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The map label for this gene is oprM [H]

Identifier: 183222734

GI number: 183222734

Start: 3513723

End: 3515150

Strand: Direct

Name: oprM [H]

Synonym: LEPBI_I3390

Alternate gene names: 183222734

Gene position: 3513723-3515150 (Clockwise)

Preceding gene: 183222733

Following gene: 183222751

Centisome position: 97.61

GC content: 36.62

Gene sequence:

>1428_bases
ATGAAACGGATCATCATCACATTCATAGTTTTATGGAATATTTCCTGTATTCCCGCGCTTTACGAAAGAGATAAGGAAGA
TTTACAACTTCCAGAACAATTTCAAAATTGGGAGTCCTCTGAAAAAGCGATTAAGTTACAAACTGAAATTTGGAACCAAT
TTTTTAATGAACCACAATTGATTTCATTGATTGAGATTGCTATAAAAAACAATCAGGAACTTGAAGTTCTAGAACAGGAA
ATCAGTATCGCAAATAACGAAGTTTTTGCAAGGCAAGGGGAATACTTACCAAAATTATCTTTACAAGCCGATGTTGGAAG
CGAACAGAAAGAAAGATTTAGTACACCTAACGCAAATTCTCCGACTTTGTTTGCACACGGTGGACTCGTTATGAGTTGGG
AAATTGACATTTGGAAAAAACTAAGAAACGCTACAAAATCTGCATACCTTAGATATTTAGCAAGTATAGAAGGAAAACGT
TTTGTTGTGACCAACTTAGTTGCAGAAATTTCGGATACGTATTTTGAATTGAAGGCACTCGACAACCAACTCACCCTCAT
TCAAAACTATATCGAAGTTTTATCCCAAGTAAAGGAAATCGTCGTATTACAAAGAGAAGCTGGTAGAACCACTTCTCTTG
CTGTCAAACGATTCGAAGCCGAGGTTTCCAAAAACCTAGCACGTAAATACGATATCGTTCAAAGGATTGCAATCACTGAA
AATCGACTTAATTTCTTATTAGGAAGGTTTCCAGAAAAAATCAACCGTACTTCAGATGATTTTTTAGAAATCACACTCCC
TGAAATCCAAAAATCTGTTCCCATTGATCTTCTTGAGAATCGACCCGATATCAAACAAGCCACTCTGATTTTAGAATCTC
GAAAATTGGATATCGAAGTGGCTAGAGCAAGATTTTATCCATCACTTTCCATTGATGGCAATATTGGTTATGAAGTGTTT
AATTCCAAACATTTTAAAGGAACACCAGTTTCGCTCGCTTATGGATTAGGTGGAGGGATCATCGCACCACTCATCAACAG
AAAAGCAATAGAAGCCAATTATGCAACGGCAAATAACTTACAAATCCAAGCCGTTTACAATTATGAAGTCTCACTCCTAA
AAGCCTTTACAGAAGTGACCAATCAAATTGTAAAAATCAAAAATCTCTCACAGAAGTTTGAAGCAAAAAACAAACAGGTG
CAGAATTTAAAAGAATCAGTAGAAATCTCCAATATCCTCTTCAAAGCAGGAAGGATAGACTACATTGATGTTTTGTTTAG
CCAGAGGGATTTTCTGGAAGCACAAGTGGAAGCCTTTGAACTAAAGTACAGTTTACTTGAATCTAATATTGGTTTGTATA
AAGCACTCGGTGGTGGTTGGCGAGGCCAAAACGAACAAGACAGCGAACGAAAGAACGGGACATTTTAA

Upstream 100 bases:

>100_bases
TTTACCACGAGGACCATATGCCACTTTCGGAATCGGACGTGAGTTACCAGAATTCAGAAATCGAACGTAAAAAAAATAGA
AAGACAAGGAAGGAAATCAA

Downstream 100 bases:

>100_bases
TTATATCCCCGCTTTCCACATCCATCGGCGGCGCGGGGAATTTTTTTAATGTCAGAAATTGCAATCACCAACTCACTTTA
CAAAGAAAGTTTTGGTATCA

Product: ABC transporter outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKRIIITFIVLWNISCIPALYERDKEDLQLPEQFQNWESSEKAIKLQTEIWNQFFNEPQLISLIEIAIKNNQELEVLEQE
ISIANNEVFARQGEYLPKLSLQADVGSEQKERFSTPNANSPTLFAHGGLVMSWEIDIWKKLRNATKSAYLRYLASIEGKR
FVVTNLVAEISDTYFELKALDNQLTLIQNYIEVLSQVKEIVVLQREAGRTTSLAVKRFEAEVSKNLARKYDIVQRIAITE
NRLNFLLGRFPEKINRTSDDFLEITLPEIQKSVPIDLLENRPDIKQATLILESRKLDIEVARARFYPSLSIDGNIGYEVF
NSKHFKGTPVSLAYGLGGGIIAPLINRKAIEANYATANNLQIQAVYNYEVSLLKAFTEVTNQIVKIKNLSQKFEAKNKQV
QNLKESVEISNILFKAGRIDYIDVLFSQRDFLEAQVEAFELKYSLLESNIGLYKALGGGWRGQNEQDSERKNGTF

Sequences:

>Translated_475_residues
MKRIIITFIVLWNISCIPALYERDKEDLQLPEQFQNWESSEKAIKLQTEIWNQFFNEPQLISLIEIAIKNNQELEVLEQE
ISIANNEVFARQGEYLPKLSLQADVGSEQKERFSTPNANSPTLFAHGGLVMSWEIDIWKKLRNATKSAYLRYLASIEGKR
FVVTNLVAEISDTYFELKALDNQLTLIQNYIEVLSQVKEIVVLQREAGRTTSLAVKRFEAEVSKNLARKYDIVQRIAITE
NRLNFLLGRFPEKINRTSDDFLEITLPEIQKSVPIDLLENRPDIKQATLILESRKLDIEVARARFYPSLSIDGNIGYEVF
NSKHFKGTPVSLAYGLGGGIIAPLINRKAIEANYATANNLQIQAVYNYEVSLLKAFTEVTNQIVKIKNLSQKFEAKNKQV
QNLKESVEISNILFKAGRIDYIDVLFSQRDFLEAQVEAFELKYSLLESNIGLYKALGGGWRGQNEQDSERKNGTF
>Mature_475_residues
MKRIIITFIVLWNISCIPALYERDKEDLQLPEQFQNWESSEKAIKLQTEIWNQFFNEPQLISLIEIAIKNNQELEVLEQE
ISIANNEVFARQGEYLPKLSLQADVGSEQKERFSTPNANSPTLFAHGGLVMSWEIDIWKKLRNATKSAYLRYLASIEGKR
FVVTNLVAEISDTYFELKALDNQLTLIQNYIEVLSQVKEIVVLQREAGRTTSLAVKRFEAEVSKNLARKYDIVQRIAITE
NRLNFLLGRFPEKINRTSDDFLEITLPEIQKSVPIDLLENRPDIKQATLILESRKLDIEVARARFYPSLSIDGNIGYEVF
NSKHFKGTPVSLAYGLGGGIIAPLINRKAIEANYATANNLQIQAVYNYEVSLLKAFTEVTNQIVKIKNLSQKFEAKNKQV
QNLKESVEISNILFKAGRIDYIDVLFSQRDFLEAQVEAFELKYSLLESNIGLYKALGGGWRGQNEQDSERKNGTF

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=25, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI2367348, Length=456, Percent_Identity=24.5614035087719, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54376; Mature: 54376

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIIITFIVLWNISCIPALYERDKEDLQLPEQFQNWESSEKAIKLQTEIWNQFFNEPQL
CCHHEEEHHHHHCCHHHHHHHHCCHHHCCCCHHHCCCCCCCHHEEHHHHHHHHHCCCCHH
ISLIEIAIKNNQELEVLEQEISIANNEVFARQGEYLPKLSLQADVGSEQKERFSTPNANS
HHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCCCCHHHHHCCCCCCCC
PTLFAHGGLVMSWEIDIWKKLRNATKSAYLRYLASIEGKRFVVTNLVAEISDTYFELKAL
CEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
DNQLTLIQNYIEVLSQVKEIVVLQREAGRTTSLAVKRFEAEVSKNLARKYDIVQRIAITE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLNFLLGRFPEKINRTSDDFLEITLPEIQKSVPIDLLENRPDIKQATLILESRKLDIEV
HHHHHHHHHCHHHHCCCCCCEEEEEHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCEE
ARARFYPSLSIDGNIGYEVFNSKHFKGTPVSLAYGLGGGIIAPLINRKAIEANYATANNL
HHHHCCCCEEECCCCCHHHHCCCCCCCCCCEEEECCCCHHHHHHHCCHHEECCCCCCCCE
QIQAVYNYEVSLLKAFTEVTNQIVKIKNLSQKFEAKNKQVQNLKESVEISNILFKAGRID
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
YIDVLFSQRDFLEAQVEAFELKYSLLESNIGLYKALGGGWRGQNEQDSERKNGTF
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRIIITFIVLWNISCIPALYERDKEDLQLPEQFQNWESSEKAIKLQTEIWNQFFNEPQL
CCHHEEEHHHHHCCHHHHHHHHCCHHHCCCCHHHCCCCCCCHHEEHHHHHHHHHCCCCHH
ISLIEIAIKNNQELEVLEQEISIANNEVFARQGEYLPKLSLQADVGSEQKERFSTPNANS
HHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCCCCHHHHHCCCCCCCC
PTLFAHGGLVMSWEIDIWKKLRNATKSAYLRYLASIEGKRFVVTNLVAEISDTYFELKAL
CEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
DNQLTLIQNYIEVLSQVKEIVVLQREAGRTTSLAVKRFEAEVSKNLARKYDIVQRIAITE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRLNFLLGRFPEKINRTSDDFLEITLPEIQKSVPIDLLENRPDIKQATLILESRKLDIEV
HHHHHHHHHCHHHHCCCCCCEEEEEHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCEE
ARARFYPSLSIDGNIGYEVFNSKHFKGTPVSLAYGLGGGIIAPLINRKAIEANYATANNL
HHHHCCCCEEECCCCCHHHHCCCCCCCCCCEEEECCCCHHHHHHHCCHHEECCCCCCCCE
QIQAVYNYEVSLLKAFTEVTNQIVKIKNLSQKFEAKNKQVQNLKESVEISNILFKAGRID
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
YIDVLFSQRDFLEAQVEAFELKYSLLESNIGLYKALGGGWRGQNEQDSERKNGTF
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]