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The map label for this gene is hycE [H]

Identifier: 183221788

GI number: 183221788

Start: 2491225

End: 2492652

Strand: Reverse

Name: hycE [H]

Synonym: LEPBI_I2419

Alternate gene names: 183221788

Gene position: 2492652-2491225 (Counterclockwise)

Preceding gene: 183221789

Following gene: 183221787

Centisome position: 69.25

GC content: 36.48

Gene sequence:

>1428_bases
ATGAAAAAGATTACTGGTATAACAAATTTTAATGGAATTTATTATCAGTTTCACTTTGACAATCAGAAGCTGGTGATGGA
AGAACAAATTACTAAAAAAAGTAATATTGATTTTTTATTAGATTCTAATTATCCGATTTGGTTGATTCGCCATTCATTTG
GACAAGACATGGGTCCAGAAGATTATTCCGAAATAAAAGAGGATGACTTTTTAACAGACCAAAGAGGCAAAAATTTATCT
CTTCATCAAAAGACGGGTATCATAAGGGATTTATTTTACCATGGACTTAAAGTTCCTTTTTCGGATGGAGAATACTCTCA
TGCCGTTGGACCCATTCATGCTGGAATAATTGAGCCTGGACATTTCCGATTTATAGTCGATGGAGAAGTAATTCGTCATC
TAACAATTCGCCTTGGATTTCAAAAGAGGGGGATTCGAGATCATCTGCTTCATAAGGCGATGAATCAGGTCATGCCATAT
TCTGAAATGATTTCAGGAGATACAAGCGTTGGTTATGCATTAGCATTCTCTAAAATATATGAGGACTTATACGAGATTCC
TGTTTCAAAAGAAATTCGATTATTCCGTTCGATCTTATTGGAGTTGGAGCGAATTGCCGTTCATATTGGTGATTTGGGTG
GTATCTCTGAAGATATAGGCTATTATCCATTGTATGGTGTTTGTGTAACTGAAAGAGGTGCCGCATTAGGGCTAATGGAA
GCTTGGACAGGACATCGCTTCGGAAAATCAGTCGTTCGTCCAGGTAGGTTATATCTCAACAATCGATTGAATGCAAAACA
AGCCAAAGAGTCGTTCAAACAATTGAAAAATGCATACATTAAGAAGATTCGTCCGCAGATTCTCAGGGCTCTCTCGATTT
CGACTTTGAAGGAGAGAATGCAAGGATGTGGATTTATTTCTGAAATGGATGTCCAAAATTTTGGATTCGTAGGTATGGTA
GCAAGAATGGCTGGAGTGAATGAAGATTTACGAATTGAAAATTTTGATTACCCTAATTGGAATCCACTTCCTTTAAAAGA
AGAACACCATCATTATAATGGTGACGTTTGGGCAAGAATGTACATTCGTTATTCGGAAATTGAACAGTCAATGGATTGGA
TAGAGAAAACAATGGAAGAGATCAATTGGGATTTACTTTGGGAACCTAAAGTAAAGGTTCCCAATCAAGAAATCTTAACC
AAAAGAACAAAACCAGGTTTTTATTATTCTGCTGTCGAAGCCTGGCGAGGGCCATTGCTAGTAGCTTTGCAATTTGATGA
AAATGGATTAGTTAACAATTCTTATATTAGGGATCCATCTGTATTAAATTGGCATGCTTTGGAGTTGGCAGTACGTGGCG
AACAAGTAGCGGATTTTCCTTTAAACAATAAATCATTTAATTTAAGTTATGTTGGGTTTGATTTATGA

Upstream 100 bases:

>100_bases
ATCATATATTGCAGTTTCGATTTTATTTTTTTTGGTTTAGTTTGGTTTCTACTATCATTGTAGGATCATATGGTATGTAT
TATTTGATGGCGAAATATAT

Downstream 100 bases:

>100_bases
ATTTTCTTTTTGAATTTAAAAATTTTATTACAAAAAAAAATGTTCTTAACTTTGATAAGGTTTCTTTTGTTCATGCGAAC
AATCGTGGAATTCCGACTCC

Product: hydrogenase-4 subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit C/D; NDH-1 subunit C/D [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKKITGITNFNGIYYQFHFDNQKLVMEEQITKKSNIDFLLDSNYPIWLIRHSFGQDMGPEDYSEIKEDDFLTDQRGKNLS
LHQKTGIIRDLFYHGLKVPFSDGEYSHAVGPIHAGIIEPGHFRFIVDGEVIRHLTIRLGFQKRGIRDHLLHKAMNQVMPY
SEMISGDTSVGYALAFSKIYEDLYEIPVSKEIRLFRSILLELERIAVHIGDLGGISEDIGYYPLYGVCVTERGAALGLME
AWTGHRFGKSVVRPGRLYLNNRLNAKQAKESFKQLKNAYIKKIRPQILRALSISTLKERMQGCGFISEMDVQNFGFVGMV
ARMAGVNEDLRIENFDYPNWNPLPLKEEHHHYNGDVWARMYIRYSEIEQSMDWIEKTMEEINWDLLWEPKVKVPNQEILT
KRTKPGFYYSAVEAWRGPLLVALQFDENGLVNNSYIRDPSVLNWHALELAVRGEQVADFPLNNKSFNLSYVGFDL

Sequences:

>Translated_475_residues
MKKITGITNFNGIYYQFHFDNQKLVMEEQITKKSNIDFLLDSNYPIWLIRHSFGQDMGPEDYSEIKEDDFLTDQRGKNLS
LHQKTGIIRDLFYHGLKVPFSDGEYSHAVGPIHAGIIEPGHFRFIVDGEVIRHLTIRLGFQKRGIRDHLLHKAMNQVMPY
SEMISGDTSVGYALAFSKIYEDLYEIPVSKEIRLFRSILLELERIAVHIGDLGGISEDIGYYPLYGVCVTERGAALGLME
AWTGHRFGKSVVRPGRLYLNNRLNAKQAKESFKQLKNAYIKKIRPQILRALSISTLKERMQGCGFISEMDVQNFGFVGMV
ARMAGVNEDLRIENFDYPNWNPLPLKEEHHHYNGDVWARMYIRYSEIEQSMDWIEKTMEEINWDLLWEPKVKVPNQEILT
KRTKPGFYYSAVEAWRGPLLVALQFDENGLVNNSYIRDPSVLNWHALELAVRGEQVADFPLNNKSFNLSYVGFDL
>Mature_475_residues
MKKITGITNFNGIYYQFHFDNQKLVMEEQITKKSNIDFLLDSNYPIWLIRHSFGQDMGPEDYSEIKEDDFLTDQRGKNLS
LHQKTGIIRDLFYHGLKVPFSDGEYSHAVGPIHAGIIEPGHFRFIVDGEVIRHLTIRLGFQKRGIRDHLLHKAMNQVMPY
SEMISGDTSVGYALAFSKIYEDLYEIPVSKEIRLFRSILLELERIAVHIGDLGGISEDIGYYPLYGVCVTERGAALGLME
AWTGHRFGKSVVRPGRLYLNNRLNAKQAKESFKQLKNAYIKKIRPQILRALSISTLKERMQGCGFISEMDVQNFGFVGMV
ARMAGVNEDLRIENFDYPNWNPLPLKEEHHHYNGDVWARMYIRYSEIEQSMDWIEKTMEEINWDLLWEPKVKVPNQEILT
KRTKPGFYYSAVEAWRGPLLVALQFDENGLVNNSYIRDPSVLNWHALELAVRGEQVADFPLNNKSFNLSYVGFDL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789076, Length=371, Percent_Identity=25.3369272237197, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788832, Length=375, Percent_Identity=26.1333333333333, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17555284, Length=375, Percent_Identity=24.8, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24638644, Length=382, Percent_Identity=22.7748691099476, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221459469, Length=352, Percent_Identity=23.2954545454545, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR022885
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54960; Mature: 54960

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITGITNFNGIYYQFHFDNQKLVMEEQITKKSNIDFLLDSNYPIWLIRHSFGQDMGPE
CCCCCCCCCCCEEEEEEEECCCEEEHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCC
DYSEIKEDDFLTDQRGKNLSLHQKTGIIRDLFYHGLKVPFSDGEYSHAVGPIHAGIIEPG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCC
HFRFIVDGEVIRHLTIRLGFQKRGIRDHLLHKAMNQVMPYSEMISGDTSVGYALAFSKIY
CEEEEECHHHHHHHHHEECCHHCCHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHH
EDLYEIPVSKEIRLFRSILLELERIAVHIGDLGGISEDIGYYPLYGVCVTERGAALGLME
HHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCCCCEEEEEEECCCCHHHHHH
AWTGHRFGKSVVRPGRLYLNNRLNAKQAKESFKQLKNAYIKKIRPQILRALSISTLKERM
HHHCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGCGFISEMDVQNFGFVGMVARMAGVNEDLRIENFDYPNWNPLPLKEEHHHYNGDVWARM
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEE
YIRYSEIEQSMDWIEKTMEEINWDLLWEPKVKVPNQEILTKRTKPGFYYSAVEAWRGPLL
EEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCEEHHHHHHCCCCEE
VALQFDENGLVNNSYIRDPSVLNWHALELAVRGEQVADFPLNNKSFNLSYVGFDL
EEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCEEECCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKKITGITNFNGIYYQFHFDNQKLVMEEQITKKSNIDFLLDSNYPIWLIRHSFGQDMGPE
CCCCCCCCCCCEEEEEEEECCCEEEHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCC
DYSEIKEDDFLTDQRGKNLSLHQKTGIIRDLFYHGLKVPFSDGEYSHAVGPIHAGIIEPG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCC
HFRFIVDGEVIRHLTIRLGFQKRGIRDHLLHKAMNQVMPYSEMISGDTSVGYALAFSKIY
CEEEEECHHHHHHHHHEECCHHCCHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHH
EDLYEIPVSKEIRLFRSILLELERIAVHIGDLGGISEDIGYYPLYGVCVTERGAALGLME
HHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCCCCEEEEEEECCCCHHHHHH
AWTGHRFGKSVVRPGRLYLNNRLNAKQAKESFKQLKNAYIKKIRPQILRALSISTLKERM
HHHCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGCGFISEMDVQNFGFVGMVARMAGVNEDLRIENFDYPNWNPLPLKEEHHHYNGDVWARM
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEE
YIRYSEIEQSMDWIEKTMEEINWDLLWEPKVKVPNQEILTKRTKPGFYYSAVEAWRGPLL
EEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCEEHHHHHHCCCCEE
VALQFDENGLVNNSYIRDPSVLNWHALELAVRGEQVADFPLNNKSFNLSYVGFDL
EEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCEEECCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA