAPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
LLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQYVTLIGGQLFALLVLVILQQ
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTTEELKAWGWRIPFVVGALAALVALYLRRSLDETTTAETRQRKEAGTLRGMWKHKGAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCE
FTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMNTKTASNVMTAALFVYMIMQPVFGALSDR
EEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRRQSMLLFGFFATISTVPLLHALKDVTSPVMAFVFVVIALAIVSFYTSISGLIKAEMF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSESMFYWYVTALCAIAGLVSFRMRD
CCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
PSKEGYLRNEP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]