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The map label for this gene is hyfF [H]

Identifier: 183221789

GI number: 183221789

Start: 2492649

End: 2493857

Strand: Reverse

Name: hyfF [H]

Synonym: LEPBI_I2420

Alternate gene names: 183221789

Gene position: 2493857-2492649 (Counterclockwise)

Preceding gene: 183221790

Following gene: 183221788

Centisome position: 69.28

GC content: 32.59

Gene sequence:

>1209_bases
ATGATGACATATGTTTTTTATTTTTCCGGAATTCTAACTTTTGTTATTATTTTTTTAGTGTCTGTATTGGCGCCAACAAA
AGGCCAAACCAGGATCTGGTTATGGAGTTTGCTTAAAGTATGTTTTTTTGCTGTATTGTTTTACTCCTGGTTTACAGAGA
ATATTGTGTTAAAATGGATTTTGATCGAAGCATCAACGTTATTCGGTGCGTTACTCATTTCTTCGAGTGGGACAGAGCGA
TCTTTTCATGTAGGTTGGAAATTTTTACTCATTAATTCCTATGCACTAGGATTGGCATTTTTAGGAATCGTAATCCTTCT
CTTTGCATCAACGCCACTCGAGAATTTGGATTTTAATTCTCTTAAACAGGGATTAGTTGGTCAGTCTGGATTATTAGTGG
AGACTGGAATCTTGTTAACTATTTATGGTTATAGCGGGAAATTGGGTTTAGTTCCAAATCACTTTTGGGTAGGGGATACA
TATGCAGAGAGTCCAAGTCAAATCTCGTCTCTGATTGCTTCCTTTGTTCCAGTGAGTGTCGTTCTTGCCATTAGGCCATT
AGTTAAATTAGAAAGAGAATTAAATCCTCATTTGATTAATGCTTCGAATGGGTTATTGTTTATTGGTGTTTTGACGATTC
TCTATGCTATCTTGATGTTAGTATCAAGAGACGACATTCGAAGAATCTCTGCGAAAGTAGCTCTCTTTCATACAGGAATG
TTAACTTTGTTTTTATGGTTGGATGTTTCAGATGAAGTGTTTTACTTTTTATTAAGTTCAACAGTTTTAGTTAAACTACT
TGTTTTTTTATCGATGGGAATTTTAAGGATGGATGCGGGTAAAAGGAATATATCTCAAATTTTGAGTGGTCCAAACCTAA
GTCATAAAGCATTATATGTTTACTTGATGGCTTTGTTAATCGCATTTGTTTTTCCATTATCTCCTGTGTTTGTTTTAGAT
TTGAAAATAATTGAAATTTCAGTTTTGAATCGACAATTTTACCTTTTTATATTTCCAATTTTGGGTTCAATTTTCTTTTT
TGTGGCATTGAATAAAGTTTTGCCTATGGTTAAACTTTCGAATCGAAATTTTGAAAAAGATATTTATCATATATTGCAGT
TTCGATTTTATTTTTTTTGGTTTAGTTTGGTTTCTACTATCATTGTAGGATCATATGGTATGTATTATTTGATGGCGAAA
TATATATGA

Upstream 100 bases:

>100_bases
TCGGTTTGGAGTTCGGTTCATTTTTAGATGCCATATTGGTCATTGTTTCAGGTGGAATTTTACAGCTCTCGCCTCATATG
CATACTAAGGAAAGAAAATT

Downstream 100 bases:

>100_bases
AAAAGATTACTGGTATAACAAATTTTAATGGAATTTATTATCAGTTTCACTTTGACAATCAGAAGCTGGTGATGGAAGAA
CAAATTACTAAAAAAAGTAA

Product: hydrogenase-4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MMTYVFYFSGILTFVIIFLVSVLAPTKGQTRIWLWSLLKVCFFAVLFYSWFTENIVLKWILIEASTLFGALLISSSGTER
SFHVGWKFLLINSYALGLAFLGIVILLFASTPLENLDFNSLKQGLVGQSGLLVETGILLTIYGYSGKLGLVPNHFWVGDT
YAESPSQISSLIASFVPVSVVLAIRPLVKLERELNPHLINASNGLLFIGVLTILYAILMLVSRDDIRRISAKVALFHTGM
LTLFLWLDVSDEVFYFLLSSTVLVKLLVFLSMGILRMDAGKRNISQILSGPNLSHKALYVYLMALLIAFVFPLSPVFVLD
LKIIEISVLNRQFYLFIFPILGSIFFFVALNKVLPMVKLSNRNFEKDIYHILQFRFYFFWFSLVSTIIVGSYGMYYLMAK
YI

Sequences:

>Translated_402_residues
MMTYVFYFSGILTFVIIFLVSVLAPTKGQTRIWLWSLLKVCFFAVLFYSWFTENIVLKWILIEASTLFGALLISSSGTER
SFHVGWKFLLINSYALGLAFLGIVILLFASTPLENLDFNSLKQGLVGQSGLLVETGILLTIYGYSGKLGLVPNHFWVGDT
YAESPSQISSLIASFVPVSVVLAIRPLVKLERELNPHLINASNGLLFIGVLTILYAILMLVSRDDIRRISAKVALFHTGM
LTLFLWLDVSDEVFYFLLSSTVLVKLLVFLSMGILRMDAGKRNISQILSGPNLSHKALYVYLMALLIAFVFPLSPVFVLD
LKIIEISVLNRQFYLFIFPILGSIFFFVALNKVLPMVKLSNRNFEKDIYHILQFRFYFFWFSLVSTIIVGSYGMYYLMAK
YI
>Mature_402_residues
MMTYVFYFSGILTFVIIFLVSVLAPTKGQTRIWLWSLLKVCFFAVLFYSWFTENIVLKWILIEASTLFGALLISSSGTER
SFHVGWKFLLINSYALGLAFLGIVILLFASTPLENLDFNSLKQGLVGQSGLLVETGILLTIYGYSGKLGLVPNHFWVGDT
YAESPSQISSLIASFVPVSVVLAIRPLVKLERELNPHLINASNGLLFIGVLTILYAILMLVSRDDIRRISAKVALFHTGM
LTLFLWLDVSDEVFYFLLSSTVLVKLLVFLSMGILRMDAGKRNISQILSGPNLSHKALYVYLMALLIAFVFPLSPVFVLD
LKIIEISVLNRQFYLFIFPILGSIFFFVALNKVLPMVKLSNRNFEKDIYHILQFRFYFFWFSLVSTIIVGSYGMYYLMAK
YI

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=242, Percent_Identity=25.2066115702479, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45590; Mature: 45590

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTYVFYFSGILTFVIIFLVSVLAPTKGQTRIWLWSLLKVCFFAVLFYSWFTENIVLKWI
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEASTLFGALLISSSGTERSFHVGWKFLLINSYALGLAFLGIVILLFASTPLENLDFNS
HHHHHHHHHHHHHCCCCCCCEEECCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LKQGLVGQSGLLVETGILLTIYGYSGKLGLVPNHFWVGDTYAESPSQISSLIASFVPVSV
HHHHCCCCCCCEEECCEEEEEECCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
VLAIRPLVKLERELNPHLINASNGLLFIGVLTILYAILMLVSRDDIRRISAKVALFHTGM
HHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLFLWLDVSDEVFYFLLSSTVLVKLLVFLSMGILRMDAGKRNISQILSGPNLSHKALYV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHH
YLMALLIAFVFPLSPVFVLDLKIIEISVLNRQFYLFIFPILGSIFFFVALNKVLPMVKLS
HHHHHHHHHHHCCCCHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNFEKDIYHILQFRFYFFWFSLVSTIIVGSYGMYYLMAKYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MMTYVFYFSGILTFVIIFLVSVLAPTKGQTRIWLWSLLKVCFFAVLFYSWFTENIVLKWI
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEASTLFGALLISSSGTERSFHVGWKFLLINSYALGLAFLGIVILLFASTPLENLDFNS
HHHHHHHHHHHHHCCCCCCCEEECCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LKQGLVGQSGLLVETGILLTIYGYSGKLGLVPNHFWVGDTYAESPSQISSLIASFVPVSV
HHHHCCCCCCCEEECCEEEEEECCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
VLAIRPLVKLERELNPHLINASNGLLFIGVLTILYAILMLVSRDDIRRISAKVALFHTGM
HHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLFLWLDVSDEVFYFLLSSTVLVKLLVFLSMGILRMDAGKRNISQILSGPNLSHKALYV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHH
YLMALLIAFVFPLSPVFVLDLKIIEISVLNRQFYLFIFPILGSIFFFVALNKVLPMVKLS
HHHHHHHHHHHCCCCHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNFEKDIYHILQFRFYFFWFSLVSTIIVGSYGMYYLMAKYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA