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The map label for this gene is ruvC [H]

Identifier: 183222663

GI number: 183222663

Start: 3431590

End: 3432066

Strand: Direct

Name: ruvC [H]

Synonym: LEPBI_I3318

Alternate gene names: 183222663

Gene position: 3431590-3432066 (Clockwise)

Preceding gene: 183222658

Following gene: 183222665

Centisome position: 95.33

GC content: 48.43

Gene sequence:

>477_bases
TTGAAAATCATTGGAATTGATCCAGGGTCCCACCGTGTCGGGTATGCCGTTTTGTCTTTCCCTGAAGGAATGCGGCGCAA
TCCAGAACTTCTAGCCTATGGAACAATTGAAGTGGCTCCGAAAACCCCTTCTCCCGTCAATTTATTGCAAATTCGTTCCG
AACTTCAAAAAATCCTCTCCCTTTACCAACCAGAACACGCCGCCGTGGAGGAGTTATTCTTTGTCCAAAACACAACCACG
GGGATGAAGGTTTCTGAATCACGAGGGGTGATTTTACTCACTCTGGGCGAAAACCAAATCCCGATGGTGTCACTCACTGC
CACACAAATCAAAAAAGGAATCTCTGCCAAAGGGAACGCCACCAAAAAGGAAGTCCGGGCGGCGATCCAAATGATTTTGG
GATTTAAGGACCTAAAAGGCCACGATGACTCCTGGGATGCCATCGCGTGTGCCTTTGTCGGTCGGTCCTTAGTTTGA

Upstream 100 bases:

>100_bases
TATACGGAAGGAAAGTACCCTGTCATTTTCATTTTTAGACTAGGTTTTGGGTTGAGCCTTTCGAAAAATGTCCCTAAGAA
TGAAATTAGGAAATAAATGA

Downstream 100 bases:

>100_bases
GGAACTACGAGCCAAGTTCATTGACAAAAAGATCTCATTCTTTTGTTCCCGACTGGCATTGGCTGTGATTGTAGAATATC
CAGTTGTGATTTCCAGTTCT

Product: putative crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKIIGIDPGSHRVGYAVLSFPEGMRRNPELLAYGTIEVAPKTPSPVNLLQIRSELQKILSLYQPEHAAVEELFFVQNTTT
GMKVSESRGVILLTLGENQIPMVSLTATQIKKGISAKGNATKKEVRAAIQMILGFKDLKGHDDSWDAIACAFVGRSLV

Sequences:

>Translated_158_residues
MKIIGIDPGSHRVGYAVLSFPEGMRRNPELLAYGTIEVAPKTPSPVNLLQIRSELQKILSLYQPEHAAVEELFFVQNTTT
GMKVSESRGVILLTLGENQIPMVSLTATQIKKGISAKGNATKKEVRAAIQMILGFKDLKGHDDSWDAIACAFVGRSLV
>Mature_158_residues
MKIIGIDPGSHRVGYAVLSFPEGMRRNPELLAYGTIEVAPKTPSPVNLLQIRSELQKILSLYQPEHAAVEELFFVQNTTT
GMKVSESRGVILLTLGENQIPMVSLTATQIKKGISAKGNATKKEVRAAIQMILGFKDLKGHDDSWDAIACAFVGRSLV

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17219; Mature: 17219

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIGIDPGSHRVGYAVLSFPEGMRRNPELLAYGTIEVAPKTPSPVNLLQIRSELQKILS
CEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
LYQPEHAAVEELFFVQNTTTGMKVSESRGVILLTLGENQIPMVSLTATQIKKGISAKGNA
HHCCCHHHHHHHHHEECCCCCCEEECCCCEEEEEECCCCCCEEEEEHHHHHHCCCCCCCC
TKKEVRAAIQMILGFKDLKGHDDSWDAIACAFVGRSLV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIIGIDPGSHRVGYAVLSFPEGMRRNPELLAYGTIEVAPKTPSPVNLLQIRSELQKILS
CEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
LYQPEHAAVEELFFVQNTTTGMKVSESRGVILLTLGENQIPMVSLTATQIKKGISAKGNA
HHCCCHHHHHHHHHEECCCCCCEEECCCCEEEEEECCCCCCEEEEEHHHHHHCCCCCCCC
TKKEVRAAIQMILGFKDLKGHDDSWDAIACAFVGRSLV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA