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The map label for this gene is rhdA [H]

Identifier: 183221926

GI number: 183221926

Start: 2652529

End: 2653425

Strand: Reverse

Name: rhdA [H]

Synonym: LEPBI_I2565

Alternate gene names: 183221926

Gene position: 2653425-2652529 (Counterclockwise)

Preceding gene: 183221928

Following gene: 183221925

Centisome position: 73.71

GC content: 40.8

Gene sequence:

>897_bases
GTGAAAATCCAAAGAAGCTACGGAATCTACCTATTCGCCATCGCCTTGTTTTGGGCTCTTTGGCTCCAATTAAGTGCCGG
TCCTAAAAAACAAGGTCTGAACCCAAAGACCCATACTTGGTTTCTCTCCCCAAAAGAAGCCCTGGACTTACCAGAATTTA
AAACCATCGATACAAGGTCTTTTCCTTCTAGGCTCTTAGAAAAACTTCCAATGTCCCAAGTCCTCGGTTGGGAAGACCTT
TCTCTCAAAGAAAATCCTTTCCGTGGAAAATTACAAAAAGAAGAGGATATCCAAAAAAAACTTTTGAGTTTGGGCTTCCA
CTTGGAGGATTTGATCCTCGTGTTAGGTGATGGAGCCAGTGGATGGGGTGAAGAGGGGAGAATTGTTTGGAGTTTACGGG
AAGTTGGATTTTCCAATGTGTTTTGGTTTGATGGTAACGTCAGATCATTAAAAGAGGTACTTGTGGTTCGCAAACAAAGG
GCATCCCATCCAATAGAAAAGGATACAATCCAAATCAAAACAAATATCACAAAGGAAGAAATTTCCAAACAGTTACAAAA
TAATTTTTACCAAATCTTAGATACGCGCGAGACAAGGGAATTTTCCGGCTCTACTCCCTACGGCGAATCACGAGGGGGGC
ATATCCCCGGCGCAAAATCATTCTATTACCAAAATCTATTTGATGCGAAAGGAAGTATCAAATCAAAACAAGAAGTAGAA
ACAATCCTATCTCAGTTGGGTATTACAAAGACAAAACCCATCATTGCTTATTGTACAGGTGGTGTTCGCTCTGCTTTTGT
GGTAGGAATCCTTCGATCTTATGGTTATAATGCTTATAACTATTCTGGATCGATGTGGGAATGGTCTTTTTACCCAGACC
TTCCCATGGAAAAATAA

Upstream 100 bases:

>100_bases
AGAGAGCAAGAAAAATTCGTTTTCTTGGAGGAAATGTTTATCTTATTGACGAAAATTTCGTCTTAAAAGAGTAGTTACAT
CAATTTGGGAGGGTATTTTC

Downstream 100 bases:

>100_bases
ATTTGAAACGATATTTAATACTTATATTGGTTTTGATGATGACTTTCTTAATTGGAGTGTATCTGTTTCAGAACAAGCGA
GCCATACCCATTGAACAAGT

Product: putative thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKIQRSYGIYLFAIALFWALWLQLSAGPKKQGLNPKTHTWFLSPKEALDLPEFKTIDTRSFPSRLLEKLPMSQVLGWEDL
SLKENPFRGKLQKEEDIQKKLLSLGFHLEDLILVLGDGASGWGEEGRIVWSLREVGFSNVFWFDGNVRSLKEVLVVRKQR
ASHPIEKDTIQIKTNITKEEISKQLQNNFYQILDTRETREFSGSTPYGESRGGHIPGAKSFYYQNLFDAKGSIKSKQEVE
TILSQLGITKTKPIIAYCTGGVRSAFVVGILRSYGYNAYNYSGSMWEWSFYPDLPMEK

Sequences:

>Translated_298_residues
MKIQRSYGIYLFAIALFWALWLQLSAGPKKQGLNPKTHTWFLSPKEALDLPEFKTIDTRSFPSRLLEKLPMSQVLGWEDL
SLKENPFRGKLQKEEDIQKKLLSLGFHLEDLILVLGDGASGWGEEGRIVWSLREVGFSNVFWFDGNVRSLKEVLVVRKQR
ASHPIEKDTIQIKTNITKEEISKQLQNNFYQILDTRETREFSGSTPYGESRGGHIPGAKSFYYQNLFDAKGSIKSKQEVE
TILSQLGITKTKPIIAYCTGGVRSAFVVGILRSYGYNAYNYSGSMWEWSFYPDLPMEK
>Mature_298_residues
MKIQRSYGIYLFAIALFWALWLQLSAGPKKQGLNPKTHTWFLSPKEALDLPEFKTIDTRSFPSRLLEKLPMSQVLGWEDL
SLKENPFRGKLQKEEDIQKKLLSLGFHLEDLILVLGDGASGWGEEGRIVWSLREVGFSNVFWFDGNVRSLKEVLVVRKQR
ASHPIEKDTIQIKTNITKEEISKQLQNNFYQILDTRETREFSGSTPYGESRGGHIPGAKSFYYQNLFDAKGSIKSKQEVE
TILSQLGITKTKPIIAYCTGGVRSAFVVGILRSYGYNAYNYSGSMWEWSFYPDLPMEK

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI61835204, Length=247, Percent_Identity=23.8866396761134, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI194473681, Length=247, Percent_Identity=23.8866396761134, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI194473668, Length=247, Percent_Identity=23.8866396761134, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87082121, Length=281, Percent_Identity=25.9786476868327, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=260, Percent_Identity=24.6153846153846, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71997283, Length=276, Percent_Identity=22.1014492753623, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 34123; Mature: 34123

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQRSYGIYLFAIALFWALWLQLSAGPKKQGLNPKTHTWFLSPKEALDLPEFKTIDTRS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCHH
FPSRLLEKLPMSQVLGWEDLSLKENPFRGKLQKEEDIQKKLLSLGFHLEDLILVLGDGAS
HHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEEEECCCC
GWGEEGRIVWSLREVGFSNVFWFDGNVRSLKEVLVVRKQRASHPIEKDTIQIKTNITKEE
CCCCCCEEEEEHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHH
ISKQLQNNFYQILDTRETREFSGSTPYGESRGGHIPGAKSFYYQNLFDAKGSIKSKQEVE
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
TILSQLGITKTKPIIAYCTGGVRSAFVVGILRSYGYNAYNYSGSMWEWSFYPDLPMEK
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKIQRSYGIYLFAIALFWALWLQLSAGPKKQGLNPKTHTWFLSPKEALDLPEFKTIDTRS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCHH
FPSRLLEKLPMSQVLGWEDLSLKENPFRGKLQKEEDIQKKLLSLGFHLEDLILVLGDGAS
HHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEEEECCCC
GWGEEGRIVWSLREVGFSNVFWFDGNVRSLKEVLVVRKQRASHPIEKDTIQIKTNITKEE
CCCCCCEEEEEHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHH
ISKQLQNNFYQILDTRETREFSGSTPYGESRGGHIPGAKSFYYQNLFDAKGSIKSKQEVE
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
TILSQLGITKTKPIIAYCTGGVRSAFVVGILRSYGYNAYNYSGSMWEWSFYPDLPMEK
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]