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The map label for this gene is metZ [H]

Identifier: 183221928

GI number: 183221928

Start: 2654066

End: 2655250

Strand: Reverse

Name: metZ [H]

Synonym: LEPBI_I2567

Alternate gene names: 183221928

Gene position: 2655250-2654066 (Counterclockwise)

Preceding gene: 183221929

Following gene: 183221926

Centisome position: 73.76

GC content: 41.69

Gene sequence:

>1185_bases
TTGGTAACAGACATGTTTGAACATTTTGAAACTGAAGCCATCCGCATCCAAACCAAACGCACTGGGGAAAAAGAACACTC
TACCCCATTATTTTTAACCTCCAGTTTTGTTTTTGATGATGCAGAACATGCGAGGGCTCTTTTTGCGGAAGAAGTCACGG
GAAACCAATACACAAGGTTTTCCAACCCTAATACTACAGAACTCATTGATAAACTTTGTGCATTGGAACAAACCGAAGAT
GGAATTGCAACTGCCTCTGGGATGTCCGCCGTATTTACCTCAATCTTTGGTCTTGTGAAATCAGGAGATCATATTGTATC
TGCAAGAGCTATCTTTGGATCCACTCACCAAATTTTTGCCAACATCTTACCACGGTTTGGTGTAACAACTACCTATGTTG
ATATCGCCAAACCAGAAGAATGGGAAAAGGCCTTTCAAGAGAATACAAAAATAGTTTATATCGAAACACCATCGAATCCT
GGACTTGATATTGTGGATTTGGAATGGGTATCTGCTTTATGCAAAAAGAAAAAAGCCATCCTCATTGTAGACAATTGTTT
TTGTTCTCCTTATATCCAACGTCCTGCTGACTTTGGCGCTGACGTGGTGATCCATTCTGCCACAAAGTACTTAGATGGCC
AAGGAAGAGTGATTGCTGGTGTGATTTTAGGGAAAAAAGAATTCATCCAACCCATTCGTTATATGGCAAGAAATACGGGT
CCTTCCCTTTCTCCAATGAACGCGTGGATCATTTCCAAAAGTTTGGAAACATTGGCAGTACGGATGGACCGCCATGCCGA
AAATGCAATGAAACTTGCAACATTCTTAAGTGAATCAAAGGATGTAGAACTAGTTCGATATCCGTTTTTGCCAACTGATC
CAGGTTATGCGATTGCTAAAAAACAAATGCGCTCGGGTGGAGGTATCGTTTCTTTTGTGATCAAAGGTGGCGTGGAACGA
GCAAAAAAATTCTTAGATGCACTCAAGTGGTTTTCCTTAACTGCAAATTTAGGGGATACAAGAACAACTGTCACTCATCC
AACAACCACAACACATTCCAAACTCACCGAAGCGGAACGATTGGCTGTGGGGATTTTACCGGGTCTCATTCGTGTGTCCG
TTGGACTAGAGCACATAGATGATATTGTTGCGGAAGTGAAACAGGCATTGGCAAACTCAAAGTAA

Upstream 100 bases:

>100_bases
TGCGGAAATTTCATAAAATATCTGCTATAGTAGTCTAAATCTGATTCTATAGCAGATATTTTCCAAAAAAAATGATGAAT
TCGTATGCTCATCAACAAGT

Downstream 100 bases:

>100_bases
ATAGGTCTCACATGTGAGCGTTTCTATTTGTTAAAATTCAAATTGTTACAACGCTCACAAACTTCTTCTGGAATCACTCC
TACTTTCATGAGTAATCCAA

Product: putative Cys/Met metabolism PLP-dependent enzyme

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MVTDMFEHFETEAIRIQTKRTGEKEHSTPLFLTSSFVFDDAEHARALFAEEVTGNQYTRFSNPNTTELIDKLCALEQTED
GIATASGMSAVFTSIFGLVKSGDHIVSARAIFGSTHQIFANILPRFGVTTTYVDIAKPEEWEKAFQENTKIVYIETPSNP
GLDIVDLEWVSALCKKKKAILIVDNCFCSPYIQRPADFGADVVIHSATKYLDGQGRVIAGVILGKKEFIQPIRYMARNTG
PSLSPMNAWIISKSLETLAVRMDRHAENAMKLATFLSESKDVELVRYPFLPTDPGYAIAKKQMRSGGGIVSFVIKGGVER
AKKFLDALKWFSLTANLGDTRTTVTHPTTTTHSKLTEAERLAVGILPGLIRVSVGLEHIDDIVAEVKQALANSK

Sequences:

>Translated_394_residues
MVTDMFEHFETEAIRIQTKRTGEKEHSTPLFLTSSFVFDDAEHARALFAEEVTGNQYTRFSNPNTTELIDKLCALEQTED
GIATASGMSAVFTSIFGLVKSGDHIVSARAIFGSTHQIFANILPRFGVTTTYVDIAKPEEWEKAFQENTKIVYIETPSNP
GLDIVDLEWVSALCKKKKAILIVDNCFCSPYIQRPADFGADVVIHSATKYLDGQGRVIAGVILGKKEFIQPIRYMARNTG
PSLSPMNAWIISKSLETLAVRMDRHAENAMKLATFLSESKDVELVRYPFLPTDPGYAIAKKQMRSGGGIVSFVIKGGVER
AKKFLDALKWFSLTANLGDTRTTVTHPTTTTHSKLTEAERLAVGILPGLIRVSVGLEHIDDIVAEVKQALANSK
>Mature_394_residues
MVTDMFEHFETEAIRIQTKRTGEKEHSTPLFLTSSFVFDDAEHARALFAEEVTGNQYTRFSNPNTTELIDKLCALEQTED
GIATASGMSAVFTSIFGLVKSGDHIVSARAIFGSTHQIFANILPRFGVTTTYVDIAKPEEWEKAFQENTKIVYIETPSNP
GLDIVDLEWVSALCKKKKAILIVDNCFCSPYIQRPADFGADVVIHSATKYLDGQGRVIAGVILGKKEFIQPIRYMARNTG
PSLSPMNAWIISKSLETLAVRMDRHAENAMKLATFLSESKDVELVRYPFLPTDPGYAIAKKQMRSGGGIVSFVIKGGVER
AKKFLDALKWFSLTANLGDTRTTVTHPTTTTHSKLTEAERLAVGILPGLIRVSVGLEHIDDIVAEVKQALANSK

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=34.4473007712082, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI299473759, Length=278, Percent_Identity=37.410071942446, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=29.3814432989691, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1790375, Length=362, Percent_Identity=30.3867403314917, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789383, Length=371, Percent_Identity=28.3018867924528, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17538055, Length=391, Percent_Identity=36.3171355498721, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17560056, Length=391, Percent_Identity=34.2710997442455, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=32.520325203252, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=343, Percent_Identity=39.067055393586, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=34.4827586206897, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321254, Length=394, Percent_Identity=26.9035532994924, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=380, Percent_Identity=28.1578947368421, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=293, Percent_Identity=24.9146757679181, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322970, Length=391, Percent_Identity=25.0639386189258, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19922554, Length=365, Percent_Identity=33.4246575342466, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43494; Mature: 43494

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTDMFEHFETEAIRIQTKRTGEKEHSTPLFLTSSFVFDDAEHARALFAEEVTGNQYTRF
CCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCEECCHHHHHHHHHHHHCCCCEEEC
SNPNTTELIDKLCALEQTEDGIATASGMSAVFTSIFGLVKSGDHIVSARAIFGSTHQIFA
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHH
NILPRFGVTTTYVDIAKPEEWEKAFQENTKIVYIETPSNPGLDIVDLEWVSALCKKKKAI
HHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHHHCCCCEE
LIVDNCFCSPYIQRPADFGADVVIHSATKYLDGQGRVIAGVILGKKEFIQPIRYMARNTG
EEEECCCCCHHHCCCCCCCCCEEEECHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCC
PSLSPMNAWIISKSLETLAVRMDRHAENAMKLATFLSESKDVELVRYPFLPTDPGYAIAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHH
KQMRSGGGIVSFVIKGGVERAKKFLDALKWFSLTANLGDTRTTVTHPTTTTHSKLTEAER
HHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHH
LAVGILPGLIRVSVGLEHIDDIVAEVKQALANSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVTDMFEHFETEAIRIQTKRTGEKEHSTPLFLTSSFVFDDAEHARALFAEEVTGNQYTRF
CCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCEECCHHHHHHHHHHHHCCCCEEEC
SNPNTTELIDKLCALEQTEDGIATASGMSAVFTSIFGLVKSGDHIVSARAIFGSTHQIFA
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHH
NILPRFGVTTTYVDIAKPEEWEKAFQENTKIVYIETPSNPGLDIVDLEWVSALCKKKKAI
HHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHHHCCCCEE
LIVDNCFCSPYIQRPADFGADVVIHSATKYLDGQGRVIAGVILGKKEFIQPIRYMARNTG
EEEECCCCCHHHCCCCCCCCCEEEECHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCC
PSLSPMNAWIISKSLETLAVRMDRHAENAMKLATFLSESKDVELVRYPFLPTDPGYAIAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHH
KQMRSGGGIVSFVIKGGVERAKKFLDALKWFSLTANLGDTRTTVTHPTTTTHSKLTEAER
HHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHH
LAVGILPGLIRVSVGLEHIDDIVAEVKQALANSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]