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The map label for this gene is gltP [H]

Identifier: 183222782

GI number: 183222782

Start: 3562264

End: 3563526

Strand: Direct

Name: gltP [H]

Synonym: LEPBI_I3441

Alternate gene names: 183222782

Gene position: 3562264-3563526 (Clockwise)

Preceding gene: 183222781

Following gene: 183222783

Centisome position: 98.96

GC content: 36.98

Gene sequence:

>1263_bases
ATGTCCCTTCCCAAAATTGCCTTTTGGAAACAAATTCTCATCTCTTTAGTGTTAGGTTTAGCATTAGGAATTTTATTAAA
TCCGGAAACAGGATTTATTACTTCTGACGCGATCAATCCATATTTAAGTTGGTTAAAACTTCCAGGTGATCTATTCCTAA
ATCTTCTGCAAATGATCATGATCCCACTTGTGATAGTATCCATTGCACTTGGTGTATCCAGTCTAAAAAATTTAAAGGAT
CTCTGGAGTTTAGGAAGTAAAACATTATTCTACTTTATTTTTACAACAATCGTTTCGGTGAGTATCGGGATTTCACTCAC
AACAGTTACAAAACCTGGATACCAAATCCAATCTAAAATCCAAACTCAAACCATTACAAATCCAATGAGCACACTGGAGA
AAAAGGAAAAAGAAGAATCCATTCCAGAAATCATCGCAAATATCATTCCGAAGAATATTGTGAATGTTTGGTCGAAACAA
CAGATGTTATCAATTGTATTTTTAGGAATGATTCTTGGGGTTTTTTTTCTCACTTCAAAAGAATCAGGAACAGCACTCAA
AGCGTTTTGCCATTCATTAGAAAGTTTTTGTTTATGGGTCGTTTCTACTGCGATGAAACTGGCACCACTCGCAGTTTTCG
GATTGATGAGTTATGCGATGATCCAAATTGGTTTTTCATTAGTGTTTGGTCTTTTGACCTATATATTGACAGTGTTAGGT
GGACTTTGCCTCATTCTTGTTTTTTACAGTTTCATGGTTTTCTTTTTTACCAAAAAAAATCCTTTTCTATTTTTCAATCA
AATCCGTGAAATTCCCATTTTAGGTTTTTCAACGTCTAGTTCCAGTTCTGTTTTGCCATACTCATTAAAGATAGCCAAAG
AAAAGTTAAGGTTAAAAGAATCAGTTGCCGATTTTGTTCTCCCACTCGGGGCCACAATCAATATGGACGGAACAGCCTTA
TACCAAGCCGTAGCCACAGTTTTCCTTAGCCAAGTGTACCAAATTGAATTAAGTTCAGTAGAGTTGTTTTTGTTAGCTGG
AACTGTAACGGCAGCTTCGATTGGAACCGCGGCGACCCCTGGTGTTGGCCTTGTGATTTTGTCATCTATCCTTTATACCT
TTCACATTCCTATCGAAGGGATCACCATTCTTTTTGGTGTTGACCGGTTTTTGGATATGTGTAGAACTTCAGTAAATCTG
ACCGGCGATCTCTCGTGCGCTTTTATCATGGACCATATCTGGAAGGAAGAAAAACAATTATGA

Upstream 100 bases:

>100_bases
CAAAAATGGAGCAAAAATTTTCCACCGAATCCAACTCTTCCAAACTTATATCTGCATTTAAATTCATTTACATAGAATTG
AAAATTTAAAAAATTGGGGA

Downstream 100 bases:

>100_bases
AAAATATAAGATTAATAGTTTTGATAGGTATCTCTTTATTTTTGATTCACTGTGACAAAAAAAAAGACCCTCTCAATGAC
CTAGAAACCAATCTCCTTTG

Product: putative proton glutamate symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSLPKIAFWKQILISLVLGLALGILLNPETGFITSDAINPYLSWLKLPGDLFLNLLQMIMIPLVIVSIALGVSSLKNLKD
LWSLGSKTLFYFIFTTIVSVSIGISLTTVTKPGYQIQSKIQTQTITNPMSTLEKKEKEESIPEIIANIIPKNIVNVWSKQ
QMLSIVFLGMILGVFFLTSKESGTALKAFCHSLESFCLWVVSTAMKLAPLAVFGLMSYAMIQIGFSLVFGLLTYILTVLG
GLCLILVFYSFMVFFFTKKNPFLFFNQIREIPILGFSTSSSSSVLPYSLKIAKEKLRLKESVADFVLPLGATINMDGTAL
YQAVATVFLSQVYQIELSSVELFLLAGTVTAASIGTAATPGVGLVILSSILYTFHIPIEGITILFGVDRFLDMCRTSVNL
TGDLSCAFIMDHIWKEEKQL

Sequences:

>Translated_420_residues
MSLPKIAFWKQILISLVLGLALGILLNPETGFITSDAINPYLSWLKLPGDLFLNLLQMIMIPLVIVSIALGVSSLKNLKD
LWSLGSKTLFYFIFTTIVSVSIGISLTTVTKPGYQIQSKIQTQTITNPMSTLEKKEKEESIPEIIANIIPKNIVNVWSKQ
QMLSIVFLGMILGVFFLTSKESGTALKAFCHSLESFCLWVVSTAMKLAPLAVFGLMSYAMIQIGFSLVFGLLTYILTVLG
GLCLILVFYSFMVFFFTKKNPFLFFNQIREIPILGFSTSSSSSVLPYSLKIAKEKLRLKESVADFVLPLGATINMDGTAL
YQAVATVFLSQVYQIELSSVELFLLAGTVTAASIGTAATPGVGLVILSSILYTFHIPIEGITILFGVDRFLDMCRTSVNL
TGDLSCAFIMDHIWKEEKQL
>Mature_419_residues
SLPKIAFWKQILISLVLGLALGILLNPETGFITSDAINPYLSWLKLPGDLFLNLLQMIMIPLVIVSIALGVSSLKNLKDL
WSLGSKTLFYFIFTTIVSVSIGISLTTVTKPGYQIQSKIQTQTITNPMSTLEKKEKEESIPEIIANIIPKNIVNVWSKQQ
MLSIVFLGMILGVFFLTSKESGTALKAFCHSLESFCLWVVSTAMKLAPLAVFGLMSYAMIQIGFSLVFGLLTYILTVLGG
LCLILVFYSFMVFFFTKKNPFLFFNQIREIPILGFSTSSSSSVLPYSLKIAKEKLRLKESVADFVLPLGATINMDGTALY
QAVATVFLSQVYQIELSSVELFLLAGTVTAASIGTAATPGVGLVILSSILYTFHIPIEGITILFGVDRFLDMCRTSVNLT
GDLSCAFIMDHIWKEEKQL

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI194239697, Length=465, Percent_Identity=29.8924731182796, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI4827012, Length=456, Percent_Identity=29.3859649122807, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI169790839, Length=448, Percent_Identity=29.0178571428571, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI66773030, Length=450, Percent_Identity=29.3333333333333, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI40254478, Length=421, Percent_Identity=29.2161520190024, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI21314632, Length=421, Percent_Identity=28.5035629453682, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5032093, Length=414, Percent_Identity=29.4685990338164, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI223468564, Length=263, Percent_Identity=31.9391634980989, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI223468566, Length=263, Percent_Identity=31.9391634980989, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI262359914, Length=389, Percent_Identity=27.2493573264781, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI301601644, Length=153, Percent_Identity=33.9869281045752, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1790514, Length=424, Percent_Identity=31.3679245283019, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789947, Length=427, Percent_Identity=25.7611241217799, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788024, Length=397, Percent_Identity=24.4332493702771, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789473, Length=231, Percent_Identity=25.1082251082251, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71996953, Length=466, Percent_Identity=29.3991416309013, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537407, Length=439, Percent_Identity=28.4738041002278, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71983099, Length=420, Percent_Identity=28.8095238095238, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71983106, Length=420, Percent_Identity=28.8095238095238, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI193206505, Length=438, Percent_Identity=28.7671232876712, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI193206654, Length=451, Percent_Identity=28.6031042128603, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17541374, Length=419, Percent_Identity=28.8782816229117, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24583025, Length=443, Percent_Identity=27.313769751693, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17137668, Length=443, Percent_Identity=27.313769751693, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24583023, Length=443, Percent_Identity=27.313769751693, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI281360483, Length=459, Percent_Identity=28.322440087146, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137666, Length=459, Percent_Identity=28.322440087146, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281360481, Length=459, Percent_Identity=28.322440087146, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46252; Mature: 46121

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPKIAFWKQILISLVLGLALGILLNPETGFITSDAINPYLSWLKLPGDLFLNLLQMIM
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHH
IPLVIVSIALGVSSLKNLKDLWSLGSKTLFYFIFTTIVSVSIGISLTTVTKPGYQIQSKI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHH
QTQTITNPMSTLEKKEKEESIPEIIANIIPKNIVNVWSKQQMLSIVFLGMILGVFFLTSK
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESGTALKAFCHSLESFCLWVVSTAMKLAPLAVFGLMSYAMIQIGFSLVFGLLTYILTVLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLCLILVFYSFMVFFFTKKNPFLFFNQIREIPILGFSTSSSSSVLPYSLKIAKEKLRLKE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
SVADFVLPLGATINMDGTALYQAVATVFLSQVYQIELSSVELFLLAGTVTAASIGTAATP
HHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHCCCCCCC
GVGLVILSSILYTFHIPIEGITILFGVDRFLDMCRTSVNLTGDLSCAFIMDHIWKEEKQL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLPKIAFWKQILISLVLGLALGILLNPETGFITSDAINPYLSWLKLPGDLFLNLLQMIM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHH
IPLVIVSIALGVSSLKNLKDLWSLGSKTLFYFIFTTIVSVSIGISLTTVTKPGYQIQSKI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHH
QTQTITNPMSTLEKKEKEESIPEIIANIIPKNIVNVWSKQQMLSIVFLGMILGVFFLTSK
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESGTALKAFCHSLESFCLWVVSTAMKLAPLAVFGLMSYAMIQIGFSLVFGLLTYILTVLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLCLILVFYSFMVFFFTKKNPFLFFNQIREIPILGFSTSSSSSVLPYSLKIAKEKLRLKE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
SVADFVLPLGATINMDGTALYQAVATVFLSQVYQIELSSVELFLLAGTVTAASIGTAATP
HHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHCCCCCCC
GVGLVILSSILYTFHIPIEGITILFGVDRFLDMCRTSVNLTGDLSCAFIMDHIWKEEKQL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]