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The map label for this gene is ytpA [H]

Identifier: 183222509

GI number: 183222509

Start: 3278697

End: 3279572

Strand: Direct

Name: ytpA [H]

Synonym: LEPBI_I3164

Alternate gene names: 183222509

Gene position: 3278697-3279572 (Clockwise)

Preceding gene: 183222506

Following gene: 183222510

Centisome position: 91.08

GC content: 43.26

Gene sequence:

>876_bases
TTGACATCTATGAGTAATTGGGAACAAGCCTACTCCCGCGTGGAGTCTACCTTTCAAAACAAAGATGGTGGTAAAATTTA
TTACCAGATTTACCGACCAAAGTCAGGAGTCAAACGAGTGCTCGTTGTCCACCACGGGATTGGGGAACACGGCGGTCGTT
ACAATTTTTTGTTGGAAGCTATGGCGGAACGTAACTATGCCATTTACCTCATCGACTGCCGAGGCCATGGTAAGTCCGAT
GGTCGCCGCGGGGTCATCACCCATTTTTCTGATTTTTTTGCCGACTTAAAACAACTCATCGACATCGCCAAACAAAACGA
AGGTGTTAGTAAGGTTACCTTACTTGGTCACTCCATGGGAGCTGCGATTACTTTCTTGTATACAGCTACCGACAACTACC
AAAATGATTTGGATGCTTATATCTGTAGCGCCCTACCCATCAAAGTGAAAACAGATCTTGTGATGGACATAAAAAAAGCA
GCCGGTGGTTTTTTAGCAAAAGCCCTCCCCACTCTCACGATTCCAACAGGTCTCAACGTAAATCTCATCTCTCGTGACAA
ATCGGTGGTTGACGCCTATGTCAAAGACCCACTTGTGCACGGTAATGTTTGTGCGTATCTGGGAGATTACCTTCTCAATT
GTTACACACTCGCATTGGAATCTGCTGAAAAAATCAAAGTGCCAATTTATATGTTCCATGGAAAAGAAGACCAAATTGCT
CTACCTGAAGGCACAAACGATGCATTTGAACGAGTTGCTTCCAAAGACAAAACCAAAAGACTTTTTGATGAATTATACCA
TGAAACCATGAACGAACTTCCAAAAGACAGAGCAGTCGTCTTAAATGAGTTAGTTGCTTGGATCGACAAACACTAA

Upstream 100 bases:

>100_bases
GGGGCGAAGGGAAGGATCTTTCGCATTTGGAACCAGTTTGCAAAACAGGTTTCCCAAGAATAGGAAAAGTTGTTTCTTTT
TAGGTTTCCATCGTCCATCC

Downstream 100 bases:

>100_bases
GGAGAAAATGCCAATGGCAATAACAAAAGATATAGTTGGAAAAAAACTAGATCGTTTTGATTTCACAGTGGAACGAGGAA
AGATCAAAGAATTTTGCCTC

Product: putative acylglycerol lipase

Products: glycerophosphocholine; carboxylate

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTSMSNWEQAYSRVESTFQNKDGGKIYYQIYRPKSGVKRVLVVHHGIGEHGGRYNFLLEAMAERNYAIYLIDCRGHGKSD
GRRGVITHFSDFFADLKQLIDIAKQNEGVSKVTLLGHSMGAAITFLYTATDNYQNDLDAYICSALPIKVKTDLVMDIKKA
AGGFLAKALPTLTIPTGLNVNLISRDKSVVDAYVKDPLVHGNVCAYLGDYLLNCYTLALESAEKIKVPIYMFHGKEDQIA
LPEGTNDAFERVASKDKTKRLFDELYHETMNELPKDRAVVLNELVAWIDKH

Sequences:

>Translated_291_residues
MTSMSNWEQAYSRVESTFQNKDGGKIYYQIYRPKSGVKRVLVVHHGIGEHGGRYNFLLEAMAERNYAIYLIDCRGHGKSD
GRRGVITHFSDFFADLKQLIDIAKQNEGVSKVTLLGHSMGAAITFLYTATDNYQNDLDAYICSALPIKVKTDLVMDIKKA
AGGFLAKALPTLTIPTGLNVNLISRDKSVVDAYVKDPLVHGNVCAYLGDYLLNCYTLALESAEKIKVPIYMFHGKEDQIA
LPEGTNDAFERVASKDKTKRLFDELYHETMNELPKDRAVVLNELVAWIDKH
>Mature_290_residues
TSMSNWEQAYSRVESTFQNKDGGKIYYQIYRPKSGVKRVLVVHHGIGEHGGRYNFLLEAMAERNYAIYLIDCRGHGKSDG
RRGVITHFSDFFADLKQLIDIAKQNEGVSKVTLLGHSMGAAITFLYTATDNYQNDLDAYICSALPIKVKTDLVMDIKKAA
GGFLAKALPTLTIPTGLNVNLISRDKSVVDAYVKDPLVHGNVCAYLGDYLLNCYTLALESAEKIKVPIYMFHGKEDQIAL
PEGTNDAFERVASKDKTKRLFDELYHETMNELPKDRAVVLNELVAWIDKH

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI6005786, Length=272, Percent_Identity=29.7794117647059, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI51242953, Length=272, Percent_Identity=29.7794117647059, Blast_Score=124, Evalue=7e-29,
Organism=Escherichia coli, GI1787678, Length=282, Percent_Identity=23.0496453900709, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322756, Length=296, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: 3.1.1.5

Molecular weight: Translated: 32663; Mature: 32532

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00120 LIPASE_SER ; PS00697 DNA_LIGASE_A1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMSNWEQAYSRVESTFQNKDGGKIYYQIYRPKSGVKRVLVVHHGIGEHGGRYNFLLEA
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHEEEEEECCCCCCCCHHHHHHHH
MAERNYAIYLIDCRGHGKSDGRRGVITHFSDFFADLKQLIDIAKQNEGVSKVTLLGHSMG
HHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHC
AAITFLYTATDNYQNDLDAYICSALPIKVKTDLVMDIKKAAGGFLAKALPTLTIPTGLNV
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCHHHHHHCCCEECCCCCCE
NLISRDKSVVDAYVKDPLVHGNVCAYLGDYLLNCYTLALESAEKIKVPIYMFHGKEDQIA
EEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
LPEGTNDAFERVASKDKTKRLFDELYHETMNELPKDRAVVLNELVAWIDKH
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSMSNWEQAYSRVESTFQNKDGGKIYYQIYRPKSGVKRVLVVHHGIGEHGGRYNFLLEA
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHEEEEEECCCCCCCCHHHHHHHH
MAERNYAIYLIDCRGHGKSDGRRGVITHFSDFFADLKQLIDIAKQNEGVSKVTLLGHSMG
HHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHC
AAITFLYTATDNYQNDLDAYICSALPIKVKTDLVMDIKKAAGGFLAKALPTLTIPTGLNV
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCHHHHHHCCCEECCCCCCE
NLISRDKSVVDAYVKDPLVHGNVCAYLGDYLLNCYTLALESAEKIKVPIYMFHGKEDQIA
EEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
LPEGTNDAFERVASKDKTKRLFDELYHETMNELPKDRAVVLNELVAWIDKH
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-lysophosphatidylcholine; H2O

Specific reaction: 2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]