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The map label for this gene is 183221852

Identifier: 183221852

GI number: 183221852

Start: 2566442

End: 2568610

Strand: Reverse

Name: 183221852

Synonym: LEPBI_I2485

Alternate gene names: NA

Gene position: 2568610-2566442 (Counterclockwise)

Preceding gene: 183221854

Following gene: 183221851

Centisome position: 71.36

GC content: 42.0

Gene sequence:

>2169_bases
ATGAATTCCCAAATAAGTCCCTTGGTTCGCGTCAGTGTATCCATAGTCACCTGCCTTTTTGTTTTGGCTTGTTCCGACAA
AAAACCGTCCCAGTCCAATACCGCCATACTAGGTTTAGTTGGTGCAGATCAAGTTGAAAGTTCAAAGGCAGACAATGCAG
ATCTATTGTTTAGTTCTGGTGTGAATCGCGCTGTAGCACCTAGTCTTGGTTCGTCGCCGTATTTAGTTGGTGTTGGAATT
TCTGACATCACAGGCCCTGCCGCAGAAGTAGGGATGATGGGATTTGCGGAAACCGCACAAAAGACAGAAGGGATTTATAT
GCGTTTGTGGTCTAGGGCATACATCATTGGTGATGCTTCTAAACGTGTGGTTTTTGTCAGTGCTGACCTAGGCATGATCT
TTCAATCCATCAAACAAGCAGTGAGTAAAAAAATTGCAACCGATTCCGAACTTGCTCCTTTTTATTCAGAAGCAAACGTT
TTGTTATCGGCAACACACACTCATAGTGGCCCTGGTGGGTATTCACATTACTTTTTATACAATGCGACAACTTCTGGATT
CATTAAAGAAAATTTCGATGTCATTGTGAATGGAATCTACAAATCCATTAAATTGGCGCATCAAAATTTAGTTCCTGGAA
ATGTTTATGTCAACCAAGGGGAACTAACAGATGCAAGTAAAAATCGTTCTGTAGCTGCTTATGATAAAAACCCTGCTAGT
GAACGTAATTTTTATCCATCAAATGTAGACCAAACGATGACTCTACTTAAATTAGTCGCGGCGGATGGAAGGGAATTGGG
AATGGTGAACTGGTTTGCCGTACACCCAACCAATGTTGGTCCAACTAACAAACTCATTGGGGGTGATAATAAAGGCATAG
CTTCGTATTTATTTGAAAAAAACAAAGGAACTAATTATTCATCGAATCAAACATTTGTGGCAGCTTTTGCACAATCCAAT
TCTGGAGACGTAACTCCCAATTTGTGGGGTCCAGCAGATGGTGTAAATGATTATGCCCGACAAAATACAATTGCAAACAA
ACAGTATCAAAAGGCAGTGAGTCTTTACCAGTCGGCAAATACGCAAGTGACTGGTACCATTGACTTTCGTCATACGTATG
TTAATTTTTCAAACTTGTATGTGAGTAGCGTTGGAACATCAACTTGTCCTGCCGGAATGGGAGCATCTTTTTCTGCGGGA
AGTGTGGAAGACAATGCAGTCTCCGTTGATTTTTTTGACGAAGGAACGACTGTTGATTCCTTGGATTGGAATTCCAATGC
AGCCGATGCATTCAAAGCAAGTTTTCTTGGTGGAGCCCTTGGCGTATTATGGCCAGCTTCTGTGAGTGAGTCCTACAAAT
TATGCCATGCTGAAAAACCAGTCCTCATTCCAACAGGTGTAGCTAGTTTTGATGGAAATCCTTGGACACCTCCTATCATC
CCAATTCAAATTGTAAAGATTGGAAATTTATCCATTCTTGCGATCCCAGCAGAAGTATCCACGATGGCTGGAAGACGGAT
TCGTTCTCTTGTCAAAAACATTATGGAAAATGATTACACTGTCATTGCAGCACTTTCCAATTCCTATACATCCTATCTCA
CAACGAGAGAAGAGTATTCTTCTCAGCAGTATGAAGGGGCATCAACTCAGTTTGGTCCTAATACTTTAAAAGCCTACGAA
CAAGAATTTGGTAAGTTAGCATCGGCATTACGAAATGGTGCAACGGTTCCAGGCGGACCTACACCTCCTGATCTTACAAA
CAACCAAGCTACCTTTCAAACGGGAGTGGTGTTTGATGATGTTCCCCTCTTTAAAAGTTTTGGGAATGTATTCACACAAC
CGTCAGCAACCTATGCAAGTGGTGCAAAGGTAACAGCTGTTTTCTGGGGAGCTCATCCAAAAAATAATATGTTGATCGGG
AGTAGTTTTGTGGATATTGAACGCCAAAATGGATCCACATGGACTGTTGTGGCTCGCGACAATGACCCTTCGACCACTTA
TAAATGGCAAAGAGATGGGATCGCTTATTCCAAAATTACAACCACTTGGGATACAACCTCCTATCCAAAAGGTACTTACC
GCATTCGCCATCGAGGACATTGGAAATCGGGATGGACAGGAGCGATTAGTTCCTACCAAGGCGTGACAAACAATTTTGTA
ATCCAATAG

Upstream 100 bases:

>100_bases
CATCGTTTAGACTTAAGTTCCTGATTTGACCCAAGGGTTCCCTTCGGACAGATTGATGCGAAAAATGTTAGATTGAGAAA
TTTAGATTCGGAGAGAGTAT

Downstream 100 bases:

>100_bases
GAATATCTGAACAATCACCTCGCCTGACCCCTAAAGGGTCAGGTATTTTTCTTTGCAACGGGATTGACTGGGTGAGATTC
CTTAAGATGCTCGTACTATG

Product: putative neutral/alkaline ceramidase

Products: NA

Alternate protein names: N-CDase; NCDase; Acylsphingosine deacylase; N-acylsphingosine amidohydrolase [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MNSQISPLVRVSVSIVTCLFVLACSDKKPSQSNTAILGLVGADQVESSKADNADLLFSSGVNRAVAPSLGSSPYLVGVGI
SDITGPAAEVGMMGFAETAQKTEGIYMRLWSRAYIIGDASKRVVFVSADLGMIFQSIKQAVSKKIATDSELAPFYSEANV
LLSATHTHSGPGGYSHYFLYNATTSGFIKENFDVIVNGIYKSIKLAHQNLVPGNVYVNQGELTDASKNRSVAAYDKNPAS
ERNFYPSNVDQTMTLLKLVAADGRELGMVNWFAVHPTNVGPTNKLIGGDNKGIASYLFEKNKGTNYSSNQTFVAAFAQSN
SGDVTPNLWGPADGVNDYARQNTIANKQYQKAVSLYQSANTQVTGTIDFRHTYVNFSNLYVSSVGTSTCPAGMGASFSAG
SVEDNAVSVDFFDEGTTVDSLDWNSNAADAFKASFLGGALGVLWPASVSESYKLCHAEKPVLIPTGVASFDGNPWTPPII
PIQIVKIGNLSILAIPAEVSTMAGRRIRSLVKNIMENDYTVIAALSNSYTSYLTTREEYSSQQYEGASTQFGPNTLKAYE
QEFGKLASALRNGATVPGGPTPPDLTNNQATFQTGVVFDDVPLFKSFGNVFTQPSATYASGAKVTAVFWGAHPKNNMLIG
SSFVDIERQNGSTWTVVARDNDPSTTYKWQRDGIAYSKITTTWDTTSYPKGTYRIRHRGHWKSGWTGAISSYQGVTNNFV
IQ

Sequences:

>Translated_722_residues
MNSQISPLVRVSVSIVTCLFVLACSDKKPSQSNTAILGLVGADQVESSKADNADLLFSSGVNRAVAPSLGSSPYLVGVGI
SDITGPAAEVGMMGFAETAQKTEGIYMRLWSRAYIIGDASKRVVFVSADLGMIFQSIKQAVSKKIATDSELAPFYSEANV
LLSATHTHSGPGGYSHYFLYNATTSGFIKENFDVIVNGIYKSIKLAHQNLVPGNVYVNQGELTDASKNRSVAAYDKNPAS
ERNFYPSNVDQTMTLLKLVAADGRELGMVNWFAVHPTNVGPTNKLIGGDNKGIASYLFEKNKGTNYSSNQTFVAAFAQSN
SGDVTPNLWGPADGVNDYARQNTIANKQYQKAVSLYQSANTQVTGTIDFRHTYVNFSNLYVSSVGTSTCPAGMGASFSAG
SVEDNAVSVDFFDEGTTVDSLDWNSNAADAFKASFLGGALGVLWPASVSESYKLCHAEKPVLIPTGVASFDGNPWTPPII
PIQIVKIGNLSILAIPAEVSTMAGRRIRSLVKNIMENDYTVIAALSNSYTSYLTTREEYSSQQYEGASTQFGPNTLKAYE
QEFGKLASALRNGATVPGGPTPPDLTNNQATFQTGVVFDDVPLFKSFGNVFTQPSATYASGAKVTAVFWGAHPKNNMLIG
SSFVDIERQNGSTWTVVARDNDPSTTYKWQRDGIAYSKITTTWDTTSYPKGTYRIRHRGHWKSGWTGAISSYQGVTNNFV
IQ
>Mature_722_residues
MNSQISPLVRVSVSIVTCLFVLACSDKKPSQSNTAILGLVGADQVESSKADNADLLFSSGVNRAVAPSLGSSPYLVGVGI
SDITGPAAEVGMMGFAETAQKTEGIYMRLWSRAYIIGDASKRVVFVSADLGMIFQSIKQAVSKKIATDSELAPFYSEANV
LLSATHTHSGPGGYSHYFLYNATTSGFIKENFDVIVNGIYKSIKLAHQNLVPGNVYVNQGELTDASKNRSVAAYDKNPAS
ERNFYPSNVDQTMTLLKLVAADGRELGMVNWFAVHPTNVGPTNKLIGGDNKGIASYLFEKNKGTNYSSNQTFVAAFAQSN
SGDVTPNLWGPADGVNDYARQNTIANKQYQKAVSLYQSANTQVTGTIDFRHTYVNFSNLYVSSVGTSTCPAGMGASFSAG
SVEDNAVSVDFFDEGTTVDSLDWNSNAADAFKASFLGGALGVLWPASVSESYKLCHAEKPVLIPTGVASFDGNPWTPPII
PIQIVKIGNLSILAIPAEVSTMAGRRIRSLVKNIMENDYTVIAALSNSYTSYLTTREEYSSQQYEGASTQFGPNTLKAYE
QEFGKLASALRNGATVPGGPTPPDLTNNQATFQTGVVFDDVPLFKSFGNVFTQPSATYASGAKVTAVFWGAHPKNNMLIG
SSFVDIERQNGSTWTVVARDNDPSTTYKWQRDGIAYSKITTTWDTTSYPKGTYRIRHRGHWKSGWTGAISSYQGVTNNFV
IQ

Specific function: Hydrolyzes the ceramides into sphingosine and free fatty acid at an optimal pH of 8-9 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral ceramidase family [H]

Homologues:

Organism=Homo sapiens, GI221218981, Length=675, Percent_Identity=36.7407407407407, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI221307475, Length=652, Percent_Identity=36.5030674846626, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651405, Length=723, Percent_Identity=33.8865836791148, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651403, Length=723, Percent_Identity=33.8865836791148, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651401, Length=723, Percent_Identity=33.8865836791148, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651397, Length=723, Percent_Identity=33.8865836791148, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651399, Length=723, Percent_Identity=33.8865836791148, Blast_Score=390, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006823 [H]

Pfam domain/function: NA

EC number: =3.5.1.23 [H]

Molecular weight: Translated: 77715; Mature: 77715

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQISPLVRVSVSIVTCLFVLACSDKKPSQSNTAILGLVGADQVESSKADNADLLFSSG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHCCCCCCCCEEEEECC
VNRAVAPSLGSSPYLVGVGISDITGPAAEVGMMGFAETAQKTEGIYMRLWSRAYIIGDAS
CCCCCCCCCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECEEEEEECCC
KRVVFVSADLGMIFQSIKQAVSKKIATDSELAPFYSEANVLLSATHTHSGPGGYSHYFLY
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEE
NATTSGFIKENFDVIVNGIYKSIKLAHQNLVPGNVYVNQGELTDASKNRSVAAYDKNPAS
ECCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCC
ERNFYPSNVDQTMTLLKLVAADGRELGMVNWFAVHPTNVGPTNKLIGGDNKGIASYLFEK
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHEEC
NKGTNYSSNQTFVAAFAQSNSGDVTPNLWGPADGVNDYARQNTIANKQYQKAVSLYQSAN
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TQVTGTIDFRHTYVNFSNLYVSSVGTSTCPAGMGASFSAGSVEDNAVSVDFFDEGTTVDS
CEEEEEEEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEC
LDWNSNAADAFKASFLGGALGVLWPASVSESYKLCHAEKPVLIPTGVASFDGNPWTPPII
CCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCEE
PIQIVKIGNLSILAIPAEVSTMAGRRIRSLVKNIMENDYTVIAALSNSYTSYLTTREEYS
EEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHEEEHHHHC
SQQYEGASTQFGPNTLKAYEQEFGKLASALRNGATVPGGPTPPDLTNNQATFQTGVVFDD
CHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCEECC
VPLFKSFGNVFTQPSATYASGAKVTAVFWGAHPKNNMLIGSSFVDIERQNGSTWTVVARD
CHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEECCEEEEEECCCCEEEEEEEC
NDPSTTYKWQRDGIAYSKITTTWDTTSYPKGTYRIRHRGHWKSGWTGAISSYQGVTNNFV
CCCCCCEEEECCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCEE
IQ
EC
>Mature Secondary Structure
MNSQISPLVRVSVSIVTCLFVLACSDKKPSQSNTAILGLVGADQVESSKADNADLLFSSG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHCCCCCCCCEEEEECC
VNRAVAPSLGSSPYLVGVGISDITGPAAEVGMMGFAETAQKTEGIYMRLWSRAYIIGDAS
CCCCCCCCCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECEEEEEECCC
KRVVFVSADLGMIFQSIKQAVSKKIATDSELAPFYSEANVLLSATHTHSGPGGYSHYFLY
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEE
NATTSGFIKENFDVIVNGIYKSIKLAHQNLVPGNVYVNQGELTDASKNRSVAAYDKNPAS
ECCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCC
ERNFYPSNVDQTMTLLKLVAADGRELGMVNWFAVHPTNVGPTNKLIGGDNKGIASYLFEK
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHEEC
NKGTNYSSNQTFVAAFAQSNSGDVTPNLWGPADGVNDYARQNTIANKQYQKAVSLYQSAN
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TQVTGTIDFRHTYVNFSNLYVSSVGTSTCPAGMGASFSAGSVEDNAVSVDFFDEGTTVDS
CEEEEEEEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEC
LDWNSNAADAFKASFLGGALGVLWPASVSESYKLCHAEKPVLIPTGVASFDGNPWTPPII
CCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCEE
PIQIVKIGNLSILAIPAEVSTMAGRRIRSLVKNIMENDYTVIAALSNSYTSYLTTREEYS
EEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHEEEHHHHC
SQQYEGASTQFGPNTLKAYEQEFGKLASALRNGATVPGGPTPPDLTNNQATFQTGVVFDD
CHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCEECC
VPLFKSFGNVFTQPSATYASGAKVTAVFWGAHPKNNMLIGSSFVDIERQNGSTWTVVARD
CHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEECCEEEEEECCCCEEEEEEEC
NDPSTTYKWQRDGIAYSKITTTWDTTSYPKGTYRIRHRGHWKSGWTGAISSYQGVTNNFV
CCCCCCEEEECCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCEE
IQ
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10593963; 10984043 [H]